Determining the regulons and their functions in hyperthermophiles

• Project/Area Number: 21350092
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Kyoto University
• Principal Investigator: ATOMI Haruyuki 京都大学, 大学院・工学研究科, 教授 (90243047)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2011: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 転写制御因子 / 遺伝子破壊 / regulon / 超好熱菌 / 始原菌 / ゲノム / Thermococcus kodakarensis / プロモーター / Thermococcus kodakaraensis / 機能未知遺伝子

Research Abstract

We have previously determined the complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakarensis, and have also established a gene disruption and transcriptome analysis system for this organism. Using this information/technology, we disrupted genes predicted to encode transcription regulation factors. This project has led to the elucidation of multiple regulons, and we have been able to propose the functions of genes in several regulons.

Research Products

• [Journal Article] Thermococcus kodakarensis as a host for gene expression and protein secretion (2011)
  • Author(s): Takemasa, R., Yokooji, Y., Yamatsu, A., Atomi, H., Imanaka, T.
  • Journal Title: Applied and Environmental Microbiology Volume: 77(7) Pages: 2392-2398
  • Peer Reviewed
• [Journal Article] Thermococcus kodakarensis as a host for gene expression and protein secretion (2011)
  • Author(s): Takemasa R, Yokooji Y, Yamatsu A, Atomi H, Imanaka T
  • Journal Title: Applied and Environmental Microbiology Volume: 77 Issue: 7 Pages: 2392-2398
  • DOI: 10.1128/aem.01005-10
  • Peer Reviewed
• [Journal Article] Identification of the Phr-dependent heat shock regulon in the hyperthermophilic arch eon, Thermococcus kodakaraensis (2010)
  • Author(s): Kanai, T., Takedomi, S., Fujiwara, S., Atomi, H., Imanaka, T.
  • Journal Title: Journal of Biochemistry Volume: 147(3) Pages: 361-370
  • Peer Reviewed
• [Journal Article] Thermococcus kodakarensis mutants deficient in di-myo-inositol phosphate use aspartate to cope with heat stress (2010)
  • Author(s): Borges, N., Matsumi, R., Imanaka, T., Atomi, H., Santos, H.
  • Journal Title: Journal of Bacteriology Volume: 192(1) Pages: 191-197
  • Peer Reviewed
• [Journal Article] Identification of the Phr-dependent heat shock regulon in the hyperthermophilic archaeon, Thermococcus kodakaraensis. (2010)
  • Author(s): Kanai, T., Takedomi, S., Fujiwara, S., Atomi, H., Imanaka, T.
  • Journal Title: Journal of Biochemistry Volume: 147 Pages: 361-370
  • NAID: 10027874436
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 跡見晴幸, 小宮山眞 監修
  • Journal Title: 酵素利用技術大系―基礎・解析から改変・高機能化・産業利用まで―(株式会社エヌ・ティー・エス) Pages: 510-517
• [Journal Article] Identification of the Phr-dependent heat shock regulon in the hyperther mophilic archaeon, Thermococcus kodakaraensis (2009)
  • Author(s): Kanai T, Takedomi S, Fujiwara S, Atomi H, Imanaka T
  • Journal Title: J.Biochem. 147(3) Pages: 361-370
  • Peer Reviewed
• [Journal Article] The Fur iron regulator-like protein is cryptic in the hyperthermophilic archaeaon Thermococcus kodakaraensis (2009)
  • Author(s): Louvel H, Kanai T, Atomi H, Reeve JN
  • Journal Title: FEMS Microbiol.Lett. 295 Pages: 117-128
  • Peer Reviewed
• [Presentation] 超好熱性アーキアThermococcus kodakarensisのゲノム配列からの新規転写制御因子の同定 (2011)
  • Author(s): 山本康之、金井保、跡見晴幸
  • Organizer: 極限環境微生物学会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-11-28
• [Presentation] 超好熱性アーキアThermococcus kodakarensisにおける糖代謝関連転写制御因子Tgrの機能解析 (2011)
  • Author(s): 小谷徹、金井保、跡見晴幸
  • Organizer: 極限環境微生物学会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-11-28
• [Presentation] Elucidation of the functions of the ArsR transcription regulators in Thermococcus kodakarensis (2011)
  • Author(s): Lee, S, Kanai, T, Atomi, H
  • Organizer: 極限環境微生物学会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-11-28
• [Presentation] The unique metabolic pathways of hyperthermophilic archaea (2011)
  • Author(s): Atomi, H, Imanaka, T
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-09-07
• [Presentation] 超好熱菌を宿主とした細胞工学の試み (2010)
  • Author(s): 跡見晴幸
  • Organizer: 第5回産業用酵素シンポジウム
  • Place of Presentation: 長浜バイオ大学(滋賀県)
  • Year and Date: 2010-11-06
• [Presentation] Novel metabolic enzymes from the hyperthermophilic archaeon Thermococcus kodakarensis. Extremophiles (2010)
  • Author(s): Haruyuki Atomi
  • Place of Presentation: ポンタデルガダ,ポルトガル
  • Year and Date: 2010-09-13
• [Presentation] Novel metabolic enzymes from the hyperthermophilic archaeon Thermosoccur kodakarensis (2010)
  • Author(s): 跡見晴幸
  • Organizer: Extremophiles, 2010
  • Place of Presentation: ポンタデルガダ、ポルトガル
  • Year and Date: 2010-09-13
• [Presentation] ゲノム情報を利用した微生物の新規機能酵素の探索 (2010)
  • Author(s): 跡見晴幸、横大路裕介、金井保、佐藤喬章、今中忠行
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-03-09
• [Presentation] 超好熱始原菌の環境応答機構 (2009)
  • Author(s): 跡見晴幸
  • Organizer: GCOE第4回国際シンポジウム
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2009-11-13
• [Presentation] 超好熱始原菌の環境応答機構 (転写制御、熱ショック蛋白質) (2009)
  • Author(s): 跡見晴幸
  • Organizer: GCOE第4回国際シンポジウム
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2009-11-13
• [Presentation] Novel enzymes of the archaea involved in coenzyme biosynthesis (2009)
  • Author(s): 跡見晴幸
  • Organizer: Gordon Research Conference 2009
  • Place of Presentation: New Hampshire, U. S. A.
  • Year and Date: 2009-07-29
• [Book] Protein Engineering Handbook vol.III (Enzymes from thermophilic organisms) (2012)
  • Author(s): Kanai, T, Atomi, H
  • Publisher: Wiley VCH(in press)
• [Book] 超好熱菌ゲノム情報に基づいた新規代謝酵素・経路の解明 (2010)
  • Author(s): 跡見晴幸、佐藤喬章、横大路裕介、今中忠行
  • Publisher: 株式会社エヌ・ティー・エス
• [Book] 酵素利用技術大系第5編第5章第2節「超好熱菌ゲノム情報に基づいた新規代謝酵素・経路の解明」 (2010)
  • Author(s): 跡見晴幸、佐藤喬章、横大路裕介、今中忠行
  • Publisher: 株式会社エヌ・ティー・エス 小宮山眞監修
• [Remarks]
  • URL: http://www.sbchem.kyoto-u.ac.jp/atomi-lab/