Development of novel functional oligonucleotide analogs for detection and regulation of a single base alteration in gene
| Project/Area Number |
21350093
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
MURAKAMI Akira 京都工芸繊維大学, 工芸科学研究科, 教授 (60210001)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAYOSHI Asako 京都工芸繊維大学, 工芸科学研究科, 助教 (70380532)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Shinya 佐賀大学, 医学部, 教授 (80359794)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | 遺伝子 / 核酸 / バイオテクノロジー / 細胞・組織 / 蛋白質 / 遺伝子診断 / 蛍光核酸プローブ / ピレン / 遺伝子治療 |
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| Research Abstract |
The congenital and acquired base mutations in genes are related to various diseases and to individual physical constitution. In this research, novel functional nucleic acid analogs were synthesized for development of a novel detection system for a single point mutation in gene and for suppression of cancer cell proliferation. Following results were obtained ;(1) Synthesis of novel functional nucleic acid analogs for gene diagnosis and gene regulation ;(2) Development of a protocol for homogeneous fluorescent assay of base alteration in RUNX1 and JAK2 genes ;(3) Selective inhibition of cancer cell proliferation by photodynamic antisense protocol ;(4) Development of live-cell RNA-imaging protocol. Through this research, we are convinced that the nucleic acids with sophisticated functions can reveal unknown functions of" RNA".
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Report
(4 results)
Research Products
(106 results)
