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Creation and Structure-Function Control of Photo-Reactive Proteins and Peptides

Research Project

Project/Area Number 21350095
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionNara Institute of Science and Technology

Principal Investigator

HIROTA Shun  奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (90283457)

Co-Investigator(Kenkyū-buntansha) NAGAO Satoshi  奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (30452535)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords光応答性 / タンパク質 / ペプチド / 構造機能相関 / 機能制御 / 金属錯体 / タンパク質構造機能相関 / タンパク質機能制御 / ペプチド質機能制御
Research Abstract

Control of the structure and function of proteins and peptides with high space and time resolution will initiate new technologies, which include living body measurements and medical treatment. In this research, photo-reactive proteins and peptides were constructed, and photo-control of the structure and function of these biologically -related molecules was performed. The structure-function analysis of the protein with the use of its photoactivity , fixation of a photoactive ligand on a gold surface and regulation of its metal ion binding, and control of DNA cleavage with a photoactive ligand were performed.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (43 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (25 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Photosensitivity of the Ni-A State of [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F with Visible Light2013

    • Author(s)
      H. Osuka, Y. Shomura, H. Komori, N.Shibata, S. Nagao, Y. Higuchi, S. Hirota
    • Journal Title

      Biochem.Biophys.Res.Commun

      Volume: 430 Pages: 284-288

    • NAID

      120005716864

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photosensitivity of the Ni-A State of [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F with Visible Light2013

    • Author(s)
      H. Osuka, Y. Shomura, H. Komori, N. Shibata, S. Nagao, Y. Higuchi, S. Hirota
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 430 Issue: 1 Pages: 284-288

    • DOI

      10.1016/j.bbrc.2012.10.136

    • NAID

      120005716864

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA Cleavage by the Photocontrolled Cooperation of ZnII Centers in an Azobenzene-Linked Dizinc Complex2011

    • Author(s)
      A. Panja, T. Matsuo, S. Nagao, S. Hirota
    • Journal Title

      Inorg. Chem

      Volume: 50 Pages: 11437-11445

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient Reduction of Cys110 Thiyl Radical by Glutathione in Human Myoglobin2011

    • Author(s)
      S. Nagao, O. Asami, H. Yasui, S. Hirota
    • Journal Title

      Biochim. Biophys. Acta Proteins and Proteomics

      Volume: 1814 Pages: 480-486

    • NAID

      120005716866

    • Related Report
      2012 Final Research Report
  • [Journal Article] Efficient Reduction of Cys110 Thiyl Radical by Glutathione in Human Myoglobin2011

    • Author(s)
      S.Nagao, O.Asami, H.Yasui, S.Hirota
    • Journal Title

      Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics

      Volume: 1814 Issue: 4 Pages: 480-486

    • DOI

      10.1016/j.bbapap.2011.01.008

    • NAID

      120005716866

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA Cleavage by the Photocontrolled Cooperation of Zn^<II> Centers in an Azobenzene-Linked Dizinc Complex2011

    • Author(s)
      A.Panja, T.Matsuo, S.Nagao, S.Hirota
    • Journal Title

      Inorganic Chemistry

      Volume: 50 Issue: 22 Pages: 11437-11445

    • DOI

      10.1021/ic201244y

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Basis of the Lactate-Dependent Allosteric Regulation of Oxygen Binding in Arthropod Hemocyanin2011

    • Author(s)
      S.Hirota, N.Tanaka, I.Micetic, P.Di Muro, S.Nagao, H.Kitagishi, K.Kano, R.S.Magliozzo, J.Peisach, M.Beltramini, L.Bubacco
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 19338-19345

    • NAID

      120005716868

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytochrome c Polymerization by Successive Domain Swapping at the C-Terminal Helix2011

    • Author(s)
      S.Hirota, Y.Hattori, S.Nagao, M.Taketa, H.Komori, H.Kamikubo, Z.Wang, I.Takahashi, S.Negi, Y.Sugiura, M.Kataoka, Y.Higuchi
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 107 Pages: 12854-12859

    • NAID

      120005716875

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytochrome cPolymerization by Successive Domain Swapping at the C-Terminal Helix2010

    • Author(s)
      S. Hirota, Y. Hattori, S. Nagao, M. Taketa, H. Komori, H. Kamikubo, Z. Wang, I. Takahashi, S. Negi, Y. Sugiura, M. Kataoka, Y. Higuchi
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 107 Pages: 12854-12859

    • NAID

      120005716875

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural Basis of the Lactate-Dependent Allosteric Regulation of Oxygen Binding in Arthropod Hemocyanin2010

    • Author(s)
      S. Hirota, N.Tanaka, I. Micetic, P. Di Muro,S. Nagao, H. Kitagishi, K. Kano, R. S. Magliozzo, J. Peisach, M. Beltramini, L. Bubacco
    • Journal Title

      J. Biol. Chem

      Volume: 285 Pages: 19338-19345

    • NAID

      120005716868

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulating Copper-Binding Affinity with Photoisomerizable Azobenzene Ligand by Construction of a Self-Assembled Monolayer2009

    • Author(s)
      I. Takahashi, Y. Honda, S. Hirota
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 48 Pages: 6065-6068

    • NAID

      120005716863

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of Protein-Ligand Interactions by Photocleavage of a Cyclic Peptide using Phosphatidylinositol 3-Kinase SH3 Domain as Model System2009

    • Author(s)
      I.Takahashi, S.Kuroiwa, H.Lindfors, L.Ndamba, A.Hiruma, T.Yajima, N.Okishio, M.Ubbink, S.Hirota
    • Journal Title

      Journal of peptide Science 15

      Pages: 411-416

    • NAID

      120005716872

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulating Copper-Binding Affinity with Photoisomerizable Azobenzene Ligand by Construction of a Self-Assembled Monolayer2009

    • Author(s)
      I.Takahashi, Y.Honda, S.Hirota
    • Journal Title

      Angewandte Chemie International Edition 48

      Pages: 6065-6068

    • NAID

      120005716863

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Structural Changes of Metalloproteinsand Metal-Peptide Complexes2012

    • Author(s)
      Shun Hirota
    • Organizer
      Third Dalton Transactions International Symposium: Bioinorganic Chemistry
    • Place of Presentation
      Suita
    • Year and Date
      2012-11-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Structural Changes of Metalloproteins and Metal-Peptide Complexes2011

    • Author(s)
      Shun Hirota
    • Organizer
      3rd Dalton Transactions International Symposium : Bioinorganic Chemistry
    • Place of Presentation
      大阪大学(吹田市)(招待講演)
    • Year and Date
      2011-11-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Investigation and Regulation of Protein and Peptide Structural Changes2011

    • Author(s)
      Shun Hirota
    • Organizer
      Japanese-German Scientific Cooperation in the 21st Century: Biocatalysts for Future Feedstock Utilization
    • Place of Presentation
      Nara
    • Year and Date
      2011-04-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Protein and Peptide Structural Changes: Protein Aggregation and PhotoactiveBiomaterials2010

    • Author(s)
      Shun Hirota
    • Organizer
      Nanotechnology and Medical Sciences(ICNMS-2010)
    • Place of Presentation
      Kolhapur, India
    • Year and Date
      2010-10-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Protein and Peptide Structural Changes : Protein Aggregation and Photoactive Biomaterials2010

    • Author(s)
      Shun Hirota
    • Organizer
      Nanotechnology and Medical Sciences (ICNMS-2010)
    • Place of Presentation
      D.Y.Patil University (Kolhapur, India)
    • Year and Date
      2010-10-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Photoregulation of Copper-Binding Affinity to the Azobenzene Ligand on Au Surface2010

    • Author(s)
      Isao Takahashi, Yuichiro Honda, Shun Hirota, Miki Hasegawa
    • Organizer
      International Conference on Nanoscopic Colloid and Surface Science
    • Place of Presentation
      Chiba
    • Year and Date
      2010-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Peptides, and Inorganic Compounds Incorporated with Photo-Triggering and Photo-Regulating Properties2010

    • Author(s)
      Shun Hirota, Isao Takahashi, Halan Prakash, Satoshi Nagao, Proteins
    • Organizer
      5th International Symposium on Macrocyclic and Supramolecular Chemistry (ISMSC 2010)
    • Place of Presentation
      Nara, Japan
    • Year and Date
      2010-06-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Protein Supramolecules and Photoactive Bio-related Compounds2010

    • Author(s)
      Shun Hirota
    • Organizer
      2nd WCU Symposium on Nanobio Materials and Electronics (WCU-02)
    • Place of Presentation
      Gwangju Institute of Science and Technology (Gwangju, Korea)
    • Year and Date
      2010-04-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 酸化型[NiFe]ヒドロゲナーゼにおける光反応性の研究2010

    • Author(s)
      大須賀久織、庄村康人、小森博文、柴田直樹、樋口芳樹、廣田俊
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      東大阪
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光異性化可能なアゾベンゼン配位子の表面固定による銅イオンの結合制御2010

    • Author(s)
      高橋勇雄、本田裕一郎、廣田俊、長谷川美貴
    • Organizer
      第22回配位化合物の光化学討論会
    • Place of Presentation
      富山市
    • Related Report
      2012 Final Research Report
  • [Presentation] Investigation and Regulation of Proteinand Peptide Structural Changes2009

    • Author(s)
      Shun Hirota
    • Organizer
      International Symposium on Protein Structures: Stability
    • Place of Presentation
      Interaction and Dynamics, Nara
    • Year and Date
      2009-12-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Proteins, Peptides and Inorganic Compounds Incorporated with Photo-Triggering and Photo-Regulating Properties2009

    • Author(s)
      Shun Hirota
    • Organizer
      The 9th GIST/NAIST JointSymposium on Advanced Materials
    • Place of Presentation
      Gwangju, Korea
    • Year and Date
      2009-11-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Peptides and Inorganic Compounds Incorporated with Photo-Triggering and Photo-Regulating Properties2009

    • Author(s)
      Shun Hirota, Isao Takahashi, Halan Prakash, Yuichiro Honda, Satoshi Nagao, Proteins
    • Organizer
      The 1st NCTU-NAIST Workshop on "Molecular/Nano Science" 2009
    • Place of Presentation
      Hsinchu, Taiwan
    • Year and Date
      2009-11-13
    • Related Report
      2012 Final Research Report
  • [Presentation] [NiFe] ヒドロゲナーゼのX線結晶構造解析と赤外分光測定(X-ray crystallographic and FT-IR studies on [NiFe] hydrogenase)2009

    • Author(s)
      Hisao Osuka, Shin-ichi Terawaki, Yasuhito Shomura, Hirofumi Komori, Naoki Shibata, Shun Hirota, Yoshiki Higuchi
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima
    • Year and Date
      2009-10-30
    • Related Report
      2012 Final Research Report
  • [Presentation] アゾベンゼン配位子の表面固定を利用した銅イオンの結合制御2009

    • Author(s)
      高橋勇雄、本田裕一朗、廣田俊
    • Organizer
      第59回錯体化学討論会
    • Place of Presentation
      長崎市
    • Year and Date
      2009-09-25
    • Related Report
      2012 Final Research Report
  • [Presentation] アゾベンゼン配位子の表面固定を利用した銅イオンの結合制御2009

    • Author(s)
      高橋勇雄、本田裕一郎、廣田俊
    • Organizer
      第59回錯体化学討論会
    • Place of Presentation
      長崎
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 節足動物由来ヘモシアニンの酸素結合挙動に対するpHおよび乳酸の影響2009

    • Author(s)
      廣田俊、田中直輝、長尾聡、北岸宏亮、加納航冶、I. Micetic、M. Bertamini、L.Bubacco
    • Organizer
      第36回生体分子科学討論会
    • Place of Presentation
      札幌市
    • Year and Date
      2009-06-19
    • Related Report
      2012 Final Research Report
  • [Presentation] Photoregulated Metal Binding to the Azobenzene Ligand on Au Surface2009

    • Author(s)
      Isao Takahashi, Yuichiro Honda, Shun Hirota
    • Organizer
      14th International Conference on Biological Inorganic Chemistry (ICBIC14)
    • Place of Presentation
      Nagoya
    • Related Report
      2012 Final Research Report
  • [Presentation] Bridging Ligand at the Active Site of Oxidized [NiFe] Hydrogenase2009

    • Author(s)
      Hisao Osuka, Shun Hirota, Shin-ichi Terawaki, Yasuhito Shomura, Hirofumi Komori, Naoki Shibata, Yoshiki Higuchi
    • Organizer
      14th International Conference on Biological Inorganic Chemistry (ICBIC14)
    • Place of Presentation
      Nagoya
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect of Cysteine Residue on Reaction of HumanMyoglobin with Hydrogen Peroxide2009

    • Author(s)
      Satoshi Nagao, Osamu Asami, Shun Hirota
    • Organizer
      14th International Conference on Biological Inorganic Chemistry (ICBIC14)
    • Place of Presentation
      Nagoya
    • Related Report
      2012 Final Research Report
  • [Presentation] Structural Origin for the Bohr Effect in Arthropod Hemocyanin2009

    • Author(s)
      Shun Hirota, Takumi Kawahara, Mariano Beltramini, Paolo Di Muro, Richard S. Magliozzo, Jack Peisach, Linda S. Powers,Naoki Tanaka, Satoshi Nagao, Luigi Bubacco
    • Organizer
      14th International Conference on Biological Inorganic Chemistry (ICBIC14)
    • Place of Presentation
      Nagoya
    • Related Report
      2012 Final Research Report
  • [Presentation] Photoregulated Metal Binding to the Azobenzene Ligand on Au Surface2009

    • Author(s)
      I.Takahashi, Y.Honda, S.Hirota
    • Organizer
      14th International Conference on Biological Inorganic chemistry (ICBIC14)
    • Place of Presentation
      Nagoya
    • Related Report
      2009 Annual Research Report
  • [Presentation] ドメインスワッピングを利用したヘムタンパク質の多量体形成

    • Author(s)
      長尾聡、富岡勇也、諸井麻希、廣田俊
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 金表面上のアゾベンゼン配位子からのCuの解離過程の考察

    • Author(s)
      高橋勇雄、小玉大典、廣田俊、長谷川美貴
    • Organizer
      第62回錯体化学討論会
    • Place of Presentation
      富山大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ドメインスワッピングを利用した亜鉛置換シトクロムcの多量体形成

    • Author(s)
      長尾聡、富岡勇也、諸井麻希、廣田俊
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学
    • Related Report
      2012 Annual Research Report
  • [Book] 光で生体関連分子の構造を制御する、現代化学10月号2010

    • Author(s)
      廣田俊
    • Publisher
      東京化学同人
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://mswebs.naist.jp/LABs/hirota/index.html

    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] リンカーを有するアゾ化合物及び当該アゾ化合物を用いた金属イオン回収方法2013

    • Inventor(s)
      廣田俊、高橋勇雄、本田裕一朗
    • Industrial Property Rights Holder
      奈良先端科学技術大学院大学
    • Acquisition Date
      2013-03-08
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] アゾペプチド複合体2013

    • Inventor(s)
      廣田俊、ハランプラカシュ
    • Industrial Property Rights Holder
      奈良先端科学技術大学院大学
    • Acquisition Date
      2013-02-08
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 光制御ペプチド及び光制御ペプチドを用いたペプチド-蛋白質複合体形成の制御方法2010

    • Inventor(s)
      廣田俊、濱崎勇二
    • Industrial Property Rights Holder
      奈良先端科学技術大学院大学
    • Acquisition Date
      2010-12-17
    • Related Report
      2012 Final Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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