Modification of enzyme functions by integration of conformationalinformation and function and sequence

• Project/Area Number: 21350096
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Keio University
• Principal Investigator: MIYAMOTO Kenji 慶應義塾大学, 理工学部, 准教授 (60360111)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: アリールマロン酸脱炭酸酵素 / ラセマーゼ / 機能改変 / 活性向上 / 立体構造 / アリールマロン酸脱炭酸素

Research Abstract

In this application, a novel arylmalonate decarboxylase (AMDase)was used to be a model enzyme. Conformational information on various enzymes isassociated with sequence information. The relativity of the structure and the function witha lot of uncertain po

Research Products

• [Journal Article] Engineering the PromiscuousRacemase Activity of an ArylmalonateDecarboxylase (2011)
  • Author(s): R. Kourist, Y . Miyauchi, D. Uemura and K.Miyamoto
  • Journal Title: Chemistry-A EuropeanJournal Volume: 17 Pages: 557-563
  • Peer Reviewed
• [Journal Article] ArylmalonateDecarboxylase G74C, an artificialracemase (2011)
  • Author(s): R. Kourist and K. Miyamoto
  • Journal Title: Biotech international Volume: 23 Pages: 6-11
• [Journal Article] アリールマロン酸脱炭酸酵素の機能改変 (2011)
  • Author(s): 宮本憲二
  • Journal Title: 酵素工学研究会「酵素工学ニュース」トピックス Volume: 64 Pages: 5-10
• [Journal Article] Biocatalytic strategies for theasymmetric synthesis of profens - recenttrends and developments (2011)
  • Author(s): R. Kourist, P . Dominguez de Maria and K.Miyamoto
  • Journal Title: GreenChemistry Volume: 13 Pages: 2607-2618
  • Peer Reviewed
• [Journal Article] Dramatically improvedcatalytic activity of an artificial(S)-selective arylmalonatedecarboxylase by structure-guideddirected evolution (2011)
  • Author(s): Y . Miyauchi, R. Kourist, D. Uemura and K.Miyamoto
  • Journal Title: ChemicalCommunications Volume: 47 Pages: 7503-7505
  • Peer Reviewed
• [Journal Article] Biocatalytic strategies for the asymmetric synthesis of profens-recent trends and developments (2011)
  • Author(s): R.Kourist, P.Dominguez de Maria, K.Miyamoto
  • Journal Title: Green Chemistry Volume: 13 Issue: 10 Pages: 2607-2618
  • DOI: 10.1039/c1gc15162b
  • Peer Reviewed
• [Journal Article] Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution (2011)
  • Author(s): Y.Miyauchi, R.Kourist, D.Uemura, K.Miyamoto
  • Journal Title: Chemical Communications Volume: 47 Issue: 26 Pages: 7503-7505
  • DOI: 10.1039/c1cc11953b
  • Peer Reviewed
• [Journal Article] Engineering the Promiscuous Racemase Activity of an Arylmalonate Decarboxylase (2011)
  • Author(s): R.Kourist, Y.Miyauchi, D.Uemura, K.Miyamoto
  • Journal Title: Chemistry-A European Joumal Volume: 17 Pages: 557-563
  • Peer Reviewed
• [Journal Article] Protein engineering of Arylmalonate Decarboxylase G74C, an artificial race mase (2011)
  • Author(s): R.Kourist, K.Miyamoto
  • Journal Title: Biotech international Volume: 23 Pages: 6-11
• [Presentation] 合理的デザインによる変異酵素の劇的活性向上 (2013)
  • Author(s): 榎純一、 宮内祐介、 吉田昭介、 宮本憲二
  • Organizer: 日本化学会第93 春季年会(2013)
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
• [Presentation] 合理的デザインによる変異酵素の劇的活性向上 (2013)
  • Author(s): 榎純一、宮内祐介、吉田昭介、宮本憲二
  • Organizer: 日本化学会第93春季年会(2013)
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
• [Presentation] Directed evolution によるアリールマロン酸脱炭酸酵素の酵素活性向上 (2012)
  • Author(s): 榎純一、 宮内祐介、 吉田昭介、 宮本憲二
  • Organizer: 第16回生体触媒化学シンポジウム
  • Place of Presentation: 富山県民会館
  • Year and Date: 2012-11-29
• [Presentation] 自然界に存在しない酵素を創りだし (2012)
  • Author(s): 宮本憲二
  • Organizer: 性能を向上する、新規素材探索研究会
  • Place of Presentation: 新横浜フジビューホテル(新横浜)
  • Year and Date: 2012-06-08
• [Presentation] 好熱性古細菌由来エステラーゼを用いた不斉ドミノ型反応の開発と選択性の向上 (2012)
  • Author(s): 九門 孝志、和田 玲奈、吉田 昭介、宮本 憲二
  • Organizer: 日本化学会第92 春季年会(2012)
  • Place of Presentation: 慶應義塾大学(横浜)
  • Year and Date: 2012-03-27
• [Presentation] 非天然化合物に作用するラセマーゼの創製と機能向上 (2012)
  • Author(s): KOURIST Robert、 吉田 昭介、 宮本 憲二
  • Organizer: 日本化学会第92 春季年会(2012)
  • Place of Presentation: 慶應義塾大学(横浜)
  • Year and Date: 2012-03-27
• [Presentation] S 体選択的アリールマロン酸脱炭酸酵素変異体の活性向上 (2012)
  • Author(s): 榎 純一、宮内 祐介、吉田 昭介、宮本 憲二
  • Organizer: 日本化学会第92 春季年会(2012)
  • Place of Presentation: 慶應義塾大学(横浜)
  • Year and Date: 2012-03-27
• [Presentation] 非天然化合物に作用するラセマーゼの創製と機能向上 (2012)
  • Author(s): KOURIST Robert、吉田昭介、宮本憲二
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶應義塾大学(日吉・矢上キャンパス)
  • Year and Date: 2012-03-27
• [Presentation] S体選択的アリールマロン酸脱炭酸酵素変異体の活性向上 (2012)
  • Author(s): 榎純一、宮内祐介、吉田昭介、宮本憲二
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶應義塾大学(日吉・矢上キャンパス)
  • Year and Date: 2012-03-27
• [Presentation] 好熱性古細菌由来エステラーゼを用いた不斉ドミノ型反応の開発と選択性の向上 (2012)
  • Author(s): 九門孝志、和田玲奈、吉田昭介、宮本憲二
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶應義塾大学(日吉・矢上キャンパス)
  • Year and Date: 2012-03-27
• [Presentation] Dramatically improvedcatalytic activity of an artificial(S)-selective arylmalonatedecarboxylase by directed evolution (2012)
  • Author(s): Kenji Miyamoto
  • Organizer: Gordon research conference
  • Place of Presentation: Bryant University(USA)
• [Presentation] Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by directed evolution (2012)
  • Author(s): Kenji Miyamoto
  • Organizer: Gordon research conference (Biocatalyst)
  • Place of Presentation: Bryant University (USA)
• [Presentation] 活性部位の再デザインによるアリールマロン酸脱炭酸酵素変異体の機能向上 (2011)
  • Author(s): 宮内祐介、ロバート・コウリスト、榎純一、吉田昭介、宮本憲二
  • Organizer: 第15回生体触媒化学シンポジウム
  • Place of Presentation: 慶應義塾大学薬学部(芝共立キャンパス)
  • Year and Date: 2011-12-22
• [Presentation] 好熱性エステラーゼによるドミノ型反応の開発と立体選択性の向上 (2011)
  • Author(s): 九門孝志、和田玲奈、吉田昭介、宮本憲二
  • Organizer: 第15回生体触媒化学シンポジウム
  • Place of Presentation: 慶應義塾大学薬学部(芝共立キャンパス)
  • Year and Date: 2011-12-22
• [Presentation] Dramatically improvedcatalytic activity of an artificial(S)-selective arylmalonatedecarboxylase by structure-guideddirected evolution (2011)
  • Author(s): Kenji Miyamoto
  • Organizer: 16thJapanese-German Workshop onEnzyme T echnology
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2011-09-14
• [Presentation] Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution (2011)
  • Author(s): Kenji Miyamoto
  • Organizer: 16th Japanese-German Workshop on Enzyme Technology
  • Place of Presentation: 富山国際会議場(招待講演)
  • Year and Date: 2011-09-14
• [Presentation] 耐熱性古細菌由来エステラーゼを用いた不斉ドミノ型反応の開発と選択性の向上 (2011)
  • Author(s): 九門孝志、和田玲奈、ロバートコウリスト、上村大輔、宮本憲二
  • Organizer: 日本化学会第91 春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] アリールマロン酸脱炭酸酵素の機能改変によるラセマーゼの創製 (2011)
  • Author(s): ロバートコウリスト、上村大輔、宮本憲二
  • Organizer: 日本化学会第91 春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] アリールマロン酸脱炭酸酵素の機能改変によるラセマーゼの創製 (2011)
  • Author(s): ロバートコウリスト・上村大輔・宮本憲二
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] アリールマロン酸脱炭酸酵素の機能改変による活性の向上 (2011)
  • Author(s): 宮内祐介・ロバートコウリスト・上村大輔・宮本憲二
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] 耐熱性古細菌由来エステラーゼを用いた不斉ドミノ型反応の開発と選択性の向上 (2011)
  • Author(s): 九門孝志・和田玲奈・ロバートコウリスト・上村大輔・宮本憲二
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] Proteinengineering of ArylmalonateDecarboxylase G74C, acofactor-independent racemase (2010)
  • Author(s): R. Kourist, K. Miyamoto
  • Organizer: Pafichem2010
  • Place of Presentation: Hawai (USA)
  • Year and Date: 2010-12-18
• [Presentation] Structure-GuidedSite-directed Mutagenesis ofArylmalonate Decarboxylase (2010)
  • Author(s): Y . Miyauchi, R. Kourist, D. Uemura, K.Miyamoto
  • Organizer: Pafichem2010
  • Place of Presentation: Hawai (USA)
  • Year and Date: 2010-12-18
• [Presentation] Asymmetric Domino Reactionby an Esterase from Sulfolobus tokodaiiStrain 7 (2010)
  • Author(s): T . Kumon, R. Wada, K. Miyamoto, D.Uemura
  • Organizer: Pafichem2010
  • Place of Presentation: Hawai (USA)
  • Year and Date: 2010-12-18
• [Presentation] Protein engineering of Arylmalonate Decarboxylase G74C, a cofactor-independent racemase (2010)
  • Author(s): R.Kourist, K.Miyamoto
  • Organizer: Pafichem2010
  • Place of Presentation: Hawai (USA)
  • Year and Date: 2010-12-18
• [Presentation] Structure-Guided Site-directed Mutagenesis of Arylmalonate Decarboxylase (2010)
  • Author(s): Y.Miyauchi, R.Kourist, D.Uemura, K.Miyamoto
  • Organizer: Pafichem2010
  • Place of Presentation: Hawai (USA)
  • Year and Date: 2010-12-18
• [Presentation] Asymmetric Domino Reaction by an Esterase from Sulfolobus tokodaii Strain 7 (2010)
  • Author(s): T.Kumon, R.Wada, K.Miyamoto, D.Uemura
  • Organizer: Pafichem2010
  • Place of Presentation: Hawai (USA)
  • Year and Date: 2010-12-18
• [Presentation] アリールマロン酸脱炭酸酵素の機能改変 (2010)
  • Author(s): 宮内祐介、宮本憲二、太田博道、上村大輔
  • Organizer: 第14回生体触媒化学シンポジウム
  • Place of Presentation: グランシップ静岡 (静岡)
  • Year and Date: 2010-09-24
• [Presentation] アリールマロン酸脱炭酸酵素の機能改変 (2010)
  • Author(s): 宮内祐介、宮本憲二、太田博道、上村大輔
  • Organizer: 第14回生体触媒化学シンポジウム
  • Place of Presentation: グランシップ静岡(静岡)(口頭発表)
  • Year and Date: 2010-09-24
• [Presentation] Protein engineering of arylmalonatedecarboxylase variants with promiscuousracemising activity (2010)
  • Author(s): R. Kourist, Y . Miyauchi, K. Miyamoto
  • Organizer: Dechema Jahrestagung der Biotechnologen
  • Place of Presentation: Aachen (Germany)
  • Year and Date: 2010-09-22
• [Presentation] Protein engineering of arylmalonate decarboxylase variants with promiscuous racemising activity (2010)
  • Author(s): R.Kourist, Y.Miyauchi, K.Miyamoto
  • Organizer: Dechema Jahrestagung der Biotechnologen
  • Place of Presentation: Aachen (Germany)
  • Year and Date: 2010-09-22
• [Presentation] Protein engineering of arylmalonatedecarboxylase variants with promiscuousracemising activity (2010)
  • Author(s): R. Kourist, Y . Miyauchi, K. Miyamoto
  • Organizer: Biocat2010
  • Place of Presentation: Hamburg (Germany)
  • Year and Date: 2010-09-01
• [Presentation] Protein engineering of arylmalonate decarboxylase variants with promiscuous racemising activity (2010)
  • Author(s): R.Kourist, Y.Miyauchi, K.Miyamoto
  • Organizer: Biocat2010
  • Place of Presentation: Hamburg (Germany)
  • Year and Date: 2010-09-01
• [Presentation] AsymmetricDecarboxylation by ArylmalonateDecarboxylase and ThermostableEsterase (2010)
  • Author(s): Kenji Miyamoto
  • Organizer: Gordon Research Conference(Biocatalysis)
  • Place of Presentation: BryantUniversity (USA)
  • Year and Date: 2010-07-14
• [Presentation] Asymmetric Decarboxylation by Arylmalonate Decarboxylase and Thermostable Esterase (2010)
  • Author(s): Kenji Miyamoto
  • Organizer: Gordon Research Conference (Biocatalysis)
  • Place of Presentation: Bryant University (USA)(招待講演)
  • Year and Date: 2010-07-14
• [Presentation] 好熱性個最近由来エステラーゼを用いた新規ドミノ型反応の開発 (2010)
  • Author(s): 和田玲奈、太田博道、宮本憲二、上村大輔
  • Organizer: 日本化学会 第90春季年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2010-03-28
• [Presentation] 好熱性古細菌由来エステラーゼST0071を用いた新規ドミノ型反応の開発 (2009)
  • Author(s): 和田玲奈、太田博道、宮本憲二、上村大輔
  • Organizer: 第13回生体触媒化学シンポジウム
  • Place of Presentation: 香川大学
  • Year and Date: 2009-12-03
• [Presentation] Directed evolutionによるアリールマロン酸脱炭酸酵素の酵素活性向上
  • Author(s): 榎純一、宮内祐介、吉田昭介、宮本憲二
  • Organizer: 第16回生体触媒化学シンポジウム
  • Place of Presentation: 富山県民会館
• [Presentation] 自然界に存在しない酵素を創りだし、性能を向上する
  • Author(s): 宮本憲二
  • Organizer: 新規素材探索研究会
  • Place of Presentation: 新横浜フジビューホテル
  • Invited
• [Book] Asymmetric decarboxylation of arylmalonates and racemization of profens by arylmalonate decarboxylase and its variants, PracticalMethods for Biocatalysis andBiotransformations (2012)
  • Author(s): Kenji Miyamoto, Robert Kourist, ShosukeY oshida, Hiromichi Ohta
  • Publisher: WILEY
• [Book] Practical Methods for Biocatalysis and Biotransformations 2 (2012)
  • Author(s): Kenji Miyamoto, Robert Kourist, ShosukeYoshida, Hiromichi Ohta
  • Total Pages: 7
  • Publisher: WILEY
• [Remarks]
  • URL: http://www.bio.keio.ac.jp/labs/kmiyamoto/
• [Remarks] 宮本研究室ホームページ
  • URL: http://www.bio.keio.ac.jp/labs/kmiyamoto/policy.html
• [Remarks]
  • URL: http://www.bio.keio.ac.jp/labs/kmiyamoto/
• [Remarks]
  • URL: http://www.bio.keio.ac.jp/labs/kmiyamoto/
• [Patent(Industrial Property Rights)] 特許(PCT出願) (2012)
  • Inventor(s): 宮本憲二
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Filing Date: 2012-02-27
  • Overseas
• [Patent(Industrial Property Rights)] S 体選択的高活性型変異型アリールマロン酸脱炭酸酵素 (2011)
  • Inventor(s): 宮本憲二
  • Industrial Property Rights Holder: 慶應義塾
  • Filing Date: 2011
• [Patent(Industrial Property Rights)] S体選択的高活性型変異型アリールマロン酸脱炭酸酵素 (2011)
  • Inventor(s): 宮本憲二
  • Industrial Property Rights Holder: 慶應義塾
  • Industrial Property Number: 2011-043085
  • Filing Date: 2011