| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Research Abstract |
Structural chemistry involved in allosteric effects of heme proteins was investigated with visible and ultraviolet resonance Raman spectroscopy. About hemoglobin as a fundamental molecule, a quaternary structure of half-ligated state was quantitatively determined on the basis of inter-subunit interactions. As applications, information transduction pathway and its dynamics were elucidated with two oxygen sensor proteins, DOS and HemAT, and structural effects of effector binding was revelaed for an NO sensor protein, sGC. For respiration enzyme, proton pumping mechanism of a mammalian cytochrome c oxidase was examined.
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