Structural Chemistry on Information Transduction through Allosteric Effects in Heme Proteins
| Project/Area Number |
21350098
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | University of Hyogo |
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Principal Investigator |
KITAGAWA Teizo 兵庫県立大学, 大学院・生命理学研究科, 教授 (40029955)
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| Co-Investigator(Kenkyū-buntansha) |
OGURA Takashi 兵庫県立大学, 大学院・生命理学研究科, 教授 (70183770)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | 生物無機化学 / 振動分光学 / アロステリック効果 / ガスセンサー蛋白 / ヘモグロビン / 情報伝達 / 生体分子化学 / 生物物理 / 共鳴ラマン / ヘム蛋白質 / 紫外共鳴ラマン / 生体生命情報学 / 生体分子科学 / 紫外共鳴ラマン散乱 |
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| Research Abstract |
Structural chemistry involved in allosteric effects of heme proteins was investigated with visible and ultraviolet resonance Raman spectroscopy. About hemoglobin as a fundamental molecule, a quaternary structure of half-ligated state was quantitatively determined on the basis of inter-subunit interactions. As applications, information transduction pathway and its dynamics were elucidated with two oxygen sensor proteins, DOS and HemAT, and structural effects of effector binding was revelaed for an NO sensor protein, sGC. For respiration enzyme, proton pumping mechanism of a mammalian cytochrome c oxidase was examined.
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Report
(4 results)
Research Products
(143 results)
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[Journal Article] Nickel(II) complexes of TPA ligands with 6-phenyl substituents PhnTPA. Structure and H_2O_2-reactivity2010
Author(s)
Tano, T., Doi, Y., Inosako, M., Kunishita, A., Kubo, M., Ishimaru, H., Ogura, T., Sugimoto, H., Itoh, S.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A square-planar Ni(II) complex with an N_2S_2 donor set similar to the active centre of nickel-containing superoxide dismutase and its reaction with superoxide2010
Author(s)
Nakane, D., Kuwasako, S., Tsuge, M., Kubo, M., Funahashi, Y., Ozawa, T., Ogura, T., Masuda, H.
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Journal Title
Chem.Commun. 46
Pages: 2142-2144
Related Report
Peer Reviewed
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[Journal Article] Identification of the Fe-O_2 and the Fe=O Heme Species for Indoleamine 2, 3-Dioxygenase during Catalytic Turnover2010
Author(s)
Yanagisawa, S., Yotsuya, K., Hashiwaki, Y., Horitani, M., Sugimoto, H., Shiro, Y., Appelman, E.H., Ogura
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Journal Title
Chem.Lett. 39
Pages: 36-37
NAID
Related Report
Peer Reviewed
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[Journal Article] Spectroscopic Characterization of a Hydroperoxo-Heme Intermediate : Conversion of a Side-On Peroxo to an End-On Hydroperoxo Complex2009
Author(s)
Liu, J.G., Ohta, T., Yamaguchi, S., Ogura, T., Sakamoto, S., Maeda, Y., Naruta, Y.
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Journal Title
Angew.Chem.Int.Ed. 48
Pages: 9262-9267
Related Report
Peer Reviewed
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[Journal Article] Geometric and electronic structure and reactivity of a mononuclear 'side-on' nickel(III)-peroxo complex2009
Author(s)
Cho, J., Sarangi, R, Annaraj, J., Kim, S.Y., Kubo, M., Ogura, T., Solomon, E.I., Nam, W.
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Journal Title
Nature Chemistry 1
Pages: 568-572
Related Report
Peer Reviewed
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[Journal Article] Oxidation Reactivity of a Bis(μ-oxo) Dinickel(III) Complexes : Arene Hydroxylation of the Supporting Ligand2009
Author(s)
Honda, K., Cho, J., Matsumoto, T., Roh, J., Furutachi, H., Tosha, T., Kubo, M., Fujinami, S., Ogura, T., Kitagawa, T., Suzuki, M.
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Journal Title
Angewandte Chemie, Int.Ed. 121
Pages: 3354-3357
Related Report
Peer Reviewed
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[Journal Article] Mononuclear Copper (II)-Superoxo Complexes that Mimic the Structure and Reactivity of the Active Centers of PHM and DβM2009
Author(s)
Kunishita, A., Kubo, M., Sugimoto, H., Ogura, T., Sato, K., Takui, T., Itoh, S.
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Journal Title
J.Am.Chem.Soc. 131
Pages: 2788-2789
Related Report
Peer Reviewed
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