Optical nano-instrumentation for observation of molecular dynamics

• Project/Area Number: 21360032
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied optics/Quantum optical engineering
• Research Institution: Osaka University
• Principal Investigator: INOUYE Yasushi 大阪大学, 生命機能研究科, 教授 (60294047)
• Co-Investigator(Renkei-kenkyūsha): SAKO Yasushi 理化学研究所, 基幹研究所, 主任研究員 (20215700) ; JIN Takashi 理化学研究所, 生命システム研究センター, チームリーダー (80206367)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: ナノ光計測 / 計測機器開発 / 金属ナノ粒子 / 生体分子カイネティクス / 1分子計測 / ナノプラズモニクス / 金属ナノクラスター / 転写制御因子 / SOX2 / 金ナノダイマー / プラズモニクス / FDTD / 半導体量子ドット / 表面修飾 / 生体イメージング

Research Abstract

We have developed optical nano-instrumentation for observation of biomolecular kinetics and molecular recognition mechanism by using novel optical nano-materials, such as metallic nano-particles, semiconductor quantum dots and metallic nano-clusters. We have also established fabrication methods of metallic nano-structures e. g., nano-dimers and nano-clusters which are suitable for observation of biomolecular dynamics besides optimization of nano-structures and development of chemical modifications.

Research Products

• [Journal Article] Fluorescent platinum nanoclusters : their synthesis, purification, characterization and application to bio-imaging (2011)
  • Author(s): S. Tanaka, J. Miyazaki, D. K. Tiwari, T. Jin, and Y. Inouye
  • Journal Title: Angew. Chem. Int. Ed Volume: 50 Pages: 431-435
  • Peer Reviewed
• [Journal Article] Single molecule detection from a large-scale SERS-active Au_79Ag_21 substrate (2011)
  • Author(s): H. Liu, L. Zhang, X. Lang, Y. Yamaguchi, H. Iwasaki, Y. Inouye, Q. Xue, and M. Chen
  • Journal Title: Sci. Rep Volume: 1 Pages: 112-112
  • Peer Reviewed
• [Journal Article] Mechanism of variable structural colour in the neon tetra : quantitative evaluation of the Venetian blind model (2011)
  • Author(s): S. Yoshioka, B. Matsuhana, S. Tanaka, Y. Inouye, N. Oshima, and S. Kinoshita
  • Journal Title: J. R. Soc. Interface Volume: 8 Pages: 56-66
  • Peer Reviewed
• [Journal Article] Single molecule detection from a large-scale SERS-active Au_<79>Ag_<21> substrate (2011)
  • Author(s): Hongwen Liu, Ling Zhang, Xingyou Lang, Yoshinori Yamaguchi, Hiroshi Iwasaki, Yasushi Inouye, 他2名
  • Journal Title: Scientific Reports Volume: 1 Issue: 1 Pages: 1121-5
  • DOI: 10.1038/srep00112
  • Peer Reviewed
• [Journal Article] Fluorescent Platinum Nanoclusters : their synthesis, purification, characterization and application to bio-imaging (2011)
  • Author(s): S.Tanaka, J.Miyazaki, D.K.Tiwari, T.Jin, Y.Inouye
  • Journal Title: Angew.Chem. Volume: 123 Pages: 451-455
  • Peer Reviewed
• [Journal Article] Mechanism of variable structural colour in the neon tetra : quantitative evaluation of the Venetian blind model (2011)
  • Author(s): S.Yoshioka, B.Matsuhana, S.Tanaka, Y.Inouye, 他2名
  • Journal Title: J.R.Soc.Interface Volume: 8 Pages: 56-66
  • Peer Reviewed
• [Journal Article] Antibody-ProteinA conjugated quantum dots for multiplexed imaging of surface receptors in living cells (2010)
  • Author(s): T. Jin, D. K. Tiwari, S. Tanaka, Y. Inouye, K. Yoshizawa, T. M. Watanabe
  • Journal Title: Molecular BioSystems Volume: 6 Pages: 2325-2331
  • Peer Reviewed
• [Journal Article] Synthesis and characterization of anti-HER2 antibody conjugated CdSe/ CdZnS quantum dots for fluorescence imaging of breast cancer cells (2009)
  • Author(s): D. K. Tiwari, S. Tanaka, Y. Inouye, K. Yoshizawa, T. M. Watanabe, and T. Jin
  • Journal Title: Sensors Volume: 9 Pages: 9332-9354
  • Peer Reviewed
• [Journal Article] Plasmonics for near-field nano-imaging and superlensing (2009)
  • Author(s): S. Kawata, Y. Inouye, and P. Verma
  • Journal Title: Nature Photonics Volume: 3 Pages: 388-394
  • Peer Reviewed
• [Journal Article] Synthesis and characterization of anti-HER2 antibody conjugated CdSe/CdZnS quantum dots for fluorescence imaging of breast cancer cells (2009)
  • Author(s): D.K.Tiwari, S.Tanaka, 他4名
  • Journal Title: Sensors 9 Pages: 9332-9354
  • Peer Reviewed
• [Journal Article] Plamonics for near-field nano-imaging and superlensing (2009)
  • Author(s): S.Kawata, Y.Inouye, P.Verma
  • Journal Title: Nature Photonics 3 Pages: 388-394
  • Peer Reviewed
• [Presentation] 金ナノダイマーによる生体分子のダイナミクス計測 (2012)
  • Author(s): 森村皓之、田中慎戸、石飛秀和、三上智之、蒲地雄介、近藤寿人、井上康志
  • Organizer: 第59回応用物理学関係連合講演会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-16
• [Presentation] 金ナノダイマーによる生体分子のダイナミクス計測 (2012)
  • Author(s): 森村皓之
  • Organizer: 2012年春季第59回応用物理学関係連合講演会
  • Place of Presentation: 早稲田大学早稲田キャンパス(東京都)
  • Year and Date: 2012-03-16
• [Presentation] Fluorescing platinum nanocluster and its application to bio-imaging (2012)
  • Author(s): Y. Inouye
  • Organizer: International Symposium on Nano Photonics 2012
  • Place of Presentation: Beijing, China
  • Year and Date: 2012-02-13
• [Presentation] Nano-observation of biomolecular dynamics using Au nanoparticle dimers (2012)
  • Author(s): H. Morimura, S. Tanaka, H. Ishitobi, T. Mikami, Y. Kamachi, H. Kondoh, and Y. Inouye
  • Organizer: International Symposium on Nano Photonics 2012
  • Place of Presentation: Beijing, China
  • Year and Date: 2012-02-13
• [Presentation] Fluorescing Platinum Nanocluster and Its Application to Bio-imaging (2012)
  • Author(s): 井上康志
  • Organizer: International Symposium on Nano Photonics 2012
  • Place of Presentation: Beijing Friendship Hotel(中国)(招待講演)
  • Year and Date: 2012-02-13
• [Presentation] 新規金属ナノプローブの開発と生命機能計測への展開 (2012)
  • Author(s): 田中慎一
  • Organizer: 大阪大学グローバルCOEプログラム「高次生命機能システムのダイナミクス」シンポジウム
  • Place of Presentation: 大阪大学銀杏会館(大阪府)
  • Year and Date: 2012-01-31
• [Presentation] ナノ金属による高感度・高分解センシング/イメージング (2011)
  • Author(s): 井上康志
  • Organizer: 静岡大学工学部プロジェクト第6回CREST研究会
  • Place of Presentation: 静岡大学工学部(静岡県)(招待講演)
  • Year and Date: 2011-09-06
• [Presentation] 蛍光性プラチナ・ナノクラスターの合成 (2011)
  • Author(s): 井上康志、田中慎戸、神隆
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形
  • Year and Date: 2011-08-31
• [Presentation] A Highly Fluorescent Platinum Nanocluster as a Bioimaging Probe (2011)
  • Author(s): S. Tanaka, J. Miyazaki, D. K. Tiwari, T. Jin, and Y. Inouye
  • Organizer: Molecular Plasmonics 2011
  • Place of Presentation: Jena, Germany
  • Year and Date: 2011-05-21
• [Presentation] Characterization of Fluorescence Properties of a Blue Emitting Au Nanocluster (2010)
  • Author(s): S. Tanaka, H. Morimura, D. K. Tiwari, J. Miyazaki, T. Jin, and Y. Inouye
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2010-12-18
• [Presentation] Characterization of Fluorescence Properties of a Blue Emitting Au Nanocluster (2010)
  • Author(s): 田中慎一
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii (USA)
  • Year and Date: 2010-12-18
• [Presentation] プラズモニクス技術の分光学的基礎とセンサー・ナノバイオへの応用の可能性 (2010)
  • Author(s): 井上康志
  • Organizer: オプトロニクス特別セミナー
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2010-11-25
• [Presentation] プラズモニクス技術の分光学的基礎とセンサー・ナノバイオへの応用の可能性 (2010)
  • Author(s): 井上康志
  • Organizer: 月刊OPTRONICS特別セミナー
  • Place of Presentation: 中央大学駿河台記念館(東京都)(招待講演)
  • Year and Date: 2010-11-25
• [Presentation] 蛍光性金ナノクラスターの開発及び生細胞観察への応用 (2010)
  • Author(s): 田中慎三、宮崎淳、D. K. Tiwari、神隆、井上康志
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-22
• [Presentation] 蛍光性金ナノクラスターの開発及び生細胞観察への応用 (2010)
  • Author(s): 田中慎一
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内キャンパス(宮城県)
  • Year and Date: 2010-09-22
• [Presentation] 近接場光学顕微分光法 (2010)
  • Author(s): 井上康志
  • Organizer: 日本分光学会第46回夏期セミナー
  • Place of Presentation: 千葉(招待講演)
  • Year and Date: 2010-09-01
• [Presentation] 近接場光学顕微分光法 (2010)
  • Author(s): 井上康志
  • Organizer: 日本分光学会第46回夏期セミナー
  • Place of Presentation: 幕張メッセ国際会議場(千葉県)(招待講演)
  • Year and Date: 2010-09-01
• [Presentation] "ナノバイオフォトニクス"フォトニクス技術フォーラム (2009)
  • Author(s): 井上康志
  • Organizer: 光情報技術研究会合同研究会
  • Place of Presentation: 大阪(招待講演)
  • Year and Date: 2009-11-30
• [Presentation] 蛍光性金属ナノクラスターの合成と蛍光イメージングへの展開 (2009)
  • Author(s): 井上康志、田中慎一、D. K. Tiwari、神隆
  • Organizer: 第70回応用物理学会学術講演会
  • Place of Presentation: 富山
  • Year and Date: 2009-09-09
• [Presentation] 蛍光性金属ナノクラスターの合成と蛍光イメージングへの展開 (2009)
  • Author(s): 井上康志, 他3名
  • Organizer: 第70回応用物理学会学術講演会
  • Place of Presentation: 富山大学
  • Year and Date: 2009-09-09
• [Presentation] 近接場光学顕微鏡による分光測定 (2009)
  • Author(s): 井上康志
  • Organizer: 日本分光学会第45回夏期セミナー
  • Place of Presentation: 千葉(招待講演)
  • Year and Date: 2009-09-02
• [Book] Molecular Nano Dynamics, vol.1 : Spectroscopic Methods and Nanostructures (2009)
  • Author(s): Y.Inouye, 他2名(分担執筆)
  • Total Pages: 314
  • Publisher: Wiley-VCH Verlag GmbH & Co.KGaA
• [Remarks] ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/1abs/Inoue/hp/Home.htm1