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The inhibition of ice growth, appropriate for the energy-saving and safety improvement for foods and medical treatment, by using antifreeze protein

Research Project

Project/Area Number 21360097
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Thermal engineering
Research InstitutionKyoto Institute of Technology

Principal Investigator

HAGIWARA Yoshimichi  京都工芸繊維大学, 工芸科学研究科, 教授 (50144332)

Co-Investigator(Renkei-kenkyūsha) KAKUTA Naoto  九州大学, 工学研究院, 准教授 (70345437)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords省エネルギー / 蛋白質 / 結晶成長 / 生物物理 / ナノバイオ
Research Abstract

We carried out measurement of temperature field for the solution of antifreeze protein under unidirectional freezing using near-infrared light. Also, we estimated protein concentration with fluorescence microscopy. We found that noticeable heat flux exist in the direction perpendicular to the ice growth direction in the serrated interface. This heat flux was due to the high concentration regions of antifreeze protein. The interface temperature for the mixed solution of antifreeze protein and sodium chloride is lower than the sum of the interface temperature for antifreeze protein solution and the interface temperature for sodium chloride solution. We obtained the results of molecular dynamics analysis, which was consistent with the measured result.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (46 results)

All 2012 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (37 results) Remarks (4 results)

  • [Journal Article] Temperature distribution and local heat flux in the unidirectional freezing of antifreeze-protein solution, Int.2012

    • Author(s)
      Y. Hagiwara and D. Yamamoto
    • Journal Title

      J. Heat and Mass Transfer

      Volume: Vol.55, No.9-10 Pages: 2384-2393

    • URL

      http://www.sciencedirect.com/science/article/pii/S001793102000361.

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cooperative effect of ions and winter flounder antifreeze protein near the ice/water interface2012

    • Author(s)
      K. Hayakari and Y. Hagiwara
    • Journal Title

      Molecular Simulation

      Volume: Vol.38, No.1 Issue: 1 Pages: 26-37

    • DOI

      10.1080/08927022.2011.600759

    • URL

      http://www.tandfonline.com/doi/abs/10.1080/08927022.2011.600759

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Temperature distribution and local heat flux in the unidirectional freezing of antifreeze-protein solution2012

    • Author(s)
      Yoshimichi Hagiwara, Daichi Yamamoto
    • Journal Title

      International Journal of Heat and Mass Transfer

      Volume: Vol.55 Issue: 9-10 Pages: 2384-2393

    • DOI

      10.1016/j.ijheatmasstransfer.2012.01.024

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sakurai and R. Nakanishi, Temperature of the solution of winter flounder antifreeze protein near ice surfaces in a narrow space, J.2010

    • Author(s)
      Y. Hagiwara, R.
    • Journal Title

      Crystal Growth

      Volume: vol.312, No.2 Pages: 314-322

    • URL

      http://www.sciencedirect.com/science/article/pii/S00220248/312/2

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Temperature of the solution of winter flounder antifreeze protein near ice surfaces in a narrow space2010

    • Author(s)
      Y.Hagiwara, R.Sakurai, R.Nakanishi
    • Journal Title

      Journal of Crystal Growth vol.312

      Pages: 314-322

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 微細流路内を流れる氷スラリーへの不凍タンパク質添加の影響2012

    • Author(s)
      中川嘉章
    • Organizer
      第49回日本伝熱シンポジウム
    • Place of Presentation
      富山市
    • Year and Date
      2012-06-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微細流路内を流れる不凍タンパク質水溶液の凝固過程における氷-溶液界面近傍の温度場・速度場・濃度場計測2012

    • Author(s)
      大西洋輔
    • Organizer
      第49回日本伝熱シンポジウム
    • Place of Presentation
      富山市
    • Year and Date
      2012-06-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微小流路内水流の凝固過程における温度場・速度場計測2011

    • Author(s)
      大西洋輔, 萩原良道, 山本大智, 前田穣, 北川石英
    • Organizer
      日本機械学会熱工学コンファレンス2
    • Place of Presentation
      浜松市,静岡大学
    • Year and Date
      2011-10-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 特定波長の近赤外光の吸収を用いた氷・不凍タンパク質水溶液界面近傍の温度場計測2011

    • Author(s)
      萩原良道, 山本大智
    • Organizer
      日本機械学会熱工学コンファレンス
    • Place of Presentation
      浜松市,静岡大学
    • Year and Date
      2011-10-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 特定波長の近赤外光の吸収を用いた氷・不凍タンパク質水溶液界面近傍の温度場計測2011

    • Author(s)
      萩原良道
    • Organizer
      日本機械学会熱工学コンファレンス2011
    • Place of Presentation
      浜松市
    • Year and Date
      2011-10-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微小流路内水流の凝固過程における温度場・速度場計測2011

    • Author(s)
      大西洋輔
    • Organizer
      日本機械学会熱工学コンファレンス2011
    • Place of Presentation
      浜松市
    • Year and Date
      2011-10-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 二次元ダクト内の過冷却水における氷結晶成長に関する数値解析2011

    • Author(s)
      鶴ヶ崎辰也, 立田健人, 萩原良道, 高木知弘
    • Organizer
      日本機械学会熱工学コンファレンス
    • Place of Presentation
      浜松市,静岡大学
    • Year and Date
      2011-10-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 二次元ダクト内の過冷却水における氷結晶成長に関する数値解析2011

    • Author(s)
      鶴ケ崎辰也
    • Organizer
      日本機械学会熱工学コンファレンス2011
    • Place of Presentation
      浜松市
    • Year and Date
      2011-10-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] A mixed solution of saline and HPLC6 and its effects on the interface with ice in unidirectional freezing2011

    • Author(s)
      Y. Maeda, D. Yamamoto and Y. Hagiwara
    • Organizer
      1st International Ice-binding Conference
    • Place of Presentation
      カナダキングストン市,クィーンズ大学.
    • Year and Date
      2011-08-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Temperature and heat flux of HPLC6 solution near serrated ice surfaces in unidirectional freezing2011

    • Author(s)
      Y. Hagiwara and D. Yamamoto
    • Organizer
      1st International Ice-binding Conference
    • Place of Presentation
      カナダキングストン市,クィーンズ大学
    • Year and Date
      2011-08-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Effects of hydration due to sodium ions on the motion of winter flounder antifreeze protein near a pyramidal ice surface2011

    • Author(s)
      K. Hayakari, Y. Hagiwara and K. Kuroshima
    • Organizer
      1st International Ice-binding Conference
    • Place of Presentation
      カナダキングストン市,クィーンズ大学
    • Year and Date
      2011-08-05
    • Related Report
      2011 Final Research Report
  • [Presentation] A mixed solution of saline and HPLC6 and its effects on the interface with ice in unidirectional freezing2011

    • Author(s)
      Yutaka Maeda
    • Organizer
      1st International Ice-binding Conference
    • Place of Presentation
      Kingston, Canada
    • Year and Date
      2011-08-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Temperature and heat flux of HPLC6 solution near serrated ice surfaces in unidirectional freezing2011

    • Author(s)
      Yoshimichi Hagiwara
    • Organizer
      1st International Ice-binding Conference
    • Place of Presentation
      Kingston, Canada
    • Year and Date
      2011-08-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effects of hydration due to sodium ions on the motion of winter flounder antifreeze protein near a pyramidal ice surface2011

    • Author(s)
      Yoshimichi Hagiwara
    • Organizer
      1st International Ice-binding Conference
    • Place of Presentation
      Kingston, Canada
    • Year and Date
      2011-08-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微小領域での水溶液の一方向凍結における不凍タンパク質と塩化ナトリウムの協同効果2011

    • Author(s)
      前田穣, 山本大智, 萩原良道
    • Organizer
      第48回日本伝熱シンポジウム
    • Place of Presentation
      岡山市,岡山コンベンションセンター
    • Year and Date
      2011-06-03
    • Related Report
      2011 Final Research Report
  • [Presentation] 微小領域での水溶液の-方向凍結における不凍タンパク質と塩化ナトリウムの協同効果2011

    • Author(s)
      前田穣
    • Organizer
      第48回日本伝熱シンポジウム
    • Place of Presentation
      岡山市
    • Year and Date
      2011-06-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 氷界面近傍の冬ガレイ由来不凍タンパク質水溶液中のタンパク質の局所濃度2011

    • Author(s)
      山本大智, 櫻井亮, 萩原良道, 北川石英
    • Organizer
      第48回日本伝熱シンポジウム
    • Place of Presentation
      岡山市,岡山コンベンションセンター
    • Year and Date
      2011-06-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 氷界面近傍の冬ガレイ由来不凍タンパク質水溶液中のタンパク質の局所濃度2011

    • Author(s)
      萩原良道
    • Organizer
      第48回日本伝熱シンポジウム
    • Place of Presentation
      岡山市
    • Year and Date
      2011-06-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 水・氷・混合体における水分子への力学的振動が及ぼす影響に関する分子動力学的研究2011

    • Author(s)
      黒島考平, 萩原良道, 早狩浩平
    • Organizer
      第48回日本伝熱シンポジウム
    • Place of Presentation
      岡山市,岡山コンベンションセンター
    • Year and Date
      2011-06-01
    • Related Report
      2011 Final Research Report
  • [Presentation] 水・氷・混合体における水分子への力学的振動が及ぼす影響に関する分子動力学的研究2011

    • Author(s)
      黒島考平
    • Organizer
      第48回日本伝熱シンポジウム
    • Place of Presentation
      岡山市
    • Year and Date
      2011-06-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effects of local concentration on freezing solutions of winter flounder antifreeze protein2011

    • Author(s)
      Y.Hagiwara
    • Organizer
      8th ASME/JSME Thermal Engineering Joint Conference
    • Place of Presentation
      米国ハワイ州ホノルル市
    • Year and Date
      2011-03-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 氷近傍の水中におけるイオンと不凍化タンパク質の相互作用2010

    • Author(s)
      萩原良道, 早狩浩平
    • Organizer
      第24回分子シミュレーション討論会
    • Place of Presentation
      福井市福井県県民ホール
    • Year and Date
      2010-11-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 氷近傍の水中におけるイオンと不凍化タンパク質の相互作用2010

    • Author(s)
      萩原良道
    • Organizer
      第24回分子シミュレーション討論会
    • Place of Presentation
      福井市
    • Year and Date
      2010-11-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 微小空間における不凍化タンパク質水溶液の氷界面近傍における局所温度および熱流束2010

    • Author(s)
      山本大智, 大西洋輔, 萩原良道
    • Organizer
      日本機械学会熱工学コンファレンス
    • Place of Presentation
      長岡市長岡科学技術大学
    • Year and Date
      2010-10-31
    • Related Report
      2011 Final Research Report
  • [Presentation] 微小空間における不凍化タンパク質水溶液の氷界面近傍における局所温度および熱流束2010

    • Author(s)
      山本大智
    • Organizer
      (社)日本機械学会熱工学コンファレンス
    • Place of Presentation
      長岡市
    • Year and Date
      2010-10-31
    • Related Report
      2010 Annual Research Report
  • [Presentation] マイクロチャネル内水流における氷結晶の成長への不凍タンパク質の影響2010

    • Author(s)
      大西洋輔, 山本大智, 萩原良道
    • Place of Presentation
      日本機械学会熱工学コンファレンス
    • Year and Date
      2010-10-30
    • Related Report
      2011 Final Research Report
  • [Presentation] マイクロチャネル内水流における氷結晶の成長への不凍タンパク質の影響2010

    • Author(s)
      大西洋輔
    • Organizer
      (社)日本機械学会熱工学コンファレンス
    • Place of Presentation
      長岡市
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cooperative effect of winter flounder antifreeze protein and ions on the unidirectional freezing of their solution in a narrow space2010

    • Author(s)
      Y. Hagiwara and D. Yamamoto
    • Organizer
      Physics and Chemistry of Ice
    • Place of Presentation
      札幌市,北海道大学
    • Year and Date
      2010-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] Cooperative effect of winter flounder antifreeze protein and ions on the unidirectional freezing of their solution in a narrow space2010

    • Author(s)
      Yoshimichi Hagiwara
    • Organizer
      12th Int.Conference on the Physics and Chemistry of Ice
    • Place of Presentation
      札幌市
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Local temperature and heat flux in the aqueous solution of antifreeze protein near ice surfaces in a narrow space2010

    • Author(s)
      Y. Hagiwara, D. Yamamoto and Y. Ohnishi
    • Organizer
      14th Int. Heat Transfer Conference
    • Place of Presentation
      米国ワシントンDC,オムニショアハムホテル
    • Year and Date
      2010-08-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Inhibition of blockage by ice crystal in water in a micro-channel by using antifreeze protein Proc2010

    • Author(s)
      Y. Hagiwara, Y. Ohnishi and D. Yamamoto
    • Organizer
      14th Int. Heat Transfer Conference
    • Place of Presentation
      米国ワシントンDC,オムニショアハムホテル
    • Year and Date
      2010-08-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Molecular dynamics analysis on the interaction among water, cations, anions and antifreeze protein2010

    • Author(s)
      K. Hayakari, Y. Kanamori and Y. Hagiwara
    • Organizer
      14th Int. Heat Transfer Conference
    • Place of Presentation
      米国ワシントンDC,オムニショアハムホテル.
    • Year and Date
      2010-08-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Local temperature and heat flux in the aqueous solution of antifreeze protein near ice surfaces in a narrow space2010

    • Author(s)
      Daichi Yamamoto
    • Organizer
      14th Int.Heat Transfer Conference
    • Place of Presentation
      米国ワシントンDC
    • Year and Date
      2010-08-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Inhibition of blockage by ice crystal in water in a micro-channel by using antifreeze protein2010

    • Author(s)
      Yosuke Ohnishi
    • Organizer
      14th Int.Heat Transfer Conference
    • Place of Presentation
      米国ワシントンDC
    • Year and Date
      2010-08-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular dynamics analysis on the interaction among water, cations, anions and antifreeze protein2010

    • Author(s)
      Yoshimichi Hagiwara
    • Organizer
      14th Int.Heat Transfer Conference
    • Place of Presentation
      米国ワシントンDC
    • Year and Date
      2010-08-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 不凍化タンパク質モデルあるいはイオンモデルと水分子との相互作用に関する分子動力学解析2009

    • Author(s)
      早狩浩平, 丸山善也, 萩原良道
    • Organizer
      第23回分子シミュレーション討論会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-12-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Effects of local concentration on freezing solutions of winter flounder antifreeze protein, Proc

    • Author(s)
      Y. Hagiwara, R. Sakurai, D. Yamamoto and A. Kitagawa
    • Organizer
      8th ASME/JSME Thermal Engineering Joint Conference
    • Place of Presentation
      米国ハワイ州ホノルル市,ワイキキビーチマリオットホテル
    • Related Report
      2011 Final Research Report
  • [Remarks] 特別講演Y. Hagiwara, Experimental and computational studies on the cooperative effects of ions and antifreeze protein, 2010年8月6日,米国ロサンジェルス市カリフォルニア州立大学ロサンジェルス校.

    • URL

      http://www.cis.kit.ac.jp/~kitagawa/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.cis.kit.ac.jp/~kitagawa/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.cis.kit.ac.jp/~kitagawa/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.cis-kit.ac.jp/~kitagawa/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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