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Functionalization of microbes cultured in 2D on the newly developed optical feedback system

Research Project

Project/Area Number 21360192
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field System engineering
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

OZASA Kazunari  独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (10231234)

Research Collaborator SIMON Song  Hanyang University, Korea, Professor
JEESOO Lee  Hanyang University, Korea, Doctor course student
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywords光フィードバック / ミドリムシ / マイクロ流路 / 光走性 / 化学走性 / ニューロコンピューティング / 微生物 / ユーグレナ / 揺らぎ / ニューラルコンピューティング / モンテカルロシミュレーション
Research Abstract

We developed a new optical feedback system for the photoreactive microbes confined in a microfluidic device. By using the photophobic reaction of Euglena, we succeeded in the control of cell density in the micro-aquarium dynamically with arbitrary pattern. We also achieved neuro-computing by introducing neural network algorithm in the feedback algorithm, by which optimized solution can be obtained with dynamic transition. Further, we succeeded in artificial connection between two individual culture system with optical feedback as well as chemical sensing using chemotaxis of the microbes.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (41 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (25 results) Remarks (2 results)

  • [Journal Article] Microfluidic Gas Sensing with Living Microbial Cells Confined in A Microaquarium2013

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Journal Title

      Key Eng. Mater

      Volume: 543 Pages: 431-434

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Euglena-based neurocomputing with two-dimensional optical feedback on swimming cells in micro-aquariums2013

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Journal Title

      Appl.Soft Comput

      Volume: 13 Pages: 527-538

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Euglena-based neurocomputing with two-dimensional optical feedback on swimming cells in micro-aquariums2013

    • Author(s)
      K.Ozasa
    • Journal Title

      Appl.Soft Comput.

      Volume: 13 Issue: 1 Pages: 527-538

    • DOI

      10.1016/j.asoc.2012.09.008

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microfluidic Gas Sensing with Living Microbial Cells Confined in A Microaquarium2013

    • Author(s)
      K.Ozasa
    • Journal Title

      Key Eng. Mater.

      Volume: 543 Pages: 431-434

    • DOI

      10.4028/www.scientific.net/kem.543.431

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surface-potential undulation of Alq3 thin films prepared on ITO, Au, and n-Si2012

    • Author(s)
      K.Ozasa
    • Journal Title

      J.Nanosci.Nanotechnol.

      Volume: 12 Issue: 1 Pages: 499-502

    • DOI

      10.1166/jnn.2012.5413

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Implementation of microbe-based neurocomputing with Euglena cells confined in microaquariums2011

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Journal Title

      Int.J.Unconventional Computing

      Volume: 7 Pages: 481-499

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two-dimensional optical feedback control of Euglena confined in closed-type microfluidic channels2011

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Journal Title

      LabChip

      Volume: 11 Pages: 1933-1940

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two-dimensional optical feedback control of Euglena confined in closed-type microfluidic channels2011

    • Author(s)
      Kazunari Ozasa
    • Journal Title

      Lab on a Chip

      Volume: 11 Pages: 1933-1940

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced evolution of surface-potential undulation observed on vacuum-deposited thin films of tris(8-hydroxyquinolinato) aluminum2011

    • Author(s)
      Kazunari Ozasa
    • Journal Title

      Appl.Phys.Lett.

      Volume: 98 Pages: 13301-13301

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simulation of neurocomputing based on the photophobic reactions of Euglena with optical feedback stimulation2010

    • Author(s)
      K.Ozasa, M.Aono, M.Maeda, M.Hara
    • Journal Title

      Biosystems

      Volume: 100 Pages: 101-107

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simulation of Neurocomputing Based on Photophobic Reactions of Euglena - Toward Microbe-Based Neural Network Computing -2009

    • Author(s)
      K.Ozasa, M.Aono, M.Maeda, M.Hara
    • Journal Title

      Lecture Notes in Computer Science

      Volume: 5715 Pages: 209-218

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simulation of Neurocomputing Based on Photophobic Reactions of Euglena-Toward Microbe-Based Neural Network Computing-2009

    • Author(s)
      Kazunari Ozasa
    • Journal Title

      Lecture Notes in Computer Science 5715

      Pages: 209-218

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Implementation of microbe-based neurocomputing with Euglena cells confined in microaquariums

    • Author(s)
      Kazunari Ozasa
    • Journal Title

      Int.J.Unconventional Computing

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simulation of neurocomputing based on the photophobic reactions of Euglena with optical feedback stimulation

    • Author(s)
      Kazunari Ozasa
    • Journal Title

      Biosystems (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effects of chemical diffusion through PDMS microchannels to living microbial cells2013

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      International Conference on Biomaterials Science 2013 (ICBS2013)
    • Place of Presentation
      Tsukuba, Japan
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Effects of Analog Feedback in Euglena-based Neural Network Computing- Experimental Research toward Network Optimization -2012

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      Korea-Japan joint workshop on complex communication sciences
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] Microfluidic Gas/Liquid Toxicity Sensing Through The Chemotaxis of Euglena Cells Confined in A Micro-Aquarium2012

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS2012)
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Optical Analog Feedback in Euglena-Based Neural Network Computing2012

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Maeda, M.Hara
    • Organizer
      Unconventional Computation and Natural Computation 2012 (UCNC2012)
    • Place of Presentation
      Orleans, France
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Chemotactic Toxicity Detection Using Euglena Cells Confined in An Isolated Micro-Aquarium2012

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      The Sixth International Conference on Quantum, Nano and Micro Technologies (ICQNM2012)
    • Place of Presentation
      Rome,Italy
    • Related Report
      2012 Final Research Report
  • [Presentation] Transient Characteristics in Photophobic Reactions of Euglena gracilis2012

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      American Society for Microbiology 112th General Meeting (ASM2012)
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Microfluidic Gas Sensing with Living Microbial Cells Confined in A Micro-Aquarium2012

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      International Conference on Materials and Applications for Sensors and Transducers (IC-MAST2012)
    • Place of Presentation
      Budapest, Hungary
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Chemotactic Toxicity Detection Using Euglena Cells Confined in An Isolated Micro-Aquarium2012

    • Author(s)
      K.Ozasa
    • Organizer
      The Sixth International Conference on Quantum, Nano and Micro Technologies (ICQNM2012)
    • Place of Presentation
      Rome, Italy
    • Related Report
      2012 Annual Research Report
  • [Presentation] マイクロ流路内のユーグレナに対するアナログ光フィードバック2011

    • Author(s)
      尾笹一成
    • Organizer
      日本物理学会2011年春季大会
    • Place of Presentation
      新潟
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Optical feedback control/analysis of photoreactive Euglena cells swimming in micro-aquariums2011

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS2011)
    • Place of Presentation
      Seatle, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Optical feedback control of Euglena cells in closed-type microfluidic channels2011

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      The Fifth International Conference on Quantum, Nano and Micro Technologies (ICQNM2011)
    • Place of Presentation
      Nice, France
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Possibility of incorporation of biomaterials in a hybrid device for two-dimensional optical feedback control2011

    • Author(s)
      K.Ozasa, M.Hara, M.Maeda
    • Organizer
      13th International Conference on Formation of Semiconductor Interfaces (ICFSI13)
    • Place of Presentation
      Prague, Czech Republic
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Analog feedback in Euglena-based neurocomputing2011

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      10th International Conference on Unconventional Computation (UC2011)
    • Place of Presentation
      Turku, Finland
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Optical feedback control of Euglena swimming in micro-aquarium2011

    • Author(s)
      J.Lee,S.Song,K.Ozasa,M.Hara,M.Maeda
    • Organizer
      The International Symposium on Microchemistry and Microsystems 2011 (ISMM2011)
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Optical feedback control of swimming Euglena in microaquariums2011

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Hara, M.Maeda
    • Organizer
      International Conference on Biomaterials Science 2011 (ICBS2011)
    • Place of Presentation
      Tsukuba, Japan
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] Optical feedback control/analysis of photoreactive Euglena cells swimming in micro-aquariums2011

    • Author(s)
      Kazunari Ozasa
    • Organizer
      15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2011)
    • Place of Presentation
      Seatle, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] マイクロ流路中のユーグレナへの光フィードバック2010

    • Author(s)
      尾笹一成
    • Organizer
      ユーグレナ研究会第26回研究集会
    • Place of Presentation
      東京
    • Year and Date
      2010-11-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Toward PostNANO technology-Optical control of photoresponsive microbes confined in microfluidic structures-2010

    • Author(s)
      Kazunari Ozasa
    • Organizer
      Asian Conference on Nanoscience and Nanotechnology 2010 (AsiaNANO2010)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2010-11-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] ユーグレナの光反応を用いたニューラルネットワークフィードバックシステムの動作2010

    • Author(s)
      尾笹一成
    • Organizer
      日本物理学会2010年秋季大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Implementation of microbe-based neurocomputing with living Euglena2010

    • Author(s)
      Kazunari Ozasa
    • Organizer
      9th International Conference on Unconventional Computation (UC10)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2010-06-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Toward PostNANO technology - Optical control of photoresponsive microbes confined in microfluidic structures -2010

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Maeda, M.Hara
    • Organizer
      Asian Conference on Nanoscience and Nanotechnology 2010 (AsiaNANO2010)
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Implementation of microbe-based neurocomputing with living Euglena2010

    • Author(s)
      K.Ozasa, J.Lee, S.Song, M.Maeda, M.Hara
    • Organizer
      9th International Conference on Unconventional Computation (UC10), WS-C
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] ユーグレナの光反応を用いたニューラルネットワークのシミュレーション2009

    • Author(s)
      尾笹一成
    • Organizer
      日本物理学会2009年秋季大会
    • Place of Presentation
      熊本市
    • Year and Date
      2009-09-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Simulation of Neurocomputing Based on Photophobic Reactions of Euglena-Toward Microbe-Based Neural Network Computing-2009

    • Author(s)
      尾笹一成
    • Organizer
      8th International Conference on Unconventional Computation(UC2009)
    • Place of Presentation
      Ponta Delgada, Portugal
    • Year and Date
      2009-09-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] Simulation of Neurocomputing Based on Photophobic Reactions of Euglena - Toward Microbe-Based Neural Network Computing -2009

    • Author(s)
      K.Ozasa, M.Aono, M.Maeda, M.Hara
    • Organizer
      8th International Conference on Unconventional Computation (UC2009)
    • Place of Presentation
      Ponta Delgada, Portugal
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.k-ozasa.net/

    • Related Report
      2012 Final Research Report
  • [Remarks] Profile and Research Kazunari Ozasa

    • URL

      http://www.k-ozasa.net/research_a.html

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2019-07-29  

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