| Project/Area Number |
21360199
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Measurement engineering
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| Research Institution | Kyushu University |
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Principal Investigator |
ENPUKU Keiji 九州大学, 超伝導システム科学研究センター, 教授 (20150493)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Masayoshi 九州大学, システム情報科学研究院, 准教授 (80346824)
KUMA Hiroyuki 長崎国際大学, 薬学部, 講師 (40435136)
YOSHISA Takashi 九州大学, システム情報科学研究院, 助教 (30380588)
NOZAKI Yukio 九州大学, システム情報科学研究院, 助教 (30304760)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 免疫検査 / 磁気マーカー / SQUIDセンサ / 磁気ナノ粒子 / ブラウン運動 / バイオ検査機器 / 液相検査 / ブラウン緩和 / SQUID / 磁気センサ |
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| Research Abstract |
We have developed a liquid-phase immunoassay technique using Brownian relaxation of magnetic markers. First, we quantitatively characterized the properties of the magnetic markers in order to find the markers suitable for the application. Next, we developed a detection method using large polymer beads to fix the biological targets, where bound and free markers were magnetically distinguished using the difference in their Brownian relaxation. Therefore, we can omit a time consuming washing process called bound/free separation. We also developed a detection system using high Tc SQUID sensor, and demonstrated the high sensitivity detection of biological targets called biotins.
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