Development of high performance quinone profiling method and application to environmental engineering.

• Project/Area Number: 21360252
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: Toyohashi University of Technology
• Principal Investigator: DAIMON Hiroyuki 豊橋技術科学大学, 大学院・工学研究科, 准教授 (60335106)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: キノンプロファイル / 微生物群集構造 / 超臨界流体抽出 / バイオマーカー / メタン発酵 / 土壌分析 / 液体肥料 / リン脂質脂肪酸 / 微生物群集構造解析 / 嫌気性処理 / 液状肥料 / リン脂質脂肪酸プロファイル

Research Abstract

The optimum conditions of extraction and determination in high performance quinone profiling method were investigated to improve the simplicity, speed and reliability on the procedure. This study showed the potential application of supercritical fluid extraction as a routine method for the analysis of microbial community structures in environmental assessment using the lipid biomarker profiles. Furthermore, application of the developed method was demonstrated to various environmental samples(anaerobic digestion, livestock excreta and composting process).

Research Products

• [Journal Article] Supercritical Fluid Extraction and Ultra Performance Liquid Chromatography of Respiratory Quinones for Microbial Community Analysis in Environmental and Biological Samples (2012)
  • Author(s): Muhammad Hanif, Yoichi Atsuta, Koichi Fujie and Hiroyuki Daimon
  • Journal Title: Molecules Volume: 17 Pages: 2628-2642
  • Peer Reviewed
• [Journal Article] Supercritical Fluid Extraction of Bacteria land Archaeal Lipid Biomarkers from An aerobically Digested Sludge (2012)
  • Author(s): Muhammad Hanif, Yoichi Atsuta, Koichi Fujie and Hiroyuki Daimon
  • Journal Title: International Journal of Molecular Science Volume: 13 Pages: 3022-3037
  • Peer Reviewed
• [Journal Article] Supercritical Fluid Extraction of Bacterialand Archaeal Lipid Biomarkers from Anaerobically Digested Sludge (2012)
  • Author(s): Muhammad Hanif, Yoichi Atsuta, Koichi Fujie, Hiroyuki Daimon
  • Journal Title: International Journal of Molecular Science Volume: 13 Issue: 3 Pages: 3022-3037
  • DOI: 10.3390/ijms13033022
  • Peer Reviewed
• [Journal Article] Supercritical Fluid Extraction of Microbial Phospholipid Fatty Acids from Activated Sludge (2010)
  • Author(s): Muhammad Hanif, Yoichi Atsuta, Koichi Fujie and Hiroyuki Daimon
  • Journal Title: Journal of Chromatography A Volume: 1217(43) Pages: 6704-6708
  • Peer Reviewed
• [Journal Article] Supercritical fluid extraction of microbial phospholipid fatty acids from activated sludge (2010)
  • Author(s): Muhammad Hanif, Youichi Atsuta, Koichi Fujie, Hiroyuki Daimon
  • Journal Title: Journal of Chromatography A Volume: 1217 Pages: 6704-6708
  • Peer Reviewed
• [Presentation] Monitoring of Microbial Community in Methane Fermentation Process of Palm Oil Mill Effluent using Lipid Biomarkers (2012)
  • Author(s): Sidik Marsudi, Muhammad Hanif, Yoichi Atsuta and Hiroyuki Daimon
  • Organizer: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
  • Place of Presentation: Orange County Convention Center, Florida, USA
• [Presentation] Supercritical Fluid Extraction of Lipid Biomarkers for Monitoring Microbial Community Structure in an Anaerobic Digestion (2012)
  • Author(s): Muhammad Hanif, Takeshi Yamada, Yoichi Atsuta and Hiroyuki Daimon
  • Organizer: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
  • Place of Presentation: Orange County Convention Center, Florida, USA
• [Presentation] Simultaneous Supercritical Fluid Extraction andChemical Derivatization of Microbial Phospholipids Fatty Acids from Anaerobically Digested Sludge (2012)
  • Author(s): Muhammad Hanif, Jin Yoshino, Yoichi Atsuta and Hiroyuki Daimon
  • Organizer: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
  • Place of Presentation: Orange County Convention Center, Florida, USA
• [Presentation] An Innovative Strategy for the Characterization of Microbial Lipid Biomarkers in the Environment using Supercritical Fluid Extraction (2012)
  • Author(s): Muhammad Hanif, Keita Ito, Yoichi Atsuta and Hiroyuki Daimon
  • Organizer: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
  • Place of Presentation: Orange County Convention Center, Florida, USA
• [Presentation] Simultaneous Supercritical Fluid Extraction and Chemical Derivatization of Microbial Phospholipids Fatty Acids from Anaerobically Digested Sludge (2012)
  • Author(s): Muhammad Hanif, Jin Yoshino, Yoichi Atsuta, Hiroyuki Daimon
  • Organizer: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
  • Place of Presentation: Orange County Convention Center, Florida, USA
• [Presentation] 嫌気性消化処理の前処理として用いる高温高圧水反応による下水混合汚泥の性状変化 (2012)
  • Author(s): 伊藤啓太, 蒲原弘継, 熱田洋一, 大門裕之
  • Organizer: 化学工学会第77年会
  • Place of Presentation: 工学院大学(東京都)
• [Presentation] 化学分析に基づいた微生物群集構造解析手法による嫌気性消化槽のモニタリング (2011)
  • Author(s): 大野祥平, Muhammad Hanif, Asri Gani, 熱田洋一, 大門裕之
  • Organizer: 日本水環境学会第45回年会
  • Place of Presentation: 北海道大学札幌キャンパス(北海道)
  • Year and Date: 2011-03-18
• [Presentation] Rapid Analysis of Bacterial and Archaeal Lipid Biomarkers during Methane Fermentation Process by Supercritical Fluid Extraction (2011)
  • Author(s): Muhammad Hanif, Yoichi Atsuta and Hiroyuki Daimon
  • Organizer: International Symposium on EcoTopia Science(ISETS)
  • Place of Presentation: Nagoya University, Aichi
• [Presentation] Application of Supercritical Fluid Technology for a Rapid Analysis of Microbial Lipid Biomarkers in Methane Fermentation Process (2011)
  • Author(s): Muhammad Hanif, Yoichi Atsuta and Hiroyuki Daimon
  • Organizer: The Asia Pacific Interdisciplinary Research Conference 2011
  • Place of Presentation: Toyohashi University of Technology, Aichi
• [Presentation] 化学分析に基づいた微生物群集構造解析手法による嫌気性消化槽のモニタリング (2011)
  • Author(s): 大野祥平, Muhammad Hanif, Asri Gani,熱田洋一,大門裕之
  • Organizer: 日本水環境学会第45回年会
  • Place of Presentation: 北海道大学(北海道)
• [Presentation] Application of Supercritical Fluid Technology for a Rapid Analysis of Microbial Lipid Biomarkers in Methane Fermentation Process (2011)
  • Author(s): Muhammad Hanif, Yoichi Atsuta, Hiroyuki Daimon
  • Organizer: The Asia-Pacific Interdisciplinary Research Conference 2011
  • Place of Presentation: Toyohashi University of Technology, Aichi, Japan
• [Presentation] Rapid Analysis of Bacterial and Archaeal Lipid Biomarkers during Methane Fermentation Process by Supercritical Fluid Extraction (2011)
  • Author(s): Muhammad Hanif, Yoichi Atsuta, Hiroyuki Daimon
  • Organizer: International Symposium on EcoTopia Science (ISETS)
  • Place of Presentation: Nagoya University, Aichi, Japan
• [Presentation] 超臨界流体技術を用いたキノンプロファイル法による嫌気性消化処理の安定性解析 (2010)
  • Author(s): 大野祥平, Muhammad Hanif, Ahamd Fall, 熱田洋一, 太門裕之
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 同志社大学今出川キャンパス(京都府)
  • Year and Date: 2010-09-07
• [Presentation] ハイパフォーマンスキノンプロファイル法を用いたメタン発酵槽内の微生物群集構造解析 (2010)
  • Author(s): 大門裕之
  • Organizer: (社)日本水環境学会中部支部講演会,(社)日本水環境学会
  • Place of Presentation: 愛知工業大学(愛知県)
  • Year and Date: 2010-08-11
• [Presentation] 超臨界二酸化炭素抽出法を用いたキノンプロファイル法の迅速・高精度化 (2010)
  • Author(s): 阪本崇
  • Organizer: 第44回日本水環境学会年会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2010-03-17
• [Presentation] パームオイル製油工場排水の嫌気性消化処理における有機物分解率の向上 (2010)
  • Author(s): 大野祥平
  • Organizer: 第44回日本水環境学会年会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2010-03-17
• [Presentation] 人工ゼオライトによるメタン発酵消化液の脱窒および窒素含有人工ゼオライトを用いた幼植物試験 (2010)
  • Author(s): 内田あゆ美,武田圭,鈴木邦彦,熱田洋一,大門裕之
  • Organizer: 第41回中化連秋季大会
  • Place of Presentation: 豊橋技術科学大学(愛知県)
• [Presentation] 超臨界二酸化炭素抽出法を用いたキノンプロファイル法によるメタン発酵槽中の微生物群集構造解析 (2010)
  • Author(s): 大野祥平, Muhammad Hanif, Ahamd Fall,熱田洋一,大門裕
  • Organizer: 第41回中化連秋季大会
  • Place of Presentation: 豊橋技術科学大学(愛知県)
• [Presentation] 超臨界流体技術を用いたキノンプロファイル法による嫌気性消化処理の安定性解析 (2010)
  • Author(s): 大野祥平, Muhammad Hanif, Ahamd Fall,熱田洋一,大門裕之
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 同志社大学(京都府)
• [Presentation] Application of Supercritical Fluid Extraction and Ultra Performance Liquid Chromatography to Microbial Quinone Analysis in Anaerobic Digestion (2010)
  • Author(s): Muhammad Hanif, Syouhei Ohno, Ahmed Fall, Yoichi Atsuta and Hiroyuki Daimon
  • Organizer: 34th International Symposium on Capillary Chromatography and 7th GCxGC Symposium
  • Place of Presentation: Congress Centre, Riva del Garda, Italy
• [Presentation] 超臨界二酸化炭素抽出法を用いたキノンプロファイル法の迅速・高精度化 (2010)
  • Author(s): 阪本嵩,小野孝之,熱田洋一,大門裕之
  • Organizer: 日本水環境学会第44回年会
  • Place of Presentation: 福岡大学(福岡県)
• [Presentation] パームいる製油工場排水の嫌気性消化処理における有機物分解率の向上 (2010)
  • Author(s): 大野祥平,熱田洋一,富内芳昌,大門裕之
  • Organizer: 日本水環境学会第44回年会
  • Place of Presentation: 福岡大学(福岡県)
• [Presentation] Rapid Identification of Microbial Quinones and Phospholipid Fatty Acids with Supercritical Fluid Extraction for Environmental Assessment (2010)
  • Author(s): Muhammad Hanif, Yoichi Atsuta, Takashi Sakamoto, Syouhei Ohno and Hiroyuki Daimon
  • Organizer: International Symposium on Hyphenated Techniques for Sample Preparation(HTSP)
  • Place of Presentation: Bruges, Belgium
• [Presentation] Rapid Identification of Microbial Quinones and Phospholipid Fatty Acids wity Supercritical Fluid Extraction for Environmental Assessment (2010)
  • Author(s): Muhammad Hanif
  • Organizer: International Symposium on Hyphenated Techniques for Sample Preparation
  • Place of Presentation: Bruges, Belgium
• [Presentation] SFE-UPLCによるキノンプロファイル法の迅速化 (2009)
  • Author(s): 大野祥平
  • Organizer: 化学工学会 第41回秋季大会
  • Place of Presentation: 広島県東広島市
  • Year and Date: 2009-09-16
• [Presentation] Characterization of Microbial Communities in Anaerobic Digestion Process using Quinone Profiles (2009)
  • Author(s): Muhammad Hanif, Yoichi Atsuta and Hiroyuki Daimon
  • Organizer: 1st International Conference on Engineering, Environment, Economics, Safety & Health
  • Place of Presentation: Manado, Indonesia
• [Presentation] SFE-UPLCによるキノンプロファイルの迅速化 (2009)
  • Author(s): 大野祥平,熱田洋一,大門裕之
  • Organizer: 化学工学会第41回秋季大会
  • Place of Presentation: 広島大学(広島県)
• [Presentation] Characterization of Microbial Communities in Anaerobic Digestion Process using Quinone Profiles (2009)
  • Author(s): Muhammad Hanif
  • Organizer: International Conference on Engineering, Environment, Economic, Safety & Health
  • Place of Presentation: Manado, Indonesia