Study on utilization technology of oxygen-tolerant denitrifying microorganisms in sewage and wastewater treatment to prevent global warming
Project/Area Number |
21360258
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Civil and environmental engineering
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Research Institution | Tohoku Gakuin University |
Principal Investigator |
ENDO Ginro 東北学院大学, 工学部, 教授 (80194033)
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Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Kanji 東北学院大学, 工学部, 教授 (90382655)
MIYAUCHI Keisuke 東北学院大学, 工学部, 教授 (20324014)
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Project Period (FY) |
2009 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
|
Keywords | 地球温暖化ガス排出削減 / 硝化脱窒 / 温室効果ガス / 亜酸化窒素(N_2O) / 耐酸素性脱窒細菌 / 分子生物学的解析 / 亜酸化窒素(N2O) / 亜酸化窒素 / 耐酸素性脱窒素細菌 / 温室効果ガス削減 / 定量PCR(リアルタイムPCR) / nosZ遺伝子 / 亜硝酸還元 / 増殖特性 / 集積培養 / 16SrRNA遺伝子 / nosZ遺伝 |
Research Abstract |
In this research project, we developed an effective method to monitor the behavior of nitrous oxide reductase gene (nosZ) in nitrification and denitrification processes by using quantitative PCR (realtime PCR) method. After the development, Pseudomonas stutzeri TR2 that was known as an oxygen tolerant bacterium was introduced into nitrification and denitrification systems, and its survival and denitrification activity were investigated. New denitrifying bacteria were isolated and selected under aerobic and nitrite-existing conditions from activated sludge obtained from a wastewater treatment plant. Among the 20 bacterial isolates, some of them were exhibited high denitrification capability with no emission of nitrous oxide under existing dissolved oxygen. These isolates also show high growth capability with nitrous oxide as a sole source of electron acceptor. Therefore, these isolates originated from activated sludge were considered to be applicable to the technology for reduction of nitrous oxide emission.
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Report
(6 results)
Research Products
(55 results)
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[Journal Article] Survival of the Aerobic Denitrifier <I>Pseudomonas stutzeri</I> Strain TR2 during Co-Culture with Activated Sludge under Denitrifying Conditions2012
Author(s)
M. Miyahara, S.-W. Kim, S. Zhou, S.Fushinobu, T. Yamada, W. Ikeda-Ohtsubo, A. Watanabe, K. Miyauchi, G. Endo, T. Wakagi, and H. Shoun
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76
Issue: 3
Pages: 495-500
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Aerobic denitrification by Pseudomonas stutzeri TR2 has the potential to reduce nitrous oxide emission from wastewater treatment plants2010
Author(s)
M.Miyahara, S-W.Kim, S.Fushinobu, K.Takaki, T.Yamada, A.Watanabe, K.Miyauchi, G.Endo, T.Wakagi, H.Shoun
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Journal Title
Applied and Environmental Microbiology
Volume: Vol.76, No.14
Pages: 4619-4625
Related Report
Peer Reviewed
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[Presentation] Pilot scale bioaugmentation for removing nitrous oxide (N2O) using denitrifying bacterium Pseudomonas stutzeri strain TR2; a case study, Environmental Microbiology and Biotechnology 20122012
Author(s)
W. Ikeda-ohtsubo, M. Miyahara, K. Sang-Wan, A. Watanabe, S. Fushinobu, T. Wakagi, H. Shoun, K. Miyauchi, G. Endo
Organizer
Environmental Microbiology and Biotechnology 2012
Place of Presentation
School of Engineering, Alma Mater Studiorum, University of Bologna, Bologna
Year and Date
2012-04-11
Related Report
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[Presentation] Pilot scale bioaugmentation for removing nitrous oxide (N_2O) using denitrifying bacterium Pseudomonas stutzeri strain TR2 ; a case study2012
Author(s)
W. Ikeda-ohtsubo, M. Miya hara, K. Sang-Wan, A. Watanabe, S. Fushinobu, T. Wakagi, H. Shoun, K. Miyauchi, G. Endo
Organizer
Environmental Microbiology and biotechnology 2012
Place of Presentation
School of Engineering, Alma Mater Studiorum, University of Bologna, Bologna, Italy
Year and Date
2012-04-11
Related Report
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[Presentation] Pilot scale bioaugmentation for removing nitrous oxide (N2O) using denitrifying bacterium Pseudomonas stutzeri strain TR2; a case study2012
Author(s)
W. Ikeda-ohtsubo, M. Miyahara, K. Sang-Wan, A. Watanabe, S. Fushinobu, T. Wakagi, H. Shoun, K. Miyauchi, G. Endo
Organizer
Environmental Microbiology and Biotechnology 2012
Place of Presentation
School of Engineering, Alma Mater Studiorum, University of Bologna, Bologna, Italy
Related Report
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