| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Research Abstract |
In this research project, we developed an effective method to monitor the behavior of nitrous oxide reductase gene (nosZ) in nitrification and denitrification processes by using quantitative PCR (realtime PCR) method. After the development, Pseudomonas stutzeri TR2 that was known as an oxygen tolerant bacterium was introduced into nitrification and denitrification systems, and its survival and denitrification activity were investigated. New denitrifying bacteria were isolated and selected under aerobic and nitrite-existing conditions from activated sludge obtained from a wastewater treatment plant. Among the 20 bacterial isolates, some of them were exhibited high denitrification capability with no emission of nitrous oxide under existing dissolved oxygen. These isolates also show high growth capability with nitrous oxide as a sole source of electron acceptor. Therefore, these isolates originated from activated sludge were considered to be applicable to the technology for reduction of nitrous oxide emission.
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