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Practical control of pathogens which cases stock animal diseases

Research Project

Project/Area Number 21360399
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo Institute of Technology

Principal Investigator

TANJI Yasunori  東京工業大学, 大学院・生命理工学研究科, 教授 (00282848)

Co-Investigator(Kenkyū-buntansha) MIYANAGA Kazuhiko  東京工業大学, 大学院・生命理工学研究科, 助教 (40323810)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsバクテリオファージ / 乳房炎 / 黄色ブドウ球菌 / Staphylococcus aureus / ファージセラピー / 耐性菌 / クテリオファージ / 黄色ブドウ球 / 下水流入水 / 多剤耐性菌 / 凝集性
Research Abstract

49 S. aureus isolates were obtained from the milk of mastitic cows for use in the screening of staphylococcal phages. The host ranges of phages isolated were determined, and two phages were subsequently chosen for analysis.ΦSA039 had the widest host range, producing clear plaques on 13 of the 15 isolates, while□ΦSA012 produced clear plaques on 8 isolates. This study constitutes a solid basis on which to continue the exploration of the potential ofΦSA012 andΦSA039 in the treatment of staphylococcal diseases.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (54 results)

All 2012 2011 2010 2009 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (33 results) Book (2 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Diffusion of bacteriophages through an artificial biofilm model. Biotechnol. Prog2012

    • Author(s)
      J. Hu, K. Miyanaga, Y. Tanji
    • Journal Title

      DOI

      Volume: 10 Pages: 742-742

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diffusion of bacteriophages through an artificial biofilm model2012

    • Author(s)
      J.Hu, K.Miyanaga, Y.Tanji
    • Journal Title

      Biotechnol.Prog.

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diffusion properties of bacteriophages through agarose gel membrane.2011

    • Author(s)
      J.Hu, K.Miyanaga, Y.Tanji
    • Journal Title

      Biotechnol. Prog.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diffusion properties of bacteriophages through agarose gel membrane. Biotechnol2010

    • Author(s)
      J. Hu, K. Miyanaga, Y. Tanji
    • Journal Title

      Prog

      Volume: 26(5) Pages: 1213-1221

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intercrossing of Phage Genomes in a Phage Cocktail and Stable Coexistence with Escherichia coli O157 : H7 in Anaerobic Continuous Culture Appl. Microbiol2010

    • Author(s)
      H. Kunisaki, Y. Tanji
    • Journal Title

      Biotechnol

      Volume: 85 Pages: 1533-1541

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intercrossing of Phage Genomes in a Phage Cocktail and Stable Coexistence with Escherichia coli O157:H7 in Anaerobic Continuous Culture2010

    • Author(s)
      H.Kunisaki, Y.Tanji.
    • Journal Title

      Appl.Microbiol. Biotechnol.

      Volume: 85 Pages: 1533-1541

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intercrossing of Phage Genomes in a Phage Cocktail and Stable Coexistence with Escherichia coli O157:H7 in Anaerobic Continuous Culture2010

    • Author(s)
      H.Kunisaki, Y.Tanji.
    • Journal Title

      Appl.Microbiol.Biotechnol., 85

      Pages: 1533-1541

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of milk on antibacterial activity of tetracycline against Escherichia coli and Staphylococcus aureus isolated from bovine mastitis. Appl. Microbiol2009

    • Author(s)
      Y. Kuang, H. Jia, K. Miyanaga, Y. Tanji
    • Journal Title

      Biotechnol

      Volume: 84 Pages: 135-142

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation from sewage influent and characterization of novel Staphylococcus aureus bacteriophages with wide host range and potent lytic capability. Apple. Env2009

    • Author(s)
      A. J. Synnott, Y. Kuang, M. Kurimoto, K. Yamamichi, H. Iwano.Y Tanji
    • Journal Title

      Microbiol

      Volume: 75 Pages: 4283-4490

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recombination of T2 bacteriophage using IP008 phage long tail fiber genes expands host range while retaining lytic activity. FEMS Microbiol. Letter, 295, 211-217(2009)(査読有) 7. Y. Kuang, K. Tani, A. J. Synnott, K. Ohshima, H. Higuchi, H. Nagahata, Y. Tanji2009

    • Author(s)
      F. Mahichi. A. J. Synnott, K. Yamamichi, T. Osada, Y. Tanji
    • Journal Title

      Biochem. Eng. J

      Volume: 295 Pages: 211-217

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR-DGGE method2009

    • Author(s)
      Y. Kuang, K. Tani, A. J. Synnott, K. Ohshima, H. Higuchi, H. Nagahata, Y. Tanji
    • Journal Title

      Biochem. Eng. J

      Volume: 45 Pages: 76-81

    • NAID

      120006582021

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] IgA response of BALB/c mice to orally administered Salmonella Typhimurium flagellin-displaying T2 bacteriophages2009

    • Author(s)
      A. Synnott, K. Ohshima, Y. Nakai, Y. Tanji
    • Journal Title

      Biotechnol. Prog

      Volume: 25 Pages: 552-558

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recombination of T2 bacteriophage using IP008 phage long tail fiber genes expands host range while retaining lytic activity2009

    • Author(s)
      F.Mahichi., A.J.Synnott, K.Yamamichi, T.Osada, Y.Tanji
    • Journal Title

      FEMS Microbiol.Letter 295

      Pages: 211-217

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation from sewage influent and characterization of novel Staphylococcus aureus bacteriophages with wide host range and potent lytic capability2009

    • Author(s)
      J.Synnott, Y.Kuang, M.Kurimoto, K.Yamamichi, H.Iwano.Y Tanji.
    • Journal Title

      Apple.Env.Microbiol, 75

      Pages: 4283-4490

    • NAID

      120006581987

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of milk on antibacterial activity of tetracycline against Escherichia coli and Staphylococcus aureus isolated from bovine mastitis2009

    • Author(s)
      Y.Kuang, H.Jia, K.Miyanaga, Y.Tanji.
    • Journal Title

      Appl.Microbiol.Biotechno., 84

      Pages: 135-142

    • NAID

      120006582017

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大腸菌O157:H7特異的ファージの宿主認識機構解析2012

    • Author(s)
      全且雄, 宮永一彦, 丹治保典
    • Organizer
      第14回化学工学会学生発表会
    • Place of Presentation
      東京高専
    • Year and Date
      2012-03-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] バクテリオファージに対する牛乳房炎起因黄色ブドウ球菌の耐性化機構の解析2012

    • Author(s)
      長田啓太, 田中愛里, 宮永一彦, 丹治保典
    • Organizer
      第14回化学工学会学生発表会
    • Place of Presentation
      東京高専
    • Year and Date
      2012-03-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] バクテリオファージによる病原菌の制御と検出」第16回2011

    • Author(s)
      丹治保典
    • Organizer
      日本乳房炎研究会学術集会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-10-14
    • Related Report
      2011 Final Research Report
  • [Presentation] バクテリオファージによる病原菌の制御と検出2011

    • Author(s)
      丹治保典
    • Organizer
      第16回日本乳房炎研究会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] ファージセラピーへ向けた牛乳房炎起因黄色ブドウ球菌のファージ被感染性評価2011

    • Author(s)
      田中愛里、長田啓太、宮永一彦、永幡肇、岩野英知、樋口豪紀、丹治保典
    • Organizer
      第16回日本乳房炎研究会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Diffusion properties of bacteriophages through a biofilm model, 19 th Biennial Evergreen International Phage Biology Meeting2011

    • Author(s)
      Hu Jun, Y. Tanji
    • Organizer
      The Evergreen State College
    • Place of Presentation
      lympia, WA, USA
    • Year and Date
      2011-09-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛乳房炎起因S2011

    • Author(s)
      田中愛里、長田啓太、宮永一彦、永幡肇、岩野英知、樋口豪紀、丹治保典
    • Organizer
      第43回化学工学秋季大会
    • Place of Presentation
      名古屋工業大学
    • Related Report
      2011 Final Research Report
  • [Presentation] Diffusion properties of bacteriophages through a biofilm model2011

    • Author(s)
      J.Hu, K.Miyanaga, Y.Tanji
    • Organizer
      The 19th International Phage Biology Meeting
    • Place of Presentation
      シアトル(USA)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 牛乳房炎起因黄色ブドウ球菌のファージと抗生物質の感受性評価2011

    • Author(s)
      田中愛里, 長田啓太, 宮永一彦, 永幡肇, 岩野英知, 樋口豪紀, 丹治保典
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 牛乳房炎ファージセラピーに向けたS. aureusの凝集性評価2010

    • Author(s)
      丹治保典、谷夏織、栗本実希、宮永一彦、樋口豪紀、岩野英知、井上博紀、永幡肇
    • Organizer
      第15回日本乳房炎研究会学術集会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2010-10-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛乳房炎ファージセラピーに向けた黄色ブドウ球菌の凝集性評価2010

    • Author(s)
      丹治保典
    • Organizer
      乳房炎研究会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2010-10-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 黄色ブドウ球菌に対する特異的なファージを用いたウシ乳房炎治療応用への可能性2010

    • Author(s)
      岩野英知、丹治保典
    • Organizer
      乳房炎研究会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2010-10-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] バクテリオファージによる細菌の検出とコントロール2010

    • Author(s)
      丹治保典
    • Organizer
      Bio Japan
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-09-29
    • Related Report
      2011 Final Research Report
  • [Presentation] バクテリオファージによる細菌の検出とコントロール2010

    • Author(s)
      丹治保典
    • Organizer
      バイオジャパン2010
    • Place of Presentation
      パシフィコ横浜展示会場
    • Year and Date
      2010-09-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Diffusion properties of bacteriophages through agarosegel membrane2010

    • Author(s)
      Hu Jun、丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2011 Final Research Report
  • [Presentation] T4系バクテリオファージの大腸菌O157認識に関わる解析2010

    • Author(s)
      淺川宏章、丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2011 Final Research Report
  • [Presentation] 大腸菌O157感染性ファージの宿主特異性に関する解析2010

    • Author(s)
      森川真年、丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛乳房炎由来Staphylococcus aureusとその特異的ファージの相互作用におけるイオン強度の影響2010

    • Author(s)
      栗本実希、丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛乳房炎ファージセラピーに向けた取り組み2010

    • Author(s)
      丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2011 Final Research Report
  • [Presentation] Potential Application of Bacteriophage for Controlling and Detection of Pathogen2010

    • Author(s)
      丹治保典
    • Organizer
      2010 International Symposium on Advanced Biological Engineering
    • Place of Presentation
      Beijing friendship hotel
    • Related Report
      2010 Annual Research Report
  • [Presentation] 牛乳房炎ファージセラピーに向けた取り組み2010

    • Author(s)
      丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー「バクテリオファージ研究の可能性と課題」
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2010 Annual Research Report
  • [Presentation] バクテリオファージ研究の可能性と課題2010

    • Author(s)
      森川真年、宮永一彦、丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー「バクテリオファージ研究の可能性と課題」
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2010 Annual Research Report
  • [Presentation] T4系バクテリオファージの大腸菌O157認識に関わる解析2010

    • Author(s)
      淺川宏章、宮永一彦、丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー「バクテリオファージ研究の可能性と課題」
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2010 Annual Research Report
  • [Presentation] 牛乳房炎由来Staphylococcus aureusとその特異的ファージの相互作用におけるイオン強度の影響2010

    • Author(s)
      栗本実希、宮永一彦、丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー「バクテリオファージ研究の可能性と課題」
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2010 Annual Research Report
  • [Presentation] Diffusion properties of bacteriophages through agarose gel membraneバクテリオファージのアガロースゲル内の拡散2010

    • Author(s)
      Hu Jun, 宮永一彦、丹治保典
    • Organizer
      大阪大学蛋白質研究所セミナー「バクテリオファージ研究の可能性と課題」
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2010 Annual Research Report
  • [Presentation] 牛乳房炎のファージセラピー実現に向けた試み2009

    • Author(s)
      丹治保典、宮永一彦、岩野英知
    • Organizer
      第14回日本乳房炎研究会学術集会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2009-10-23
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛乳房炎のファージセラピー実現に向けた試み2009

    • Author(s)
      丹治保典
    • Organizer
      第14回日本乳房研究会学術集会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] ファージセラピーは抗生物質治療に取って代われるか2009

    • Author(s)
      丹治保典
    • Organizer
      Bio Japan
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-10-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛乳房炎由来Staphylococcus aureusの凝集体形成の評価2009

    • Author(s)
      谷夏織、丹治保典
    • Organizer
      111回日本畜産学会
    • Place of Presentation
      琉球大学
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 牛乳房炎起因黄色ブドウ球菌に対するファージ及び抗生物質の感受性の評価2009

    • Author(s)
      栗本実希、丹治保典
    • Organizer
      111回日本畜産学会
    • Place of Presentation
      琉球大学
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 家畜感染症制を目指すファージセラピーの実践的試み2009

    • Author(s)
      丹治保典、Aidan Synnott
    • Organizer
      111回日本畜産学会
    • Place of Presentation
      琉球大学
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] wano, Control of Staphylococcus aureus which causes bovine mastitis by bacteriophages with wide host range and potent lytic capability, 18th Biennial Evergreen International Phage Biology Meeting2009

    • Author(s)
      Tanji, A. Synnott, Y. Kuang, M. Kurimoto, K. Yamamichi & H. Iwano
    • Organizer
      The Evergreen State College, Olympia
    • Place of Presentation
      WA, USA, Aug
    • Year and Date
      2009-09-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Two-chamber method to measure diffusion coefficient of bacteriophage in biofilm2009

    • Author(s)
      Hu Jun、宮永一彦、丹治保典
    • Organizer
      第41回化学工学秋季大会
    • Place of Presentation
      広島大学
    • Related Report
      2011 Final Research Report
  • [Book] ファージと病原菌の奇妙な関係、未来材料2010

    • Author(s)
      丹治保典
    • Related Report
      2011 Final Research Report
  • [Book] バクテリオファージによる大腸菌O157 : H7の同定、臨床検査2009

    • Author(s)
      丹治保典
    • Related Report
      2011 Final Research Report
  • [Remarks] 本課題研究に関する新聞掲載記事全酪新報、新しい乳房炎の治療法を模索(ファージで原因菌除去) 2011年11月10日

    • URL

      http://www.biochemeng.bio.titech.ac.jp/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.biochemeng.bio.titech.ac.jp/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.biochemeng.bio.titech.ac.jp/index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] スタフィロコッカス・アウレウス溶菌性バクテリオファージ2009

    • Inventor(s)
      丹治保典、宮永一彦、岩野英知、樋口豪紀、井上博紀、永幡肇、横田博、近藤昭宏、小倉俊樹
    • Filing Date
      2009-12-25
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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