Creation of stress tolerant strain based on single nucleotide mutational analysis of in whole genome
| Project/Area Number |
21360401
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
SHIMIZU Hiroshi 大阪大学, 大学院・情報科学研究科, 教授 (00226250)
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| Co-Investigator(Kenkyū-buntansha) |
FURUSAWA Chikara 大阪大学, 大学院・情報科学研究科, 准教授 (00372631)
HIRASAWA Takashi 大阪大学, 大学院・情報科学研究科, 助教 (20407125)
ONO Naoaki 大阪大学, 大学院・情報科学研究科, 特任准 (60395118)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | ゲノム / DNAマイクロアレイ / 変異解析 / ストレス耐性 / 進化工学 / バイオプロダクション / 発現プロファイル / 比増殖速度 |
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| Research Abstract |
We performed experimental evolution under(5%) ethanol stress condition in Escherichia coli and six ethanol stress tolerant evolved strains were independently obtained. Transcriptomic analysis of evolved strains was performed. As a result of principal component analysis(PCA), gene expression in biosynthesis pathways of some amino acids were extracted and these are commonly up-regulated in the tolerant strains, suggesting that activating these pathways were involved in the achievement of ethanol tolerance. In support of this hypothesis, the supplementation of these amino acids to the culture medium increased the specific growth rate under ethanol stress. Mutational analysis was also performed by high-density oligonucleotide microarray and it was suggested that mutation does not significantly contribute to ethanol stress tolerance because there few common mutations among evolved strains.
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Report
(4 results)
Research Products
(58 results)
