Delignification of cellulosic biomass by a bacterium

• Project/Area Number: 21360403
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kansai University (2010-2011); Osaka University (2009)
• Principal Investigator: KATAKURA Yoshio 関西大学, 化学生命工学部, 教授 (50263207)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: バイオ生産プロセス / リグノセルロース / Ureibacillus thermosphaericus / 生物的脱リグニン / バイオマス / Mn-peroxidase / caffeic acid / セルロース結合ドメイン / 糖化率 / Ureibacillus thermoshaericus / コーンコブ / guaiacol / pyrogallol / セルロース

Research Abstract

To establish biological delignification of biomass by Ureibacillus thermosphaericus, a rapid method evaluating the degree of deliginification using cellulose binding domain from Thermobifida fsuca Cel9B was developed. This bacterium was found to produce Mn-peroxidase and Cu-amine oxidase. It was possible to produce these enzymes by cultivating this bacterium in a synthetic medium containing seven amino acids and two vitamins.

Research Products

• [Presentation] 基質認識部位の除去がcellobiohydrolase Iの比活性に及ぼす影響 (2011)
  • Author(s): 片倉啓雄、橋爪祥輝、紀ノ岡正博
  • Organizer: セルラーゼ研究会
  • Place of Presentation: 花王土浦研修所
  • Year and Date: 2011-10-15
• [Presentation] 基質認識部位の除去がcellobiohydrolase Iの比活性に及ぼす影響 (2011)
  • Author(s): 片倉啓雄, 橋爪祥輝, 紀ノ岡正博
  • Organizer: 日本生物工学会平成23年度大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-09-26
• [Presentation] Ureibacillus thermosphaericusを用いたリグノセルロース系バイオマスの生物的脱リグニン (2010)
  • Author(s): 猪熊淳史, 福井純一, 関野宏紀, 片倉啓雄, 紀ノ岡正博
  • Organizer: 化学工学会関西支部第8回最先端バイオテクノロジー発表会
  • Place of Presentation: 大阪市立大学(大阪市)
  • Year and Date: 2010-02-17
• [Book] 前処理および糖化の評価とプロセスの最適化,セルロース系バイオエタノール製造技術 (2010)
  • Author(s): 片倉啓雄
• [Remarks]
  • URL: http://microbial.life-bio.kansai-u.ac.jp/
• [Remarks]
  • URL: http://microbial.life-bio.kansai-u.ac.jp/
• [Patent(Industrial Property Rights)] エタノールの製造方法 (2010)
  • Inventor(s): 片倉啓雄、野田秀夫
  • Industrial Property Rights Holder: 関西化学機械製作(株)、大阪大学
  • Filing Date: 2010-08-31