Analysis of affinity maturation process of antibodies using a novel FDC line and its application.
| Project/Area Number |
21360405
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Okayama University |
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Principal Investigator |
OMORI Hitoshi 岡山大学, 大学院・自然科学研究科, 教授 (70116440)
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| Co-Investigator(Renkei-kenkyūsha) |
MAGARI Masaki 岡山大学, 大学院・自然科学研究科, 助教
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | モノクローナル抗体 / リンパ球 / 突然変異 / アポトーシス / 生体防御 / 胚中心 / 抗体医薬 / 濾胞樹状細胞 / 抗体 / 体細胞高頻度突然変異 / 親和性成熟 / IL-21 / AID / B細胞株 |
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| Research Abstract |
Using a novel murine follicular dendritic cell line, FL-Y, I analyzed affinity maturation process of antibodies focusing on B cell selection in germinal centers(GCs). I found that FL-Y cells induced B cell death in the presence of a helper T cell cytokine, IL-21. The B cell death was shown to be dependent on PGE2 produced from FL-Y cells, The IL-21/PGE2-induced B cell death implicates a new negative selection mechanism of B cells in GCs.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of antibody repertoire.2010
Author(s)
Magari, M., Kanehiro, Y., Todo, K., Ikeda, M., Kanayama, N., Ohmori, H.
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Journal Title
Biochem.Biphys.Res.Commmun.
Volume: 396
Pages: 353-358
Related Report
Peer Reviewed
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[Journal Article] Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation2010
Author(s)
Kajita, M., Okazawa, T., Ikeda, M., Todo, K., Magari, M., Kanayama, N., Ohmori, H.
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Journal Title
J.Biosci.Bioeng.
Volume: 351
Pages: 351-358
NAID
Related Report
Peer Reviewed
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[Journal Article] Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression.2009
Author(s)
Magari, M., Aya, T., Ikeda, M., Todo, K.Kanayam, N., Ohmori, H.
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Journal Title
Journal of Bioscience and Bieengineering 107
Pages: 206-209
NAID
Related Report
Peer Reviewed
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[Book] 改訂細胞工学2010
Author(s)
永井和夫、大森斉、金山直樹、町田千代子
Total Pages
230
Publisher
講談社
Related Report
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[Book] 改訂 細胞工学2010
Author(s)
永井和男、大森齊、町田千代子、金山直樹
Total Pages
230
Publisher
講談社サイエンティフィック
Related Report
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