Development of a pyrosequence-based technique for HIV drug-resistance diagnosis
Project/Area Number |
21360408
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Waseda University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
SUGIURA Wataru (独)国立病院機構名古屋医療センター, 臨床研究センター感染免疫研究部, 部長 (70300936)
OKAMURA Yoshiko 広島大学, 先端物質科学研究科, 准教授 (80405513)
MORI Tetsushi 早稲田大学, 理工学術院, 助教 (00590100)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
|
Keywords | pyrosequencing / HIV / quasispecies / 同時並列個別増幅 / 一分子計測 / パイロシークエンス / ゲノム / 多様性解析 / シングルセル解析 |
Research Abstract |
The aim of this research is to develop a single-cell analytical technique based on pyrosequencing to accurately identify diverse mutations within HIV and to conduct sequence-based elucidation of the mechanisms related to HIV quasispecies formation. As a result, we were successful in the establishment of a system for measuring cDNA molecules to as low as 10 copies. Furthermore, we were also successful in tracing the coevolution between the subtypes of HIV quasispecies-related proviral DNA, Gag and PR.
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Report
(4 results)
Research Products
(28 results)