A comprehensive study of the molecular mechanisms of associative learning by a behavioral genetic approach.

• Project/Area Number: 21370002
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: IINO Yuichi 東京大学, 大学院・理学系研究科, 教授 (40192471)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 学習記憶 / 線虫C. elegans / 化学走性行動 / インスリン / PTEN / 左右非対称性 / PI3キナーゼ / カルシンテニン / 学習 / 忌避行動 / 嗅覚可塑性 / グアニル酸シクラーゼ / フルオリン / 感覚繊毛 / カプサイシン受容体

Research Abstract

The nematode C. elegans learns to avoid salt after the experience of starvation in the presence of salt. A pair of sensory neurons sense salt, but only the right member of this pair changes by learning through the effect of the C. elegans insulin. In addition, a protein known to be relevant to Alzheimer's desease was found closely related to the action of the insulin signal.

Research Products

• [Journal Article] Neuronal plasticity regulated by theinsulin-like signaling pathway underliessalt chemotaxis learning in Caenorhabditiselegans (2011)
  • Author(s): Oda, S., Tomioka, M., and Iino, Y
  • Journal Title: J Neurophysiol Volume: VOL. 106 Pages: 301-308
  • Peer Reviewed
• [Journal Article] Roles for class IIA phosphatidylinositol transfer protein in neurotransmission and behavioral plasticity at the sensory neuron synapses of Caenorhabditis elegans (2011)
  • Author(s): Iwata, R., Oda, S., Kunitomo, H., and Iino, Y
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Issue: 18 Pages: 7589-7594
  • DOI: 10.1073/pnas.1016232108
  • Peer Reviewed
• [Journal Article] Behavioral Choice between Conflicting Alternatives Is Regulated by a Receptor Guanylyl Cyclase, GCY-28, and a Receptor Tyrosine Kinase, SCD-2, in AIA Interneurons of Caenorhabditis elegans (2011)
  • Author(s): Shinkai, Y., Yamamoto, Y., Fujiwara, M., Tabata, T., Murayama, T., Hirotsu, T., Ikeda, D.D., Tsunozaki, M., Iino, Y., Bargmann, C.I., et al.
  • Journal Title: J Neurosci Volume: 31 Issue: 8 Pages: 3007-3015
  • DOI: 10.1523/jneurosci.4691-10.2011
  • Peer Reviewed
• [Journal Article] Neuronal plasticity regulated by the insulin-like signaling pathway underlies salt chemotaxis learning in Caenorhabditis elegans (2011)
  • Author(s): 小田茂和、富岡征大、飯野雄一
  • Journal Title: Journal of Neurophysiology Volume: 106 Issue: 1 Pages: 301-308
  • DOI: 10.1152/jn.01029.2010
  • Peer Reviewed
• [Journal Article] Insulin signaling plays a dual role in Caenorhabditis elegans memory acquisition and memory retrieval (2010)
  • Author(s): Lin, C.H., Tomioka, M., Pereira, S., Sellings, L., Iino, Y., and van der Kooy, D
  • Journal Title: J Neurosci Volume: 30 Issue: 23 Pages: 8001-8011
  • DOI: 10.1523/jneurosci.4636-09.2010
  • Peer Reviewed
• [Journal Article] Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs) (2010)
  • Author(s): Kimura, Y., Kurabe, N., Ikegami, K., Tsutsumi, K., Konishi, Y., Kaplan, O.I., Kunitomo, H., Iino, Y., Blacque, O.E., and Setou, M
  • Journal Title: J Biol Chem Volume: 285 Issue: 30 Pages: 22936-22941
  • DOI: 10.1074/jbc.c110.128280
  • Peer Reviewed
• [Journal Article] Olfactory plasticity is regulated by pheromonal signaling in Caenorhabditis elegans (2010)
  • Author(s): Yamada, K., Hirotsu, T., Matsuki, M., Butcher, R.A., Tomioka, M., Ishihara, T., Clardy, J., Kunitomo, H., and Iino, Y
  • Journal Title: Science Volume: 329 Issue: 5999 Pages: 1647-1650
  • DOI: 10.1126/science.1192020
  • Peer Reviewed
• [Journal Article] Reversal of Salt Preference Is Directed bythe Insulin/PI3K and Gq/PKC Signaling in Caenorhabditis elegans (2010)
  • Author(s): Adachi, T., Kunitomo, H., Tomioka, M., Ohno, H., Okochi, Y., Mori, I., and Iino, Y
  • Journal Title: Genetics Volume: 186 Pages: 1309-1319
  • Peer Reviewed
• [Journal Article] Single-cell transcriptional analysis of taste sensory neuron pair in Caenorhabditis elegans (2010)
  • Author(s): Takayama, J., Faumont, S., Kunitomo, H., Lockery, S. R., and Iino, Y
  • Journal Title: Nucleic Acids Research Volume: 38 Issue: 1 Pages: 131-142
  • DOI: 10.1093/nar/gkp868
  • Peer Reviewed
• [Journal Article] Insulin singnaling plays a dual role in Caenorhabditis elegans memory acquisition acquisition and memory retrieval (2010)
  • Author(s): Lin, C.H., 他
  • Journal Title: Journal of Neuroscience Volume: 30 Pages: 8001-80011
  • Peer Reviewed
• [Journal Article] Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs) (2010)
  • Author(s): Kimura, Y., 他
  • Journal Title: J Biol Chem Volume: 285 Pages: 22936-22941
  • Peer Reviewed
• [Journal Article] Olfactory plasticity is regulated by pheromonal signaling in Caenorhahditis elegans (2010)
  • Author(s): Yamada, K., 他
  • Journal Title: Science Volume: 329 Pages: 1647-1650
  • Peer Reviewed
• [Journal Article] Reversal of Salt Preference Is Directed by the Insulin/PI3K and Gq/PKC Signaling in Caenorhabditis elegans (2010)
  • Author(s): Adachi, T., 他
  • Journal Title: Genetics Volume: 186 Pages: 1309-1319
  • Peer Reviewed
• [Journal Article] Behavioral Choice between Conflicting Alternatives Is Regulated by a Receptor Guanylyl Cyclase, GCY-28, and a Receptor Tyrosine Kinase, SCD-2, in AIA Interneurons of Caenorhabditis elegans (2010)
  • Author(s): Shinkai, Y., 他
  • Journal Title: J Neurosci Volume: 31 Pages: 3007-3015
  • Peer Reviewed
• [Journal Article] Single-cell transcriptional analysis of taste sensory neuron pair in Caenorhabditis elegans. (2010)
  • Author(s): Takayama, J., 他
  • Journal Title: Nucleic Acids Research 38 Pages: 131-142
  • Peer Reviewed
• [Journal Article] Lateralized gustatory behavior of C. elegans is controlled by specific receptor-type guanylyl cyclases (2009)
  • Author(s): Ortiz, C.O., Faumont, S., Takayama, J., Ahmed, H.K., Goldsmith, A.D., Pocock, R., McCormick, K.E., Kunimoto, H., Iino, Y., Lockery, S., et al.
  • Journal Title: Curr Biol Volume: 19 Issue: 12 Pages: 996-1004
  • DOI: 10.1016/j.cub.2009.05.043
  • Peer Reviewed
• [Journal Article] A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans (2009)
  • Author(s): Hayashi, Y., Hirotsu, T., Iwata, R., Kage-Nakadai, E., Kunitomo, H., Ishihara, T., Iino, Y., and Kubo, T
  • Journal Title: Nat Neurosci Volume: 12 Issue: 8 Pages: 981-987
  • DOI: 10.1038/nn.2347
  • Peer Reviewed
• [Journal Article] Lateralized gustatory behavior of C.elegans is controlled by specific receptor-type guanylyl cyclases. (2009)
  • Author(s): Ortiz, C.O., 他
  • Journal Title: Current Biology 19 Pages: 996-1004
  • Peer Reviewed
• [Journal Article] A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans. (2009)
  • Author(s): Hayashi, Y., 他
  • Journal Title: Nature Neuroscience 12 Pages: 981-987
  • Peer Reviewed
• [Presentation] Dynamics of neural activities underlying salt chemotaxis learning in C. elegans (2011)
  • Author(s): 小田茂和
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] プロテインキナーゼCによる線虫の塩濃度学習の制御機構の解析 (2011)
  • Author(s): 田村成宏
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Analyses of molecular mechanisms of information ntegration in food-dependent salt chemotaxis learning in C. elegans (2011)
  • Author(s): 酒井奈緒子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Dynamics of neural activities underlying salt chemotaxis learning in C.elegans (2011)
  • Author(s): 小田茂和
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Analyses of molecular mechanisms of information ntegration in food-dependent salt chemotaxis learning in C.elegans (2011)
  • Author(s): 酒井奈緒子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] The role of insulin/PI3-kinase pathway in food-dependentplasticity of salt chemotaxis in C. elegans. (2011)
  • Author(s): 富岡征大
  • Organizer: Neuroscience 2011, SfN's 41st annual meeting
  • Place of Presentation: ワシントンDC
  • Year and Date: 2011-11-16
• [Presentation] The role of insulin/PI 3-kinase pathway in food-dependent plasticity of salt chemotaxis in C.elegans (2011)
  • Author(s): 富岡征大
  • Organizer: Neuroscience 2011, SfN's 41st annual meeting
  • Place of Presentation: ワシントンDC 米国
  • Year and Date: 2011-11-16
• [Presentation] Sensing Salt : Plasticity inSensory Systems and Behavioral Response (2011)
  • Author(s): 飯野雄一
  • Organizer: 18th international C. elegans meeting
  • Place of Presentation: ロサンゼルス
  • Year and Date: 2011-06-24
• [Presentation] Axonal transport of a new DAF-2 isoform governs associativelearning (2011)
  • Author(s): 大野速雄
  • Organizer: 18th international C. elegans meeting
  • Place of Presentation: ロサンゼルス
  • Year and Date: 2011-06-24
• [Presentation] Axonal transport of a new DAF-2 isoform governs associative learning (2011)
  • Author(s): 大野速雄
  • Organizer: 18th international C.elegans meeting
  • Place of Presentation: ロサンゼルス 米国
  • Year and Date: 2011-06-24
• [Presentation] Sensing Salt : Plasticity in Sensory Systems and Behavioral Response (2011)
  • Author(s): 飯野雄一
  • Organizer: 18th international C.elegans meeting
  • Place of Presentation: ロサンゼルス 米国(招待講演)
  • Year and Date: 2011-06-24
• [Presentation] Molecular pathways and neural circuit that underlie the plasticityof salt chemotaxis in C. elegans (2010)
  • Author(s): 飯野雄一
  • Organizer: BMB2010第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸・神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Molecular pathways and neural circuit that underlie the plasticity of salt chemotaxis in C.elegans (2010)
  • Author(s): 飯野雄一
  • Organizer: BMB2010第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸・神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] 線虫C. elegansの学習を制御するインスリン様シグナル伝達経路の役割 (2010)
  • Author(s): 富岡征大
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 東京・東京大学教養学部
  • Year and Date: 2010-09-24
• [Presentation] 線虫C.elegansの学習を制御するインスリン様シグナル伝達経路の役割 (2010)
  • Author(s): 富岡征大
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 東京・東京大学教養学部
  • Year and Date: 2010-09-24
• [Presentation] Pheromonal interactions modulate behavioral plasticity in C. elegans (2010)
  • Author(s): 飯野雄一
  • Organizer: 4th East Asia C. elegans Meeting
  • Place of Presentation: 東京・オリンピック記念青少年総合センター
  • Year and Date: 2010-07-14
• [Presentation] Pheromonal interactions modulate behavioral plasticity in C.elegans (2010)
  • Author(s): 飯野雄一
  • Organizer: 4th East Asia C.elegans Meeting
  • Place of Presentation: 東京・オリンピック記念青少年総合センター
  • Year and Date: 2010-07-14
• [Presentation] C. elegans has a memory for NaCl concentrations (2010)
  • Author(s): 大野速雄
  • Organizer: 4th East Asia C. elegans Meeting
  • Place of Presentation: 東京・オリンピック記念青少年総合センター
  • Year and Date: 2010-07-13
• [Presentation] Neural and behavioral mechanisms of response to an odorant depending on its concentration (2010)
  • Author(s): 吉田和史
  • Organizer: 4th East AsiaC. elegans Meeting
  • Place of Presentation: 東京・オリンピック記念青少年総合センター
  • Year and Date: 2010-07-13
• [Presentation] C.elegans has a memory for NaC1 concentrations (2010)
  • Author(s): 大野速雄
  • Organizer: 4th East Asia C.elegans Meeting
  • Place of Presentation: 東京・オリンピック記念青少年総合センター
  • Year and Date: 2010-07-13
• [Presentation] Neural and behavioral mechanisms of response to an odorant depending on its concentration (2010)
  • Author(s): 吉田和史
  • Organizer: 4th East Asia C.elegans Meeting
  • Place of Presentation: 東京・オリンピック記念青少年総合センター
  • Year and Date: 2010-07-13
• [Presentation] Synaptic regulation of DAG and phosphoinositides by M01F1. 7, the conserved phosphatidylinositol transfer protein (2010)
  • Author(s): 岩田遼
  • Organizer: 4th East Asia C. elegans Meeting
  • Place of Presentation: 東京・オリンピック記念青少年総合センター
  • Year and Date: 2010-07-12
• [Presentation] Synaptic regulation of DAG and phosphoinositides by M01F1.7, the cons erved phosphatidylinositol transfer protein (2010)
  • Author(s): 岩田遼
  • Organizer: 4th East Asia C.elegans Meeting
  • Place of Presentation: 東京・オリンピック記念青少年総合センター
  • Year and Date: 2010-07-12
• [Presentation] Ascaroside phromones regulate olfactory plasticity through the SNET-1 peptide., Neuronal Development, Synaptic Function & Behavior C (2010)
  • Author(s): 飯野雄一
  • Organizer: Neuronal Development, Synaptic Function & Behavior C. elegans Topic Meeting 2010
  • Place of Presentation: マディソン, U. S. A
  • Year and Date: 2010-06-29
• [Presentation] Neural and behavioral mechanisms of response to an odorant depending on its concentration. (2010)
  • Author(s): 吉田和史
  • Organizer: Neuronal Development, Synaptic Function & Behavior C. elegans Topic Meeting 2010
  • Place of Presentation: マディソン, U. S. A
  • Year and Date: 2010-06-29
• [Presentation] Dynamics of neural circuitunderlying salt chemotaxis learning in C. elegans. (2010)
  • Author(s): 小田茂和
  • Organizer: Neuronal Development, Synaptic Function & Behavior C. elegans Topic Meeting 2010
  • Place of Presentation: マディソン, U. S. A
  • Year and Date: 2010-06-29
• [Presentation] The role of INS-1, an insulin-like peptide, in salt chemotaxis learning. (2010)
  • Author(s): 富岡征大
  • Organizer: Neuronal Development, Synaptic Function & Behavior C. elegans Topic Meeting 2010
  • Place of Presentation: マディソン, U. S. A
  • Year and Date: 2010-06-29
• [Presentation] Neural and behavioral mechanisms of response to an odorant depending on its concentration (2010)
  • Author(s): 吉田和史
  • Organizer: Neuronal Development, Synaptic Function & Beh avior C.elegans Topic Meeting 2010
  • Place of Presentation: マディソン,U.S.A
  • Year and Date: 2010-06-29
• [Presentation] Dynamics of neural circuit underlying salt chemotaxis learning in C.elegans (2010)
  • Author(s): 小田茂和
  • Organizer: Neuronal Development, Synaptic Function & Behavior C.elegans Topic Meeting 2010
  • Place of Presentation: マディソン,U.S.A.
  • Year and Date: 2010-06-29
• [Presentation] The role of INS-1, an insulin-like peptide, in salt chemotaxis learning (2010)
  • Author(s): 富岡征大
  • Organizer: Neuronal Development, Synaptic Function & Beh avior C.elegans Topic Meeting 2010
  • Place of Presentation: マディソン,U,S.A.
  • Year and Date: 2010-06-29
• [Presentation] Ascaroside phromones regulate olfactory plasticity through the SNET-1 peptide (2010)
  • Author(s): 飯野雄一
  • Organizer: Neuronal Development, Synaptic Function & Beh avior C.elegans Topic Meeting 2010
  • Place of Presentation: マディソン,U.S.A.
  • Year and Date: 2010-06-29
• [Presentation] 線虫C. elegansの味覚神経ASERによる塩走性学習の制御とその分子メカニズム (2009)
  • Author(s): 安達健, 他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-13
• [Presentation] 線虫C.elegansの味覚神経ASERによる塩走性学習の制御とその分子メカニズム (2009)
  • Author(s): 安達健, 他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-13
• [Presentation] 線虫C. elegansの塩走性学習におけるインスリン様ペプチドINS-1の分泌の役割 (2009)
  • Author(s): 富岡征大他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] C.エレガンスの塩走性行動の制御機構の解明 (2009)
  • Author(s): 國友博文他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] 線虫C.elegansの塩走性学習におけるインスリン様ペプチドINS-1の分泌の役割 (2009)
  • Author(s): 富岡征大, 他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Strength of salt chemotaxis learning intensity and retention of sensory signaling of memor y i n Caenorhabditiselegans (2009)
  • Author(s): 小野寺馨他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Strength of salt chemotaxis learning intensity and retention of sensory signaling of memory in Caenorhabditis elegans (2009)
  • Author(s): 小野寺馨, 他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Dopamine-depende ntsecretion of INS-1, an insulin-likepeptide, regulates salt chemotaxislearning in C. elegans (2009)
  • Author(s): 富岡征大他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] Dopamine-dependent secretion of INS-1, an insulin-like peptide, regulates salt chemotaxis learning in C.elegans (2009)
  • Author(s): 富岡征大, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] A combination of salt and food conditions in the habitat affects preference for salt in C.elegans. (2009)
  • Author(s): 國友博文, 他
  • Organizer: 17th International C.elegans Meeting
  • Place of Presentation: ロサンジェルス(米国)
  • Year and Date: 2009-06-27
• [Remarks]
  • URL: http://molecular-ethology.biochem.s.u-tokyo.ac.jp/IINO_lab_J.html
• [Remarks]
  • URL: http://molecular-ethology.biochem.s.u-tokyo.ac.jp/IINO_lab_J.html
• [Remarks]
  • URL: http://molecular-ethology.biochem.s.u-tokyo.ac.jp/IINO_lab_J.html