Molecular Genetics of Sleep in Drosophila

• Project/Area Number: 21370003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyushu University
• Principal Investigator: TANIMURA Teiichi 九州大学, 大学院・理学研究院, 准教授 (20142010)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: ショウジョウバエ / 睡眠 / アデノシン受容体 / 行動解析 / サーカディアンリズム / ウルトラディアンリズム / 時計突然変異体 / ショウジョウバェ

Research Abstract

Sleep research is important for people in modern world as we are faced with many problems associated with good sleep and sleep disorders. Recent studies indicate that Drosophila can be used as an excellent model organism to study on sleep. We have developed an CCD camera-PC system to track movement of a single fly. We found that Drosophila have an endogenous ultradian oscillator, which is masked by circadian rhythmic behaviors. The pdf clock mutant shows robust and precise ultradian rhythm with period length about 55 minutes. The ultradian rhythm of pdf mutant flies was not temperature compensated. We also studied the neural mechanism of sleep regulation by caffeine to reveal the possible role of the adenosine receptor.

Research Products

• [Journal Article] Taste preference for amino acids is dependent on internal nutritional state in Drosophila (2012)
  • Author(s): Toshima, N. and Tanimura, T.
  • Journal Title: J. Exp. Biol. in press
  • Peer Reviewed
• [Journal Article] Drosophila evaluates and learns the nutritional value of sugars (2011)
  • Author(s): Fujita, M. and Tanimura, T.
  • Journal Title: Curr. Biol Volume: 21 Pages: 751-755
  • Peer Reviewed
• [Journal Article] bHLH-ORANGE family genes regulate the expression of E-box clock genes in Drosophila (2011)
  • Author(s): Itoh, T. Q., Tanimura, T. and Matsumoto, A
  • Journal Title: Appl. Entomol. Zool Volume: 46 Pages: 391-397
  • NAID: 40018958900
  • Peer Reviewed
• [Journal Article] A membrane-bound transporter controls the circadian transcription of clock genes in Drosophila (2011)
  • Author(s): Itoh, T. Q., Tanimura, T. and Matsumoto, A.
  • Journal Title: Genes to Cells Volume: 16 Pages: 1159-1167
  • Peer Reviewed
• [Journal Article] Identification of a novel gene, Anorexia, regulating feeding activity via insulin signaling in Drosophila melanogaster (2011)
  • Author(s): Ryuda, M. Tsuzuki, S., Matsumoto, H., Oda, O., Tanimura T. and Hayakawa, Y.
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 38417-38426
  • Peer Reviewed
• [Journal Article] Yoshikawa H. A gustatory receptor involved in host-plant recognition for oviposition of the butterfly (2011)
  • Author(s): Ozaki K, Ryuda M, Yamada A, Utoguchi A, Ishimoto H, Calas D, Marion-Poll F, Tanimura T.
  • Journal Title: Papilio xuthus. Nat. commun Volume: 2 Pages: 542-542
  • Peer Reviewed
• [Journal Article] bHLH-ORANGE family genes regulate the expression of E-box clock genes in Drosophila (2011)
  • Author(s): Itoh, et al
  • Journal Title: Applied Entomology and Zoology Volume: 46 Issue: 3 Pages: 391-397
  • DOI: 10.1007/s13355-011-0052-z
  • Peer Reviewed
• [Journal Article] Identification of a novel gene, Anorexia, regulating feeding activity via insulin signaling in Drosophila melanogaster (2011)
  • Author(s): Ryuda, M., et al
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 44 Pages: 38417-38426
  • DOI: 10.1074/jbc.m111.267344
  • Peer Reviewed
• [Journal Article] Drosophila evaluates and learns the nutritional value of sugars. (2011)
  • Author(s): Fujita, M., Tanimura, T.
  • Journal Title: Current Biology Volume: (in press)
  • Peer Reviewed
• [Journal Article] ハエは夢をみるのか? (2010)
  • Author(s): 関裕一
  • Journal Title: 昆虫と自然 Volume: 45 Pages: 4-7
  • Peer Reviewed
• [Journal Article] ハエは夢をみるのか? (2010)
  • Author(s): 関裕一
  • Journal Title: 昆虫と自然 45 Pages: 4-7
• [Journal Article] The clock gene cryptochrome of Bactrocera cucurbitae(Diptera : Tephritidae) in strains with different mating times (2009)
  • Author(s): Fuchikawa, T., Sanada, S., Nishio, R., Matsumoto, A., Matsuyama, T., Yamagishi, M., Tomioka, K., Tanimura, T. and Miyatake T.
  • Journal Title: Heredity Volume: 104 Pages: 387-392
  • Peer Reviewed
• [Journal Article] The clock gene cryptochrome of Bactrocera cucurbitae(Diptera : Tephritieae)in strains with different mating times. (2009)
  • Author(s): Fuchikawa, T., et al.
  • Journal Title: Heredity 104 Pages: 387-392
  • Peer Reviewed
• [Presentation] E23 controls Drosophila circadian rhythm to modulate the clock gene vrille expression through molting steroid hormone response (2011)
  • Author(s): 伊藤太
  • Organizer: Cold Spring Harbor Meeting
  • Place of Presentation: アメリカ合衆国
  • Year and Date: 2011-10-03
• [Presentation] 時計遺伝子clockwork orangeはホモダイマーで機能する (2010)
  • Author(s): 伊藤太一, 谷村禎一, 松本顕
  • Organizer: 日本分子生物学会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] ナミアゲハ(Papilio xuthus)ふ節感覚子の産卵刺激物質応答に関する電気生理学的解析 (2010)
  • Author(s): 龍田勝輔, 谷村禎一, 尾崎克久
  • Organizer: 日本味と匂学会第44回大会
  • Place of Presentation: 福岡県北九州市
  • Year and Date: 2010-09-09
• [Presentation] ショウジョウバエにおける苦味受容の行動生理学的解析 (2010)
  • Author(s): 新里直, 張暁〓, 谷村禎一
  • Organizer: 日本味と匂学会第44回大会
  • Place of Presentation: 福岡県北九州
  • Year and Date: 2010-09-09
• [Presentation] Nutritional value learning in Drosophila. (2010)
  • Author(s): Tanimura, T.
  • Organizer: Nutritional Homeostasis Workshop
  • Place of Presentation: ボン、ドイツ共和国
  • Year and Date: 2010-05-03
• [Presentation] Ultradian rhythm is revealed by disruption of circadian clock in Drosophila melanogaster (2010)
  • Author(s): Yuuichi Seki and Teiichi Tanimura
  • Organizer: ESF-EMBO Conference on Functional Neurobiology in Minibrains From Flies to Robots and Back Again
  • Place of Presentation: Sant Feliu de Guixols, Spain
• [Presentation] キイロショウジョウバエpdf[ 01]突然変異体におけるウルトラディアンリズム (2009)
  • Author(s): 関裕一、谷村禎一
  • Organizer: 第30回に日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-14