Molecular and Experimental Ecology in Hemerocallis : As a good model to study evolutionary shift of Pollination syndromes

• Project/Area Number: 21370013
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: Kyushu University
• Principal Investigator: YAHARA Tetsukazu 九州大学, 大学院・理学研究院, 教授 (90158048)
• Co-Investigator(Kenkyū-buntansha): TACHIDA Hidenori 九州大学, 大学院・理学研究院, 教授 (70216985) ; SUYAMA Yoshihisa 東北大学, 農学研究科, 准教授 (60282315)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 送粉シンドローム / 花色 / 花香 / 開花時間 / 種分化 / キスゲ属

Research Abstract

We showed that flowering time and flower color of Hemerocallis fulva ; Pollination by swallowtail butterflies and H. citrina ; Pollination by hawkmoths were regulated by major loci using segregation of F2 hybrids. We successful for genotyping individual pollen grains, thus we showed that swallowtail butterflies preferentially visited reddish flowers and hawkmoths preferentially visited yellowish flowers. We evaluated the possibility of the evolution by simulation using our genetic and experimental data. We found that flowering time having pleiotropic effects both on natural selection and assortative mating, and promote evolution of H. citrina from H. fulva.

Research Products

• [Journal Article] Relative role of flower color and scent on pollinator attraction : experimental tests using F1 and F2 hybrids of daylily and nightlily. (2012)
  • Author(s): Hirota SK, Nitta K, Kim Y, Kato A, Kawakubo N, Yasumoto AA, Yahara T.
  • Journal Title: PLoS ONE
  • Peer Reviewed
• [Journal Article] Population genetic structure of a widespread coniferous tree Taxodium distichum[ L.] Rich (2012)
  • Author(s): Tanaka A, Ohtani M, Suyama Y, Inomata N, Tsumura Y, Middleton BA, Tachida H and Kusumi J.
  • Journal Title: (Cupressaceae) in the Mississippi River Alluvial Valley and Florida., Tree Genetics and Genomes
  • Peer Reviewed
• [Journal Article] Pollination biology of Lilium japonicum var. abeanum and var. japonicum : evidence of adaptation to the different availability of diurnal and nocturnal pollinators (2012)
  • Author(s): Yokota S and Yahara T.
  • Journal Title: Plant Species Biology Volume: vol.27 Pages: 96-105
  • Peer Reviewed
• [Journal Article] Population genetic structure of a widespread coniferous tree Taxodium distichum [L.] Rich. (Cupressaceae) in the Mississippi River Alluvial Valley and Florida (2012)
  • Author(s): Tanaka, et al
  • Journal Title: Tree Genetics and Genomes Volume: (accepted)
  • Peer Reviewed
• [Journal Article] Pollination biology of Lilium japonicum var.abeanum and var.japonicum : evidence of adaptation to the different availability of diurnal and nocturnal pollinators (2012)
  • Author(s): Shizuka Yokota, Tetsukazu Yahara
  • Journal Title: Plant Species Biology Volume: 27 Issue: 1 Pages: 96-105
  • DOI: 10.1111/j.1442-1984.2011.00336.x
  • Peer Reviewed
• [Journal Article] Variation of flower opening and closing times in F1 and F2 hybrids of daylily(Hemerocallis fulva ; Hemerocallidaceae) and nightlily(H. citrina). (2010)
  • Author(s): Nitta K, Yasumoto AA, and Yahara T.
  • Journal Title: American Journal of Botany Volume: vol.97 Pages: 261-267
  • Peer Reviewed
• [Journal Article] Genetic diversity assessments in the century of genome science (2010)
  • Author(s): Yahara T, Donoghue M, Zardoya R, Faith D, and Cracraft J.
  • Journal Title: Current Opinion in Environment Sustainability Volume: vol.2 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Genetic structure and putative selective sweep in the pioneer tree (2010)
  • Author(s): Yoshida T, Nagai H, Yahara T, Tachida H.
  • Journal Title: Zanthoxylum ailanthoides, Journal of Plant Research Volume: vol.123 Pages: 607-616
  • Peer Reviewed
• [Journal Article] Variation of flower opening and closing times in F1 and F2 hybrids of daylily (Hemerocallis fulva ; Hemerocallidaceae) and nightlily (H.citrina) (2010)
  • Author(s): Nitta K, Yasumoto AA, Yahara T.
  • Journal Title: American Journal of Botany Volume: 97 Pages: 261-267
  • Peer Reviewed
• [Journal Article] Genetic diversity assessments in the century of genome science (2010)
  • Author(s): Yahara T, Donoghue M, Zardoya R, Faith D, Cracraft J.
  • Journal Title: Current Opinion in Environment Sustainability Volume: 2 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Genetic structure and putative selective sweep in the pioneer tree, Zanthoxylum ailanthoides (2010)
  • Author(s): Yoshida T, Nagai H, Yahara T, Tachida H.
  • Journal Title: Journal of Plant Research Volume: 123 Pages: 607-616
  • NAID: 10027220144
  • Peer Reviewed
• [Journal Article] Relative fitness of females and hermaphrodites in a natural gynodioecious populations of wild radish (2009)
  • Author(s): Miyake K, Miyake T, Terachi T, and Yahara T.
  • Journal Title: Raphanus sativus L.(Brasicaceae): comparison based on molecular genotyping., Journal of Evolutionary Biology Volume: vol.22 Pages: 2012-2019
  • Peer Reviewed
• [Journal Article] Comparative phylogeography of two closely related Viola species occurring in contrasting habitats in the Japanese archipelago (2009)
  • Author(s): Toyama H and Yahara T.
  • Journal Title: Journal of Plant Research Volume: vol.122 Pages: 389-401
  • NAID: 10025180471
  • Peer Reviewed
• [Journal Article] Comparative phylogeography of two closely related Viola species occurring in contrasting habitats in the Japanese archipelago. (2009)
  • Author(s): Toyama H., Yahara T.
  • Journal Title: Journal of Plant Research Volume: 122 Pages: 389-401
  • NAID: 10025180471
  • Peer Reviewed
• [Journal Article] Relative fitness of females and hermaphrodites in a natural gynodioecious populations of wild radish, Raphanus sativus L.(Brasicaceae) : comparison based on molecular genotyping. (2009)
  • Author(s): Miyake K, Miyake T, Terachi T, Yahara T.
  • Journal Title: Journal of Evolutionary Biology Volume: 22 Pages: 2012-2019
  • Peer Reviewed
• [Presentation] Both butterflies and hawkmoths mediate selection on red flower color against yellow : evidence from genotyping individual pollen grains on the stigma. (2012)
  • Author(s): Hirota SK, Nitta K, Suyama Y, Kawakubo N, Yasumoto AA, Yahara T.
  • Organizer: The 5th EAFES International Congress
  • Place of Presentation: 龍谷大学瀬田キャンパス
  • Year and Date: 2012-03-19
• [Presentation] Both butterflies and hawkmoths mediate selection on red flower color against yellow : evidence from genotyping individual pollen grains on the stigma (2012)
  • Author(s): Shun K.Hirota, Kozue Nitta, Yoshihisa Suyama, Nobumitsu Kawakubo, Akiko A.Yasumoto, Tetsukazu Yahara
  • Organizer: The 5th EAFES International Congress
  • Place of Presentation: 龍谷大学瀬田キャンパス
  • Year and Date: 2012-03-19
• [Presentation] Butterflies and hawkmoths mediate selection on floral traits : evidence from genotyping individual pollen grains on the stigma. (2012)
  • Author(s): Hirota SK, Nitta K, Suyama Y, Kawakubo N, Yasumoto AA, Yahara T.
  • Organizer: Conservation and Sustainable Use of Pollinators : towards Global Assessments
  • Place of Presentation: 九州大学箱崎キャンパス
• [Presentation] Butterflies and hawkmoths mediate selection on floral traits : evidence from genotyping individual pollen grains on the stigma (2012)
  • Author(s): Shun K.Hirota, Kozue Nitta, Yoshihisa Suyama, Nobumitsu Kawakubo, Akiko A.Yasumoto, Tetsukazu Yahara
  • Organizer: Conservation and Sustainable Use of Pollinators : towards Global Assessments
  • Place of Presentation: 九州大学箱崎キャンパス
• [Presentation] 、夜咲きの進化.ハマカンゾウとキスゲに関する理論的研究. (2011)
  • Author(s): 松本知高,安元暁子,新田梢,廣田峻,矢原徹一,舘田英典
  • Organizer: 第43回種生物学シンポジウム(招待講演)
  • Place of Presentation: 富士Calm
  • Year and Date: 2011-12-10
• [Presentation] アゲハもスズメガも赤花を選択する?:花粉一粒ジェノタイピングを用いた送粉成功評価 (2011)
  • Author(s): 廣田峻,新田梢,陶山佳久,川窪伸光,安元暁子,矢原徹一
  • Organizer: 第43回種生物学シンポジウム
  • Place of Presentation: 富士Calm
  • Year and Date: 2011-12-10
• [Presentation] 夜咲きの進化～ハマカンゾウとキスゲに関する理論的研究～ (2011)
  • Author(s): 松本知高, 安元暁子, 新田梢, 廣田峻, 矢原徹一, 舘田英典
  • Organizer: 第43回種生物学シンポジウム
  • Place of Presentation: 富士Calm(招待講演)
  • Year and Date: 2011-12-10
• [Presentation] Evolution of sphingophily : a case study of Hemerocallis species. (2011)
  • Author(s): Matsumoto T, Yasumoto AA, Nitta K, Hirota SK, Yahara T, Tachida H.
  • Organizer: SMBE 2011(国際分子進化学会議)
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2011-07-27
• [Presentation] Genetic basis of floweranthocyanins : flower color evolution in daylily(Hemerocallis fulva) and nightlily(H. citrina). (2011)
  • Author(s): Nitta K, Murai Y, Sakaguchi Y, Ozeki Y, Yasumoto AA, Iwashina T, Rausher MD, Yahara T.
  • Organizer: SMBE 2011(国際分子進化学会議)
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2011-07-27
• [Presentation] Genetic basis of flower anthocyanins : flower color evolution in daylily (Hemerocallis fulva) and nightlily (H.citrina) (2011)
  • Author(s): Kozue Nitta, Yoshinori Murai, Yumi Sakaguchi, Yoshihiro Ozeki, Akiko A.Yasumoto, Tsukasa Iwashina, Mark D.Rausher, Tetsukazu Yahara
  • Organizer: SMBE 2011(国際分子進化学会議)
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2011-07-27
• [Presentation] Evolution of sphingophily : a case study of Hemerocallis species (2011)
  • Author(s): Tomotaka Matsumoto, Akiko A.Yasumoto, Kozue Nitta, Shun K.Hirota, Tetsukazu Yahara, Hidenori Tachida
  • Organizer: SMBE 2011(国際分子進化学会議)
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2011-07-27
• [Presentation] キスゲ属における送粉シンドロームに関する花形質の遺伝的基礎 (2011)
  • Author(s): 新田梢,廣田峻,安元暁子,矢原徹一
  • Organizer: 第58回日本生態学会大会(招待講演)
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-03-09
• [Presentation] 、アゲハを介した送粉過程:花粉1粒DNA解析を用いた各花形質の送粉貢献度の測定 (2011)
  • Author(s): 廣田峻,新田梢,陶山佳久,川窪伸光,安元暁子,矢原徹一
  • Organizer: 第58回日本生態学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-03-09
• [Presentation] キスゲ属における送粉シンドロームに関する花形質の遺伝的基礎 (2011)
  • Author(s): 新田梢, 廣田峻, 安元暁子, 矢原徹一
  • Organizer: 第58回日本生態学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-03-09
• [Presentation] アゲハを介した送粉過程:花粉1粒DNA解析を用いた各花形質の送粉貢献度の測定 (2011)
  • Author(s): 廣田峻, 新田梢, 陶山佳久, 川窪伸光, 安元暁子, 矢原徹一
  • Organizer: 第58回日本生態学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-03-09
• [Presentation] キスゲとハマカンゾウにおける花色の違いの遺伝的背景 (2010)
  • Author(s): 新田梢・坂口祐美・三島美佐子・小関良宏・安元暁子・矢原徹一
  • Organizer: 第57回日本生態学会大会[ポスター賞(進化分野)優秀賞受賞]
  • Place of Presentation: 東京大学(駒場)
  • Year and Date: 2010-03-17
• [Presentation] キスゲとハマカンゾウにおける花色の違いの遺伝的背景[ポスター賞(進化分野)優秀賞受賞] (2010)
  • Author(s): 新田梢・坂口祐美・三島美佐子・小関良宏・安元暁子・矢原徹一
  • Organizer: 第57回日本生態学会大会
  • Place of Presentation: 東京大学(駒場)
  • Year and Date: 2010-03-17
• [Presentation] アゲハとスズメガによる花色・花香の選択:花粉一粒PCRを用いた実測 (2010)
  • Author(s): 廣田峻・新田梢・陶山佳久・川窪伸光・安元暁子・矢原徹一
  • Organizer: 第57回日本生態学会大会
  • Place of Presentation: 東京大学(駒場)
  • Year and Date: 2010-03-16
• [Presentation] 、アゲハ媒における各花形質の送粉貢献度:花粉1粒DNA解析を用いた実測 (2010)
  • Author(s): 廣田峻,新田梢,陶山佳久,川窪伸光,安元暁子,矢原徹一
  • Organizer: 第42回種生物学シンポジウム
  • Place of Presentation: 京都大学理学研究科セミナーハウス(京都府)
• [Presentation] Genetic basis of flower color evolution in daylily(Hemerocallis fulva) and nightlily(H. citrina). (2010)
  • Author(s): Nitta K, Sakaguchi Y, Mishima M, Ozeki Y, Yasumoto AA, Yahara T.
  • Organizer: the 26th International Prize for Biology
  • Place of Presentation: つくば国際会議場
• [Presentation] Genetic basis of flower color evolution in daylily (Hemerocallis fulva) and mghtlily (H.citrina) (2010)
  • Author(s): Kozue Nitta, Yumi Sakaguchi, Misako Mishima, Yoshihiro Ozeki, Akiko A.Yasumoto, Tetsukazu Yahara
  • Organizer: the 26th International Prize for Biology
  • Place of Presentation: つくば国際会議場
• [Presentation] アゲハ媒における各花形質の送粉貢献度:花粉1粒DNA解析を用いた実測 (2010)
  • Author(s): 廣田峻, 新田梢, 陶山佳久, 川窪伸光, 安元暁子, 矢原徹一
  • Organizer: 第42回種生物学シンポジウム
  • Place of Presentation: 京都大学理学研究科セミナーハウス(京都府)
• [Presentation] 花色の違いの遺伝的背景を探る:キスゲとハマカンゾウにおける雑種の表現型と色素合成系遺伝子の解析 (2009)
  • Author(s): 新田梢・坂口祐美・三島美佐子・小関良宏・安元暁子・矢原徹一
  • Organizer: 第41回種生物シンポジウム
  • Place of Presentation: 東京都八王子市
  • Year and Date: 2009-12-12
• [Presentation] アゲハ類・スズメガ類による花形質への選択:花粉一粒PCRに基づいた送粉成功の評価 (2009)
  • Author(s): 廣田峻,新田梢,陶山佳久,川窪伸光,安元暁子,矢原徹一
  • Organizer: 第41回種生物シンポジウム
  • Place of Presentation: 東京都八王子市
  • Year and Date: 2009-12-12
• [Presentation] 、キスゲとハマカンゾウの花色の違いの遺伝的背景:雑種の表現型と色素合成系遺伝子の解析 (2009)
  • Author(s): 新田梢・坂口祐美・三島美佐子・小関良宏・安元暁子・矢原徹一
  • Organizer: 第73回日本植物学会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-09-19
• [Presentation] キスゲとハマカンゾウの花色の違いの遺伝的背景:雑種の表現型と色素合成系遺伝子の解析 (2009)
  • Author(s): 新田梢・坂口祐美・三島美佐子・小関良宏・安元暁子・矢原徹一
  • Organizer: 第73回日本植物学会
  • Place of Presentation: 山形大学
  • Year and Date: 2009-09-19
• [Remarks]
  • URL: http://news.sci.kyushu-u.ac.jp/jovtip7as-18/#_18
• [Remarks] 開花時間の遺伝的基礎を明らかにした研究が、九州大学理学部の研究発信webページ「九大理学部ニュース」で紹介された。「花時計は主要遺伝子が制御」2010年4月20日掲載。
• [Remarks] アウトリーチ活動
• [Remarks] 本研究の材料を用いた、高校生による研究活動の指導を行ってきた。福岡県立小倉高等学校(文部科学省SSH指定校)のSS生命科学研究会の野外送粉観察、DNA実験、開花時間の観察の指導を行った。これらの活動を通して、高校生の研究活動への興味や理系進学への意識を高めることに、貢献してきた。