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Study on Two-Component Systems in Plants

Research Project

Project/Area Number 21370018
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagoya University

Principal Investigator

MIZUNO Takeshi  名古屋大学, 生命農学研究科, 教授 (10174038)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords環境応答 / 情報検知 / 情報伝達 / 二成分制御系 / 植物ホルモン / 概日時計 / 進化 / 植物 / シロイヌナズナ / リン酸リレー / サイトカイニン / 光情報伝達
Research Abstract

The so-called Two-Component Systems (TCS) provide us with the paradigm of signal transduction system in prokaryotes, including numerous bacterial species. TCS play important and general roles in signal perception and adaptive response to a variety of environmental stimuli. However, it should be emphasized that, during evolution, plants also have evolved sophisticated TCS, which now play important physiological roles in plant developments, including not only the phytohormone cytokinin and ethylene perception and signal transduction but also the plant circadian clock systems. In order to gain more fundamental insights into the physiological roles of TCS in plants, in this study we employed not only the model plant Arabidopsis thaliana, but also the model legume Lotus japonicus and the primitive land moss physcomitrella patens. As the results, we gained a number of new insights into the plant TCS, particularly, with regard to the mechanisms underlying cytokinin signal transduction and circadian clock. These results have already been published in a series of reports, as many as 30.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (62 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (41 results) (of which Peer Reviewed: 41 results) Presentation (4 results) Book (14 results) Remarks (3 results)

  • [Journal Article] Molecular mechanism of circadian rhythm in Lotus japonicus is compatible enough with that of Arabidopsis thaliana to robustly regulate heterologous core clock genes2012

    • Author(s)
      Ueoka-Nakanishi, H., *Yamashino, T., Mizuno, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional repressor PRR5 directly regulates clock-output pathhways2012

    • Author(s)
      Nakamichi, N., Kiba, T., Mizuno, T.
    • Journal Title

      P.N.S.A.

      Volume: 109 Pages: 17123-17128

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Circadian clock and PIF4-controlled plant growth: A coincidence mechanism directly integrates a hormones-signaling network into the photoperiodic control of plant architectures in Arabidopsis thaliana.2012

    • Author(s)
      Nomoto, Y., Kubozono, S., *Yamashino, T., Mizuno, T.
    • Journal Title

      Plant Cell Physiol

    • NAID

      10031127303

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A circadian clock and PIF4-mediated double coincidence mechanism is implicated in the thermo-sensitive photoperiodic control of plant architectures in Arabidopsis thaliana2012

    • Author(s)
      Nomoto, Y., *Yamashino, T., Mizuno, T.
    • Journal Title

      Plant Cell Physiol.

    • NAID

      10031127304

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of circadian rhythm in Lotus japonicus and Arabidopsis thaliana are sufficiently compatible to regulate heterologous core clock genes robustly.2012

    • Author(s)
      Ueoka-Nakanishi et. al.,
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 76 Pages: 2332-2334

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional repressor PRR5 directly regulates clock-output pathways.2012

    • Author(s)
      Nakamichi et. al.,
    • Journal Title

      PNAS

      Volume: 109 Issue: 42 Pages: 17123-17128

    • DOI

      10.1073/pnas.1205156109

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Circadian clock- and PIF4-controlled plant growth:2012

    • Author(s)
      Nomoto et al.,
    • Journal Title

      Plant Cell Physiol.

      Volume: 53 Issue: 11 Pages: 1950-1964

    • DOI

      10.1093/pcp/pcs137

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A circadian clock- and PIF4-mediated double coincidence mechanism is implicated in the thermosensitive photoperiodic control of plant architectures in Arabidopsis.2012

    • Author(s)
      Nomoto et al.,
    • Journal Title

      Plant Cell Physiol.

      Volume: 53 Issue: 11 Pages: 1965-1973

    • DOI

      10.1093/pcp/pcs141

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pseudo-response regulator (PRR) homologues of the moss Physcomitrella patens2011

    • Author(s)
      Satbhai, S.B., Mizuno, T., *Aoki, S
    • Journal Title

      DNA Res.

      Volume: 18 Pages: 39-52

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heterologous expression and functional characterization of a Physcomitrella pseudo response regulator Homolog in Arabidopsis2011

    • Author(s)
      Satbhai, S.B., Mizuno, T., *Aoki, S
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 75 Pages: 786-789

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the conserved phosphorylation site in the Aspergillus nidulans response regulator2011

    • Author(s)
      Hagiwara, D. Mizuno, T.
    • Journal Title

      SrrA. Curr. Genet.

      Volume: 57 Pages: 103-114

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of HY5 homolog genes in Physcomitrella patens.2011

    • Author(s)
      Yamawaki, S., :Yamashini, T., Mizuno, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 75 Pages: 1533-1538

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] PIF4 and 5 activate the homeobox ATHB2 gene in the coincident mechanism underlying photoperiodic control of plant growth of A. thaliana.2011

    • Author(s)
      Kunihiro, A., *Yamashina, T., Mizuno, T.
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Pages: 13151329-13151329

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Light-Responsive double B-boxn transcription factors are conserved in Physcomitrella patens.2011

    • Author(s)
      Yamawaki, S., *Yamashino, T., Mizuno, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 75 Pages: 2037-2041

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of shade avoidance responses in Lotus japonicas2011

    • Author(s)
      Nakanishi, H., *Yamashino, T., Mizuno, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 75 Pages: 2148-2154

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heterologous Expression and Functional Characterization of A Physcomitrella Pseudo Response Regulator Homolog, PpPRR2, in Arabidopsis2011

    • Author(s)
      Sabhai, S., 他
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 75 Pages: 786-789

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PRR 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock.2010

    • Author(s)
      Nakamichi, N., Kiba, T., Mizuno, T
    • Journal Title

      Plant Cell

      Volume: 22 Pages: 596-605

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization of plant hormone cytokinin receptor histidine kinases using microorganisms.2010

    • Author(s)
      Mizuno, T. *Yamashino, T.
    • Journal Title

      Methods Enzymol

      Volume: 471 Pages: 101-109

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genomewide characterization of the light-responsive and clock-controlled output pathways in Lotus japonicus.2010

    • Author(s)
      Ono, N., *Yamashino, T., Mizuno, T.
    • Journal Title

      Plant Cell Physiol

      Volume: 51 Pages: 1800-1814

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Data assimilation constrain new connections ad components is a complex, eukaryotic circadian clock model.2010

    • Author(s)
      Pokhiko, H., Hodge, S. Mizuno, T
    • Journal Title

      Mol. Syst. Biol.

      Volume: 6 Pages: 416-416

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] PIF4 is implicated in the regulation of hypocotyl elongation in response to blue light in A. thaliana.2010

    • Author(s)
      Kunihiro, A., *Yamashino, T., Mizuno, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 74 Pages: 2538-2541

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Classification of the genes involved in the two-component system of the mossPhyscomitrella patens.2010

    • Author(s)
      Ishida K., *Yamashino, T., Mizuno, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 74 Pages: 2542-2545

    • NAID

      10027728585

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock2010

    • Author(s)
      Nakamichi, N., 他
    • Journal Title

      Plant Cell

      Volume: 22 Pages: 594-605

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Classification of the genes involved in the two-component system of the moss Physcomitrella patens2010

    • Author(s)
      Ishida, K., 他
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74 Pages: 2542-2545

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomewide characterization of the light-responsive and clock-controlled output pathways in Lotus japonicus2010

    • Author(s)
      Ono, N., 他
    • Journal Title

      Plant Cell Physiol

      Volume: 51 Pages: 1800-1814

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Data assimilation constrain new connections ad components is a complex, eukaryotic circadian clock model2010

    • Author(s)
      Pokhiko, K., 他
    • Journal Title

      Mol.Syst.Biol.

      Volume: 6 Pages: 416-425

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PIF4 and PIF5 are implicated in the regulation of hypocotyl elongation in response to blue light in Arabidopsis thaliana2010

    • Author(s)
      Kunihiro, A., 他
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 72 Pages: 2538-2541

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PRR homologues of the moss Physcomitrella patens : insights into the evolution of the PRR family in land plants2010

    • Author(s)
      Sabhai, S., 他
    • Journal Title

      DNA Res.

      Volume: 18 Pages: 39-52

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A genetic study of the Arabidopsiscircadian clock with reference to the TOC1 gene.2009

    • Author(s)
      Ishida K., *Yamashino, T., Mizuno, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 290-303

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcript profiling of an Arabidopsis PSUEDO RESPONSE REGULATOR arrhythmic triple mutant reveals a role for the circadian clock in cold stress response.2009

    • Author(s)
      Nakamichi, N., Kusano, M., Mizuno, T.
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Pages: 447-462

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Circadian clock regulates photoperiodic response of hypocotyl elongation through a coincidence mechanism in A. thaliana.2009

    • Author(s)
      Niwa, Y., *Yamashino, T., Mizuno, T
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 838-854

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impact of clock-associated Arabidopsis pseudo-response regulators in metabolic coordination.2009

    • Author(s)
      Fukushima, A., Mizuno, T.
    • Journal Title

      Proc. Natl. Acad. Sci

      Volume: 106 Pages: 7251-7256

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] C characterization of NikA histidine kinase and two response regulators with special reference to osmotic adaptation and asexual development in Aspergillus nidulans.2009

    • Author(s)
      Hagiwara, D., Mizuno, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 73

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Linkage between circadian clock and tricarboxylic acid cycle in Arabidopsis2009

    • Author(s)
      Nakamichi, N., Mizuno, T.
    • Journal Title

      Plant Signal Behav

      Volume: 4 Pages: 660-662

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Trandcriptional profiling for Aspergillus nidulans HOGA MAPK signaling pathway in response to fluodioxonil and osmotic stress.2009

    • Author(s)
      Hagiwara, D., Mizuno, T.
    • Journal Title

      Fungal Gen. Biol.

      Volume: 46 Pages: 868-878

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A genome-wide compilation of the two-component systems in Lotus japonicus.2009

    • Author(s)
      Ishida, K., *Yamashino, T., Mizuno, T
    • Journal Title

      DNA Res.

      Volume: 16 Pages: 237-247

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Arabidopsis B-box zinc finger family2009

    • Author(s)
      Khanna, R., Mizuno, T..
    • Journal Title

      Plant Cell

      Volume: 21 Pages: 3416-3420

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cytokinin sensing systems using microorganisms.2009

    • Author(s)
      Higuchi, M., Kakimoto, T., Mizuno, T.
    • Journal Title

      Methods Mol. Biol.

      Volume: 495 Pages: 101-109

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Circadian clock regulates photoperiodic response of hypocotyl elongation through a coincidence mechanism in Arabidopsis thaliana2009

    • Author(s)
      Niwa, Y., et al.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 838-854

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcript profiling of an Arabidopsis PSUEDO RESPONSE REGULATOR arrhythmic triple mutant reveals a role for the circadian clock in cold stress response2009

    • Author(s)
      Nakamichi, N., et al.
    • Journal Title

      Plant Cell Physiol 50

      Pages: 447-462

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A genome-wide compilation of the two-component systems in Lotus japonicus2009

    • Author(s)
      Ishida, K., et al.
    • Journal Title

      DNA Res. 16

      Pages: 237-247

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 日長と気温変化(季節)に応答した胚軸や葉身の伸長制御機構2013

    • Author(s)
      野本友司 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] ミヤコグサにおける概日時計機構の多面的解析2013

    • Author(s)
      石田 快 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] ゲノム情報を通したミヤコグサの花成制御機構に関する検証2013

    • Author(s)
      山篠貴史 他
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] ミヤコグサ光情報伝達系の包括的解析2010

    • Author(s)
      小野奈津子, 他
    • Organizer
      植物生理学会大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-19
    • Related Report
      2009 Annual Research Report
  • [Book] 生物学事典(第5版)2013

    • Author(s)
      水野 猛
    • Publisher
      岩波書店
    • Related Report
      2011 Final Research Report
  • [Book] 図説分子病態学(5版)2013

    • Author(s)
      水野 猛
    • Publisher
      中外医学社
    • Related Report
      2011 Final Research Report
  • [Book] 生物学事典(第5版)2013

    • Author(s)
      水野 猛 (分担)
    • Total Pages
      2171
    • Publisher
      岩波書店
    • Related Report
      2011 Annual Research Report
  • [Book] 二成分制御系研究を振り返って(前編:組換え DNA 時代) 「化学と生物」2011

    • Author(s)
      水野 猛
    • Related Report
      2011 Final Research Report
  • [Book] 二成分制御系研究を振り返って(後編:ゲノム時代) 「化学と生物」2011

    • Author(s)
      水野 猛
    • Related Report
      2011 Final Research Report
  • [Book] 生物学事典2010

    • Author(s)
      水野 猛
    • Publisher
      東京化学同人
    • Related Report
      2011 Final Research Report
  • [Book] 植物の時計「植物のシグナル伝達」2010

    • Author(s)
      山篠貴史 水野 猛
    • Publisher
      共立出版
    • Related Report
      2011 Final Research Report
  • [Book] Methods Enzymol2010

    • Author(s)
      Mizuno, T., Yamashino, T.
    • Total Pages
      508
    • Publisher
      Biochemical characterization of plant hormone cytokinin receptor histidine kinases using microorganisms
    • Related Report
      2010 Annual Research Report
  • [Book] 生物学辞典2010

    • Author(s)
      水野猛(分担執筆)
    • Total Pages
      650
    • Publisher
      東京化学同人
    • Related Report
      2010 Annual Research Report
  • [Book] 植物ゲノム科学辞典2009

    • Author(s)
      水野 猛
    • Publisher
      朝倉書店
    • Related Report
      2011 Final Research Report
  • [Book] 植物の成長を制御するサイトカイニン情報伝達機構2009

    • Author(s)
      山篠貴史 水野 猛
    • Related Report
      2011 Final Research Report
  • [Book] A Stressful Episode in Plant Biology2009

    • Author(s)
      水野 猛 山篠貴史
    • Related Report
      2011 Final Research Report
  • [Book] 植物の概日時計分子機構「光周性の分子生物学」2009

    • Author(s)
      水野 猛 山篠貴史
    • Publisher
      シュプリンガージャパン
    • Related Report
      2011 Final Research Report
  • [Book] 「光周性の分子生物学」/植物の概日時計分子機構2009

    • Author(s)
      水野猛, 山篠貴史
    • Total Pages
      210
    • Publisher
      シュプリンガージャパン
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.agr.nagoya-u.ac.jp/~microbio/

    • Related Report
      2011 Final Research Report
  • [Remarks] 名古屋大学生命農学研究科ゲノム情報機能学研究室

    • URL

      http://www.agr.nagoya-u.ac.jp/~microbio/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.nagoya-u.ac.jp/~microbio/

    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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