Elucidation of molecular mechanisms underlying hyper sensitization of phytochrome A

• Project/Area Number: 21370020
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: NAGATANI Akira 京都大学, 大学院・理学研究科, 教授 (40183082)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2009: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: フィトクロム / 光応答 / シグナル伝達 / 環境応答 / 蛋白構造

Research Abstract

Plants have a specialized phytochrome, phyA, in addition to conventional phytochromes represented by phyB. We expressed chimeric phytochromes having a phyA or phyB sequence in each of 4 phytochrome domains, N-PAS, GAF, PHY and the C-terminus in transgenic Arabidopsis. Biochemical, cell biological and physiological analysis of those plants revealed a "modular" structure of phyA in which different phyA specific properties base on distinct parts of the molecule.

Research Products

• [Journal Article] Mutations in N-terminal Flanking Region of Blue Light-sensing Light-Oxygen and Voltage 2 (LOV2) Domain Disrupt Its Repressive Activity on Kinase Domain in the Chlamydomonas Phototropin (2012)
  • Author(s): Yusuke Aihara, Takaharu Yamamoto, Koji Okajima, Kazuhiko Yamamoto, Tomomi Suzuki, Satoru Tokutomi, Kazuma Tanaka, Akira Nagatani
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 13 Pages: 9901-9909
  • DOI: 10.1074/jbc.m111.324723
  • Peer Reviewed
• [Journal Article] Tissue-Autonomous Promotion of Palisade Cell Development by Phototropin 2 in Arabidopsis (2011)
  • Author(s): Kozuka T., et al
  • Journal Title: Plant Cell Volume: 23 Issue: 10 Pages: 3684-3695
  • DOI: 10.1105/tpc.111.085852
  • Peer Reviewed
• [Journal Article] Phytochrome : structural basis for its functions (2010)
  • Author(s): A. Nagatani
  • Journal Title: Curr. Opin. Plant Biol. Volume: 13 Issue: 5 Pages: 565-570
  • DOI: 10.1016/j.pbi.2010.07.002
  • Peer Reviewed
• [Journal Article] Sub cellular sites of the signal transduction and degradation of phytochrome A (2010)
  • Author(s): Toledo-Ortiz, G., Y. Kiryu, J. Kobayashi1, Y. Kim, H. G. Nam, N. Mochizuki and A. Nagatani
  • Journal Title: Plant Cell Physiol. Volume: 51 Issue: 10 Pages: 1648-1660
  • DOI: 10.1093/pcp/pcq121
  • NAID: 10027353109
  • Peer Reviewed
• [Journal Article] Arabidopsis PHYTOCHROME INTERACTING FACTOR proteins promote phytochrome B polyubiquitination by COP1 E3 ligase in the nucleus (2010)
  • Author(s): Jang, I. C., R. Henriques, H. S. Seo, A. Nagatani and N. H. Chua
  • Journal Title: Plant Cell Volume: 22 Issue: 7 Pages: 2370-2383
  • DOI: 10.1105/tpc.109.072520
  • Peer Reviewed
• [Journal Article] Involvement of auxin and brassinosteroid in the regulation of petiole elongation under the shade. (2010)
  • Author(s): Kozuka, T., J. Kobayashi, G. Horiguchi, T. Demura, H. Sakakibara, H. Tsukaya and A. Nagatani
  • Journal Title: Plant Physiol. Volume: 153 Issue: 4 Pages: 1608-1618
  • DOI: 10.1104/pp.110.156802
  • Peer Reviewed
• [Journal Article] Involvement of auxin and brassinosteroid in the regulation of petiole elongation under the shade (2010)
  • Author(s): Kozuka, T., J.Kobayashi, G.Horiguchi, T.Demura, H.Sakakibara, H.Tsukaya, A.Nagatani
  • Journal Title: Plant Physiol Volume: 153 Pages: 1608-1618
  • Peer Reviewed
• [Journal Article] Subcellular sites of the signal transduction and degradation of phytochrome A (2010)
  • Author(s): Toledo-Ortiz, G., Y.Kiryu, J.Kobayashi, Y.Kim, H.G.Nam, N.Mochizuki, A.Nagatani
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 1648-1660
  • NAID: 10027353109
  • Peer Reviewed
• [Journal Article] Arabidopsis PHYTOCHROME INTERACTING FACTOR proteins promote phytochrome B polyubiquitination by COP1 E3 ligase in the nucleus. (2010)
  • Author(s): Jang, I.C., R.Henriques, H.S.Seo, A.Nagatani, N.H.Chua
  • Journal Title: Plant Cell Volume: 22 Pages: 2370-2383
  • Peer Reviewed
• [Journal Article] Subcellular sites of the signal transduction and degradation of phytochrome A (2010)
  • Author(s): Toledo-Ortiz, G., Y.Kiryu, J.Kobayashil, Y.Kim, H.G.Nam, N.Mochizuki, A.Nagatani
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 1648-1660
  • NAID: 10027353109
  • Peer Reviewed
• [Journal Article] Phytochrome : structural basis for its functions. (2010)
  • Author(s): Nagatani, A
  • Journal Title: Curr.Opin.Plant Biol. Volume: 13 Pages: 565-570
  • Peer Reviewed
• [Journal Article] Residues clustered in the light-sensing knot of phytochrome B are necessary for conformer-specific binding to signaling partner PIF3 (2009)
  • Author(s): Kikis, E. A., Y. Oka, M. E. Hudson, A. Nagatani and P. H. Quail
  • Journal Title: PLoS Genet Volume: 5 Issue: 1 Pages: e1000352-e1000352
  • DOI: 10.1371/journal.pgen.1000352
  • NAID: 130006994270
  • Peer Reviewed
• [Journal Article] Residues clustered in the light-sensing knot of phytochrome B are necessary for conformer-specific binding to signaling partner PIF3. (2009)
  • Author(s): Kikis, E.A., Y.Oka, M.E.Hudson, A.Nagatani, P.H.Quail
  • Journal Title: PLoS Genet Volume: 5
  • NAID: 130006994270
  • Peer Reviewed
• [Presentation] Modular structure of phytochrome A (2012)
  • Author(s): Akira Nagatani
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: 東大寺総合文化センター(奈良県)
  • Year and Date: 2012-03-19
• [Presentation] Modular structure of phytochrome A (2012)
  • Author(s): Akira Nagatani
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: 東大寺総合文化センター(奈良県)(招待講演)
  • Year and Date: 2012-03-19
• [Presentation] フォトトロピンのLOV2領域・N末端側近傍の変異によりキナーゼ活性の抑制機能が失われる (2012)
  • Author(s): 相原悠介, ほか
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-17
• [Presentation] MALDI-FTMSを用いたシロイヌナズナ光応答のメタボローム解析 (2012)
  • Author(s): 小塚俊明, ほか
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] フィトクロムAの作用機構の生化学的解析 (2012)
  • Author(s): 後藤一也, ほか
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] Mg-Chelatase Hサブユニット(CHLH/GUN5)の構造・機能解析を中心とした、プラスチドシグナル伝達の解析 (2012)
  • Author(s): 衣幡春映, ほか
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] The molecular mechanism of growth regulation by phytochrome (2011)
  • Author(s): Akira Nagatani
  • Organizer: Strategies of Plants against Global Environmental Change
  • Place of Presentation: 倉敷市芸文館(岡山県)
  • Year and Date: 2011-12-09
• [Presentation] The molecular mechanism of growth regulation by phytochrome (2011)
  • Author(s): Akira Nagatani
  • Organizer: Strategies of Plants against Global Environmental Change
  • Place of Presentation: 倉敷市芸文館(岡山県)(招待講演)
  • Year and Date: 2011-12-09
• [Presentation] フィトクロムの生理応答の時空間的解析 (2011)
  • Author(s): 長谷あきら
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2011-09-21
• [Presentation] フィトクロムの生理応答の時空間的解析 (2011)
  • Author(s): 長谷あき, ら
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都府)(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] The structural basis for phyA-specific functions (2011)
  • Author(s): Akira Nagatani
  • Organizer: 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良県新公会堂(奈良県)
  • Year and Date: 2011-07-31
• [Presentation] The structural basis for phyA-specific functions (2011)
  • Author(s): Akira Nagatani
  • Organizer: 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良県新公会堂(奈良県)(招待講演)
  • Year and Date: 2011-07-31
• [Presentation] Spatio-temporal regulatory network controlling stem elongation under the shade (2011)
  • Author(s): Akira Nagatani
  • Organizer: Photoperiodism and Circadian Clock Function in Plant Development
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2011-05-05
• [Presentation] Spatio-temporal regulatory network controlling stem elongation under the shade (2011)
  • Author(s): Akira Nagatani
  • Organizer: Interplay of Light, Photoperiodism and Circadian Clock Function in Plant Development
  • Place of Presentation: Barcelona, Spain(招待講演)
  • Year and Date: 2011-05-05
• [Presentation] シロイヌナズナの避陰応答における器官間光シグナル伝達機構の解析 (2010)
  • Author(s): 小林淳子, ほか
  • Organizer: 第16回日本光生物学協会年会
  • Place of Presentation: 大阪大学銀杏会館(大阪府)
  • Year and Date: 2010-08-11
• [Presentation] Structural basis of phytochrome A specific functions (2010)
  • Author(s): Akira Nagatani
  • Organizer: Plant Signalling Mechanisms
  • Place of Presentation: University of Freiburg (Germany)(招待講演)
  • Year and Date: 2010-06-26
• [Presentation] Structural basis of phytochrome A specific functions, Symposium (2010)
  • Author(s): Akira Nagatani
  • Organizer: Plant Signalling Mechanisms on the occasion of the retirement of Eberhard Schafer
  • Place of Presentation: Freiburg, Germany
  • Year and Date: 2010-06-25
• [Presentation] Structural basis of phytochrome A specific functions (2010)
  • Author(s): A.Nagatani
  • Organizer: Symposium "Plant Signalling Mechanisms"on the occasion of the retirement of Eberhard Schafer
  • Place of Presentation: Freiburg, Germany
  • Year and Date: 2010-06-25
• [Presentation] an organizer of the Plenary Session 2 : Cell Biology (2010)
  • Author(s): Akira Nagatani
  • Organizer: 21st International conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-06-06
• [Presentation] 青色光受容体photによる低分子量Gタンパク質ARF1の制御 (2010)
  • Author(s): 鈴木友美, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] プラスチドシグナル伝達におけるテトラぴロール合成の関わり (2010)
  • Author(s): 望月伸悦, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] phyBシグナル伝達経路に関わる新奇因子PHL (2010)
  • Author(s): 遠藤求, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] ミオシンは光と重力に対する環境応答のブレーキとして働く (2010)
  • Author(s): 岡本圭史, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] 青色光受容体フォトトロピンの"Flippase-kinase"としての役割の解明 (2010)
  • Author(s): 相原悠介, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] AGCVIIIキナーゼ過剰発現植物体のフォトトロピン表現型の解析 (2010)
  • Author(s): 山本和彦, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] 本葉扁平性を制御するフィトクロム生理機能の発見 (2010)
  • Author(s): 小塚俊明, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] シロイヌナズナの避陰応答における器官間光シグナル伝達の解析 (2010)
  • Author(s): 小林淳子, ほか
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] Structural basis for phyA-specific functions (2009)
  • Author(s): Y. Ono, Y. Oka, et al.
  • Organizer: Memorial Symposium for the 25th International Prize for Biology
  • Place of Presentation: 京都大学芝蘭会館(京都府)
  • Year and Date: 2009-12-02
• [Presentation] Phototropin-mediated regulation of polar elongation in palisade-tissue Cells (2009)
  • Author(s): Toshiaki Kozuka & Akira Nagatani
  • Organizer: Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
  • Place of Presentation: 京都大学芝蘭会館(京都府)
  • Year and Date: 2009-12-02
• [Presentation] Plastid-to-nucleus signaling, Memorial Symposium for the 25th International Prize for Biology (2009)
  • Author(s): Nobuyoshi Mochizuki & Akira Nagatani
  • Organizer: Biology of Sensing
  • Place of Presentation: 京都大学芝蘭会館(京都府)
  • Year and Date: 2009-12-02
• [Presentation] A structure/function study of phototropins (2009)
  • Author(s): Akira Nagatani
  • Organizer: Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
  • Place of Presentation: 京都大学芝蘭会館(京都府)
  • Year and Date: 2009-12-02
• [Presentation] A structure/function study of phototropins (2009)
  • Author(s): A.Nagatani
  • Organizer: Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
  • Place of Presentation: 京都大学 芝蘭会館(京都府)
  • Year and Date: 2009-12-02
• [Presentation] Plastid-to-nucleus signaling (2009)
  • Author(s): N.Mochizuki, A.Nagatani
  • Organizer: Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
  • Place of Presentation: 京都大学 芝蘭会館(京都府)
  • Year and Date: 2009-12-02
• [Presentation] Phototropin-mediated regulation of polar elongation in palisade-tissue Cells (2009)
  • Author(s): T.Kozuka, A.Nagatani
  • Organizer: Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
  • Place of Presentation: 京都大学 芝蘭会館(京都府)
  • Year and Date: 2009-12-02
• [Presentation] Structural basis for phyA-specific functions (2009)
  • Author(s): Y.Ono, Y.Oka, et al.
  • Organizer: Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
  • Place of Presentation: 京都大学 芝蘭会館(京都府)
  • Year and Date: 2009-12-02
• [Presentation] 植物の光応答機構-光受容体理解に向けた3つのアプローチ (2009)
  • Author(s): 長谷あきら
  • Organizer: 2009年日本植物学会近畿支部大会
  • Place of Presentation: 神戸大学(兵庫県)
  • Year and Date: 2009-11-14
• [Presentation] フォトトロピンによる光応答～光受容から生理応答まで (2009)
  • Author(s): 長谷あきら
  • Organizer: 科学研究費特定領域研究「LOV光受容体による植物の運動制御機構」報告会
  • Place of Presentation: 京都リサーチパーク(京都府)
  • Year and Date: 2009-09-26
• [Presentation] 避陰応答における組織/器官間シグナル伝達の研究 (2009)
  • Author(s): 小林淳子ほか
  • Organizer: 第15回日本光生物学協会年会
  • Place of Presentation: 自然科学研究機構岡崎コンフェレンスセンター(愛知県)
  • Year and Date: 2009-08-19
• [Presentation] Structural basis of phytochrome A-specific functions (2009)
  • Author(s): Yoshito Oka, et al.
  • Organizer: The 3rd International Symposium of the Biodiversity and Evolution
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2009-07-24
• [Presentation] Inter-organ/tissue communication in the shade avoidance response (2009)
  • Author(s): Junko Kobayashi, et al.
  • Organizer: The 3rd International Symposium of the Biodiversity and Evolution
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2009-07-24
• [Presentation] Possible roles of plant's blue-light receptor, phototropin, as a "flippase-kinase" (2009)
  • Author(s): Yusuke Aihara, et al.
  • Organizer: The 3rd International Symposium of the Biodiversity and Evolution
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2009-07-24
• [Presentation] Possible roles of plant's blue-light receptor, phototropin, as a "flippase-kinase". (2009)
  • Author(s): Y.Aihara, et al.
  • Organizer: The 3rd International Symposium of the Biodiversity and Evolution
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2009-07-24
• [Presentation] Inter-organ/tissue communication in the shade avoidance response (2009)
  • Author(s): J.Kobayashi, et al.
  • Organizer: The 3rd International Symposium of the Biodiver sity and Evolution
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2009-07-24
• [Presentation] Structural basis of properites specific to phytochrome A (2009)
  • Author(s): Akira Nagatani
  • Organizer: Seminar
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2009-07-15
• [Presentation] Structural basis of phyA-specific properties (2009)
  • Author(s): Akira Nagatani
  • Organizer: SEB Annual Main Meeting
  • Place of Presentation: Glasgow, UK
  • Year and Date: 2009-06-28
• [Presentation] Complex hierarchy of the shade avoidance response (2009)
  • Author(s): Akira Nagatani
  • Organizer: 15th International Congres of Photobiology
  • Place of Presentation: Dusseldorf, Germany
  • Year and Date: 2009-06-18
• [Presentation] Complex hierarchy of the shade avoidance response. (2009)
  • Author(s): A.Nagatani
  • Organizer: 15th International Congres of Photobiology
  • Place of Presentation: Dusseldorf, Germany
  • Year and Date: 2009-06-18
• [Remarks]
  • URL: http://physiol2.bot.kyoto-u.ac.jp/~nagatani/HP3/
• [Remarks]
  • URL: http://www.bot.kyoto-u.ac.jp/j/index.htmlhttp://www.bot.kyoto-u.ac.jp/j/index.html
• [Remarks]
  • URL: http://physiol2.bot.kyoto-u.ac.jp/~nagatani/HP3/
• [Remarks]
  • URL: http://physiol2.bot.kyoto-u.ac.jp/~nagatani/HP3/
• [Remarks]
  • URL: http://physiol2.bot.kyoto-u.ac.jp/~nagatani/HP3/