Mechanism for cytokinin signal transduction by the response regulator ARR1

• Project/Area Number: 21370021
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: AOYAMA Takashi 京都大学, 化学研究所, 教授 (80202498)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 植物ホルモン / 転写因子 / シグナル伝達 / 細胞分化 / サイトカイニン / オーキシン / 転写誘導 / 標的遺伝子 / 細胞増殖 / 転写 / 2成分制御系

Research Abstract

Arabidopsis ARR1 is a response regulator which activates the transcription of primary responsive genes to cytokinin. In chromosome immunoprecipitation analysis, it was confirmed that an ARR1 fusion protein was bound to the promoter region of an ARR1-direct-target gene, ARR6. The binding was enhanced by the treatment of plants with cytokinin. It was suggested that ARR1 widely covers the chromosome DNA. In addition to the ARR1 study, a monitoring system was developed, by which the intensity of cytokinin signals in individual cells is visualized taking advantage of an artificial cytokinin-responsive promoter.

Research Products

• [Journal Article] AtSap130/AtSF3b-3 function is required for reproduction in Arabidopsis thaliana (2011)
  • Author(s): Aki, S., Nakai, H., Aoyama, T., Oka, A., and Tsuge, T
  • Journal Title: Plant Cell Physiol Volume: 52 Issue: 8 Pages: 1330-1339
  • DOI: 10.1093/pcp/pcr077
  • NAID: 10029713258
  • Peer Reviewed
• [Journal Article] D-myo-inositol-3-phosphate affects phosphatidylinositol-mediated end membrane function in Arabidopsis and is essential for auxin-regulated embryogenesis (2011)
  • Author(s): Luo, Y., Qin, G., Zhang, J., Liang, Y., Song, Y., Zhao, M., Tsuge, T., Aoyama, T., Liu, J., Gu, H., and Qu, L.-J
  • Journal Title: Plant Cell Volume: 23 Pages: 1352-1372
  • Peer Reviewed
• [Journal Article] AtSap130/AtSF3b-3 function is required for reproduction Arabidopsis thaliana (2011)
  • Author(s): Aki, S., et al
  • Journal Title: Plant & Cell Physiology Volume: 52 Issue: 4 Pages: 1330-1339
  • DOI: 10.1105/tpc.111.083337
  • Peer Reviewed
• [Journal Article] A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality (2010)
  • Author(s): She, K.-C., Kusano, H., Koizumi, K., Yamakawa, H., Hakata, M., Imamura, T., Fukuda, M., Naito, N., Tsurumaki, Y., Yaeshima, M., Tsuge, T., Matsumoto, K., Kudoh, M., Itoh, E., Kikuchi, SKishimoto, N., Yazaki, J., Ando, T., Yano, M., Aoyama, T., Sasaki, T.,
  • Journal Title: Plant Cell Volume: 22 Pages: 3280-3294
  • Peer Reviewed
• [Journal Article] Control of end reduplication of trichome by RPT2a, a subunit of the 19S proteasome in Arabidopsis (2010)
  • Author(s): Sato, K., Maki, Y., Imai, K. K., Aoyama, T., Goto, D. B., and Yamaguchi, J
  • Journal Title: J Plant Res Volume: 123 Issue: 5 Pages: 701-706
  • DOI: 10.1007/s10265-010-0321-x
  • Peer Reviewed
• [Journal Article] Involvement of Arabidopsis thaliana phospholipase D・2 in root hydrotropism through the seuppression of root gravitropism (2010)
  • Author(s): Taniguchi, Y. Y., Taniguchi, M., Tsuge, T., Oka, A., and Aoyama, T
  • Journal Title: Planta Volume: 231 Pages: 491-497
  • Peer Reviewed
• [Journal Article] Control of endoreduplication of trichome by RPT2a, a subunit of the 19S proteasome in Arabidopsis (2010)
  • Author(s): Sato, K., et al
  • Journal Title: J.Plant Res. Volume: 123 Pages: 701-706
  • Peer Reviewed
• [Journal Article] A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality. (2010)
  • Author(s): She, K.-C., et al.
  • Journal Title: Plant Cell Volume: 10 Pages: 3280-3294
  • Peer Reviewed
• [Journal Article] Control of endoreduplication of trichome by RPT2a, a subunit of the 19S proteasome in Arabidopsis. (2010)
  • Author(s): Sato, K., et al.
  • Journal Title: J.Plant Res. Volume: 123 Pages: 701-706
  • Peer Reviewed
• [Journal Article] Involvement of Arabidopsis thaliana phospholipase Dζ2 in root hydrotropism through the suppression of root gravitropism. (2010)
  • Author(s): Taniguchi, Y.Y., et al.
  • Journal Title: Planta Volume: 231 Pages: 491-497
  • Peer Reviewed
• [Presentation] リン酸欠乏に応答した根毛伸長におけるPIP5Kの機能 (2012)
  • Author(s): 和田悠貴香、安田敬子、柘植知彦、青山卓史
  • Organizer: 日本植物生理学会・2012年度年会
  • Place of Presentation: 京都産業大学、京都府
• [Presentation] リン酸欠乏に応答した根毛伸長におけるPIP5Kの機能 (2012)
  • Author(s): 和田悠貴香, ら
  • Organizer: 日本植物生理学会・2012年度年会
  • Place of Presentation: 京都産業大学
• [Presentation] 根毛をもたないシロイヌナズナ変異株NR23の分子生理学的な特徴 (2012)
  • Author(s): 田中奈月, ら
  • Organizer: 日本植物生理学会・2012年度年会
  • Place of Presentation: 京都産業大学
• [Presentation] 植物細胞形態形成におけるリン脂質シグナルの役割 (2011)
  • Author(s): 青山卓史
  • Organizer: 静岡大学・若手グローバル研究リーダー育成プログラムシンポジウム
  • Place of Presentation: 静岡大学、静岡県
  • Year and Date: 2011-09-15
• [Presentation] 植物細胞形態形成におけるリン脂質シグナルの役割 (2011)
  • Author(s): 青山卓史
  • Organizer: 静岡大学・若手グローバル研究リーダー育成プログラムシンポジウム
  • Place of Presentation: 静岡大学(招待講演)
  • Year and Date: 2011-09-15
• [Presentation] シロイヌナズナ・ホスホリパーゼDζ1遺伝子の遺伝学的解析 (2011)
  • Author(s): 安斎尚子、大橋洋平、谷口雅俊、柘植知彦、青山卓史
  • Organizer: 日本植物生理学会・2011年度年会
  • Place of Presentation: 日本植物生理学会ホームページ上
  • Year and Date: 2011-03-11
• [Presentation] 光標識によるサイトカイニン受容体の結合部位の同定 (2011)
  • Author(s): 安藤和紀、青山卓史、山篠貴史、水野猛、野崎浩、林謙一郎
  • Organizer: 植物化学調節学会第46回大会
  • Place of Presentation: 宇都宮大学、栃木県
• [Presentation] 細胞内オーキシン分布の可視化に関する研究IAAのリアルタイムイメージング (2011)
  • Author(s): 福永紫穂、青山卓史、野崎浩、林謙一郎
  • Organizer: 植物化学調節学会第46回大会
  • Place of Presentation: 宇都宮大学、栃木県
• [Presentation] 細胞の極性を決めるタンパク質間相互作用 (2011)
  • Author(s): 草野博彰、和田悠貴香、安斎尚子、島田浩章、松井南、青山卓史
  • Organizer: 第24回植物脂質シンポジウム
  • Place of Presentation: 東京大学、東京都
• [Presentation] 光標識によるサイトカイニン受容体の結合部位の同定 (2011)
  • Author(s): 安藤和紀, ら
  • Organizer: 植物化学調節学会第46回大会
  • Place of Presentation: 宇都宮大学
• [Presentation] 細胞の極性を決めるタンパク質問相互作用 (2011)
  • Author(s): 草野博彰, ら
  • Organizer: 第24回植物脂質シンポジウム
  • Place of Presentation: 東京大学
• [Presentation] ケージドサイトカイニンの設計と生理活性 (2010)
  • Author(s): 安藤和紀, 日下直之, 青山卓史, 野崎浩, 林謙一郎
  • Organizer: 植物化学調節学会第45回大会
  • Place of Presentation: 神戸大学、兵庫県
  • Year and Date: 2010-11-05
• [Presentation] ケージドサイトカイニンの設計と生理活性 (2010)
  • Author(s): 安藤和紀, ら
  • Organizer: 植物化学調節学会第45回大会
  • Place of Presentation: 神戸大学
  • Year and Date: 2010-11-05
• [Presentation] Regulatory mechanism for shaping plant cells (2010)
  • Author(s): Aoyama, T.
  • Organizer: International Symposium on Young Researcher Global Leader Training Program Shizuoka University
  • Place of Presentation: Hamamatsu, Japan(招待講演)
  • Year and Date: 2010-10-07
• [Presentation] Regulatory mechanism for shaping plant cells. (2010)
  • Author(s): Aoyama, T., et al.
  • Organizer: International Symposium on "Young Researcher Global Leader Training Program" Shizuoka University
  • Place of Presentation: Hamamatsu, Japan
  • Year and Date: 2010-10-07
• [Presentation] 環境刺激に応答したPIP5K遺伝子の機能 (2010)
  • Author(s): 和田悠貴香、草野博彰、安田敬子、柘植知彦、青山卓史
  • Organizer: 第23回植物脂質シンポジウム
  • Place of Presentation: 京都大学、京都府
• [Presentation] シロイヌナズナ・ホスホリパーゼD. 1遺伝子の遺伝学的解析 (2010)
  • Author(s): 安斎尚子、大橋洋平、谷口雅俊、柘植知彦、青山卓史
  • Organizer: 第23回植物脂質シンポジウム
  • Place of Presentation: 京都大学、京都府
• [Presentation] 植物細胞の形態形成における位置情報のシグナル (2010)
  • Author(s): 草野博彰、和田悠貴香、安斎尚子、島田浩章、松井南、青山卓史
  • Organizer: 第23回植物脂質シンポジウム
  • Place of Presentation: 京都大学、京都府
• [Presentation] Genetic analysis of an Arabidopsis taliana PX-PH-type phospholipase D gene PLDzeta1 (2010)
  • Author(s): Naoko Anzai, Yohei Ohashi, Masatoshi Taniguchi, Tomohiko Tsuge, and Takashi Aoyama
  • Organizer: From Plant Biology to Crop Biotechnology
  • Place of Presentation: Suzhou China
• [Presentation] Regulatory mechanism for shaping plant cells (2010)
  • Author(s): Takashi Aoyama, Yukika Wada, Naoko Anzai, and Hiroaki Kusano
  • Organizer: International Symposium on Young Researcher Global Leader Training Program, Shizuoka University
  • Place of Presentation: Hamamatsu Japan
• [Presentation] Function of PIP5K genes in the root hair elongation responsive to environmental stimuli (2010)
  • Author(s): Yukika Wada, Hiroaki Kusano, Keiko Yasuda, Tomohiko Tsuge, and Takashi Aoyama
  • Organizer: Function of PIP5K genes in the root hair elongation responsive to environmental stimuli
  • Place of Presentation: Cairns, Australia
• [Presentation] 環境刺激に応答した根毛伸長におけるPIP5K遺伝子の機能 (2010)
  • Author(s): 和田悠貴香、草野博彰、安田敬子、柘植知彦、青山卓史
  • Organizer: 日本植物生理学会・2010年度年会
  • Place of Presentation: 熊本大学、熊本県
• [Presentation] 植物細胞の形態形成における位置情報のシグナル (2010)
  • Author(s): 草野博彰, ら
  • Organizer: 第23回植物脂質シンポジウム
  • Place of Presentation: 京都大学
• [Presentation] 植物細胞の極性制御に関わるリン脂質シグナル (2009)
  • Author(s): 青山卓史
  • Organizer: 静岡大学・若手グローバル研究リーダー育成プログラムシンポジウム
  • Place of Presentation: 静岡大学、静岡県
  • Year and Date: 2009-11-11
• [Presentation] Biological function of the Arabidopsis PX-PH-type phospholipase Ds (2009)
  • Author(s): Yukimi Y. Taniguchi, Masatoshi Taniguchi, Naoko Anzai, Yukika Wada, Yohei Ohashi, Tomohiko Tsuge, and Takashi Aoyama
  • Organizer: The 3rd Asian Symposium on Plant Lipids and The 22nd Japanese Symposium on Plant Lipids
  • Place of Presentation: Yokohama, Japan
• [Book] Phospholipid signaling in root hair development. in "Root Hairs, Excellent Tools for the Study of Plant Molecular Cell Biology" (2009)
  • Author(s): Aoyama, T
  • Publisher: Springer
• [Remarks]
  • URL: http://rdb.kuicr.kyoto-u.ac.jp/researchers/view/aoyama+takashi
• [Remarks]
  • URL: http://rdb.kuicr.kyoto-u.ac.jp/researchers/view/aoyama+takashi
• [Remarks]
  • URL: http://rdb.kuicr.kyoto-u.ac.jp/researchers/view/aoyama+takashi