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Study on metabolic system and function of cis-zeatin in monocots

Research Project

Project/Area Number 21370023
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SAKAKIBARA Hitoshi  独立行政法人理化学研究所, 生産機能研究グループ, グループディレクター (20242852)

Research Collaborator KUDO Toru  独立行政法人理化学研究所, 生産機能研究グループ, 特別研究員
KOJIMA Mikiko  独立行政法人理化学研究所, 生産機能研究グループ, 技師
MAKITA Nobue  独立行政法人理化学研究所, 生産機能研究グループ, テクニカルスタッフ
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywordsイネ / サイトカイニン / 配糖化 / 受容体 / 生長制御
Research Abstract

We have demonstrated that cZ inhibits seminal root elongation and to up-regulates cytokinin-inducible genes, and its activities were comparable to those of tZ. We identified three rice cZ-O-glucosyltransferases and found that their overexpressors exhibited short shoot phenotypes, delay of leaf senescence, and decrease in crown root number. These results strongly suggest that cZ activity has a physiological impact on growth and development in rice. We demonstrated that a part of cytokinin receptors are localized in endoplasmic reticulum membrane.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (34 results)

All 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results) Presentation (12 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Cytokinin activity of cis-zeatin and phenotypic alterations induced by over-expression of cis-zeatin-O-glucosyltransferase in rice2012

    • Author(s)
      Kudo, T., Makita, N., Kojima, M. and Sakakibara, H.
    • Journal Title

      Plant Physiol

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis lonely guy(LOG) multiple mutants reveal a central role of the LOG-dependent pathway in cytokinin activation2012

    • Author(s)
      Tokunaga, H., Kojima, M., Kuroha, T., Ishida, T., Sugimoto, K., Kiba, T. and Sakakibara, H.
    • Journal Title

      Plant J

      Volume: 69 Pages: 355-365

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis lonely guy (LOG) multiple mutants reveal a central role of the LOG-dependent pathway in cytokinin activation2012

    • Author(s)
      Tokunaga,H., Kojima, M., Kuroha, T., Ishida, T., Sugimoto, K., Kiba, T. and Sakakibara, H.
    • Journal Title

      Plant Journal

      Volume: 69 Issue: 2 Pages: 355-365

    • DOI

      10.1111/j.1365-313x.2011.04795.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] N-Glucosyltransferase UGT76C2 is involved in cytokinin homeostasis and cytokinin response in Arabidopsis thaliana2011

    • Author(s)
      Wang, J., Ma, X.-M., Kojima, M., Sakakibara, H. and Hou, B.-K.
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Pages: 2200-2213

    • NAID

      10030306688

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ligand-binding properties and subcellular localization of maize cytokinin receptors2011

    • Author(s)
      Lomin, S. N., Yonekura-Sakakibara, K., Romanov, G. A. and Sakakibara, H.
    • Journal Title

      J. Exp. Bot

      Volume: 62 Pages: 5149-5159

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hormonal control of nitrogen acquisition : Roles of auxin, abscisic acid and cytokinin2011

    • Author(s)
      Kiba, T., Kudo, T., Kojima, M. and Sakakibara, H.
    • Journal Title

      J. Exp. Bot

      Volume: 62 Pages: 1399-1409

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ligand-binding properties and subcellular localization of maize cytokinin receptors2011

    • Author(s)
      Lomin, S. N., Yonekura-Sakakibara, K., Romanov, G. A. and Sakakibara, H.
    • Journal Title

      Journal of Experimental Botany

      Volume: 62 Issue: 14 Pages: 5149-5159

    • DOI

      10.1093/jxb/err220

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] N-Glucosyltransferase UGT76C2 is involved in cytokinin homeostasis and cytokinin response in Arabidopsis thaliana2011

    • Author(s)
      Wang, J., Ma, X.-M., Kojima, M., Sakakibara, H., Hou, B.-K.
    • Journal Title

      Plant Cell Physiology

      Volume: 52 Issue: 12 Pages: 2200-2213

    • DOI

      10.1093/pcp/pcr152

    • NAID

      10030306688

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hormonal control of nitrogen acquisition : Roles of auxin, abscisic acid and cytokinin2011

    • Author(s)
      Takatoshi Kiba
    • Journal Title

      Journal of Experimental Botany

      Volume: 62 Pages: 1399-1409

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolism and long-distance translocation of cytokinin2010

    • Author(s)
      Kudo, T., Kiba, T. and Sakakibara, H
    • Journal Title

      J. Integr. Plant Biol

      Volume: 52 Pages: 53-60

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of cytokinin in the regulation of plant development2010

    • Author(s)
      Takatoshi Kiba
    • Journal Title

      Plant Developmental Biology-Biotechnological Perspectives

      Volume: 2 Pages: 237-254

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Metabolism and long-distance translocation of cytokinin2010

    • Author(s)
      Toru Kudo
    • Journal Title

      Journal of Integrative Plant Biology 52

      Pages: 53-60

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis for cytokinin biosynthesis2009

    • Author(s)
      Kamada-Nobusada, T. and Sakakibara, H.
    • Journal Title

      Phytochemistry

      Volume: 70 Pages: 444-449

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analyses of LONELY GUY cytokinin-activating enzymes reveal the importance of the direct activation pathway in Arabidopsis2009

    • Author(s)
      Kuroha, T., Tokunaga, H., Kojima, M., Ueda, N., Ishida, T., Nagawa, S., Fukuda, H., Sugimoto, K. and Sakakibara, H.
    • Journal Title

      Plant Cell

      Volume: 21 Pages: 3152-3169

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Highly-sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry : an application for hormone profiling in Oryza sativa2009

    • Author(s)
      Kojima, M., Kamada-Nobusada, T., Komatsu, H., Takei, K., Kuroha, T., Mizutani, M., Ashikari, M., Ueguchi-Tanaka, M., Matsuoka, M., Suzuki, K. and Sakakibara, H.
    • Journal Title

      Plant Cell Physiol

      Volume: 60 Pages: 1201-1214

    • NAID

      10027341243

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] High-throughput and high-sensitive analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry : an application for hormone profiling in Oryza sativa2009

    • Author(s)
      Mikiko Kojima
    • Journal Title

      Plant Cell Physiology 50

      Pages: 1201-1214

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analyses of LONELY GUY cytokinin-activating enzymes reveal the importance of the direct activation pathway in Arabidopsis2009

    • Author(s)
      Takeshi Kuroha
    • Journal Title

      Plant Cell 21

      Pages: 3152-3169

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネにおけるシスゼアチンのサイトカイニン活性2011

    • Author(s)
      工藤徹、槇田庸絵、小嶋美紀子、榊原均
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-23
    • Related Report
      2011 Final Research Report
  • [Presentation] イネにおけるシスゼアチンのサイトカイニン活性2011

    • Author(s)
      工藤徹、槇田庸絵、小嶋美紀子、榊原均
    • Organizer
      日本生化学会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structural variation of cytokinin and its biological importance2010

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      International Symposium on Plant Productivity
    • Place of Presentation
      Peterborough, Canada
    • Year and Date
      2010-10-26
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Nitrogendependent regulation of cytokinin metabolism2010

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      Nitrogen 2010
    • Place of Presentation
      Inuyama, Japan
    • Year and Date
      2010-07-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Nitrogen-dependent regulation of cytokinin metabolism2010

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      Nitrogen 2010
    • Place of Presentation
      Inuyama, Japan
    • Year and Date
      2010-07-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Regulation of cytokinin action in plants : Biological importance of side-chain variation2010

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      20th International Conference on Plant Growth Substances
    • Place of Presentation
      Tarragona, Spain
    • Year and Date
      2010-06-28
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Cytokinin biosynthesis pathway and its regulation : How do plants regulate their growth and development by cytokinin actions2009

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      International Conference on Biological Science Faculty of Biology Universitas Gadjah Mada 2009
    • Place of Presentation
      Yog Yakarta, Indonesia
    • Year and Date
      2009-10-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Cytokinin biosynthesis pathway and its regulation : How do plants regulate their growth and development by cytokinin actions2009

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      International Conference on Biological Science Faculty of Biology Universitas Gadjah Mada 2009
    • Place of Presentation
      Indonesia, Yog Yakarta
    • Year and Date
      2009-10-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cytokinin biosynthesis pathway : Not as simple as it looks2009

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      Auxins and Cytokinins Plant Development 3^<rd> International Symposium
    • Place of Presentation
      Plague, Czech
    • Year and Date
      2009-07-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Cytokinin biosynthesis pathway : Not as simple as it looks2009

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      Auxins and Cytokinins Plant Development 3^<rd> International Symposium
    • Place of Presentation
      Czech, Plague
    • Year and Date
      2009-07-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Regulation of cytokinin biosynthesis integrating root and shoot responses to nitrogen supply2009

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      Plant Vascular Biology and Agriculture 2009
    • Place of Presentation
      Chongqing, China
    • Year and Date
      2009-06-22
    • Related Report
      2011 Final Research Report
  • [Presentation] Regulation of cytokinin biosynthesis integrating root and shoot responses to nitrogen supply2009

    • Author(s)
      Hitoshi Sakakibara
    • Organizer
      Plant Vascular Biology and Agriculture 2009
    • Place of Presentation
      China, Chongqing
    • Year and Date
      2009-06-22
    • Related Report
      2009 Annual Research Report
  • [Book] Role of cytokinin in the regulation of plant development. Plant Developmental Biology. Biotechnological Perspectives E-C. Pua and M. R. Davey(eds.)2010

    • Author(s)
      Kiba, T. and Sakakibara, H.
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://labs.psc.riken.jp/brt/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://labs.psc.riken.jp/brt/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://labs.psc.riken.jp/brt/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://labs.psc.riken.jp/brt/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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