Study on metabolic system and function of cis-zeatin in monocots

• Project/Area Number: 21370023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SAKAKIBARA Hitoshi 独立行政法人理化学研究所, 生産機能研究グループ, グループディレクター (20242852)
• Research Collaborator: KUDO Toru 独立行政法人理化学研究所, 生産機能研究グループ, 特別研究員 ; KOJIMA Mikiko 独立行政法人理化学研究所, 生産機能研究グループ, 技師 ; MAKITA Nobue 独立行政法人理化学研究所, 生産機能研究グループ, テクニカルスタッフ
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: イネ / サイトカイニン / 配糖化 / 受容体 / 生長制御

Research Abstract

We have demonstrated that cZ inhibits seminal root elongation and to up-regulates cytokinin-inducible genes, and its activities were comparable to those of tZ. We identified three rice cZ-O-glucosyltransferases and found that their overexpressors exhibited short shoot phenotypes, delay of leaf senescence, and decrease in crown root number. These results strongly suggest that cZ activity has a physiological impact on growth and development in rice. We demonstrated that a part of cytokinin receptors are localized in endoplasmic reticulum membrane.

Research Products

• [Journal Article] Cytokinin activity of cis-zeatin and phenotypic alterations induced by over-expression of cis-zeatin-O-glucosyltransferase in rice (2012)
  • Author(s): Kudo, T., Makita, N., Kojima, M. and Sakakibara, H.
  • Journal Title: Plant Physiol
  • Peer Reviewed
• [Journal Article] Arabidopsis lonely guy(LOG) multiple mutants reveal a central role of the LOG-dependent pathway in cytokinin activation (2012)
  • Author(s): Tokunaga, H., Kojima, M., Kuroha, T., Ishida, T., Sugimoto, K., Kiba, T. and Sakakibara, H.
  • Journal Title: Plant J Volume: 69 Pages: 355-365
  • Peer Reviewed
• [Journal Article] Arabidopsis lonely guy (LOG) multiple mutants reveal a central role of the LOG-dependent pathway in cytokinin activation (2012)
  • Author(s): Tokunaga,H., Kojima, M., Kuroha, T., Ishida, T., Sugimoto, K., Kiba, T. and Sakakibara, H.
  • Journal Title: Plant Journal Volume: 69 Issue: 2 Pages: 355-365
  • DOI: 10.1111/j.1365-313x.2011.04795.x
  • Peer Reviewed
• [Journal Article] N-Glucosyltransferase UGT76C2 is involved in cytokinin homeostasis and cytokinin response in Arabidopsis thaliana (2011)
  • Author(s): Wang, J., Ma, X.-M., Kojima, M., Sakakibara, H. and Hou, B.-K.
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 2200-2213
  • NAID: 10030306688
  • Peer Reviewed
• [Journal Article] Ligand-binding properties and subcellular localization of maize cytokinin receptors (2011)
  • Author(s): Lomin, S. N., Yonekura-Sakakibara, K., Romanov, G. A. and Sakakibara, H.
  • Journal Title: J. Exp. Bot Volume: 62 Pages: 5149-5159
  • Peer Reviewed
• [Journal Article] Hormonal control of nitrogen acquisition : Roles of auxin, abscisic acid and cytokinin (2011)
  • Author(s): Kiba, T., Kudo, T., Kojima, M. and Sakakibara, H.
  • Journal Title: J. Exp. Bot Volume: 62 Pages: 1399-1409
  • Peer Reviewed
• [Journal Article] Ligand-binding properties and subcellular localization of maize cytokinin receptors (2011)
  • Author(s): Lomin, S. N., Yonekura-Sakakibara, K., Romanov, G. A. and Sakakibara, H.
  • Journal Title: Journal of Experimental Botany Volume: 62 Issue: 14 Pages: 5149-5159
  • DOI: 10.1093/jxb/err220
  • Peer Reviewed
• [Journal Article] N-Glucosyltransferase UGT76C2 is involved in cytokinin homeostasis and cytokinin response in Arabidopsis thaliana (2011)
  • Author(s): Wang, J., Ma, X.-M., Kojima, M., Sakakibara, H., Hou, B.-K.
  • Journal Title: Plant Cell Physiology Volume: 52 Issue: 12 Pages: 2200-2213
  • DOI: 10.1093/pcp/pcr152
  • NAID: 10030306688
  • Peer Reviewed
• [Journal Article] Hormonal control of nitrogen acquisition : Roles of auxin, abscisic acid and cytokinin (2011)
  • Author(s): Takatoshi Kiba
  • Journal Title: Journal of Experimental Botany Volume: 62 Pages: 1399-1409
  • Peer Reviewed
• [Journal Article] Metabolism and long-distance translocation of cytokinin (2010)
  • Author(s): Kudo, T., Kiba, T. and Sakakibara, H
  • Journal Title: J. Integr. Plant Biol Volume: 52 Pages: 53-60
  • Peer Reviewed
• [Journal Article] Role of cytokinin in the regulation of plant development (2010)
  • Author(s): Takatoshi Kiba
  • Journal Title: Plant Developmental Biology-Biotechnological Perspectives Volume: 2 Pages: 237-254
• [Journal Article] Metabolism and long-distance translocation of cytokinin (2010)
  • Author(s): Toru Kudo
  • Journal Title: Journal of Integrative Plant Biology 52 Pages: 53-60
  • Peer Reviewed
• [Journal Article] Molecular basis for cytokinin biosynthesis (2009)
  • Author(s): Kamada-Nobusada, T. and Sakakibara, H.
  • Journal Title: Phytochemistry Volume: 70 Pages: 444-449
  • Peer Reviewed
• [Journal Article] Functional analyses of LONELY GUY cytokinin-activating enzymes reveal the importance of the direct activation pathway in Arabidopsis (2009)
  • Author(s): Kuroha, T., Tokunaga, H., Kojima, M., Ueda, N., Ishida, T., Nagawa, S., Fukuda, H., Sugimoto, K. and Sakakibara, H.
  • Journal Title: Plant Cell Volume: 21 Pages: 3152-3169
  • Peer Reviewed
• [Journal Article] Highly-sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry : an application for hormone profiling in Oryza sativa (2009)
  • Author(s): Kojima, M., Kamada-Nobusada, T., Komatsu, H., Takei, K., Kuroha, T., Mizutani, M., Ashikari, M., Ueguchi-Tanaka, M., Matsuoka, M., Suzuki, K. and Sakakibara, H.
  • Journal Title: Plant Cell Physiol Volume: 60 Pages: 1201-1214
  • NAID: 10027341243
  • Peer Reviewed
• [Journal Article] High-throughput and high-sensitive analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry : an application for hormone profiling in Oryza sativa (2009)
  • Author(s): Mikiko Kojima
  • Journal Title: Plant Cell Physiology 50 Pages: 1201-1214
  • Peer Reviewed
• [Journal Article] Functional analyses of LONELY GUY cytokinin-activating enzymes reveal the importance of the direct activation pathway in Arabidopsis (2009)
  • Author(s): Takeshi Kuroha
  • Journal Title: Plant Cell 21 Pages: 3152-3169
  • Peer Reviewed
• [Presentation] イネにおけるシスゼアチンのサイトカイニン活性 (2011)
  • Author(s): 工藤徹、槇田庸絵、小嶋美紀子、榊原均
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-23
• [Presentation] イネにおけるシスゼアチンのサイトカイニン活性 (2011)
  • Author(s): 工藤徹、槇田庸絵、小嶋美紀子、榊原均
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2011-09-23
• [Presentation] Structural variation of cytokinin and its biological importance (2010)
  • Author(s): Hitoshi Sakakibara
  • Organizer: International Symposium on Plant Productivity
  • Place of Presentation: Peterborough, Canada
  • Year and Date: 2010-10-26
• [Presentation] Nitrogendependent regulation of cytokinin metabolism (2010)
  • Author(s): Hitoshi Sakakibara
  • Organizer: Nitrogen 2010
  • Place of Presentation: Inuyama, Japan
  • Year and Date: 2010-07-28
• [Presentation] Nitrogen-dependent regulation of cytokinin metabolism (2010)
  • Author(s): Hitoshi Sakakibara
  • Organizer: Nitrogen 2010
  • Place of Presentation: Inuyama, Japan
  • Year and Date: 2010-07-28
• [Presentation] Regulation of cytokinin action in plants : Biological importance of side-chain variation (2010)
  • Author(s): Hitoshi Sakakibara
  • Organizer: 20th International Conference on Plant Growth Substances
  • Place of Presentation: Tarragona, Spain
  • Year and Date: 2010-06-28
• [Presentation] Cytokinin biosynthesis pathway and its regulation : How do plants regulate their growth and development by cytokinin actions (2009)
  • Author(s): Hitoshi Sakakibara
  • Organizer: International Conference on Biological Science Faculty of Biology Universitas Gadjah Mada 2009
  • Place of Presentation: Yog Yakarta, Indonesia
  • Year and Date: 2009-10-17
• [Presentation] Cytokinin biosynthesis pathway and its regulation : How do plants regulate their growth and development by cytokinin actions (2009)
  • Author(s): Hitoshi Sakakibara
  • Organizer: International Conference on Biological Science Faculty of Biology Universitas Gadjah Mada 2009
  • Place of Presentation: Indonesia, Yog Yakarta
  • Year and Date: 2009-10-17
• [Presentation] Cytokinin biosynthesis pathway : Not as simple as it looks (2009)
  • Author(s): Hitoshi Sakakibara
  • Organizer: Auxins and Cytokinins Plant Development 3^<rd> International Symposium
  • Place of Presentation: Plague, Czech
  • Year and Date: 2009-07-11
• [Presentation] Cytokinin biosynthesis pathway : Not as simple as it looks (2009)
  • Author(s): Hitoshi Sakakibara
  • Organizer: Auxins and Cytokinins Plant Development 3^<rd> International Symposium
  • Place of Presentation: Czech, Plague
  • Year and Date: 2009-07-11
• [Presentation] Regulation of cytokinin biosynthesis integrating root and shoot responses to nitrogen supply (2009)
  • Author(s): Hitoshi Sakakibara
  • Organizer: Plant Vascular Biology and Agriculture 2009
  • Place of Presentation: Chongqing, China
  • Year and Date: 2009-06-22
• [Presentation] Regulation of cytokinin biosynthesis integrating root and shoot responses to nitrogen supply (2009)
  • Author(s): Hitoshi Sakakibara
  • Organizer: Plant Vascular Biology and Agriculture 2009
  • Place of Presentation: China, Chongqing
  • Year and Date: 2009-06-22
• [Book] Role of cytokinin in the regulation of plant development. Plant Developmental Biology. Biotechnological Perspectives E-C. Pua and M. R. Davey(eds.) (2010)
  • Author(s): Kiba, T. and Sakakibara, H.
• [Remarks]
  • URL: http://labs.psc.riken.jp/brt/
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  • URL: http://labs.psc.riken.jp/brt/