Studies on the role of prostagalandins in the induction of ovulation in vertebrates
| Project/Area Number |
21370024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OGIWARA Katsueki 北海道大学, 大学院・理学研究院, 教授 (00422006)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | メダカ / 生殖 / 排卵 / プロスタグランジン / 細胞・組織 / 動物 |
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| Research Abstract |
In in vitro ovulation system using medaka ovarian preovulatory follicles, we confirmed that prostaglandin E2 (PGE2) played a role in the fish ovulation. Activity levels of cyclooxygenase-2, the enzyme responsible for PGE2 synthesis, and intrafollicular levels of PGE2 were both relatively constant, while the transcript levels of EP4b, a PGE2 receptor subtype, were drastically induced at ovulation, indicating the importance of induction of the receptor for PGE2's effect on medaka ovulation. Transcription of EP4b gene was demonstrated to be regulated by luteininzing hormone and nuclear progesterone receptor.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Kbus/Idr, a Mutant Mouse Strain with Skeletal Abnormalities and Hypophosphatemia : Identification as an Allele of'Hyp'.2011
Author(s)
Moriyama, K., Hanai, A., Mekada, K., Yoshiki, A., Ogiwara, K., Kimura, A., Takahashi, T.
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Journal Title
J. Biomed. Sci.
Volume: 18
Issue: 1
Pages: 60-60
DOI
Related Report
Peer Reviewed
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[Journal Article] Expression of Cyclooxygenase-2 and Prostaglandin Receptor EP4b mRNA in the Ovary of the Medaka Fish, Oryzias latipes : Possible Involvement in Ovulation2011
Author(s)
Fujimori, C., Ogiwara, K., Hagiwara, A., Rajapakse, S., Kimura, A., Takahashi, T.
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Journal Title
Mol. Cell. Endocrinol.
Volume: 332(1-2)
Pages: 67-77
NAID
Related Report
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[Journal Article] Purification and Characterization of a Major Collagenase from Streptomyces parvulus.2009
Author(s)
Sakurai, Y., Inoue, H., Nishii, W., Takahashi, T., Iino, Y., Yamamoto, M., Takahashi K.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 73(1)
Pages: 21-8
NAID
Related Report
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