How plant cells make a mitotic spindle, without involvement of centrosomes?
| Project/Area Number |
21370026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
MURATA Takashi 基礎生物学研究所, 生物進化研究部門, 准教授 (00242024)
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| Co-Investigator(Renkei-kenkyūsha) |
HASEBE Mitsuyasu 基礎生物学研究所, 生物進化研究部門, 教授 (40237996)
NONAKA Shigenori 基礎生物学研究所, 時空間制御研究室, 准教授 (90435529)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
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| Keywords | 微小管 / 紡錘体 / 隔膜形成体 / 植物細胞 / 顕微鏡技術 / 細胞進化 / 細胞分裂 |
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| Research Abstract |
Centrosomes play a role on formation of a mitotic spindle. It has been a mystery how plant cells form a mitotic spindle in the absence of the centrosomes. We proposed that mitotic spindles in plants are formed by branching of existing microtubules. To evaluate the hypothesis, we examined mechanism of spindle formation by analyzing localization of markers of centrosomes and microtubules during cell division of tobacco suspension culture cells. Gamma-tubulin, which localizes in the centrosome and nucleate microtubules there, dispersed in the spindle and moved in the spindle. In phragmoplast formation, new microtubules were elongated from the existing microtubules.
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Report
(4 results)
Research Products
(15 results)
