Neural basis of cognitive processes underlying insect learning

• Project/Area Number: 21370028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Hokkaido University
• Principal Investigator: MIZUNAMI Makoto 北海道大学, 大学院・理学研究院, 教授 (30174030)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 神経行動 / 学習 / 記憶 / 微小脳 / 昆虫 / 記憶想起 / 認知 / コオロギ / オクトパミン / Mizunami-Unoki Model

Research Abstract

We have proposed a model in which conditioned responses of insects requires activation of neurons that represent unconditioned stimulus. We performed experiments to evaluate predictions from this model and to examine neural substrates underlying this model. We found that crickets that received excessive training exhibit conditioned responses even after blockade of synaptic transmission from neurons that represent US, indicating habit formation in insects. We also found that the octopaminergic neurons projecting to the vertical lobe of the mushroom body participate in memory retrieval in cockroaches.

Research Products

• [Journal Article] Pheromone detection by a pheromone emitter : a small, sex pheromone-specific processing system in the female American cockroach (2011)
  • Author(s): Nishino H., Iwasaki M., and Mizunami M.
  • Journal Title: Chem. Senses Volume: 36 Pages: 261-270
  • Peer Reviewed
• [Journal Article] Critical roles of mecamylamine-sensitive mushroom body neurons in insect olfactory learning (2011)
  • Author(s): Watanabe H., Matsumoto S. C. , Nishino H. and Mizunami M.
  • Journal Title: Neurobiol. Learn. Mem Volume: 95 Pages: 1-13
  • NAID: 120002834739
  • Peer Reviewed
• [Journal Article] Complete mapping of glomeruli based on sensory nerve branching pattern in the primary olfactory center of the cockroach Periplaneta americana (2011)
  • Author(s): Watanabe H., et al
  • Journal Title: Neurobiology of Learning and Memory Volume: 95 Pages: 40-921
  • Peer Reviewed
• [Journal Article] Complete mapping of glomeruli based on sensory nerve branching pattern in the primary olfactory center of the ockroach Periplaneta americana (2010)
  • Author(s): Watanabe H., Nishino H., Nishikawa M., Mizunami M. and Yokohari F.
  • Journal Title: J. Comp. Neurol Volume: 518 Issue: 19 Pages: 3907-3930
  • DOI: 10.1002/cne.22452
  • Peer Reviewed
• [Journal Article] Spatial representation of alarm pheromone information in a secondary olfactory center in the ant brain (2010)
  • Author(s): Yamagata N. and Mizunami M
  • Journal Title: Proc. R. Soc. B Volume: 277 Issue: 1693 Pages: 2465-2475
  • DOI: 10.1098/rspb.2010.0366
  • Peer Reviewed
• [Journal Article] Postembryonic development of sexually dimorphic glomeruli and related interneurons in the cockroach Periplaneta americana (2010)
  • Author(s): Nishino H., Yoritsune A., and Mizunami M
  • Journal Title: Neurosci. Lett Volume: 469 Issue: 1 Pages: 60-64
  • DOI: 10.1016/j.neulet.2009.11.044
  • NAID: 120001909639
  • Peer Reviewed
• [Journal Article] Roles of aminergic neurons in formation and recall of associative memory in crickets (2010)
  • Author(s): Mizunami M., Matsumoto Y
  • Journal Title: Frontiers Behav. Neurosci Volume: 4 Pages: 172-172
  • Peer Reviewed
• [Journal Article] Alarm pheromone processing in the ant brain : an evolutionary perspective (2010)
  • Author(s): Mizunami M., Yamagata N. and Nishino H
  • Journal Title: Frontiers Behav. Neurosci Volume: 4 Pages: 28-28
  • Peer Reviewed
• [Journal Article] Complete mapping of glomeruli based on sensory nerve branching pattern in the primary olfactory center of the cock roach Periplaneta americana. (2010)
  • Author(s): Watanabe H., et al.
  • Journal Title: Journal of Comparative Neurology Volume: 518 Pages: 3907-3930
  • Peer Reviewed
• [Journal Article] Spatial representation of alarm pheromone information in asecondary olfactory center in the ant brain (2010)
  • Author(s): Yamagata N., Mizunami M.
  • Journal Title: Proceedings of the Royal Society, Part B. Volume: 277 Pages: 2465-2475
  • Peer Reviewed
• [Journal Article] Postembryonic development of sexually dimorphic glomeruli and related interneurons in the cockroach Periplaneta americana. (2010)
  • Author(s): Nishino N., et al.
  • Journal Title: Neuroscience Letters Volume: 469 Pages: 60-64
  • Peer Reviewed
• [Journal Article] Roles of aminergic neurons in formation and recall of associative memory in crickets (2010)
  • Author(s): Mizunami M., Matsumoto Y
  • Journal Title: Frontiers in Behavioral Neuroscience Volume: 4 Pages: 172-172
  • Peer Reviewed
• [Journal Article] Alarm pheromone processing in the ant brain : an evolutionary perspective (2010)
  • Author(s): Mizunami M., et al.
  • Journal Title: Frontiers in Behavioral Neuroscience Volume: 4 Pages: 28-28
  • Peer Reviewed
• [Journal Article] Differential odor processing in two olfactory pathways in the honeybee (2009)
  • Author(s): Yamagata N., Schmuker M., Szyszka P., Mizunami M. , and Menzel R
  • Journal Title: Frontiers Systems Neurosci Volume: 3 Pages: 16-16
  • DOI: 10.3389/neuro.06.016.2009
  • Peer Reviewed
• [Journal Article] Stimulation of the cAMP system by the nitric oxide-cGMP system underlying the formation of long-term memory in an insect (2009)
  • Author(s): Matsumoto Y., Hatano A., Unoki S. and Mizunami M.
  • Journal Title: Neurosci. Lett Volume: 467 Issue: 2 Pages: 81-85
  • DOI: 10.1016/j.neulet.2009.10.008
  • NAID: 120001702451
  • Peer Reviewed
• [Journal Article] Roles of octopaminergic and dopaminergic neurons in appetitive and aversive memory recall in an insect (2009)
  • Author(s): Mizunami M., Unoki S., Mori Y., Hirashima D., Hatano A., and Matsumoto Y.
  • Journal Title: BMC Biology Volume: 7 Issue: 1 Pages: 46-46
  • DOI: 10.1186/1741-7007-7-46
  • Peer Reviewed
• [Journal Article] Why the carrot is more effective than the stick : Different dynamics of punishment memory and reward memory and its possible biological basis (2009)
  • Author(s): Nakatani Y., Matsumoto Y., Mori Y., Hirashima D., Nishino H., Arikawa K. and Mizunami M.
  • Journal Title: Neurobiol. Learn. Mem Volume: 92 Issue: 3 Pages: 370-380
  • DOI: 10.1016/j.nlm.2009.05.003
  • Peer Reviewed
• [Journal Article] Different growth patterns of two adjacent glomeruli responsible for sex-pheromone processing during postembryonic development of the cockroach Periplaneta americana (2009)
  • Author(s): Nishino H., Yoritsune A., and Mizunami M.
  • Journal Title: Neurosci. Lett Volume: 462 Issue: 3 Pages: 219-224
  • DOI: 10.1016/j.neulet.2009.01.012
  • NAID: 120001538105
  • Peer Reviewed
• [Journal Article] Functional and topographic segregation of glomeruli revealed by local staining of antennal sensory neurons in the honeybee Apis mellifera (2009)
  • Author(s): Nishino H., Nishikawa M., Mizunami M., and Yokohari F.
  • Journal Title: J. Comp. Neurol Volume: 515 Issue: 2 Pages: 161-180
  • DOI: 10.1002/cne.22064
  • Peer Reviewed
• [Journal Article] Systemic RNA interference for the study of learning and memory in an insect (2009)
  • Author(s): Takahashi T., Hamada A., Miyawaki K., Matsumoto Y., Mito T., Noji S., and Mizunami M.
  • Journal Title: J. Neurosci. Methods Volume: 179 Pages: 9-15
  • Peer Reviewed
• [Journal Article] Differential odor processing in two olfactory pathways in the honeybee. (2009)
  • Author(s): Yamagata N., et al.
  • Journal Title: Frontiers Systems Neurosci. 3 Pages: 16-16
  • Peer Reviewed
• [Journal Article] Stimulation of the cAMP system by the nitric oxide-cGMP system underlying the formation of long-term memory in an insect. (2009)
  • Author(s): Matsumoto Y., et al.
  • Journal Title: Neurosci.Lett. 467 Pages: 81-85
  • Peer Reviewed
• [Journal Article] Roles of octopaminergic and dopaminergic neurons in appetitive and aversive memory recall in an insect. (2009)
  • Author(s): Mizunami M., et al.
  • Journal Title: BMC Biology 7 Pages: 46-46
  • Peer Reviewed
• [Journal Article] Why the carrot is more effective than the stick : Different dynamics of punishment memory and reward memory and its possible biological basis. (2009)
  • Author(s): Nakatani Y., et al.
  • Journal Title: Neurobiol.Learn.Mem. 92 Pages: 370-380
  • Peer Reviewed
• [Journal Article] Different growth patterns of two adjacent glomeruli responsible for sex-pheromone processing during postembryonic development of the cockroach Periplaneta americana. (2009)
  • Author(s): Nishino H., et al.
  • Journal Title: Neurosci.Lett. 462 Pages: 219-224
  • Peer Reviewed
• [Presentation] コオロギの嗅覚連合学習に対するカフェインの影響 (2011)
  • Author(s): 杉町誓児、吉富絵美、松本幸久、水波誠、岡田二郎
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京大学、東京
  • Year and Date: 2011-12-01
• [Presentation] コオロギの嗅覚連合学習に対するカフェインの影響 (2011)
  • Author(s): 杉町誓児, 他4名
  • Organizer: 日本環境ホルモン学会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2011-12-01
• [Presentation] 昆虫微小脳の機能的設計に関する研究(動物学会賞受賞者講演) (2011)
  • Author(s): 水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 大雪クリスタルホール、旭川
  • Year and Date: 2011-09-22
• [Presentation] ワモンゴキブリのキノコ体における視覚と嗅覚情報の入力部位の比較 (2011)
  • Author(s): 岩崎正純、水波誠、西野浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 大雪クリスタルホール、旭川
  • Year and Date: 2011-09-22
• [Presentation] 昆虫微小脳の機能的設計に関する研究 (2011)
  • Author(s): 水波誠
  • Organizer: 日本動物学会、動物学会賞受賞者講
  • Place of Presentation: 大雪クリスタルホール(旭川市)(招待講演)
  • Year and Date: 2011-09-22
• [Presentation] フタホシコオロギの嗅覚長期記憶のエピジェネティック制御 (2011)
  • Author(s): 松本幸久, 他2名
  • Organizer: 日本動物学会
  • Place of Presentation: 大雪アリーナ(旭川市)
  • Year and Date: 2011-09-22
• [Presentation] ワモンゴキブリのキノコにおける視覚と嗅覚情報の入力部位の化較 (2011)
  • Author(s): 岩崎正純, 他2名
  • Organizer: 日本動物学会
  • Place of Presentation: 大雪アリーナ(旭川市)
  • Year and Date: 2011-09-22
• [Presentation] フタホシコオロギの嗅覚長期記憶のエピジェネティック制御 (2011)
  • Author(s): 松本幸久、佐藤千尋、水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 大雪クリスタルホール、旭川
  • Year and Date: 2011-09-21
• [Presentation] Habit formation by extended Pavlovian training in crickets (2011)
  • Author(s): Mizunami M, Hatano A, Arai R, Matsumoto Y.
  • Organizer: 8th ICCPB
  • Place of Presentation: Nagoya convention center, Nagoya
  • Year and Date: 2011-06-01
• [Presentation] Sensory preconditioning in the cricket Gryllus bimaculatus : Associations between neutral sensory stimuli (2011)
  • Author(s): Matsumoto Y, Hirashima D, Mizunami M.
  • Organizer: 8th ICCPB
  • Place of Presentation: Nagoya convention center, Nagoya
  • Year and Date: 2011-06-01
• [Presentation] Detection of female sex pheromone by male and female cockroaches : a comparative study (2011)
  • Author(s): Nishino H, Iwasaki M, Mizunami M.
  • Organizer: 8th ICCPB
  • Place of Presentation: Nagoya convention center, Nagoya
  • Year and Date: 2011-06-01
• [Presentation] Habit formation by extended Pavlovian training in crickets (2011)
  • Author(s): Mizunami M., et al
  • Organizer: 8th International Conference for Comparative Physiology and Biochemistry
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-06-01
• [Presentation] Sensory preconditioning in the cricket Gryllus bimaculatus : Associations between neutral sensory stimuli (2011)
  • Author(s): Matsumoto Y., et al
  • Organizer: Comparative Physiology and Biochemistry
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-06-01
• [Presentation] a comparative study (2011)
  • Author(s): Nishino H., et al
  • Organizer: 8th International Conference for Comparative Physiology and Biochemistry
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-06-01
• [Presentation] フタホシコオロギの加齢性記憶障害における-酸化窒素の役割 (2010)
  • Author(s): 松本幸久、佐藤千尋、水波誠
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2010-09-03
• [Presentation] フタホシコオロギの加齢性記憶障害における一酸化窒素の役割 (2010)
  • Author(s): 松本幸久
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] Age-related memory impairment and its pharmacological rescue in the cricket (2010)
  • Author(s): Matsumoto Y, Matsumoto S. C., Takahashi T, and Mizunami M
  • Organizer: 9th International congress of Neuroethology
  • Place of Presentation: Salamanca University, Salamanaca, Spain
  • Year and Date: 2010-08-05
• [Presentation] Effect of epinastine on acquisition and retrieval of appetitive olfactory memory in the cockroach (2010)
  • Author(s): Matsumoto S.C, Matsumoto Y, and Mizunami M
  • Organizer: 9th International congress of Neuroethology
  • Place of Presentation: Salamanca University, Salamanaca, Spain
  • Year and Date: 2010-08-05
• [Presentation] Cognitive aspects of insect classical conditioning revealed by studying the roles of aminergic neurons in olfactory and visual memory recall (2010)
  • Author(s): Mizunami M, Unoki S, Matsumoto Y
  • Organizer: 9th International congress of Neuroethology
  • Place of Presentation: Salamanca University, Salamanaca, Spain
  • Year and Date: 2010-08-05
• [Presentation] Age-related memory impairment and its pharmacological rescue in the cricket (2010)
  • Author(s): Matsumoto Y.
  • Organizer: 9^<th> International congress of Neuroethology
  • Place of Presentation: Salamanaca, Spain
  • Year and Date: 2010-08-05
• [Presentation] Effect of epinastine on acquisition and retrieval of appetitive olfactory memory in the cockroach (2010)
  • Author(s): Matsumoto S.-C.
  • Organizer: 9^<th> International congress of Neuroethology
  • Place of Presentation: Salamanaca, Spain
  • Year and Date: 2010-08-05
• [Presentation] Cognitive aspects of insect classical conditioning revealed by studying the roles of aminergic neurons in olfactory and visual memory recall (2010)
  • Author(s): Mizunami M.
  • Organizer: 9^<th> International congress of Neuroethology
  • Place of Presentation: Salamanaca, Spain
  • Year and Date: 2010-08-05
• [Presentation] コオロギの匂い学習・記憶におけるアセチルコリン受容体の働き (2010)
  • Author(s): 松本幸久、佐藤千尋、水波誠
  • Organizer: 日本比較生理生化学会
  • Place of Presentation: 九州産業大学、福岡
  • Year and Date: 2010-07-17
• [Presentation] コオロギの匂い学習・記憶におけるアセチルコリン受容体の働き (2010)
  • Author(s): 松本幸久
  • Organizer: 日本比較生理生化学会
  • Place of Presentation: 福岡
  • Year and Date: 2010-07-17
• [Presentation] フタホシコオロギにおける連合条件付けの訓練間隔と記憶の関係 (2009)
  • Author(s): 松本幸久、水波誠
  • Organizer: 日本比較生理生化学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-23
• [Presentation] What can we learn from them? Satellite Symposium on Biomimetic Surface Engineering (2009)
  • Author(s): Mizunami M.
  • Organizer: Biomimetic Materials Design based on Biodiversity Inventory
  • Place of Presentation: Sapporo
  • Year and Date: 2009-10-03
• [Presentation] Insect "microbrains" : What can we learn from them? (2009)
  • Author(s): Mizunami M.
  • Organizer: Biomimetic Materials Design Based on Biodiversity Inventory
  • Place of Presentation: Sapporo
  • Year and Date: 2009-10-03
• [Presentation] 微小脳研究から脳進化の解明へ、成茂シンポジウム (2009)
  • Author(s): 水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-27
• [Presentation] なぜ罰記憶は報酬記憶より忘れやすいのか (2009)
  • Author(s): 水波誠、仲谷嘉洋、松本幸久
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-27
• [Presentation] ゴキブリ触角葉における匂い刺激応答の光学計測 (2009)
  • Author(s): 松本幸久、山方恒宏、佐藤千尋、河野芳明、水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-27
• [Presentation] 微小脳研究から脳進化の解明へ (2009)
  • Author(s): 水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-27
• [Presentation] ゴキブリ触角葉における匂い刺激応答の光学計測 (2009)
  • Author(s): 松本幸久, 他4名
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-27
• [Presentation] なぜ罰記憶は報酬記憶より忘れやすいのか? (2009)
  • Author(s): 水波誠, 他2名
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-27
• [Presentation] Habit formation in insect classical conditioning (2009)
  • Author(s): Mizunami M.
  • Organizer: International Symposium of Life Science
  • Place of Presentation: Fukuoka University, Fukuoka
  • Year and Date: 2009-03-22
• [Presentation] Habit formation in insect classical conditioning. (2009)
  • Author(s): Mizunami M.
  • Organizer: International Symposium of Life Science
  • Place of Presentation: Fukuoka, Japan 招待講演
  • Year and Date: 2009-03-22
• [Presentation] Cognitive process underlying insect classical conditioning (2009)
  • Author(s): Mizunami M.
  • Organizer: Insect Genome Meeting
  • Place of Presentation: Riken, Kobe (invited)
  • Year and Date: 2009-03-11
• [Book] 動物は何を考えているのか?:学習と記憶の比較生物学 (2009)
  • Author(s): 水波誠、松本幸久
• [Book] 動物は何を考えているのか?:学習と記憶の比較生物学 (2009)
  • Author(s): 水波誠、松本幸久
  • Total Pages: 16
  • Publisher: 共立出版
• [Remarks] 報道関連情報(1)北海道新聞に記事、「雌同士恋のライバル認知?ゴキブリの神経反応解析」2010年1月23日
• [Remarks] (2)北海道新聞に記事、「コオロギの脳内の仕組みを解明した北大大学院教授水波誠さん」、ひと2009年、2009年8月14日
• [Remarks] (3)科学新聞、コオロギの記憶読み出しにニューロンの活性化必要、2009年8月14日
• [Remarks] (4)北海道新聞に記事、「コオロギも考えています」、2009年8月5日
• [Remarks] (5)読売新聞に記事、「コオロギの記憶、高度な仕組み」、2009年8月5日
• [Remarks] (6)HBCテレビで報道、「コオロギの記憶を解明」、The NEWS北海道、2009年8月5日
• [Remarks] アウトリーチ活動(1)北海道生物教育会第1回研究協議会にて研修会『比較神経解剖学～コオロギとヒヨコから研究課題を発見する』講師、20116月18日
• [Remarks] 学術上の受賞「昆虫微小脳の機能的設計に関する研究」にて、日本動物学会賞を受賞。2011年9月22日
• [Remarks] ホームページ情報
  • URL: https://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/
• [Remarks]
  • URL: https://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/
• [Remarks]
  • URL: https://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/
• [Remarks]
  • URL: https://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/