Structural biology of protein-protein interactions involved in DNA replication under DNA damage
Project/Area Number |
21370048
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Yokohama City University |
Principal Investigator |
SATO Mamoru 横浜市立大学, 生命ナノシステム科学研究科, 教授 (60170784)
|
Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Hiroshi 横浜市立大学, 生命ナノシステム科学研究科, 助教 (40336590)
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Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
|
Keywords | X線結晶解析 / DNA複製 / DNA損傷応答 / PCNA / ユビキチン化 / 損傷乗り越えDNA合成 / DNAクランプ / 構造解析 |
Research Abstract |
DNA clamp is involved in various cellular events on DNA including DNA replication, repair and replication, and functions as a scaffold protein in those events. DNA clamp forms a ring-shaped trimer that encircles the DNA double helix and slides freely along the DNA axis. PCNA and RAD9/HUS1/RAD1(9-1-1) are DNA clamp in eukaryote. PCNA is a homo-trimer, whereas 9-1-1 is a hetero-trimer. Each subunit of 9-1-1 is composed of PCNA-like domain. However, RAD9 has an additional domain with a molecular weight of 13 kD in the C-terminus. Structure of 9-1-1 trimer of PCNA-like domains is similar to that of PCNA trimer. In this study, we prepared the C-terminal domain of RAD9CTD and performed structural analysis by small angle X-ray scattering(SAXS). Some archaea have heterotrimeric DNA clamp(PCNA1-PCNA2-PCNA3). We reveals that PCNA2-PCNA3 from Sulfolobus tokodaii forms tetrameric ring structure in solution by SAXS.
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Report
(4 results)
Research Products
(123 results)
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[Journal Article] Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex2013
Author(s)
N.Kuwabara, Y.Murayama, H.Hashimoto, Y.Kokabu, M.Ikeguchi, M.Sato, K.Mayanagi, Y.Tsutsui, H.Iwasaki,and T.Shimizu
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Journal Title
Structure
Volume: 20
Issue: 3
Pages: 440-449
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of a small molecule PCNA inhibitor that disrupts interactions with PIP-Box proteins and inhibits DNA replication2012
Author(s)
Chandanamali Punchihewa, Akira Inoue, Asami Hishiki, Yoshihiro Fujikawa, Michele Connelly, Benjamin Evison, Youming Shao, Richard Heath, Isao Kuraoka, Patrick Rodrigues, Hiroshi Hashimoto, Takashi Yagi, & Naoaki Fujii
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 14289-14300
Related Report
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[Journal Article] Identification of a small molecule PCNA inhibitor that disrupts interactions with PIP-Box proteins and inhibits DNA replication2012
Author(s)
Chandanamali Punchihewa, Akira Inoue, Asami Hishiki, Yoshihiro Fujikawa, Michele Connelly, Benjamin Evison, Youming Shao, Richard Heath, Isao Kuraoka, Patrick Rodrigues, Hiroshi Hashimoto, Masanobu Kawanishi, Mamoru Sato, Takashi Yagi, Naoaki Fujii
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Journal Title
J Biol Chem
Volume: 287
Issue: 17
Pages: 14289-14300
DOI
Related Report
Peer Reviewed
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[Journal Article] Crystal structure of the human centromeric nucleosome containing CENP-A2011
Author(s)
Tachiwana H, Kagawa W, Shiga T, Osakabe A, Miya Y, Saito K, Hayashi-Takanaka Y, Oda T, Sato M, Park SY, Kimura H, Kurumizaka H
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Journal Title
Nature
Volume: 476
Issue: 7359
Pages: 232-235
DOI
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Peer Reviewed
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[Journal Article] The Development of N-α-(2-Carboxyl)benzoyl-N5-(2-fluoro-1-iminoethyl)-L-The Development of N-α-(2-Carboxyl)benzoyl-N5-(2-fluoro-1-iminoethyl)-L-ornithine Amide (o-F-amidine) and N-α-(2-Carboxyl)benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine Amide (o-Cl-amidine) As Second Generation Protein Arginine Deiminase (PAD) Inhibitors2011
Author(s)
C.P.Causey, J.E.Jones, J.L.Slack, D.Kamei, L.E.Jones, Jr., V.Subramanian, B.Knuckley, P.Ebrahimi, A.A.Chumanevich, Y.Luo, H.Hashimoto, M.Sato, L.J.Hofseth P.R.Thompson
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Journal Title
J.Med.Chem.
Volume: 54
Issue: 19
Pages: 6919-6935
DOI
Related Report
Peer Reviewed
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[Journal Article] Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure2011
Author(s)
Kokabu Y, Murayama Y, Kuwabara N, Oroguchi T, Hashimoto H, Tsutsui Y, Nozaki N, Akashi S, Unzai S, Shimizu T, Iwasaki H, Sato M, Ikeguchi M
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Journal Title
J Biol Chem
Volume: 286
Issue: 50
Pages: 43569-43576
DOI
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Peer Reviewed
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[Journal Article] Structure and Functional Characterization of Vibrio parahaemolyticus Thermostable Direct Hemolysin2010
Author(s)
I.Yanagihara, K.Nakahira, T.Yamane, S.Kaieda, K.Mayanagi, D.Hamada, T.Fukui, K.Ohnishi, S.Kajiyama, T.Shimizu, M.Sato, T.Ikegami, M.Ikeguchi, T.Honda, H.Hashimoto
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Journal Title
J.Biol.Chem.
Volume: 285
Pages: 16267-16274
Related Report
Peer Reviewed
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[Journal Article] Deimination stabilizes histone H2A/H2B dimers as revealed by electrospray ionization mass spectrometry2010
Author(s)
Shimoyama, S., Nagadoi, A., Tachiwana, H., Yamada, M., Sato, M., Kurumizaka, H., Nishimura, Y., Akashi, S.
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Journal Title
J.Mass.Spectrom.
Volume: 45
Pages: 900-908
Related Report
Peer Reviewed
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[Journal Article] The structure of the N-terminal regulatory domain of a plant NADPH oxidase and its functional implications2009
Author(s)
Takashi Oda, Hiroshi Hashimoto, Naoyuki Kuwabara, Satoko Akashi, Kokoro Hayashi, Chojiro Kojima, Wong Hann Ling, Tsutomu Kawasaki, Ko Shimamoto, Mamoru Sato, Toshiyuki Shimizu
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Journal Title
J Biol Chem 285
Pages: 1435-45
Related Report
Peer Reviewed
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[Presentation] Crystal structure and biochemical property of the human centromere specific nucleosome2011
Author(s)
Hiroaki Tachibana, Wataru Kagawa, Tatsuya Shiga, Ryohei Matsumoto, Yuta Miya, Akihisa Osakabe, Yoko Hayashi-Takanaka, Takashi Oda, Mamoru Sato, Sam-Yong Park, Hiroshi Kimura, Hitoshi Kurumizaka
Organizer
第34回日本分子生物学会大会
Place of Presentation
パシフィコ横浜
Related Report
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[Presentation] Structure of REV7 in complex with a REV3 fragment and structural implication of the interaction between DNA polymerase F Nf and REV12011
Author(s)
Hiroshi Hashimoto, Kodai Hara, Yoshiki Murakumo, Shunsuke Kobayashi, Toshiaki Kogame, Satoru Unzai, Satoko Akashi, Shunichi Takeda, Toshiyuki Shimizu, Mamoru Sato
Organizer
Current Opinion in Structural Biology & DNA Repair, A Current Opinion in Structural Biology conference
Place of Presentation
NH Grand Hotel Krasnapolsky, Amsterdam, Netherlands
Related Report
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[Presentation] Crystal structure of thermostable direct hemolysin from Vibrio parahaemolyticus2010
Author(s)
Hiroshi Hashimoto, Kumiko Nakahira, Tsutomu Yamane, Takashi Fukui, Kiyohisa Ohnishi, Toshiyuki Shimizu, TakeshiHonda, Mamoru Sato, Mitsunori Ikeguchi, Itaru Yanagihara
Organizer
The 10th Conference of the Asian Crystallographic Association (AsCA2010)
Place of Presentation
BEXCO, Busan, Korea
Related Report
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[Presentation] ヒト由来Mad2L2の結晶学的研究2009
Author(s)
Kodai Hara, Toshiyuki Shimizu, Yoshiki Murakumo, Tomo Hanafusa, Haruo Ohmori, Mamoru Sato, Hiroshi Hashimoto
Organizer
American Crystallographic Association Meeting
Place of Presentation
Toronto at Canada
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