| Project/Area Number |
21370050
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | National Institutes of Natural Sciences Okazaki Research Facilities |
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Principal Investigator |
KATO Koichi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (20211849)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Takumi 分子科学研究所, 生命・錯体分子科学研究領域, 助教 (60522430)
KAMIYA Yukiko 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特任助教 (00527947)
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUSHIMA Tsunehiro 名古屋市立大学, 大学院・薬学研究科, 准教授 (90362269)
SATO Tadashi 名古屋市立大学, 大学院・薬学研究科, 准教授 (80532100)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 品質管理 / 糖鎖 / レクチン / NMR / 結晶構造解析 |
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| Research Abstract |
In cells, the carbohydrate moieties serve as tags for the quality control of glycoproteins through interactions with a variety of lectins. To elucidate the mechanisms underlying the glycoprotein quality control by the lectins that cycle between the endoplasmic reticulum and the Golgi, we provided the structural basis for their molecular recognition and complex formation by structural biological approaches such as X-ray crystallography. Furthermore, we successfully developed novel NMR methods for detailed analyses of conformational dynamics of the carbohydrate chains involved in this system.
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