Elucidation of structural basis for molecular recognition and supramolecular complex formation of intracellular lectins involved in post-endoplasmic reticulum quality control
Project/Area Number |
21370050
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | National Institutes of Natural Sciences Okazaki Research Facilities |
Principal Investigator |
KATO Koichi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (20211849)
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Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Takumi 分子科学研究所, 生命・錯体分子科学研究領域, 助教 (60522430)
KAMIYA Yukiko 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特任助教 (00527947)
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Co-Investigator(Renkei-kenkyūsha) |
MIZUSHIMA Tsunehiro 名古屋市立大学, 大学院・薬学研究科, 准教授 (90362269)
SATO Tadashi 名古屋市立大学, 大学院・薬学研究科, 准教授 (80532100)
|
Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | 品質管理 / 糖鎖 / レクチン / NMR / 結晶構造解析 |
Research Abstract |
In cells, the carbohydrate moieties serve as tags for the quality control of glycoproteins through interactions with a variety of lectins. To elucidate the mechanisms underlying the glycoprotein quality control by the lectins that cycle between the endoplasmic reticulum and the Golgi, we provided the structural basis for their molecular recognition and complex formation by structural biological approaches such as X-ray crystallography. Furthermore, we successfully developed novel NMR methods for detailed analyses of conformational dynamics of the carbohydrate chains involved in this system.
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Report
(4 results)
Research Products
(140 results)
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[Journal Article] A non-canonical UBA-UBL interaction forms the linear-ubiquitin-chain assembly complex2012
Author(s)
H. Yagi, K. Ishimoto, T. Hiromoto, H. Fujita, T. Mizushima, Y. Uekusa, M. Yagi-Utsumi, E. Kurimoto, M. Noda, S. Uchiyama, F. Tokunaga, K. Iwai and K. Kato
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Journal Title
EMBO reports
Volume: 13
Pages: 462-468
Related Report
Peer Reviewed
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[Journal Article] Structural basis for the cooperative interplay between the two causative gene products of combined factor V and factor VIII deficiency2010
Author(s)
M. Nishio, Y. Kamiya, T. Mizushima, S. Wakatsuki, H. Sasakawa, K. Yamamoto, S. Uchiyama, M. Noda, A. R. McKay, K. Fukui, H.-P. Hauri and K. Kato
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Journal Title
Proc. Natl. Acad. Sci. U. S. A.
Volume: 107
Pages: 4034-4039
Related Report
Peer Reviewed
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[Journal Article] Redox-dependent domain rearrangement of protein disulfide isomerase coupled with exposure of its substrate-binding hydrophobic surface2010
Author(s)
O. Serve, Y. Kamiya, A. Maeno, M. Nakano, C. Murakami, H. Sasakawa, Y. Yamaguchi, T. Harada, E. Kurimoto, M. Utsumi-Yagi, T. Iguchi, K. Inaba, J. Kikuchi, O. Asami, T. Kajino, T. Oka, M. Nakasako and K. Kato
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Journal Title
J. Mol. Biol.
Volume: 396
Pages: 361-374
Related Report
Peer Reviewed
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[Journal Article] Redox-dependent domain rearrangement of protein disulfide isomerase coupled with exposure of its substrate-binding hydrophobic surface2010
Author(s)
O.Serve, Y.Kamiya, A.Maeno, M.Nakano, C.Murakami, H.Sasakawa, Y.Yamaguchi, T.Harada, E.Kurimoto, M.Utsumi-Yagi, T.Iguchi, K.Inaba, J.Kikuchi, O.Asami, T.Kajino, T.Oka, M.Nakasako, K.Kato
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Journal Title
J.Mol.Biol. 396
Pages: 361-374
Related Report
Peer Reviewed
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[Journal Article] Structural basis for the cooperative interplay between the two causative gene products of combined factor V and factor VIII deficiency2010
Author(s)
M.Nishio, Y.Kamiya, T.Mizushima, S.Wakatsuki, H.Sasakawa, K.Yamamoto, S.Uchiyama, M.Noda, A.R.McKay, K.Fukui, H.-P.Hauri, K.Kato
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Journal Title
Proc.Natl.Acad.Sci.U.S.A. 107
Pages: 4034-4039
Related Report
Peer Reviewed
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[Journal Article] Silkworm expression and sugar profiling of human immune cell surface receptor, KIR2DL12009
Author(s)
K.Sasaki, M.Kajikawa, K.Kuroki, T.Motohashi, T.Shimojima, E.Y.Park, S.Kondo, H.Yagi, K.Kato, K.Maenaka
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Journal Title
Biochem.Biophys.Res.Commun. 387
Pages: 575-580
Related Report
Peer Reviewed
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[Presentation] A systematic approach to structural glycobiology2011
Author(s)
K. Kato, H. Yagi, Y. Kamiya, M. Nishio, T. Yamaguchi, S. Yamamoto, M. Yagi-Utsumi and T. Mizushima
Organizer
3rd Korea-Japan Seminar on Biomolecular Sciences-Experiments and Simulations
Place of Presentation
LOTTE Hotel(Jeju, Korea)
Year and Date
2011-02-27
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[Presentation] 複合糖質の構造・機能解析2010
Author(s)
加藤晃一
Organizer
岡崎統合バイオサイエンスセンター・サマースクール
Place of Presentation
岡崎統合バイオサイエンスセンター(愛知)
Year and Date
2010-08-18
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[Presentation] レクチンとEFバンドタンパク質の協働的相互作用による血液凝固因子の細胞内輸送の構造基盤2010
Author(s)
西尾美穂, 神谷由紀子, 水島恒裕, 若槻壮市, 笹川拡明, 山本一夫, 内山進, 野田勝紀, McKay Adam R, 福井希一, Hauri Hans-Peter, 加藤晃一
Organizer
第10回日本蛋白質科学会年会
Place of Presentation
札幌コンベンションセンター(北海道)
Year and Date
2010-06-17
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