pH regulation of organelles and its physiological role and molecular mechanism
| Project/Area Number |
21370055
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Chugoku Gakuen University (2011-2012)
Osaka University (2009-2010) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MITSUI Kaiji 大阪大学, 理学研究科, 助教 (60379279)
NAGAMORI Syushi 大阪大学, 医学研究科, 助教 (90467572)
MATSUSHITA Masafumi 大阪大学, 理学研究科, 助教 (50403100)
OHGAKI Ryuichi 大阪大学, 医学研究科, 助教 (20467525)
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| Project Period (FY) |
2009 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | エンドサイトーシス / 酵母の NHE / 細胞内局所p H 制御 / 細胞内蛋白質局在化 機構 / 細胞内 pH 調節 Na+/H+ 交換輸送体 / 細胞内小胞型 NHE / 細胞内蛋白質の輸送 / 細胞内pH調節 / Na+/H+交換輸送体 / 細胞内小胞型NHE / 酵母のNHE / 細胞内局所ｐH制御 / 細胞内蛋白質局在化機構 / Na^+/H^+交換輸送体 / 生体膜イオン輸送 / pH制御 / 膜小胞輸送 / NHE / 細胞内イオン環境 / Na+/H+交換輸送蛋白質 / オルガネラ局在 / pH調節 / 細胞内小胞輸送機構 / 蛋白質の局在化機構 / 細胞内小胞pH測定 / 細胞極性形成 / 膜イオン輸送 |
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| Research Abstract |
Na+/H+ exchangers (NHE. Or Nha) are found in the plasma or endosome membranes for the most cells of living organisms. Cytoplasmic pH is maintained at 7 while intracellular ensodome pH is set at weak acidic pH. NHEs are provided to regulate the cytoplasmic and endosome pH. However, their physiological function is still not clarified. In the present study, we focused on the physiological role of NHE localized at various endosomes and found a novel role in the intracellular protein trafficking.
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Report
(4 results)
Research Products
(77 results)
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[Journal Article] Na+/H+ exchanger isoform 6 (NHE6/ SLC9A6) is involved in clathrin-dependent endocytosis of transferrin2011
Author(s)
Lou, X., Matsushita, M., Numaza, M., Taguchi, Mitsui, K., and Kanazawa, H.
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Journal Title
Am. J. Physiol. Cell Physiol.
Volume: 301
Related Report
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[Journal Article] James1Quantitative proteomic analysis of the adipocyte plasma membrane2011
Author(s)
Matthew J. Prior1, Mark Larance1, Robert T. Lawrence1, Jamie Soul1, SeanHumphrey1, James Burchfield1, Carol Kistler2, Jonathon R. Davey1, Penelope J. La-Borde1, Michael Buckley3, Hiroshi Kanazawa4, Robert G. Parton4, MichaelGuilhaus5 and David E.
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Journal Title
J. Proteome. Res
Volume: 10
Pages: 4970-4982
Related Report
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[Journal Article] Evidence that 'Brain-Specific' Fox-1, Fox-2, and nPTB alternatively spliced isoformsare produced in the lens.2011
Author(s)
122. Bitel, C.L., Nathan, R., Wong, P., Kuppasani, S., Matsushita, M., Kanazawa, H., and Frederikse, P.H.
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Journal Title
Curr. Eye Res.
Volume: 36
Pages: 321-327
Related Report
Peer Reviewed
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[Journal Article] A loss-of-function mutation in the SLC9A6 gene causes X-linked mental retardation resembling Angelman syndrome.2011
Author(s)
Takahashi, Y., Hosoki, K., Matsushita, M., Funatsuka, M., Saito, K.,, Kanazawa, H., Goto, Y., and Saitoh, S.
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Journal Title
Am. J. Med. Genet. B: Neuropsychiatr. Genet.
Volume: 156
Pages: 799-807
Related Report
Peer Reviewed
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[Journal Article] Quantitative proteomic analysis of the adipocite plasma membrane.2011
Author(s)
Prior, M. J., Larance, M., Lawrence, R. T., Kistler, C., Davey, J. R., La-Borde, P. J., Buckley, M., Kanazawa, H., Parton, R. G., Guilhaus, M., and James, D. E.
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Journal Title
J. Proteome Res.
Volume: 10
Pages: 4970-4982
Related Report
Peer Reviewed
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[Journal Article] Na+/H+ exchanger isoform 6 (NHE6 / SLC9A6) is involved in clathrin-dependent endocytosis of transferrin2011
Author(s)
Lou, X., Matsushita, M., Numaza, M., Taguchi, A., Mitsui, K., and Kanazawa, H.
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Journal Title
Am. J. Physiol. Cell Physiol.
Volume: 301
Pages: 1431-1444
Related Report
Peer Reviewed
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