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Molecular mechanism of the multi-transportmachinery

Research Project

Project/Area Number 21370057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionOkayama University

Principal Investigator

OMOTE Hiroshi  岡山大学, 大学院・医歯薬学総合研究科, 准教授 (10273707)

Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords生体膜 / トランスポーター / タンパク質 / 生化学 / 神経 / 輸送 / グルタミン酸 / SLC17 / VGLUT / 多機能性 / 小胞型グルタミン酸トランスポーター / 小胞型ヌクレオチドトランスポーター / VNUT / 二価金属イオン / ケトン体 / 塩素イオン / 制御 / VEAT / NPT / リン酸輸送
Research Abstract

The vesicular glutamate transporter (VGLUT) is a member of the SLC17 family and transports glutamate into synaptic vesicles using a membrane potential as a driving force. VGLUT plays essential roles in glutamatergic signal transmission. The SLC17 family members are unique transporter which two distinct transport activity ,membrane potential driven anion transport and Na^+ gradient driven inorganic phosphate transport. In this study, we investigated functional mechanism and roles of SLC17 transporters.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (38 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (15 results) (of which Peer Reviewed: 10 results) Presentation (17 results) Remarks (1 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 3 results)

  • [Journal Article] Vesicular neurotransmitter transporters: an approach for studying transporters with purified proteins.2013

    • Author(s)
      Omote H
    • Journal Title

      Physiology

      Volume: 28 Issue: 1 Pages: 39-50

    • DOI

      10.1152/physiol.00033.2012

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Na+/phosphate co-transporter homologue (SLC17A4 protein) is an intestinal organic anion exporter.2012

    • Author(s)
      Togawa N
    • Journal Title

      Am J Physiol Cell Physiol

      Volume: 302 Issue: 11 Pages: C1652-C1660

    • DOI

      10.1152/ajpcell.00015.2012

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Divalent cation transport by vesicular nucleotide transporter2011

    • Author(s)
      Miyaji, T., Sawada, K., Omote, H.Moriyama Y
    • Journal Title

      J. Biol. Chem

      Volume: 286 Issue: 50 Pages: 42881-42887

    • DOI

      10.1074/jbc.m111.277269

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of vesicular excitatory amino acid transport by human sialin.2011

    • Author(s)
      Miyaji, T., Omote, H.Moriyama Y
    • Journal Title

      J.Neurochem

      Volume: 119 Pages: 1-5

    • Related Report
      2012 Final Research Report
  • [Journal Article] Vesicular neurotransmitter transporter: bioenergetics and regulation of glutamate transport2011

    • Author(s)
      Omote, H.Miyaji, T., Juge, N. and Moriyama, Y
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 25 Pages: 5558-5565

    • DOI

      10.1021/bi200567k

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of he human MATE2 proton-coupled polyspecific organic cation exporter2011

    • Author(s)
      Komatsu, T., Hiasa, M., Miyaji, T.,Kanamoto, T., Matsumoto, T., Otsuka, M., Moriyama, Y. and Omote, H
    • Journal Title

      In.t J. Biochtem. Cell Biol.

      Volume: 43 Issue: 6 Pages: 913-918

    • DOI

      10.1016/j.biocel.2011.03.005

    • Related Report
      2012 Final Research Report
  • [Journal Article] Functional characterization of vesicular excitatory amino acid transport by human sialin2011

    • Author(s)
      Miyaji T, Omote H, Moriyama Y
    • Journal Title

      J Neurochem

      Volume: 119 Issue: 1 Pages: 1-5

    • DOI

      10.1111/j.1471-4159.2011.07388.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic control of vesicular glutamate transport and release2010

    • Author(s)
      Juge, N., Gray, J.A.,Omote, H.Miyaji, N doi: ne Iharada, M., Miyaji, T., Fujimoto, T., Hiasa, T., Inoue, T., Hara, C., Uneyama, H., Edwards, R.H., Nicoll, R.A. and Moriyama, Y.
    • Journal Title

      Neuron

      Volume: 68 Pages: 99-112

    • Related Report
      2012 Final Research Report
  • [Journal Article] Type 1 sodium dependent phosphate transporter (SLC17A1 protein) is a Cl^- -dependent urate exporter2010

    • Author(s)
      Iharada, M., Miyaji, T., Fujimoto, T., Hiasa,M., Anzai, N., Omote, H., and Moriyama, Y
    • Journal Title

      J. Biol. Chem

      Volume: 285 Issue: 34 Pages: 26107-26113

    • DOI

      10.1074/jbc.m110.122721

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins2010

    • Author(s)
      Sonoda, Y., Cameron, A., Newstead,S., Omote, H., Moriyama, Y., Kasahara, M., Iwata, S., Drew, D.
    • Journal Title

      FEBS

      Volume: 584 Pages: 2539-2547

    • Related Report
      2012 Final Research Report
  • [Journal Article] A vesicular transporter that mediates aspartate and glutamate neurotransmission.2010

    • Author(s)
      Miyaji T, et al
    • Journal Title

      Biol Pharm Bull

      Volume: 33 Pages: 1783-1785

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic control of vesicular glutamate transport and release.2010

    • Author(s)
      Juge N, et al
    • Journal Title

      Neuron

      Volume: 68 Pages: 99-112

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Type 1 sodium-dependent phosphate transporter (SLC17A1 Protein) is a Cl^--dependent urate exporter2010

    • Author(s)
      Iharada M, et al
    • Journal Title

      J Biol Chem.

      Volume: 285 Pages: 26107-26113

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vesicular inhibitory amino acid transporter is a Cl^-/γ-aminobutyrate co-transporter.2009

    • Author(s)
      Juge, N.,Muroyama, A., Hiasa, M., Omote, H.and Moriyama, Y.
    • Journal Title

      J. Biol. Chem

      Volume: 284 Pages: 35073-35078

    • Related Report
      2012 Final Research Report
  • [Journal Article] Vesicular inhibitory amino acid transporter is a Cl-/gamma-aminobutyrate Co-transporter.2009

    • Author(s)
      樹下成信、室山明子、日浅未来、表弘志、森山芳則
    • Journal Title

      The Journal of Biological Chemistry 284

      Pages: 35073-35078

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Metabolic Control of Vesicular Glutamate Transporter2012

    • Author(s)
      表弘志
    • Organizer
      日本生理学会
    • Place of Presentation
      信州大学(松本)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] NPTホモログは新規尿酸排出トランスポーターである2012

    • Author(s)
      表 弘志
    • Organizer
      日本生体エネルギー研究会 第38回討論会
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] NPTホモログは新規尿酸排出トランスポーターである2012

    • Author(s)
      表 弘志
    • Organizer
      生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] NPTホモログは新規尿酸排出トランスポーターである2012

    • Author(s)
      表弘志
    • Organizer
      日本生体エネルギー研究会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 小胞型グルタミン酸トランスポーターの活性制御2011

    • Author(s)
      表弘志
    • Organizer
      生体エネルギー研究会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2011-12-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 小胞型グルタミン酸トランスポーターの機能制御2011

    • Author(s)
      表弘志
    • Organizer
      生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      岡山大学創立50周年記念館(岡山)
    • Year and Date
      2011-11-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 小胞型グルタミン酸トランスポーターの機能制御2011

    • Author(s)
      表弘志
    • Organizer
      日本生化学会
    • Place of Presentation
      国際会議場(京都)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 小胞型グルタミン酸トランスポーターのCl-イオンによる制御2011

    • Author(s)
      表弘志
    • Organizer
      日本生化学会中国四国支部例会
    • Place of Presentation
      応仁会館(広島)
    • Year and Date
      2011-05-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 小胞型グルタミン酸トランスポーターの活性制御2011

    • Author(s)
      表 弘志
    • Organizer
      日本生体エネルギー研究会 第36回討論会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 小胞型神経伝達物質トランスポーターの機能制御2011

    • Author(s)
      表 弘志
    • Organizer
      生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] 小胞型ヌクレオチドトランスポーター(VNUT)は2価金属イオンを輸送する2010

    • Author(s)
      表弘志
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 小胞型ヌクレオチドトランスポーターによる2価カチオン輸送2010

    • Author(s)
      表弘志
    • Organizer
      生体エネルギー研究会
    • Place of Presentation
      大阪
    • Year and Date
      2010-11-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] 小胞型ヌクレオチドトランスポーターによる二価カチオン輸送2010

    • Author(s)
      表 弘志
    • Organizer
      日本生体エネルギー研究会 第36回討論会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] 小胞型抑制性アミノ酸トランスポーター(VIAAT)によるCl-輸送2009

    • Author(s)
      表弘志
    • Organizer
      生体エネルギー研究会
    • Place of Presentation
      旭川医科大学(北海道)
    • Year and Date
      2009-12-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 小胞型神経伝達物質トランスポーターとシアル酸輸送2009

    • Author(s)
      表弘志
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 小胞型神経伝達物質トランスポーターとシアル酸輸送2009

    • Author(s)
      表 弘志
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] 小胞型抑制性アミノ酸トランスポーター(VIAAT)による2009

    • Author(s)
      表 弘志
    • Organizer
      Cl-輸送日本生体エネルギー研究会 第35回討論会
    • Place of Presentation
      旭川医科大学
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/seika/home/index.html

    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 哺乳動物における新規SLC17型トランスポータータンパク質およびその利用2013

    • Inventor(s)
      森山 芳則、表 弘志、澤田 啓介
    • Industrial Property Rights Holder
      岡山大学
    • Acquisition Date
      2013-03-15
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] Novel SLC-17-type transporter protein in mammal and use thereof2012

    • Inventor(s)
      Moriyama, Y., Omote, H., Sawada, K
    • Industrial Property Rights Holder
      岡山大学
    • Acquisition Date
      2012-09-26
    • Related Report
      2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 哺乳動物における新規トランスポータータンパク質およびその利用2011

    • Inventor(s)
      森山 芳則、表 弘志、大塚 正人、松本 拓也、日浅 未来
    • Industrial Property Rights Holder
      岡山大学
    • Acquisition Date
      2011-06-03
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] Methods for screening modulators of SLC17-type anion transport activity2011

    • Inventor(s)
      Moriyama, Y., Omote, H., Sawada, K
    • Industrial Property Rights Holder
      岡山大学
    • Acquisition Date
      2011-05-31
    • Related Report
      2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] Novel Transporter protein in mammal and utilization of the same2010

    • Inventor(s)
      Moriyama, Y., Omot ., Otsuka, M.,Matsumoto, T., Hiasa, M
    • Industrial Property Rights Holder
      岡山大学
    • Acquisition Date
      2010-11-04
    • Related Report
      2012 Final Research Report
    • Overseas

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Published: 2009-04-01   Modified: 2019-07-29  

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