Structural polymorphism and functional differentiation of actin filaments
Project/Area Number |
21370061
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
UYEDA Taro 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 部門付 (90356551)
|
Co-Investigator(Kenkyū-buntansha) |
TOKURAKU Kiyotaka 室蘭工業大学, 大学院・工学研究科, 准教授 (00332106)
KATAYAMA Eisaku 千葉大学, 大学院・工学研究科, 研究員 (50111505)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2010: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
|
Keywords | アクチンフィラメント / ミオシン / コフィリン / 協同的結合 / 張力 / メカノセンサー / 細胞極性 / アクチン / アクチン結合タンパク質 / 協同的構造変化 / GFP |
Research Abstract |
The goal of this project is to understood how different actin filaments within a cell perform different functions. We found that stretched actin filaments with a longer helical pitch have higher affinity for myosin II. We also found that myosin II and cofilin, two major actin binding proteins, bound to actin filaments in a mutually exclusive manner, even though the two proteins do not compete for a binding site on actin. These results support our hypothesis that actin filaments of different atomic structures have different affinities for different actin binding proteins, leading to functional differentiation within cells.
|
Report
(4 results)
Research Products
(52 results)
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[Journal Article] G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility2012
Author(s)
Noguchi, T. Q. P., T. Komori, N. Umeki N. Demizu, K. Ito, A. Hikikoshi-Iwane, K. Tokuraku, T. Yanagida and T. Q. P.Noguchi, T. Q. P., T. Komori, N. Umeki N. Demizu, K. Ito, A. Hikikoshi-Iwane, K. Tokuraku, T. Yanagida and T. Q. P. Uyeda
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Journal Title
J. Biol. Chem.
Volume: (in press)
URL
Related Report
Peer Reviewed
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[Journal Article] Actin interaction and regulation of cyclin-dependent kinase 5/P35 complex activity.2011
Author(s)
Xu, J., Tsutsumi, K., Tokuraku, K., Estes, K.A., Hisanaga, S., Ikezu, T.
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Journal Title
Journal of Neurochemistry
Volume: 116
Pages: 192-204
Related Report
Peer Reviewed
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[Journal Article] Electron microscopic evidence for the myosin head lever arm mechanism in hydrated myosin filaments using the gas environmental chamber.2011
Author(s)
Minoda, H., Okabe, T., Inayoshi, Y., Miyakawa, T., Miyauchi, Y., Tanokura, M., Katayama, E., Wakabayashi, T., Akimoto, T., Sugi, H.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 405
Pages: 651-656
Related Report
Peer Reviewed
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