Exploring novel human endogenous lectins and their functional analysis

• Project/Area Number: 21370062
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: HIRABAYASHI Jun 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, 副研究センター長 (40156691)
• Co-Investigator(Kenkyū-buntansha): TATENO Hiroaki 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, 研究員 (30450670)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: レクチン / 糖鎖 / 真菌 / 免疫

Research Abstract

In order to elucidate biological functions of glycans, it is essential to make a comprehensive approach to their partner molecules, endogeneous lectins. A considerably large number of lectin candidate molecuoles are found in the human geneome data base. Based on the information it is now possible to produce their recombinant proteins and investigate systematically their sugar-binding properties by means of advanced glycotechnologies developed in our group, e. g., glycoconjugate microarray and frontal affinity chromatography. Both of their possible roles in vivo and utilities as tools are studied.

Research Products

• [Journal Article] Directed evolution of lectins with a novel sugar-binding specificity for 6-sulfo-galactose (2012)
  • Author(s): Dan Hu, Hiroaki Tateno, Atsushi Kuno, Rikio Yabe, Jun Hirabayashi
  • Journal Title: J.Biol.Chem. Volume: (in press) Issue: 24 Pages: 20313-20320
  • DOI: 10.1074/jbc.m112.351965
  • Peer Reviewed
• [Journal Article] Human ZG16p recognizes pathogenic fungi through non-self polyvalent mannose in the digestive system (2012)
  • Author(s): Tateno H, Yabe R, Sato T, Shibazaki A, Shikanai T, Gonoi T, Narimatsu H, Hirabayashi J
  • Journal Title: Glycobiology Volume: 22(2) Issue: 2 Pages: 210-20
  • DOI: 10.1093/glycob/cwr130
  • Peer Reviewed
• [Journal Article] Mannose-specific lectin from the mushroom Hygrophorus russula (2012)
  • Author(s): Suzuki, T.
  • Journal Title: Glycobiology Volume: 22 Issue: 5 Pages: 616-629
  • DOI: 10.1093/glycob/cwr187
  • Peer Reviewed
• [Journal Article] Purification, Characterization, and Molecular Cloning of Lectin from Winter Buds of Lysichiton camtschatcensis (L.) Schott (2012)
  • Author(s): Nakagawa, Y.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 1 Pages: 25-33
  • DOI: 10.1271/bbb.110346
  • NAID: 10030398242
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Difference in fine specificity to polysaccharides of C.albicans mannoprotein between mouse SIGNR1 and human DC-SIGN (2012)
  • Author(s): Takahara, K.
  • Journal Title: Infect.Immun. Volume: 80 Issue: 5 Pages: 1699-1706
  • DOI: 10.1128/iai.06308-11
  • Peer Reviewed
• [Journal Article] The Galβ-(syn)-gauche configuration is required for galectin-recognition disaccharides (2011)
  • Author(s): Iwaki J, Tateno H, Nishi N, Minamisawa T, Nakamura-Tsuruta S, Itakura Y, Kominami J, Urashima T, Nakamura T, Hirabayashi J
  • Journal Title: Biochim Biophys Acta Volume: 1810(7) Issue: 7 Pages: 643-51
  • DOI: 10.1016/j.bbagen.2011.04.001
  • Peer Reviewed
• [Journal Article] Profiling the Cell Surface Glycome of Five Fungi Using Lectin Microarray (2011)
  • Author(s): Shibazaki, A.
  • Journal Title: J.Carbohydr.Chem. Volume: 30 Issue: 3 Pages: 147-164
  • DOI: 10.1080/07328303.2011.619674
  • Peer Reviewed
• [Journal Article] Structure and binding analysis of Polyporus squamosus lectin in complex with the Neu5Ac{alpha}2-6Gal{beta}1-4GlcNAc human-type influenza (2011)
  • Author(s): Kadirvelraj, R.
  • Journal Title: Glycobiology Volume: 21 Issue: 7 Pages: 973-984
  • DOI: 10.1093/glycob/cwr030
  • Peer Reviewed
• [Journal Article] Engineering of the Glycan-binding Specificity of Agrocybe cylindracea Galectin toward {alpha} (2,3)-linked Sialic Acid by Saturation (2011)
  • Author(s): Imamura, K.
  • Journal Title: J.Biochem. Volume: 150 Issue: 5 Pages: 545-552
  • DOI: 10.1093/jb/mvr094
  • Peer Reviewed
• [Journal Article] Role of Malectin in Glc2Man9GlcNAc2-dependent quality control of {alpha}1-antitrypsin (2011)
  • Author(s): Chen, Y.
  • Journal Title: Mol.Biol.Cell. Volume: 22 Issue: 19 Pages: 3559-3570
  • DOI: 10.1091/mbc.e11-03-0201
  • Peer Reviewed
• [Journal Article] High-resolution structural insights on the sugar-recognition and fusion tag properties of a versatile {beta}-trefoil lectin domain from the mushroom Laetiporus sulphureus (2011)
  • Author(s): Angulo, I.
  • Journal Title: Glycobiology Volume: 21 Issue: 10 Pages: 1349-1361
  • DOI: 10.1093/glycob/cwr074
  • Peer Reviewed
• [Journal Article] Dual specificity of Langerin to sulfated and mannosylated glycans via a single C-type carbohydrate recognition domain (2010)
  • Author(s): Tateno H, Ohnishi K, Yabe R, Hayatsu N, Sato T, Takeya M, Narimatsu H, Hirabayashi J
  • Journal Title: J Biol Chem Volume: 285(9) Issue: 9 Pages: 6390-400
  • DOI: 10.1074/jbc.m109.041863
  • Peer Reviewed
• [Journal Article] Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans (2010)
  • Author(s): Yabe R, Tateno H, Hirabayashi J
  • Journal Title: FEBS J Volume: 277(19) Issue: 19 Pages: 4010-26
  • DOI: 10.1111/j.1742-4658.2010.07792.x
  • Peer Reviewed
• [Journal Article] SUEL-related lectins, a lectin family widely distributed throughout or ganisms. (2010)
  • Author(s): Hiroaki Tateno
  • Journal Title: Biosci Biotechnol Biochem. Volume: 74 Pages: 1141-1144
  • Peer Reviewed
• [Journal Article] Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans. (2010)
  • Author(s): Rikio Yabe
  • Journal Title: FEBS J. Volume: 277 Pages: 4010-4026
  • Peer Reviewed
• [Journal Article] Laetiporus sulphureus lectin and aerolysin protein family. (2010)
  • Author(s): Mancheno JM
  • Journal Title: FEBS J. Volume: 277 Pages: 4010-4026
  • Peer Reviewed
• [Journal Article] Dual specificity of Langerin to sulfated and mannosylated glycans via a single C-type carbohydrate recognition domain. (2010)
  • Author(s): Hiroaki Tateno
  • Journal Title: J Biol Chem. Volume: 285 Pages: 6390-6400
  • Peer Reviewed
• [Journal Article] The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation. (2010)
  • Author(s): Kaoru Mikami
  • Journal Title: J Biol Chem. Volume: 285 Pages: 310-321
  • Peer Reviewed
• [Journal Article] N-terminal specific point-immobilization of active proteins by the one-pot NEXT-A method (2009)
  • Author(s): Keitaro Ebisu
  • Journal Title: Chembiochem 10 Pages: 2460-2464
  • Peer Reviewed
• [Journal Article] C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. (2009)
  • Author(s): Sho Yamasaki
  • Journal Title: Proc.Natl.Acad.Sci. 106 Pages: 1897-1902
  • Peer Reviewed
• [Journal Article] Strict binding specificity of small-sized lectins from the red alga Hypnea japonica for core(alpha1-6)fucosylated N-glycans. (2009)
  • Author(s): Satomi Okuyama
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 912-920
  • Peer Reviewed
• [Journal Article] Human C21orf63 is a heparin-binding protein. (2009)
  • Author(s): Kanae Mitsunaga
  • Journal Title: J.Biochem. 146 Pages: 369-373
  • Peer Reviewed
• [Presentation] レクチン工学のすすめ (2011)
  • Author(s): 平林淳
  • Organizer: GlycoTOKYO 2011シンポジウム
  • Place of Presentation: 和光(招待)
  • Year and Date: 2011-12-09
• [Presentation] レクチンマイクロアレイを活用した幹細胞評価技術の開発 (2011)
  • Author(s): 舘野浩章
  • Organizer: 第3回JITA技術交流会
  • Place of Presentation: 第3回JITA技術交流会
  • Year and Date: 2011-01-07
• [Presentation] レクチンアレイ基盤細胞グライコミクスによる細胞評価システムの開発 (2010)
  • Author(s): 舘野浩章
  • Organizer: 第8回糖鎖科学コンソーシアム
  • Year and Date: 2010-11-30
• [Presentation] Lectin-based structural glycomics : development of lectin microarray (2010)
  • Author(s): Hirabayashi J
  • Organizer: 17th International Symposium and Exhibit on Liquid Phase Separation and Capillary Electroseparation Techniques
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2010-08-31
• [Presentation] Further advancement in lectin microarray : how many lectins are necessary for cell typing? (2010)
  • Author(s): Jun Hirabayashi
  • Organizer: Sialoglyco2010
  • Place of Presentation: Potsudam (Germany)
  • Year and Date: 2010-08-24
• [Presentation] Human ZG16 is a novel member of mannose-specific Jacalin-related lectins, which recognizes pathogenic fungi in the intestinal tract (2010)
  • Author(s): Hiroaki Tateno
  • Organizer: GlycoT2010
  • Place of Presentation: KFC (Kokusai Fashion Center) Hall Annex
  • Year and Date: 2010-08-01
• [Presentation] Human ZG16 is a novel member of mannose-specific Jacalin-related lectins, which recognizes pathogenic fungi in the intestinal tract (2010)
  • Author(s): Hiroaki Tateno
  • Organizer: The 28th Naito Conference Program
  • Place of Presentation: 国際湘南村
  • Year and Date: 2010-07-30
• [Presentation] Further advancement in lectin microarray : how many lectins are necessary for cell typing? (2010)
  • Author(s): Hirabayashi J
  • Organizer: Sialoglyco 2010
  • Place of Presentation: Potsdam, Germany
• [Presentation] Human ZG16 recognizes pathogenic fungi through specific recognition of non-self polyvalent mannose in the intestinal tract (2010)
  • Author(s): Tateno H
  • Organizer: GlycoT2010
  • Place of Presentation: Tokyo
• [Presentation] Medical application of lectin microarray : a powerful technology for differential glycan profiling (2010)
  • Author(s): Hirabayashi J
  • Organizer: The 28th NAITO Conference on Glycan Expression and Regulation[I]
  • Place of Presentation: Shonan Village Center, Japan
• [Presentation] Human ZG16 is a novel member of mannose-specific Jacalin-related lectins, which recognizes pathogenic fungi in the intestinal tract (2010)
  • Author(s): Tateno H
  • Organizer: 第28回内藤コンファレンス
  • Place of Presentation: 湘南国際村
• [Presentation] Cellular glycomics : a bridge over functional genomics and system biology (2009)
  • Author(s): Hirabayashi J
  • Organizer: 日本分子生物学会(MBSJ2009)
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] 細胞グライコミクスの推進と内在性レクチン機能解析 (2009)
  • Author(s): 舘野浩章
  • Organizer: 第7回糖鎖科学コンソーシアム
  • Place of Presentation: 大阪府・豊中市
  • Year and Date: 2009-12-08
• [Presentation] Langerinは硫酸化糖鎖とマンノース含有糖鎖への特異性を介して腫瘍細胞と病原真菌に結合する2重特異性を獲得した分子である (2009)
  • Author(s): 舘野浩章
  • Organizer: 第82回生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] A strategy for creation of artificial lectins based on the possible scenario on the origin and evolution of saccharides (2009)
  • Author(s): Hirabayashi J
  • Organizer: 第82日本生化学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] Langerin recognizes malignant and pathogenic cells through specificity to sulfated and mannosylated glycans via a single C-type carbohydrate recognition domain(英語) (2009)
  • Author(s): 舘野浩章
  • Organizer: 日墺二国間セミナー
  • Place of Presentation: 湘南国際村
  • Year and Date: 2009-10-14
• [Presentation] 細胞グライコームの推進と内在性レクチン機能解析 (2009)
  • Author(s): 舘野浩章
  • Organizer: 第7回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
• [Presentation] Langerin recognizes malignant and pathogenic cells through specificity to sulfated and mannosylated glycans via a single C-type carbohydrate recognition domain (2009)
  • Author(s): Tateno H, Yabe R, Sato T, Narimatsu H, Hirabayashi J
  • Organizer: 2009 Annual Meeting of the Society for Glycobiology
  • Place of Presentation: San Diego
• [Remarks] LfDB :レクチンフロンティアデータベースこれまで当該研究グループが独自、ないし共同研究によって解析した数百種に上るレクチンの特異性解析結果(FACによる)をまとめた公開サイト
  • URL: http://riodb.ibase.aist.go.jp/rcmg/glycodb/LectinSearch