| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Research Abstract |
Rapture force of single ribosome and mRNA was measured by optical tweezers. We found that IF2 helped to lock the 70S ribosome over the start codon during initiation, thus maintaining reading frame. Next, we studied chaperonin reaction cycle. It is widely believed that an asymmetric GroEL-GroES complex(termed a bullet-shaped complex) is the only form of the GroEL-GroES complex, and a symmetric GroEL-(GroES)_2 complex(termed a football-shaped complex) is not formed during the reaction cycle. However, we found that football-shaped complex was formed in the presence of sufficient amount of denatured proteins.
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