| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Research Abstract |
To elucidate detailed conformational change and protein folding mechanism of the group 2 chaperonin(CPN), we have performed kinetic studies of conformational change by stopped-flow fluorometry, stopped-flow small angle X-ray scattering and Diffracted X-ray Tracking. The results have shown that the conformational change is biphasic and the latter includes the rotational motion of the ring. In addition, we constructed and characterize asymmetric ring complex that consists of a wild-type ring and a mutant ring by using circular permutated connected mutants. The results gave an insight on the inter ring communication of CPN.
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