Elucidation of mechanism for conformational change and protein folding of the group 2 chaperonin

• Project/Area Number: 21370067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: YOHDA Masafumi 東京農工大学, 大学院・工学研究院, 教授 (50250105)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: シャペロン / シャペロニン / フォールディング / 構造変化 / 古細菌 / ストップトフロー / ストップドフロー

Research Abstract

To elucidate detailed conformational change and protein folding mechanism of the group 2 chaperonin(CPN), we have performed kinetic studies of conformational change by stopped-flow fluorometry, stopped-flow small angle X-ray scattering and Diffracted X-ray Tracking. The results have shown that the conformational change is biphasic and the latter includes the rotational motion of the ring. In addition, we constructed and characterize asymmetric ring complex that consists of a wild-type ring and a mutant ring by using circular permutated connected mutants. The results gave an insight on the inter ring communication of CPN.

Research Products

• [Journal Article] The N-terminal replacement of an olfactory receptor for the development of a yeast-based biomimetic odor sensor (2012)
  • Author(s): Fukutani Y, Nakamura T, Yorozu M, Ishii J, Kondo A, Yohda M.
  • Journal Title: Biotechnol Bioeng Volume: 109 Issue: 1 Pages: 205-212
  • DOI: 10.1002/bit.23327
  • Peer Reviewed
• [Journal Article] Amyloid oligomer detection by immobilized molecular chaperone (2012)
  • Author(s): Sakono M. Zako T, Yohda M, Maeda M.
  • Journal Title: Biochemical Engineering Journal Volume: 61 Pages: 28-33
  • DOI: 10.1016/j.bej.2011.12.005
  • Peer Reviewed
• [Journal Article] Dimer structure and conformational variability in the N-terminal region of an archaeal small heat shock protein (2011)
  • Author(s): Takeda, K., Hayashi, T., Abe, T., Hirano, Y., Hanazono, Y., Yohda, M., Miki, K.
  • Journal Title: J. Struct. Biol Volume: 174 Pages: 92-99
  • Peer Reviewed
• [Journal Article] StHsp14. 0, a small heat shock protein of Sulfolobus tokodaii strain 7, protects denatured proteins from aggregation in the partially dissociated conformation (2011)
  • Author(s): Abe, T., Oka, T., Nakagome, A., Tsukada, Y., Yasunaga, T., Yohda, M.
  • Journal Title: J. Biochem Volume: 150 Pages: 403-409
  • Peer Reviewed
• [Journal Article] Dimer structure and conformational variability in the N-terminal region of an archaeal small heat shock protein, StHsp14.0 (2011)
  • Author(s): Takeda K, Hayashi T, Abe T, Hirano Y Hanazono Y, Yohda M, Miki K.
  • Journal Title: J Struct Biol Volume: 174 Issue: 1 Pages: 92-99
  • DOI: 10.1016/j.jsb.2010.12.006
  • Peer Reviewed
• [Journal Article] Monooxygenation by a thermophilic cytochrome P450 via direct electron donation from NADH (2011)
  • Author(s): H. Matsumura
  • Journal Title: Metallomics Volume: 3 Issue: 4 Pages: 389-395
  • DOI: 10.1039/c0mt00079e
  • Peer Reviewed
• [Journal Article] StHsp14.0, a small heat shock protein of Sulfolobus tokodaii strain 7, protects denatured proteins from aggregation in the partially dissociated conformation (2011)
  • Author(s): Abe T, Oka T, Nakagome A, Tsukada Y Yasunaga T, Yohda M.
  • Journal Title: J Biochem Volume: 150 Issue: 4 Pages: 403-409
  • DOI: 10.1093/jb/mvr074
  • NAID: 10030588087
  • Peer Reviewed
• [Journal Article] Properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8 (2011)
  • Author(s): Igari S, Ohtaki A, Yamanaka Y, Sato Y, Yohda M, Odaka M, Noguchi K, Yamada K.
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Solubilization and folding of a fully active recombinant Gaussia luciferase with native disulfide bonds by using a SEP-tag (2011)
  • Author(s): Rathnayaka T, Tawa M, Nakamura T, SohyaS, Kuwajima K, Yohda M, Kuroda Y
  • Journal Title: Biochim Biophys Acta. Proteins and Proteomics Volume: 1814(12) Issue: 12 Pages: 1775-1778
  • DOI: 10.1016/j.bbapap.2011.09.001
  • Peer Reviewed
• [Journal Article] Size-selective recognition of gold nanoparticles by a molecular chaperone. (2011)
  • Author(s): Sakono M, Zako T, Drakulic S, Valpuesta JM, Yohda M, Maeda M.
  • Journal Title: Chem Phys Letters Volume: 501 Pages: 108-112
  • Peer Reviewed
• [Journal Article] Dimer structure and conformational variability in the N-terminal region of anarchaeal small heat shock protein, StHsp14.0. (2011)
  • Author(s): Takeda K, Hayashi T, Abe T, Hirano Y , Hanazono Y, Yohda M, Miki K.
  • Journal Title: J Struct Biol. Volume: 174 Pages: 92-99
  • Peer Reviewed
• [Journal Article] Thermodynamic Characterization of the Interaction between Prefoldin and Group II Chaperonin (2010)
  • Author(s): Sahlan, M., Zako, T., Tai, P. T., Ohtaki, A., Noguchi, K., Maeda, M., Miyatake, H., Dohmae, N., Yohda, M.
  • Journal Title: J. Mol. Biol Volume: 399 Pages: 628-636
  • Peer Reviewed
• [Journal Article] Analysis of the interaction mode between hyperthermophilic archaeal group II chaperonin and prefoldin using a platform of chaperonin oligomers of various subunit arrangements (2010)
  • Author(s): Sahlan, M., Kanzaki, T., Zako, T., Maeda, M., Yohda, M.
  • Journal Title: Biochim. Biophys. Acta Volume: 1804 Pages: 1810-1816
  • Peer Reviewed
• [Journal Article] Adaptation of a hyperthermophilic group II chaperonin to relatively moderate temperatures (2010)
  • Author(s): Kanzaki, T., Ushioku, S., Nakagawa, A., Oka, T., Takahashi, K., Nakamura, T., Kuwajima, K., Yamagishi, A., Yohda, M.
  • Journal Title: Protein Eng. Des. Se Volume: 23 Pages: 393-402
  • Peer Reviewed
• [Journal Article] 磁性細菌Magnetospirillum magneticum AMB-1由来sHspの機能と構造 (2010)
  • Author(s): 阿部哲也、伊藤慎平、西直哉、塚田祥弘、安永卓生、新垣篤史、松永是、養王田正文
  • Journal Title: 高分子論文集 Volume: 67 Pages: 698-704
  • NAID: 10027624299
  • Peer Reviewed
• [Journal Article] Biophysical characterization of highly active recombinant Gaussia luciferase expressed in Escherichia coll. (2010)
  • Author(s): Rathnayaka T, Tawa M, Sohya S, Yohda M, Kuroda Y.
  • Journal Title: Biochim Biophys Acta. Volume: 1804 Pages: 1902-1907
  • Peer Reviewed
• [Journal Article] Thermodynamic characterization of the interaction between prefoldin and group II chaperonin. (2010)
  • Author(s): Sahlan M, Zako T, Tai PT, Ohtaki A, Noguchi K, Maeda M,Miyatake H, Dohmae N, Yohda M.
  • Journal Title: J Mol Biol. Volume: 399 Pages: 628-636
  • Peer Reviewed
• [Journal Article] Analysis of the interaction mode between hyperthermophilic archaeal group II chaperonin and prefoldin using a platform of chaperonin oligomers of various subunit arrangements. (2010)
  • Author(s): Sahlan M, Kanzaki T, Zako T, Maeda M, Yohda M.
  • Journal Title: Biochim Biophys Acta. Volume: 1804 Pages: 1810-1816
  • Peer Reviewed
• [Journal Article] Crystal structure of 1-deoxy-d-xylulose 5-phosphate reductoisomerase from the hyperthermophile Thermotoga maritima for insights into the coordination of conformational changes and an inhibitor binding. (2010)
  • Author(s): Takenoya M, Ohtaki A, Noguchi K , Endo K, Sasaki Y, Ohsawa K, Yajima S, Yohda M.
  • Journal Title: J Struct Biol. Volume: 170 Pages: 532-539
  • Peer Reviewed
• [Journal Article] Kinetic and structural studies on roles of the serine ligand and a strictly conserved tyrosine residue in nitrile hydratase. (2010)
  • Author(s): Yamanaka Y, Hashimoto K, Ohtaki A, Noguchi K, Yohda M, Odaka M.
  • Journal Title: J Biol Inorg Chem. Volume: 15 Pages: 655-665
  • Peer Reviewed
• [Journal Article] Adaptation of a hyperthermophilic group II chaperonin to relatively moderate temperatures. (2010)
  • Author(s): Kanzaki T, Ushioku S, Nakagawa A, Oka T, Takahashi K, Nakamura T, Kuwajima K, Yamagishi A, Yohda M.
  • Journal Title: Protein Eng Des Sel. 23 Pages: 393-402
  • Peer Reviewed
• [Journal Article] Structure and function of archaeal prefoldin, a co-chaperone of group II chaperonin. (2010)
  • Author(s): Ohtaki A, Noguchi K, Yohda M.
  • Journal Title: Front Biosci. 15 Pages: 708-717
  • Peer Reviewed
• [Journal Article] Crystal structures of the lumazine protein from Photobacterium kishitanii in complexes with the authentic chromophore, 6,7-dimethyl-8-(1'-D-ribityl) lumazine, and its analogues, riboflavin and flavin mononucleotide, at high resolution. (2010)
  • Author(s): Sato Y, Shimizu S, Ohtaki A, Noguchi K, Miyatake H, Dohmae N, Sasaki S, Odaka M, Yohda M.
  • Journal Title: J Bacteriol. 192 Pages: 127-133
  • Peer Reviewed
• [Journal Article] Hyperthermophilic archaeal prefoldin shows refolding activity at low temperature. (2010)
  • Author(s): Zako T, Banba S, Sahlan M, Sakono M, Terada N, Yohda M, Maeda M.
  • Journal Title: Biochem Biophys Res Commun. 391 Pages: 467-470
  • Peer Reviewed
• [Journal Article] Characterization of a sHsp of Schizosaccharomyces pombe, SpHsp 15. 8, and the implication of its functional mechanism by comparison with another sHsp (2009)
  • Author(s): Sugino, C., Hirose, M., Tohda, H., Yoshinari, Y., Abe, T., Giga-Hama, Y., Iizuka, R., Shimizu, M., Kidokoro, S., Ishii, N., Yohda, M.
  • Journal Title: Proteins Volume: 74 Pages: 6-17
  • Peer Reviewed
• [Journal Article] Crystallization and heavy-atom derivatization of StHsp14.0, a small heat-shock protein from Sulfolobus tokodaii. (2009)
  • Author(s): Hayashi T, Abe T, Takeda K, Akiyama N, Yohda M, Miki K.
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun. 65(Pt 10) Pages: 1007-1010
  • Peer Reviewed
• [Journal Article] Structural basis for catalytic activation of thiocyanate hydrolase involving metal-ligated cysteine modification. (2009)
  • Author(s): Arakawa T, Kawano Y, Katayama Y, Nakayama H, Dohmae N, Yohda M, Odaka M.
  • Journal Title: J Am Chem Soc. 131 Pages: 14838-14843
  • Peer Reviewed
• [Journal Article] Construction and characterization of the hetero-oligomer of the group II chaperonin from the hyperthermophilic archaeon, Thermococcus sp. strain KS-1. (2009)
  • Author(s): Sahlan M, Kanzaki T, Yohda M.
  • Journal Title: Extremophiles 13 Pages: 437-445
  • Peer Reviewed
• [Journal Article] Structural studies on the oligomeric transition of a small heat shock protein
  • Author(s): Hanazono, Y., Takeda, K., Yohda, M., Miki, K.
  • Journal Title: Mol. Biol. in press Volume: 14
• [Presentation] 循環置換型連結変異体によるグループII型シャペロニンヘテロリング複合体の構築 (2011)
  • Author(s): 山本陽平、阿部由寛、守谷和騎、阿部哲也、養王田正文
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] 超好熱性古細菌由来II型シャペロニンのリング内サブユニット間協調作用機構に関する研究 (2011)
  • Author(s): 有田真優乃、守谷和騎、真壁幸樹、桑島邦博、養王田正文
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] ATP dependent conformational change and protein folding mechanism of group II chaperonin (2011)
  • Author(s): Yohda M, Nakagawa A, Moriya K, Oka T, Sekiguchi H, Sasaki YC, Makabe K, Kuwajima K
  • Organizer: The 4th UP International Symposium
  • Place of Presentation: ローマ大学、イタリア(招待講演)
  • Year and Date: 2011-11-29
• [Presentation] 超好熱性古細菌Thermococcus strain KS-1由来グループII型シャペロニンの反応機構 (2011)
  • Author(s): 養王田正文、中川あゆみ、守谷和騎、岡俊彦、関口博史、佐々木裕次、真壁幸樹、桑島邦博
  • Organizer: 第6回臨床ストレス応答学会大会
  • Place of Presentation: 名古屋大学舞鶴キャンパス
  • Year and Date: 2011-11-04
• [Presentation] ATP dependent conformational change and protein folding mechanism of group II chaperonin (2011)
  • Author(s): M. Yohda, A. Nakagawa, K. Moriya, T. Oka, H. Sekiguchi, Y. C. Sasaki, K. Makabe, K.
  • Organizer: The 11th KIAS Conference on Protein Structure and Function, KIAS(KOREA INSTITUTE FOR ADVANCED STUDY) Seoul
  • Place of Presentation: South Korea
  • Year and Date: 2011-10-08
• [Presentation] ATP dependent conformational change and protein folding mechanism of group II chaperonin (2011)
  • Author(s): Yohda M, Nakagawa A, Moriya K, Oka T, Sekiguchi H, Sasaki YC, Makabe K, Kuwajima K
  • Organizer: The 11th KIAS Conference on Protein Structure and Function
  • Place of Presentation: KOREA INSTITUTE FOR ADVANCED STUDY、韓国(招待講演)
  • Year and Date: 2011-10-08
• [Presentation] 超好熱性古細菌Thermococcus strain KS-1 由来グループII型シャペロニンの反応機構 (2011)
  • Author(s): 養王田正文、中川あゆみ、守谷和騎、岡俊彦、関口博史、佐々木裕次、真壁幸樹、桑島邦博
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大学小金井キャンパス
  • Year and Date: 2011-09-27
• [Presentation] 超好熱性古細菌由来II型シャペロニンのATP結合・加水分解による構造変化機構に関する研究 (2011)
  • Author(s): 有田真優乃、北村享太郎、守谷和騎、中川あゆみ、真壁幸樹、桑島邦博、養王田正文
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大学小金井キャンパス
  • Year and Date: 2011-09-27
• [Presentation] 循環置換型連結変異体によるII型シャペロニンヘテロリング複合体の構築 (2011)
  • Author(s): 山本陽平、阿部由寛、阿部哲也、養王田正文
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大学小金井キャンパス
  • Year and Date: 2011-09-27
• [Presentation] 超好熱性古細菌由来II型シャペロニンのヌクレオチド結合部位の構造変化に関する研究 (2011)
  • Author(s): 守谷和騎、北村享太郎、中川あゆみ、真壁幸樹、桑島邦博、養王田正文
  • Organizer: 第11回蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク、大阪
  • Year and Date: 2011-06-07
• [Presentation] 循環置換型連結変異体によるII型シャペロニンヘテロリング複合体の構築 (2011)
  • Author(s): 山本陽平、阿部由寛、阿部哲也、養王田正文
  • Organizer: 第11回蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク、大阪
  • Year and Date: 2011-06-07
• [Presentation] 速度論的解析に基づくグループII型シャペロニンの反応機構 (2011)
  • Author(s): 養王田正文、中川あゆみ、守谷和騎、岡俊彦、真壁幸樹、桑島邦博
  • Organizer: 第11回蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク、大阪
  • Year and Date: 2011-06-07
• [Presentation] Conformational change and protein folding mechanism of group II chaperonin proposed from stopped-flow experiments and diffracted X-ray tracking (2011)
  • Author(s): M. Yohda, A. Nakagawa, H. Sekiguchi, K. Moriya, T. Oka, K. Makabe, K. Kuwajima,. Y. C.
  • Organizer: The 3rd Pacific Protein Association Conference in conjunction with the 3rd Symposium of the Chinese Protein Society
  • Place of Presentation: Shanghai University
  • Year and Date: 2011-05-06
• [Presentation] Conformational change and protein folding mechanism of group II chaperonin proposed from stopped-flow experiments and diffracted X-ray tracking (2011)
  • Author(s): Yohda M, Nakagawa A, Sekiguchi H, Moriya K, Oka T, Makabe K, Kuwajima K, Sasaki YC
  • Organizer: The 3rd Pacific Protein Association Conference
  • Place of Presentation: 上海大学、中国
  • Year and Date: 2011-05-06
• [Presentation] Structure, function and application of hyperthirmophilic molecular chaperonins (2010)
  • Author(s): M. Yohda, A. Nakagawa, T. Kanzaki, H. Sekiguchi, Y. C.
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies(Pacifichem 2010), Hawaii Convention Center
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-19
• [Presentation] Mechanism of protein folding by prefoldin and group II chaperonin system (2010)
  • Author(s): Masafumi Yohda
  • Organizer: The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering
  • Place of Presentation: Chengdu, China 招待講演
  • Year and Date: 2010-11-07
• [Presentation] Mechanism of protein folding by prefoldin and group II chaperonin system (2010)
  • Author(s): M. Yohda
  • Organizer: 11th China-Japan-Korea Joint Symposium on Enzyme Engineering
  • Place of Presentation: Chengdu, China
  • Year and Date: 2010-11-05
• [Presentation] Structure, function and application of hyperthrmophilic molecular chaperones (2010)
  • Author(s): Masafumi Yohda, Ayumi Nakagawa, Taro Kanzaki, Hiroshi Sekiguchi, Yuji C.Sasaki
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2010-10-19
• [Presentation] Thermodynamic Characterization of the Interaction between Prefoldin and Group II Chaperonin (2010)
  • Author(s): Muhamad Sahlan, Tamotsu Zako, Phan The Tai, Akashi Ohtaki, Keiichi Noguchi, Hideyuki Miyatake, Naoshi Dohmae, Mizuo Maeda, Masafumi Yohda
  • Organizer: International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良、奈良
  • Year and Date: 2010-09-13
• [Presentation] Characterization of Heat Shock Protein 90 and Its Co-chaperones from Schizosaccharomyces pombe (2010)
  • Author(s): Tetsuya Abe, Taichi Tomonari, Mari Ishida, Taro Kanzaki, Toshihiko Oka, Masafumi Yohda
  • Organizer: International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良、奈良
  • Year and Date: 2010-09-13
• [Presentation] ATP-induced Dynamic Motion of Group II Chaperonin Detectedby Diffracted X-ray Tracking (2010)
  • Author(s): Ayumi Nakagawa, Kazuki Moriya, Hiroshi Sekiguchi, Yuji Sasaki, Masafumi Yohda
  • Organizer: International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良、奈良
  • Year and Date: 2010-09-13
• [Presentation] Thermococcus strain KS-1由来グループII型シャペロニンのステムループ領域の機能 (2010)
  • Author(s): 守谷和騎、中川あゆみ、神前太郎、養王田正文
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2010-06-18
• [Presentation] 分裂酵母Schizosaccharomyces pombe 由来Hsp90とコシャペロンの協調作用機構 (2010)
  • Author(s): 友成太一、石田眞理、阿部哲也、神前太郎、養王田正文
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2010-06-18
• [Presentation] Role of the C terminal region of the group II chaperonin in the interaction with PFD (2010)
  • Author(s): Phan The Tai、Muhamad Sahlan、養王田正文
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2010-06-18
• [Presentation] 超好熱性古細菌由来II型シャペロニンの反応機構の速度論的解析 (2010)
  • Author(s): 中川あゆみ、神前太郎、守谷和騎、五十嵐圭日子、桑島邦博、養王田正文
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2010-06-18
• [Presentation] 分子シャペロンをめぐる分子間相互作用の解析 (2010)
  • Author(s): 養王田正文、阿部哲也、Muhamad Sahlan、 Phan The Tai、神前太郎、岡俊彦、座古保、前田端夫
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター、札幌 招待講演
  • Year and Date: 2010-06-18
• [Presentation] Substrate Binding Analysis of Group II Chaperonin using Surface Plasmon Resonance (2009)
  • Author(s): Neng Risma Liasari、muhamad Sahlan、座古保、前田瑞夫、養王田正文
  • Organizer: 第32回分子生物学会年会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Characterization of the interaction between chaperonin and prefoldin at the high temperature (2009)
  • Author(s): The Tai Phan、Muhamad Sahlan、Tamotsu Zako、Mizuo Maeda、Masafumi Yohda
  • Organizer: 第32回分子生物学会年会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] 超好熱性古細菌thermococcus strain KS-1由来プレフォルディンの基質認識機構 (2009)
  • Author(s): 大滝証、菅野由利、佐藤孝晴、野口恵一、尾高雅文、養王田正文
  • Organizer: 第82回日雄化学会大会
  • Place of Presentation: 神戸・神戸ポートアイランド
  • Year and Date: 2009-10-21
• [Presentation] Thermodynamic properties of interaction between prefoldin and group II chaperonin (2009)
  • Author(s): Muhamad Sahlan、Phan The Tai、Tomatsu Zako、Motonori Kudo、Kohei Tsumoto、Mizuo Maeda、Masafumi Yohda
  • Organizer: The 4th Annual Meeting of the Biomedical Society for Stress Response
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-09
• [Presentation] Kinetic study on the reaction mechanism of a group II chaperonin by stopped flow experiment (2009)
  • Author(s): Ayumi Nakagawa、Taro Kanzaki、Rie Masuda、Kiyohiko Igarashi、Masafumi Yohda
  • Organizer: The 4th Annual Meeting of the Biomedical Society for Stress Response
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-09
• [Presentation] Conformational change mechanism of group II chaperonin (2009)
  • Author(s): M. Yohda, T. Kanzaki, R. Masuda, A. Namagawa, K.
  • Organizer: 21th IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Shanghai International Conventional Center
  • Year and Date: 2009-08-04
• [Presentation] Conformational change mechnism to group II chaperonin (2009)
  • Author(s): Yohda M., Kanzaki T., Masuda R., Nakagawa A., Igarashi K.
  • Organizer: 21th IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 上海、中国
  • Year and Date: 2009-08-02
• [Presentation] Conformational change mechanism of group II chaperonin (2009)
  • Author(s): Masafumi Yohda、Taro Kanzaki、Rie Masuda、Ayumi Namagawa、Kiyohiko Igarashi
  • Organizer: 21th IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 上海、中国
  • Year and Date: 2009-08-02
• [Presentation] Cold-adaptation of a hyperthennophilic group II chaperonin (2009)
  • Author(s): Taro Kanzaki、Ayumi Nakagawa、Shuzo Ushioku、Toshihiko Oka、Kazunobu Takahashi、Takashi Nakamura、Kunihiro Kuwajima、Akihiko Yamagishi, Masafumi
  • Organizer: 34th FEBS Congress
  • Place of Presentation: チェコ
  • Year and Date: 2009-07-04
• [Presentation] 超好熱性古細菌由来II型シャペロニン低温適応化変異体の解析 (2009)
  • Author(s): 中川あゆみ、神前太郎、牛奥修三、高橋一暢、中村敬、岡俊彦、桑島邦博、山岸明彦、養王田正文
  • Organizer: 第12回マリンバイオテクノロジー学会大会
  • Place of Presentation: 東京・早稲田大学
  • Year and Date: 2009-05-30
• [Presentation] 超好熱性古細菌thermococcus strain KS-1由来プレフォルディンの基質認識機構の検討 (2009)
  • Author(s): 大滝証、野口恵一、菅野由利、佐藤孝晴、尾高雅文、養王田正文
  • Organizer: 第12回マリンバイオテクノロジー学会大会
  • Place of Presentation: 東京・早稲田大学
  • Year and Date: 2009-05-30
• [Presentation] 分裂酵母由来グループII型シャペロニンおよびプレフォルディンの大量発現・精製系の構築 (2009)
  • Author(s): 石田眞理, 神前太郎, 増田莉恵, 東田英毅, 養王田正文
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本・熊本全日空ホテル
  • Year and Date: 2009-05-20
• [Presentation] 超好熱性古細菌Thermococcus.Strain KS-1由来プレフォルディンとシャペロニンの強調作用機構の解析 (2009)
  • Author(s): 米澤哲洋、大滝証、野口恵一、養王田正文
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本・熊本全日空ホテル
  • Year and Date: 2009-05-20
• [Presentation] Rebuilt Group II Chaperonin Hetero-oligomers to Reveals interaction Mode Betwees Group II Chaperonin and Prefoldin (2009)
  • Author(s): Sahlan Muhamad、Kanzaki Tato、Zako Tomotsu、Maeda Mizuo、Yohda Masafumi
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本・熊本全日空ホテル
  • Year and Date: 2009-05-20
• [Presentation] Mechanism in Conformational Change of Group II Chaperonin EXPERIMENTAL BIOLOGY 2009(ASBMB) (2009)
  • Author(s): M. Yohda、T. Kanzaki、R. Masuda、S. Muhamad、T.
  • Organizer: Ernest N. Morial Convention Center
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-04-21
• [Presentation] Mechanism in Conformational Change of Group II Chaperonin (2009)
  • Author(s): Yohda M., Kanzaki T., Masuda R., Sahlan M., Nakagawa A., Oka T., Takahashi K., Maki K., Yebenes H., Valpuesta M., Igarashi K., Kuwajima K.
  • Organizer: EXPERIMENTAL BIOLOGY 2009
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-04-20
• [Presentation] Chaperone Mechines and Celluler Protein Folding (2009)
  • Author(s): M.Yohda, T.Kanzaki, R.Masuda, S.Muhamad, T.Oka
  • Organizer: EXPERIMENTAL BIOLOGY 2009
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-04-20
• [Remarks]
  • URL: http://www.tuat.ac.jp/~yohda/index.html