Molecular mechanism of the desiccation tolerance induced by trehalose and LEA proteins in anhydrobiotic organisms

• Project/Area Number: 21370068
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: SAKURAI Minoru 東京工業大学, バイオ研究基盤支援総合センター, 教授 (50162342)
• Co-Investigator(Renkei-kenkyūsha): KIKAWADA Takahiro 独立行政法人農業生物資源研究所, 乾燥耐性研究ユニット, 主任研究員 (60414900)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: タンパク質 / 核酸の構造 / 動態 / 機能 / LEAタンパク質 / トレハロース / 乾燥耐性 / 水 / 細胞膜 / 巨大リポソーム / 凝集抑制 / 幹燥耐性 / ガラス状態

Research Abstract

We have revealed some of the molecular mechanisms responsible for the desiccation tolerance of anhydrobiotic organisms such as Polypedilum vanderplanki. Fisrt is the structuralization of LEA proteins in the dry state and their vitrification with trehalose(steel-reinforced concrete model). Second is the ion-scavenger effect of LEA proteins and their anti-aggregation effect against on proteins during desiccation. Furthermore, we have obtained the invaluable information about the substrate transport mechanism of trehalose transporter.

Research Products

• [Journal Article] トレハロースと水の相互作用が織りなす細胞保護機能 (2012)
  • Author(s): 櫻井実
  • Journal Title: 低温生物工学会誌 Volume: 57 Pages: 41-51
  • Peer Reviewed
• [Journal Article] 分子動力学シミュレーションを用いたGroup3LEAペプチドのタンパク質凝集抑制機能の解析 (2011)
  • Author(s): 山川賢太郎, 古木隆生, 櫻井実
  • Journal Title: 低温生物工学会誌 Volume: 57 Pages: 143-146
  • NAID: 10030865619
  • Peer Reviewed
• [Journal Article] グループ3LEAタンパク質の22-merモデルペプチドが示すタンパク質凝集抑制効果 (2011)
  • Author(s): 古木隆生, 清水天平, S. Charkrabortee, 畑中理恵, 高橋剛, 黄川田隆洋, 奥田隆, 三原久和, A. Tunnacliffe, 櫻井実
  • Journal Title: 低温生物工学会誌 Volume: 57 Pages: 139-142
  • NAID: 110009457797
  • Peer Reviewed
• [Journal Article] ネムリユスリカ由来の細胞保護タンパク質の機能と低分子ペプチドによる代替 (2011)
  • Author(s): 畑中理恵, 古木隆生, 櫻井実, 黄川田隆洋
  • Journal Title: BIO INDUSTRY Volume: 28 Pages: 43-48
• [Journal Article] Experimental and theoretical study on the intermolecular complex formation between trehalose and benzene compounds in aqueous solution (2011)
  • Author(s): K. Sakakura, A. Okabe, K. Oku, M. Sakurai
  • Journal Title: J. Phys. Chem. B Volume: 11 Pages: 9823-9830
  • Peer Reviewed
• [Journal Article] Salt Effects on the Structural and Thermodynamic Properties of a Group 3 LEA Protein Model Peptide (2011)
  • Author(s): T. Furuki, T. Shimizu, T. Kikawada, T. Okuda, M. Sakurai
  • Journal Title: Biochemistry Volume: 50 Pages: 7093-7103
  • Peer Reviewed
• [Journal Article] Experimental and Theoretical Study on the Intermolecular Complex Formation Between Trehalose and Benzene Compounds (2011)
  • Author(s): K. Sakakura
  • Journal Title: J. Phys. Chem. B Volume: 115 Issue: 32 Pages: 9823-9830
  • DOI: 10.1021/jp2037203
  • Peer Reviewed
• [Journal Article] Computer simulation on interactions between trehalose and diene in aqueous solution (2010)
  • Author(s): K. Sakakura, K. Oku, M. Sakurai
  • Journal Title: Cryobiol. Cryotech. Volume: 56 Pages: 173-178
  • NAID: 110008662307
  • Peer Reviewed
• [Journal Article] Model Study of the Desiccation-induced Vitrification of Group-3 Late Embrogenesis Abundant Proteins (2010)
  • Author(s): T. Furuki, T. Shimizu, T. Takahashi, H. Mihara, T. Kikawada, T. Okuda, M. Sakurai
  • Journal Title: Cryobiol. Cryotech. Volume: 56 Pages: 51-54
  • NAID: 110008662281
  • Peer Reviewed
• [Journal Article] Structural Analysis for Dehydrated LEA Proteins of Polypedilum vanderplanki by Replica Exchange Molecular Dynamics Simulation (2010)
  • Author(s): S. Miyama, T. Shimizu, Y. Kanamori, T. Furuki, T. Kikawada, T. Okuda, M. Sakurai
  • Journal Title: Cryobiol. Cryotech. Volume: 56 Pages: 27-30
  • NAID: 110008662287
  • Peer Reviewed
• [Journal Article] Desiccation-Induced Structuralization and Glass Formation of Group 3 Late Embryogenesis Abundant Protein Model Peptides (2010)
  • Author(s): T. Shimizu, Y. Kanamori, T. Furuki, T. Kikawada, T. Okuda, T. Takahashi, H. Mihara, M. Sakurai
  • Journal Title: Biochemistry Volume: 49 Pages: 1093-1104
  • Peer Reviewed
• [Journal Article] Computer simulation on interactions between trehalose and diene in aqueous solution (2010)
  • Author(s): Sakakura, K., Oku, K., Sakurai, M.
  • Journal Title: Cryobiol.Cryotech. Volume: 56 Pages: 173-178
  • NAID: 110008662307
  • Peer Reviewed
• [Journal Article] Desiccation-Induced Structuralization and Glass Formation of Group 3 Late Embryogenesis Abundant Protein Model Peptides (2010)
  • Author(s): Shimizu, T., Kanamori, Y., Furuki, T., Kikawada, T., Okuda, T., Takahashi, T., Mihara, H., Sakurai, M.
  • Journal Title: Biochemistry 49 Pages: 1093-1104
  • Peer Reviewed
• [Journal Article] Structural Analysis for Dehydrated LEA Proteins of Polypedilum vanderplanki by Replica Exchange Molecular Dynamics Simulation (2010)
  • Author(s): Miyama, S., Shimizu, T., Kanamori, Y., Furuki, T., Kikawada, T., Okuda, T., Sakurai, M.
  • Journal Title: Cryobiol.Cryotech. 56 Pages: 27-30
  • NAID: 110008662287
  • Peer Reviewed
• [Journal Article] Model Study of the Desiccation-induced Vitrification of Group-3 Late Embrogenesis Abundant Proteins (2010)
  • Author(s): Furuki, T.Shimizu, T., Takahashi, T., Mihara, H., Kikawada, T., Okuda, T., Sakurai, M.
  • Journal Title: Cryobiol.Cryotech. 56 Pages: 51-54
  • NAID: 110008662281
  • Peer Reviewed
• [Presentation] 計算機シミュレーションから分かったトレハロースの水和特性とトレハロース-タンパク質間相互作用の新しい描像 (2011)
  • Author(s): 櫻井実
  • Organizer: トレハロースシンポジウム
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-11-04
• [Presentation] LEAタンパク質のモデルペプチドが示す分子構造及びガラス転移の含水率依存性 (2011)
  • Author(s): 古木隆生,清水天平,黄川田隆洋,奥田隆,櫻井実
  • Organizer: 第37回熱測定討論会
  • Place of Presentation: 桐生
• [Presentation] トレハロースと水の相互作用が織りなす細胞保護機能 (2011)
  • Author(s): 櫻井実
  • Organizer: 第56回低温生物工学会年会
  • Place of Presentation: 盛岡
• [Presentation] グループ3LEAタンパク質の22merモデルペプチドが示すタンパク質凝集抑制効果 (2011)
  • Author(s): 山川賢太郎,古木隆生,櫻井実
  • Organizer: 第56回低温生物工学会年会
  • Place of Presentation: 盛岡
• [Presentation] Effects of group 3 LEA model peptides on desiccation-induced protein aggregation (2011)
  • Author(s): T. Furuki, T. Shimizu, S. Chakrabortee, R. Hatanaka, T. Kikawada, T. Okuda, A. Tunnacliffe, M. Sakurai
  • Organizer: 4th International Symposiun on the Environmental Physiology of Ectotherms & Plants
  • Place of Presentation: Rennes, France
• [Presentation] Effects of group 3 LEA model peptides on desiccation-induced protein aggregation (2011)
  • Author(s): T.Furuki, T.Shimizu, S.Chakrabortee, R.Hatanaka, T.Kikawada, T.Okuda, A.Tunnacliffe, M.Sakurai
  • Organizer: 4th International Symposiun on the Environmental Physiology of Ectotherms & Plants
  • Place of Presentation: Rennes、France
• [Presentation] 分子動力学シミュレーションを用いたGroup3LEAペプチドのタンパク質凝集抑制機能の解析 (2011)
  • Author(s): 山川賢太郎, 古木隆生, 櫻井実
  • Organizer: 第56回低温生物工学会年会
  • Place of Presentation: 盛岡
• [Presentation] トレハロースと水の相互作用が織りなす細胞保護機能 (2011)
  • Author(s): 櫻井実
  • Organizer: 第56回低温生物工学会年会
  • Place of Presentation: 盛岡(招待講演)
• [Presentation] 水溶液中におけるトレハロースとジエンの相互作用に関する計算機シミュレーション (2010)
  • Author(s): 坂倉耕太,奥和之,櫻井実
  • Organizer: 第55回低温生物工学会
  • Place of Presentation: 東京工業大学
  • Year and Date: 2010-06-26
• [Presentation] Molecular dynamics simulation study on trehalose-benzene interactions in aqueous solution (2010)
  • Author(s): A. Okabe, K. Oku, K. Sakakura, S. Fukuda, and M. Sakurai
  • Organizer: Pacifichem 2010 Congress
  • Place of Presentation: ホノルル
• [Presentation] モデルペプチドを用いたLEA(Late Embryogenesis Abundant)タンパク質の分子構造とガラス化に与える塩濃縮効果の検討 (2010)
  • Author(s): 古木隆生,清水天平,黄川田隆洋,奥田隆,高橋剛,三原久和,櫻井実
  • Organizer: 第46回熱測定討論会
  • Place of Presentation: 三重大学
• [Presentation] モデルペプチドを用いたLEA (Late Embryogenesis Abundant)タンパク質の分子構造とガラス化に与える塩濃縮効果の検討 (2010)
  • Author(s): 古木隆生、清水天平、黄川田隆洋、奥田隆、高橋剛、三原久和、櫻井実
  • Organizer: 第46回熱測定討論会
  • Place of Presentation: 三重大学
• [Presentation] トレハロースの水和構造と抗酸化作用に関する計算機シミュレーション (2009)
  • Author(s): 坂倉耕太、櫻井実
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティ徳島(徳島)
  • Year and Date: 2009-11-01
• [Presentation] Model Study of the Desiccation-induced Vitrification of Group-3 Late Embryogenesis Abundant Proteins (2009)
  • Author(s): T.Furuki, T.Shimizu, T.Kikawada, T.Okuda, T.Takahashi, H.Mihara, M.Sakurai
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 筑波 国際会議場
  • Year and Date: 2009-08-26
• [Presentation] Structural analysis for dehydrated LEA proteins of Polypedilum vanderplanki by replica exchange molecular dynamics simulation (2009)
  • Author(s): S.Miyama, T.Shimizu, T.Furuki, Y.Harano, Y.Kanamori, T.Kikawada, T.Okuda, M.Sakurai
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 筑波 国際会議場
  • Year and Date: 2009-08-24
• [Presentation] Three-dimensional structure and dynamics of trehalose transporter TRET1 in Polypedilum vandeplanki as revealed by computer simulations (2009)
  • Author(s): T.Okawa, T.Kikawada, Okuda, M.Sakurai
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 筑波 国際会議場
  • Year and Date: 2009-08-24
• [Presentation] Salt Effects on the Conformational Structure of LEA Protein from Polypedilum vanderplanki and of Its Model Peptide. (2009)
  • Author(s): T.Furuki, .Shimizu, M.Miyazawa, T.Kikawada, T.Okuda, M.Sakurai
  • Organizer: 3rd International Symposium on the Environmental Physiology of Ectotherms and Plants
  • Place of Presentation: 筑波 国際会議場
  • Year and Date: 2009-08-24
• [Presentation] Three-dimensional structure and dynamics of trehalose transporter TRET1 in Polypedilum vandeplanki as revealed by computer simulations (2009)
  • Author(s): T. Okawa, T. Kikawada,. Okuda, M. Sakurai
  • Organizer: CRYO 2009(46^<th> Annual meeting of the society for cryobiology)
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-22
• [Presentation] Model Study of the Desiccation-induced Vitrification of Group-3 Late Embryogenesis Abundant Proteins (2009)
  • Author(s): T. Furuki, T. Shimizu, T. Kikawada, T. Okuda, T. Takahashi, H. Mihara, and M. Sakurai
  • Organizer: CRYO 2009(46^<th> Annual meeting of the society for cryobiology)
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-22
• [Presentation] Salt Effects on the Conformational Structure of LEA Protein from Polypedilum vanderplanki and of Its Model Peptide (2009)
  • Author(s): T. Furuki, T. Shimizu, M. Miyazawa, T. Kikawada, T. Okuda, M. Sakurai
  • Organizer: CRYO 2009(46^<th> Annual meeting of the society for cryobiology)
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-22
• [Presentation] Salt Effects on the Conformational Structure of LEA Protein from Polypedilum vanderplanki and of Its Model Peptide. (2009)
  • Author(s): T.Furuki, T.Shimizu, M.Miyazawa, T.Kikawada, T.Okuda, M.Sakurai
  • Organizer: CRYO 2009 (46^<th> Annual meeting of the society for cryobiology)
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-22
• [Presentation] Model Study of the Desiccation-induced Vitrification of Group-3 Late Embryogenesis Abundant Proteins (2009)
  • Author(s): T.Furuki, T.Shimizu, T.Kikawada, T.Okuda, T.Takahashi, H.Mihara, M.Sakurai
  • Organizer: CRYO 2009 (46^<th> Annual meeting of the society for cryobiology)
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-20
• [Presentation] Three-dimensional structure and dynamics of trehalose transporter TRET1 in Polypedilum vandeplanki as revealed by computer simulations (2009)
  • Author(s): T.Okawa, T.Kikawada, .Okuda, M.Sakurai
  • Organizer: CRYO 2009 (46^<th> Annual meeting of the society for cryobiology)
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-20
• [Presentation] Structural analysis for dehydrated LEA proteins of Polypedilum vanderplanki by replica exchange molecular dynamics simulation (2009)
  • Author(s): S. Miyama, T. Shimizu, T. Furuki, Y. Harano, Y. Kanamori, T. Kikawada, T. Okuda and M. Sakurai
  • Organizer: CRYO 2009(46^<th> Annual meeting of the society for cryobiology)
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-19
• [Presentation] Structural analysis for dehydrated LEA proteins of Polypedilum vanderplanki by replica exchange molecular dynamics simulation (2009)
  • Author(s): S.Miyama, T.Shimizu, T.Furuki, Y.Harano, Y.Kanamori, T.Kikawada, T.Okuda, M.Sakurai
  • Organizer: CRYO 2009 (46^<th> Annual meeting of the society for cryobiology)
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-07-19
• [Presentation] レプリカ交換MDシミュレーションによるLEA蛋白質由来22残基ポリペプチドの構造解析 (2009)
  • Author(s): 三山祥平,清水天平,原野雄一,櫻井実
  • Organizer: 第9回日本蛋白質科学会
  • Place of Presentation: 熊本全日空ホテル
  • Year and Date: 2009-05-21