Molecular mechanism of the desiccation tolerance induced by trehalose and LEA proteins in anhydrobiotic organisms
| Project/Area Number |
21370068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SAKURAI Minoru 東京工業大学, バイオ研究基盤支援総合センター, 教授 (50162342)
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| Co-Investigator(Renkei-kenkyūsha) |
KIKAWADA Takahiro 独立行政法人農業生物資源研究所, 乾燥耐性研究ユニット, 主任研究員 (60414900)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | タンパク質 / 核酸の構造 / 動態 / 機能 / LEAタンパク質 / トレハロース / 乾燥耐性 / 水 / 細胞膜 / 巨大リポソーム / 凝集抑制 / 幹燥耐性 / ガラス状態 |
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| Research Abstract |
We have revealed some of the molecular mechanisms responsible for the desiccation tolerance of anhydrobiotic organisms such as Polypedilum vanderplanki. Fisrt is the structuralization of LEA proteins in the dry state and their vitrification with trehalose(steel-reinforced concrete model). Second is the ion-scavenger effect of LEA proteins and their anti-aggregation effect against on proteins during desiccation. Furthermore, we have obtained the invaluable information about the substrate transport mechanism of trehalose transporter.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Desiccation-Induced Structuralization and Glass Formation of Group 3 Late Embryogenesis Abundant Protein Model Peptides2010
Author(s)
Shimizu, T., Kanamori, Y., Furuki, T., Kikawada, T., Okuda, T., Takahashi, T., Mihara, H., Sakurai, M.
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Journal Title
Biochemistry 49
Pages: 1093-1104
Related Report
Peer Reviewed
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[Journal Article] Structural Analysis for Dehydrated LEA Proteins of Polypedilum vanderplanki by Replica Exchange Molecular Dynamics Simulation2010
Author(s)
Miyama, S., Shimizu, T., Kanamori, Y., Furuki, T., Kikawada, T., Okuda, T., Sakurai, M.
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Journal Title
Cryobiol.Cryotech. 56
Pages: 27-30
NAID
Related Report
Peer Reviewed
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[Journal Article] Model Study of the Desiccation-induced Vitrification of Group-3 Late Embrogenesis Abundant Proteins2010
Author(s)
Furuki, T.Shimizu, T., Takahashi, T., Mihara, H., Kikawada, T., Okuda, T., Sakurai, M.
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Journal Title
Cryobiol.Cryotech. 56
Pages: 51-54
NAID
Related Report
Peer Reviewed
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