Function of the photo-induced triplet radical pair in the photo-sensor BLUF protein
| Project/Area Number |
21370069
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
MINO Hiroyuki 名古屋大学, 理学研究科, 准教授 (70300902)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Takaaki 茨城大学, 工学部, 教授 (10175268)
MASUDA Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | 光生物 / BLUF / 磁場効果 / EPR / ESR / フラビン / センサータンパク質 / ラジカル対 / 時間分解EPR / 時間分EPR / BLUP |
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| Research Abstract |
A photo-induced radical pair of FADH・and Y8・in BLUF protein SyPixD was studied by electron paramagnetic resonance(EPR) spectroscopy. The EPR signal has been characterized by a Pake doublet signal with complete S=1 spin state. The temperature dependence of the spin-lattice relaxation of the radical pair of flavin and tyrosine in SyPixD was investigated by pulsed electron paramagnetic resonance(EPR) spectroscopy. Based on the temperature dependence of spin. lattice relaxation time T-1, the exchange coupling of the radical pair was estimated as 2J=6 to 8 cm^<-1>, defined as-2JS-1* S-2.
The radical pair was utilized as a probe to analyze the oligomer of SyPixD. The relative arrangement of PixD proteins in the complex was investigated by pulsed electron-electron double resonance(PELDOR) with the orientation selection. Based on the decameric structure in the crystal, the possible structure for the PELDOR results was evaluated.
The transient EPR measurements have been performed to investigate the reaction pathway to produce radical-pair. The results show that the radical-pair was produced in the range of 200-263 K. The possible reaction mechanism was proposed.
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Report
(4 results)
Research Products
(21 results)
