Regulation of genome integrity through a DNA replication coupled feedback control
| Project/Area Number |
21370081
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | University of Hyogo |
|---|
Principal Investigator |
NISHITANI Hideo 兵庫県立大学, 大学院・生命理学研究科, 教授 (40253455)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIOMI Yasushi 兵庫県立大学, 大学院・生命理学研究科, 助教 (80380567)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
|---|
| Keywords | DNA / 複製 / 細胞周期 / タンパク質分解 / DNA損傷 |
|---|
| Research Abstract |
To ensure that DNA replication is limited to only once during a cell cycle, initiator protein Cdt1 is degraded once DNA replication is initiated. We examined how PCNA dependent CRL4-Cdt2 operates for the degradation of Cdt1.We report that CRL4-Cdt2 recognizes specific residues located at the N-terminus of Cdt1 and that PCNA loaders are differentially required during S phase and repair. Cdt1 degradation is strictly regulated to maintain genome integrity.
|
Report
(4 results)
Research Products
(42 results)
