| Budget Amount *help |
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Research Abstract |
Precise regulation of DNA replication reaction is essential for integrity of genome information. Cdt1, together with ORC and CDC6, loads MCM replication helicase complex onto DNA replication origin. GRWD1 has been identified as a novel Cdt1-binding protein. In this research project, we have obtained following findings.(GRWD1 binds to Cdc6 and Cdt1, thereby accumulates onto replication origins.(2) GRWD1is a novel histone-binding protein and has a histone chaperon activity. Furthermore, GRWD1is actually involvedin regulation of chromatin openness(in general, it is believed that open chromatin structure promotes various transactions on chromatin).(3) GRWD1 silencing by siRNA inhibits MCM loading. Therefore, GRWD1may play a crucial role in regulation ofreplication.
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