Analysis of GRWD1, a novel histone-binding protein involved in chromosomal replication initiation and segregation
| Project/Area Number |
21370084
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University (2010-2011)
National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East (2009) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
WAGA Shou 日本女子大学, 理学部, 教授 (60222402)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | GRWD1 / Cdt1 / 複製開始複合体 / 細胞周期 / ヒストンシャペロ / MCM / クロマチン / 次世代シークエンシング / 複製開始制御 / ヒストンシャペロン / ライセンシング制御 / 複製開始点 |
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| Research Abstract |
Precise regulation of DNA replication reaction is essential for integrity of genome information. Cdt1, together with ORC and CDC6, loads MCM replication helicase complex onto DNA replication origin. GRWD1 has been identified as a novel Cdt1-binding protein. In this research project, we have obtained following findings.(GRWD1 binds to Cdc6 and Cdt1, thereby accumulates onto replication origins.(2) GRWD1is a novel histone-binding protein and has a histone chaperon activity. Furthermore, GRWD1is actually involvedin regulation of chromatin openness(in general, it is believed that open chromatin structure promotes various transactions on chromatin).(3) GRWD1 silencing by siRNA inhibits MCM loading. Therefore, GRWD1may play a crucial role in regulation ofreplication.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] CDC6interaction with ATR regulates activation ofa replication checkpoint in higher eukaryotic cells2010
Author(s)
Yoshida, K., Sugimoto, N., Iwahori, S., Yugawa, T., Narisawa-Saito, M., Kiyono, T. & Fujita
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Journal Title
J. Cell Sci
Volume: 123
Pages: 225-235
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[Journal Article] Redundant and differential regulation of multiple licensing factors ensures prevention of rereplication in normal human cells2009
Author(s)
Sugimoto, N., Yoshida, K., Tatsumi, Y., Yugawa, T., Narisawa-Saito, M., Waga, S., Kiyono, T. & Fujita, M.
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Journal Title
J. Cell Sci
Volume: 122
Pages: 1184-1191
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[Presentation] The Cdt1-binding protein GRWD1 is a novel histone chaperone involved in replication licensing.2011
Author(s)
Sugimoto, N., Yasukouchi, S., Watanabe, S., Kiyono, T., Kurumizaka, H. & Fujita, M.
Organizer
第34回日本分子生物学会年会ワークショップ
Place of Presentation
パシフィコ横浜
Year and Date
2011-12-16
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