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Analysis of GRWD1, a novel histone-binding protein involved in chromosomal replication initiation and segregation

Research Project

Project/Area Number 21370084
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionKyushu University (2010-2011)
National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East (2009)

Principal Investigator

FUJITA Masatoshi  九州大学, 薬学研究院, 教授 (30270713)

Co-Investigator(Kenkyū-buntansha) WAGA Shou  日本女子大学, 理学部, 教授 (60222402)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsGRWD1 / Cdt1 / 複製開始複合体 / 細胞周期 / ヒストンシャペロ / MCM / クロマチン / 次世代シークエンシング / 複製開始制御 / ヒストンシャペロン / ライセンシング制御 / 複製開始点
Research Abstract

Precise regulation of DNA replication reaction is essential for integrity of genome information. Cdt1, together with ORC and CDC6, loads MCM replication helicase complex onto DNA replication origin. GRWD1 has been identified as a novel Cdt1-binding protein. In this research project, we have obtained following findings.(GRWD1 binds to Cdc6 and Cdt1, thereby accumulates onto replication origins.(2) GRWD1is a novel histone-binding protein and has a histone chaperon activity. Furthermore, GRWD1is actually involvedin regulation of chromatin openness(in general, it is believed that open chromatin structure promotes various transactions on chromatin).(3) GRWD1 silencing by siRNA inhibits MCM loading. Therefore, GRWD1may play a crucial role in regulation ofreplication.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (26 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 7 results) Presentation (11 results) Remarks (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome2012

    • Author(s)
      Egawa, N., et al
    • Journal Title

      J.Virol.

      Volume: 86 Issue: 6 Pages: 3276-3281

    • DOI

      10.1128/jvi.06450-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chromatin remodeler Sucrose Non-Fermenting 2Homolog(SNF2H) is recruited onto DNAreplication origins through interaction with Cdc10 protein-dependent transcript 1(Cdt1) and promotes pre-replication complex formation2011

    • Author(s)
      Sugimoto, N., Yugawa, T., Iizuka, M., Kiyono, T. & Fujita, M.
    • Journal Title

      J. Biol. Chem

      Volume: 286 Pages: 39200-39210

    • Related Report
      2011 Final Research Report
  • [Journal Article] Chromatin remodeler sucrose nonfermenting 2 homolog (SNF2H) is recruited onto DNA replication origins through interaction with Cdc10 protein-dependent transcript 1 (Cdt1) and promotes pre-replication complex formation2011

    • Author(s)
      Nozomi Sugimoto
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286 Issue: 45 Pages: 39200

    • DOI

      10.1074/jbc.m111.256123

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An in vitro multistep carcinogenesis model for both HPV-positive and -negative human oral squamous cell carcinoma2011

    • Author(s)
      Zushi, Y., et al
    • Journal Title

      Am.J.Cancer Res.

      Volume: 1 Pages: 869-881

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CDC6interaction with ATR regulates activation ofa replication checkpoint in higher eukaryotic cells2010

    • Author(s)
      Yoshida, K., Sugimoto, N., Iwahori, S., Yugawa, T., Narisawa-Saito, M., Kiyono, T. & Fujita
    • Journal Title

      J. Cell Sci

      Volume: 123 Pages: 225-235

    • Related Report
      2011 Final Research Report
  • [Journal Article] CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells2010

    • Author(s)
      Kazumasa Yoshida
    • Journal Title

      J.Cell Sci

      Volume: 123 Pages: 225-235

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells2010

    • Author(s)
      Kazumasa Yoshida
    • Journal Title

      J.Cell Sci 123

      Pages: 225-235

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redundant and differential regulation of multiple licensing factors ensures prevention of rereplication in normal human cells2009

    • Author(s)
      Sugimoto, N., Yoshida, K., Tatsumi, Y., Yugawa, T., Narisawa-Saito, M., Waga, S., Kiyono, T. & Fujita, M.
    • Journal Title

      J. Cell Sci

      Volume: 122 Pages: 1184-1191

    • Related Report
      2011 Final Research Report
  • [Journal Article] Redundant and differential regulation of multiple licensing factors ensures prevention of rereplication in normal human cells2009

    • Author(s)
      Nozomi Sugimoto
    • Journal Title

      J.Cell Sci 122

      Pages: 1184-1191

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histone acetyltransferase Hbo1 : catalytic activity, cellular abundance, and links to primary cancers2009

    • Author(s)
      Masayoshi Iizuka
    • Journal Title

      Gene 436

      Pages: 108-114

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] The Cdt1-binding protein GRWD1 is a novel histone chaperone involved in replication licensing.2011

    • Author(s)
      Sugimoto, N., Yasukouchi, S., Watanabe, S., Kiyono, T., Kurumizaka, H. & Fujita, M.
    • Organizer
      第34回日本分子生物学会年会ワークショップ
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Final Research Report
  • [Presentation] The Cdt1-binding protein GRWD1 is a novel histone chaperone involved in replication licensing2011

    • Author(s)
      Nozomi Sugimoto, et al
    • Organizer
      第34回日本分子生物学会年会ワークショップ
    • Place of Presentation
      パシフィコ横浜(招待講演)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] A novel function of ATM during the unperturbed S phase : Regulation of Cdt1 proteolysis and prevention of rereplication2011

    • Author(s)
      Satoko Iwahori, et al
    • Organizer
      Cold Spring Harbor Laboratory 2011 Meeting on Eukaryotic DNA Replication & Genome Maintenance
    • Place of Presentation
      Cold Spring Harbor, NY, USA
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] A novel function of ATM : regulation of Cdt1 during the S phase2010

    • Author(s)
      Masatoshi Fujita
    • Organizer
      Biochemistry and Molecular Biology 2010, Symposium
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] A chromatin remodeler SNF2H promotes pre-RC formation via Cdt1 interaction2010

    • Author(s)
      Nozomi Sugimoto
    • Organizer
      Biochemistry and Molecular Biology 2010
    • Place of Presentation
      神戸ポートアイランド(一般口頭発表)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 複製開始複合体形成に関与しているCdt1結合性ヒストン制御蛋白質2010

    • Author(s)
      藤田雅俊
    • Organizer
      大阪大学蛋白質研究所セミナー「ゲノム機能の記憶の成立機序とその制御」
    • Place of Presentation
      大阪大学蛋白質研究所
    • Year and Date
      2010-11-19
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells2009

    • Author(s)
      Kazumasa Yoshida
    • Organizer
      Keystone Symposia "Telomere Biology and DNA Repair"
    • Place of Presentation
      Ashmore, Queensland, Australia
    • Year and Date
      2009-10-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] A chromatin remodeler SNF2H is involved in pre-RC formation and Cdt1-induced checkpoint activation2009

    • Author(s)
      Nozomi Sugimoto
    • Organizer
      Keystone Symposia "Telomere Biology and DNA Repair"
    • Place of Presentation
      Ashmore, Queensland, Australia
    • Year and Date
      2009-10-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 正確にゲノムを倍加させる:複製開始因子Cdt1の分解制御機構の解明と抗がん剤開発への応用2009

    • Author(s)
      藤田雅俊
    • Organizer
      福井大学大学院工学研究科、招請研究セミナー
    • Place of Presentation
      福井
    • Year and Date
      2009-05-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] A chromatin remodeler SNF2H is involved in pre-RC formation and Cdt1-induced checkpoint activation2009

    • Author(s)
      Sugimoto, N., Waga, S., Kiyono, T. & Fujita, M.
    • Organizer
      Keystone Symposia "Telomere Biology and DNA Repair"
    • Place of Presentation
      Ashmore, Queensland, Australia
    • Related Report
      2011 Final Research Report
  • [Presentation] CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells2009

    • Author(s)
      Yoshida, K., Iwahori, S., Kiyono, T. & Fujita, M.
    • Organizer
      Keystone Symposia"Telomere Biology and DNA Repair"
    • Place of Presentation
      Ashmore, Queensland, Australia
    • Related Report
      2011 Final Research Report
  • [Remarks] ホームページ:

    • URL

      http://tansaku.phar.kyushu-u.ac.jp/saito/top.html

    • Related Report
      2011 Final Research Report
  • [Remarks] 九州大学薬学研究院公開講座:遺伝情報を過不足無く受け継ぐためのしくみ?分子生物学的解明と医薬研究への応用?」平成22年9月26日、九州大学

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://tansaku.phar.kyushu-u.ac.jp/saito/top.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://tansaku.phar.kyushu-u.ac.jp/saito/top.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] G2/M期停止及び細胞死を誘導するベンゾヒドラジド誘導体2011

    • Inventor(s)
      藤田雅俊、大嶋孝志、森本浩之
    • Industrial Property Rights Holder
      九州大学
    • Industrial Property Number
      2011-234309
    • Filing Date
      2011-10-25
    • Related Report
      2011 Annual Research Report 2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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