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Studies on cell functions regulated by changes in membrane phospholipid asymmetry

Research Project

Project/Area Number 21370085
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionHokkaido University

Principal Investigator

TANAKA Kazuma  北海道大学, 遺伝子病制御研究所, 教授 (60188290)

Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Keywords生体膜 / 脂質の非対称性 / フリッパーゼ / 細胞内小胞輸送 / 酵母 / 膜脂質の非対称性 / フリッペース / フォスファチジルセリン / エンドソーム / ゴルジ体 / クラスリンアダプター / クラスリン / AP-1 / 細胞極性
Research Abstract

Lipids are essential components of cell membranes. Abnormal distribution or transport of lipids is related to diseases, but much remains to be learned. In this study, we focus on phospholipid flippases, which transport phospholipids to the cytoplasmic leaflet of membranes, and have shown that flippases are involved in protein sorting and vesicle formation. We have also identified new genes that regulate phospholipid asymmetry of intracellular membranes.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (41 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (29 results)

  • [Journal Article] Identification of a second amphipathic lipid-packing sensor like motif that contributes to Gcs1p function in the early endosome-to-TGN pathway2013

    • Author(s)
      Zahra Zendeh-boodi, Takaharu Yamamoto, Hiroshi Sakane, and Kazuma Tanaka
    • Journal Title

      J Biochem

      Volume: 153 Issue: 6 Pages: 573-587

    • DOI

      10.1093/jb/mvt025

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast2013

    • Author(s)
      Hachiro T, Yamamoto T, Nakano K, Tanaka K
    • Journal Title

      J Biol Chem

      Volume: 288 Issue: 5 Pages: 3594-3608

    • DOI

      10.1074/jbc.m112.416263

    • NAID

      120005228251

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a second amphipathic lipid-packing sensor like motif that contributes to Gcs1p function in the early endosome-to-TGN pathway.2013

    • Author(s)
      Zahra Zendeh-boodi,Takaharu Yamamoto, Hiroshi Sakane, and Kazuma Tanaka.
    • Journal Title

      J Biochem

      Volume: in press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutations in N-terminal Flanking Region of Blue Light-sensing Light-Oxygen and Voltage 2 (LOV2) Domain Disrupt Its Repressive Activity on Kinase Domain in the Chlamydomonas Phototropin2012

    • Author(s)
      Yusuke Aihara, Takaharu Yamamoto, Koji Okajima, Kazuhiko Yamamoto, Tomomi Suzuki, Satoru Tokutomi, Kazuma Tanaka, Akira Nagatani
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Issue: 13 Pages: 9901-9909

    • DOI

      10.1074/jbc.m111.324723

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Essential Role of the NH2-Terminal Region of Cdc24 Guanine Nucleotide Exchange Factor in Its Initial Polarized Localization in Saccharomyces cerevisiae2012

    • Author(s)
      Fujimura-Kamada K, Hirai T, Tanaka K
    • Journal Title

      Eukaryot Cell

      Volume: 11 Issue: 1 Pages: 2-15

    • DOI

      10.1128/ec.05146-11

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of novel mutations in CDC50,the non-catalytic subunit of the Drs2p phospholipid flippase2012

    • Author(s)
      Takahashi Y, Fujimura-Kamada K, Kondo S, and Tanaka K
    • Journal Title

      J Biochem

      Volume: 149 Issue: 4 Pages: 423-432

    • DOI

      10.1093/jb/mvq155

    • NAID

      10030584677

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Konomi Fujimura-Kamada and Takaharu YamamotoFunctions of phospholipid flippases2011

    • Author(s)
      Kazuma Tanaka
    • Journal Title

      J Biochem

      Volume: 149 Issue: 2 Pages: 131-143

    • DOI

      10.1093/jb/mvq140

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 出芽酵母の細胞極性形成における エンドサイトーシス-リサイクリング経路の役割アクチンケーブルが形成されない変異株からわかったこと2011

    • Author(s)
      山本隆晴,田中一馬
    • Journal Title

      化学と生物

      Volume: 49 Pages: 231-233

    • NAID

      10028117192

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functions of phospholipid flippases.2011

    • Author(s)
      Tanaka K, Fujimura-Kamada K, Yamamoto T.
    • Journal Title

      J Biochem.

      Volume: 149 Pages: 131-143

    • NAID

      10030583216

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of novel mutations in CDC50, the non-catalytic subunit of the Drs2p phospholipid flippase.2011

    • Author(s)
      Takahashi Y, Fujimura-Kamada K, Kondo S, Tanaka K.
    • Journal Title

      J Biochem.

      Volume: 149 Pages: 423-432

    • NAID

      10030584677

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Initial polarized bud growth byendocytic recycling in the absence of actin cable-dependent vesicle transport in yeast2010

    • Author(s)
      Yamamoto T, Mochida J, Kadota J, Takeda M, Bi E, and Tanaka K
    • Journal Title

      Mol Biol Cell

      Volume: 21 Issue: 7 Pages: 1237-1252

    • DOI

      10.1091/mbc.e09-05-0412

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Initial Polarized Bud Growth by Endocytic Recycling in the Absence of Actin Cable-depen dent Vesicle Transpot in Yeast

    • Author(s)
      Yamamoto T., et al
    • Journal Title

      Molecular Biology of the Cell (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] エンドサイトーシスーリサイクリング経路におけるアレスチン様タンパク質Art5pとDnf1/2pリン脂質フリッパーゼとの相互作用2012

    • Author(s)
      山神香菜子,山本隆晴,武田美代子,湯田彬子,田中一馬
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Isolation and characterization of novel mutations in CDC50, thenon-catalyticsubunit ofthe Drs2p phospholipid flippase.2012

    • Author(s)
      Konomi Fujimura-Kamada, Yasuhiro Takahashi, Satoshi Kondo, and Kazuma Tanaka
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Isolation and characterization of novel mutations in CDC50, the non-catalytic subunit of the Drs2p phospholipid flippase.2012

    • Author(s)
      Konomi Fujimura-Kamada, Yasuhiro Takahashi, Satoshi Kondo, and Kazuma Tanaka.
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      マリンメッセ福岡(福岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] エンドサイトーシスーリサイクリング経路におけるアレスチン様タンパク質Art5pとDnf1/2pリン脂質フリッパーゼとの相互作用2012

    • Author(s)
      山神 香菜子、山本 隆晴、武田 美代子、湯田 彬子、田中 一馬
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      マリンメッセ福岡(福岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細胞内小胞輸送におけるリン脂質フリッパーゼの役割2011

    • Author(s)
      山本隆晴,鎌田このみ,花松久寿,ZahraZendehboodi,武田美代子,田中一馬
    • Organizer
      第48回日本生化学会北海道支部例会
    • Place of Presentation
      札幌医科大学記念ホール
    • Year and Date
      2011-08-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内小胞輸送におけるリン脂質フリッパーゼの役割2011

    • Author(s)
      山本隆晴、鎌田このみ、花松久寿、Zahra Zendehboodi、武田美代子、田中一馬
    • Organizer
      第48回日本生化学会北海道支部例会
    • Place of Presentation
      札幌医科大学(札幌市)
    • Year and Date
      2011-08-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 膜輸送におけるリン脂質フリッパーゼの役割2011

    • Author(s)
      山本隆晴,鎌田このみ,田中一馬
    • Organizer
      第63回細胞生物学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-06-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 膜輸送におけるリン脂質フリッパーゼの役割Roles of phospholipid flippases in membrane trafficking2011

    • Author(s)
      山本隆晴, 鎌田このみ, 田中一馬
    • Organizer
      第63回細胞生物学会大会
    • Place of Presentation
      北海道大学(札幌市)(招待講演)
    • Year and Date
      2011-06-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] The Role of Phospholipid Asymmetry in Vesicle Transport in the Early Endosome-to-TGN Retrieval Pathway.2011

    • Author(s)
      武田美代子,山本隆晴,田中一馬
    • Organizer
      第63回細胞生物学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-06-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Functions of the non-catalytic region of Gcs1p in the phospholipid flippase- regulatingmembranetrafficking pathway2011

    • Author(s)
      Zahra Zendehboodi,Takaharu Yamamoto,Kazuma Tanaka
    • Organizer
      第63回細胞生物学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-06-27
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of phospholipid asymmetry in the localization of tryptophan permease Tat2p.2011

    • Author(s)
      鉢呂健,山本隆晴,田中一馬
    • Organizer
      第63回細胞生物学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-06-27
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of phospholipid asymmetry in the localization of tryptophan permease Tat2p2011

    • Author(s)
      鉢呂健, 山本隆晴, 田中一馬
    • Organizer
      第63回細胞生物学会大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functions of the non-catalytic region of Gcs1p in the phospholipid flippase-regulatingmembrane trafficking pathway2011

    • Author(s)
      Zahra Zendehboodi, Takaharu Yamamoto, Kazuma Tanaka
    • Organizer
      第63回細胞生物学会大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] The Role of Phospholipid Asymmetry in Vesicle Transport in the Early Endosome-to-TGN Retrieval Pathway2011

    • Author(s)
      武田美代子, 山本隆晴, 田中一馬
    • Organizer
      第63回細胞生物学会大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞膜ホスファチジルセリン非対称性はどのように形成されるのか?2010

    • Author(s)
      三岡哲生,鎌田このみ,田中一馬
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-09
    • Related Report
      2012 Final Research Report
  • [Presentation] How is the plasma membrane phosphatidylserine (PS) asymmetry generated in Saccharomyces cerevisiae?2010

    • Author(s)
      三岡哲生、鎌田このみ、田中一馬
    • Organizer
      BMB2010 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Yeast phospholipid translocase Drs2- Cdc50 functions with an F-boxprotein Rcy1 in endocytic recycling.2010

    • Author(s)
      花松久寿,鎌田このみ,田中一馬
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Yeast phospholipid translocase Drs2-Cdc50 functions with an F-box protein Rcy1 in endocytic recycling2010

    • Author(s)
      花松久寿、鎌田このみ、田中一馬
    • Organizer
      BMB2010 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Roles of phospholipid flippases and their related ArfGAP for protein trafficking.2010

    • Author(s)
      Takaharu Yamamoto, Takeru Hachiro, Zahra Zendehboodi, Kazuma Tanaka
    • Organizer
      The 27th NAITO CONFERENCE on MembraneDynamics and Lipid Biology [I]
    • Place of Presentation
      シャトレーゼガトーキングダム札幌
    • Year and Date
      2010-07-02
    • Related Report
      2012 Final Research Report
  • [Presentation] The functional analysis of phospholipid flippase in endocytic recycling.2010

    • Author(s)
      Konomi Fujimura-Kamada, Hisatoshi Hanamatsu, Koji Saito, Eno Shioji, Rosa Suzuki, Kazuma Tanaka
    • Organizer
      The 27th NAITO CONFERENCE on MembraneDynamics and Lipid Biology [I]
    • Place of Presentation
      シャトレーゼガトーキングダム札幌
    • Year and Date
      2010-07-02
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulation and functional significance of phospholipid asymmetry.2010

    • Author(s)
      Kazuma Tanaka
    • Organizer
      The 27th NAITO CONFERENCE on MembraneDynamics and Lipid Biology [I].
    • Place of Presentation
      シャトレーゼガトーキングダム札幌
    • Year and Date
      2010-07-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulation and functional significance of phospholipid asymmetry2010

    • Author(s)
      田中一馬、鎌田このみ、山本隆晴
    • Organizer
      第27回内藤コンファレンス
    • Place of Presentation
      シャトレーゼ ガトーキングダムサッポロ(札幌市) 招待講演
    • Year and Date
      2010-07-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Roles of phospholipid flippases and their related ArfGAP for protein trafficking.2010

    • Author(s)
      Takaharu Yamamoto, Takeru Hachiro, Zahra Zendehboodi, Kazuma Tanaka
    • Organizer
      Sapporo International Cancer Symposium2010.
    • Place of Presentation
      北海道大学
    • Year and Date
      2010-06-28
    • Related Report
      2012 Final Research Report
  • [Presentation] The functional analysis of phospholipid flippase in endocytic recycling.2010

    • Author(s)
      Konomi Fujimura-Kamada, Hisatoshi Hanamatsu, Koji Saito, Eno Shioji, Rosa Suzuki, Kazuma Tanaka
    • Organizer
      Sapporo International Cancer Symposium2010.
    • Place of Presentation
      北海道大学
    • Year and Date
      2010-06-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 膜脂質非対称性の制御機構と細胞機能2010

    • Author(s)
      田中一馬
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] フリッペースが制御する膜脂質非対称性とその細胞機能2009

    • Author(s)
      田中一馬
    • Organizer
      第82回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートピアホテル
    • Year and Date
      2009-10-24
    • Related Report
      2012 Final Research Report
  • [Presentation] フリッペースが制御する膜脂質非対称性とその細胞機能2009

    • Author(s)
      田中一馬
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cellular functions of phospholipid Flippases.2009

    • Author(s)
      Kazuma Tanaka
    • Organizer
      The fourth iCeMS International Symposium
    • Place of Presentation
      ホテルフジタ京都
    • Year and Date
      2009-05-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Cellular functions of phospholipid flippases2009

    • Author(s)
      田中一馬
    • Organizer
      The fourth iCeMS International Symposium
    • Place of Presentation
      ホテルフジタ京都(京都市)
    • Year and Date
      2009-05-28
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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