Assembly mechanisms of the centriole precursor
| Project/Area Number |
21370088
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIRONO Masafumi 東京大学, 大学院・理学系研究科, 准教授 (10212177)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 細胞骨格・運動 / 微小管 / 細胞骨格 / クラミドモナス / 基底小体 |
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| Research Abstract |
The centriole's nine-fold symmetry is largely determined by its two precursor structures, the amorphous ring and cartwheel. However, assembly mechanisms of these structures are totally unknown. In this study, we identified a Chlamydomonas protein CRC70 as a strong candidate for an amorphous ring component, and showed that this protein induces accumulation of centriolar component when overexpressed in Chlamydomonas and mouse cells. Furthermore, by functional analysis of SAS-6 based on its X-ray crystal structure, we showed that the central part of the cartwheel is composed of radially arranged nine SAS-6 dimers.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Structures of SAS-6 suggest its organization in centrioles2011
Author(s)
van Breugel,M.,Hirono,M.,Andreeva,A.,Yanagisawa,H. A.,Yamaguchi,S.,Nakazawa,Y.,Morgner,N.,Petrovich,M.,Ebong,I.O.,Robinson,C.V.,Johnson,C.M.,Veprintsev,D.,Zuber,B.
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Journal Title
Science
Volume: 331
Issue: 6021
Pages: 1196-1199
DOI
Related Report
Peer Reviewed
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[Presentation] Two novel coiled-coil axonemal proteins, Mia1 and Mia2, are required for phototaxis and normal flagellar motility in Chlamydomonas reinhardtii2010
Author(s)
Yamamoto,R.,Yanagaisawa,H.,Yagi,T.,Hirono,M.,Kamiya,R.
Organizer
ASCB Annual Meeting
Place of Presentation
Philadelphia, Pennsylvania, USA
Related Report
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[Presentation] The Chlamydomonas flagellar I1 dynein assembles a 20S complex in the wild-type and ida3 cytoplasm, but the I1 complex fails to enter the flagellum in the ida3 mutant2010
Author(s)
Viswanadha,R.,Wirschell,M.,Fox,L.,Kramer,T.,Kamiya,R.,Hirono,M.,Sale,W.S.
Organizer
ASCB Annual Meeting
Place of Presentation
Philadelphia, Pennsylvania, USA
Related Report
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[Presentation] A temperature- sensitive mutant in retrograde intraflagellar transport (IFT) reveals that retrograde IFT carries a distinct set of cargo proteins and is required for the maintenance of flagellar function but not flagellar length.2010
Author(s)
Engel,B.D.,Wakabayashi, K.,Engel,K.B.,Geimer,S.,Hirono,M.,Kamiya,R.,and Marshall,W.F.
Organizer
ASCB Annual Meeting
Place of Presentation
Philadelphia, Pennsylvania, USA
Related Report
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[Presentation] Possible involvement of dynein regulatory complex in the regulation of inner arm dynein function by tubulin polyglutamylation2010
Author(s)
Kubo,T.,Yanagisawa,H.,Yagi,T.,Hirono,M.,Kamiya,R.
Organizer
FASEB Summer Research ConferencesBiology of Cilia and Flagella
Place of Presentation
Saxtons River, Vermont, USA
Related Report
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[Presentation] A novel Chlamydomonasinner-arm-dynein-deficient mutant, ida10, harbors a mutation in a conserved protein with a PIH domain2010
Author(s)
Yamamoto,R.,Hirono,M.,Kamiya,R.
Organizer
14th International Conference on the Cell and Molecular Biology of Chlamydomonas
Place of Presentation
Wheaton College, Norton, MA, USA
Related Report
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