| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Research Abstract |
The centriole's nine-fold symmetry is largely determined by its two precursor structures, the amorphous ring and cartwheel. However, assembly mechanisms of these structures are totally unknown. In this study, we identified a Chlamydomonas protein CRC70 as a strong candidate for an amorphous ring component, and showed that this protein induces accumulation of centriolar component when overexpressed in Chlamydomonas and mouse cells. Furthermore, by functional analysis of SAS-6 based on its X-ray crystal structure, we showed that the central part of the cartwheel is composed of radially arranged nine SAS-6 dimers.
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