Molecular and functional analysis of Notch signaling in organogenesis

• Project/Area Number: 21370090
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Chiba University (2011); Nagoya University (2009-2010)
• Principal Investigator: MOTOYUKI Itoh 千葉大学, 大学院・薬学研究院, 教授 (20377906)
• Co-Investigator(Renkei-kenkyūsha): KOICHI Kawakami 国立遺伝学研究所, 個体遺伝研究系, 教授 (70195048)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 器官形成 / Notch zebrafish / zebrafish / notch / jagged / ゼブラフィッシュ / Notch / 脊索 / 神経 / Notch signal / notochord / neuron

Research Abstract

Notch signaling is an evolutionary conserved pathway that plays an important role in the development of various organs. In this study, physiological functions of Notch ligands and the mechanism by which Notch activity is regulated were analyzed using zebrafish. We found a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signaling. Furthermore, using a biochemical screen, we identified Notch as a new substrate of NLK. NLK-phosphorylated Notch1 intercellular domain is impaired in its ability to form a transcriptionally active ternary complex.

Research Products

• [Journal Article] Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions (2010)
  • Author(s): Yamamoto, M., Morita, R., Mizoguchi, T., Matsuo, H., Isoda, M., Ishitani, T., Chitnis, A. B., Matsumoto, K., Crump, J. G., Hozumi, K., Yonemura, S., Kawakami, K., and Itoh, M
  • Journal Title: Development Volume: 137 Issue: 15 Pages: 2527-2537
  • DOI: 10.1242/dev.051011
  • Peer Reviewed
• [Journal Article] Delta1 family members are involved in filopodial actin formation and neuronal cell migration independent of Notch signaling (2010)
  • Author(s): Sugiyama K, Nishide K, Matsuo H, Okigawa S, Okano M, Ishitani T, Matsumoto K, Itoh M.
  • Journal Title: Biochem Biophys Res Commun Volume: 398 Issue: 1 Pages: 118-124
  • DOI: 10.1016/j.bbrc.2010.06.047
  • Peer Reviewed
• [Journal Article] Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex (2010)
  • Author(s): Ishitani, T., Hirao, T., Suzuki, M., Isoda, M., Ishitani, S., Harigaya, K., Kitagawa, M., Matsumoto, K., and Itoh, M
  • Journal Title: Nature Cell Biol Volume: 12 Issue: 3 Pages: 278-285
  • DOI: 10.1038/ncb2028
  • NAID: 120005293254
  • Peer Reviewed
• [Journal Article] Nemo-like kinase suppresses Notch signalling by interfering with for mation of the Notch active transcriptional complex (2010)
  • Author(s): Ishitani T, Hirao T, Suzuki M, Isoda M, Ishitani S, Harigaya K, Kitagawa M, Matsumoto K, Itoh M
  • Journal Title: Nat.Cell Biol. 12 Pages: 278-295
  • Peer Reviewed
• [Journal Article] Nemo-like kinase is involved in NGF-induced neurite outgrowth via phosphorylating MAP1B and paxillin (2009)
  • Author(s): Ishitani, T., Ishitani, S., Matsumoto, K., and Itoh, M
  • Journal Title: J. Neurochem Volume: 111 Issue: 5 Pages: 1104-1118
  • DOI: 10.1111/j.1471-4159.2009.06400.x
  • Peer Reviewed
• [Journal Article] Nemo-like kinase is involved in NGF-induced neurite outgrowth via phosphorylating MAP1B and paxillin (2009)
  • Author(s): Ishitani T, Ishitani S, Matsumoto K, Itoh M.
  • Journal Title: J.Neurochem. 111 Pages: 1104-1118
  • Peer Reviewed
• [Presentation] Mib1 is involved in cell migration via Notch independent pathway. (2012)
  • Author(s): Takamasa Mizoguchi, Kazuya Hirose, Motoyuki Itoh
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] Mind bomb1 is required for trans-endocytosis of Notchinto ligand-expressing cells to activate Notch signaling (2011)
  • Author(s): Makoto Okano, Hiromi Matsuo, Katsuto Hozumi, Motoyuki Itoh
  • Organizer: 第44回大会 日本発生生物 学会
  • Place of Presentation: 沖縄コンベンション センター
  • Year and Date: 2011-05-20
• [Presentation] Mind bomb1 is required for trans-endocytosis of Notch into ligand-expressing cells to activate Notch signaling (2011)
  • Author(s): Makoto Okano, Hiromi Matsuo, Katsuto Hozumi, Motoyuki Itoh
  • Organizer: 第44回大会日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-20
• [Presentation] DeltaA and DeltaD act cooperatively to maintain V2 interneuron progenitors (2010)
  • Author(s): 沖川沙佑美,磯田美 帆, Maximiliano Suster , 川上浩一 , 伊 藤 素 行
  • Organizer: 第33回日本分子生 物学会年会
  • Place of Presentation: 神戸 ポート アイランド
  • Year and Date: 2010-12-07
• [Presentation] 小型魚類モデルでの分子機能解析に有効なアンチセンス核酸 (2010)
  • Author(s): 伊藤素行
  • Organizer: 第20回アンチセンスシンポジウム
  • Place of Presentation: 甲南大学ポートアイランドキャンパス
  • Year and Date: 2010-12-03
• [Presentation] Mind bomb1 is required for transendocytosis of Notch in signal-sending cells to activate Notch signaling (2010)
  • Author(s): 岡野誠、松尾宏美、穂積勝人、 伊 藤 素 行
  • Organizer: 第5回 Notch 研究会
  • Place of Presentation: 東京理科大 野田キャンパス
• [Presentation] Mind bomb1 is required for transendocytosis of Notch in signal-sending cells to activate Notch signaling (2010)
  • Author(s): 岡野誠、松尾宏美、穂積勝人、伊藤素行
  • Organizer: 第5回Notch研究会
  • Place of Presentation: 東京理科大野田キャンパス
• [Presentation] Maintenance of neuronal progenitors requires the DeltaA in zebrafish (2009)
  • Author(s): 沖 川 沙 佑 美 、 磯 田 美 帆、Maximiliano Suster、川上浩一、 伊 藤素 行
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシィフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Jagged-NotchシグナルはNotochord細胞の分化・運命決定を通じて、周囲組織のパターン形成活性と体軸支持構造形成を調節する (2009)
  • Author(s): 盛田良子、山本麻衣、溝口貴正、磯田美帆、松本邦弘、米村重信、川上浩一、伊藤素行
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシィフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Jagged-Notch シグナルは Notochord 細胞の分化・運命決定を通じて、周囲組織のパターン形成活性と体軸支持構造形成を調節する (2009)
  • Author(s): 盛田良子, 山本麻衣, 溝口貴正, 磯由美帆, 松本邦弘, 米村重信, 川上浩一, 伊藤素行
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシィフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Jagged-Notch signaling regulates cell fate decisions during notochord development in zebrafish 4th Asia-Oceania zebrafish meeting (2009)
  • Author(s): 伊 藤素行
  • Place of Presentation: Jeju, Korea
• [Presentation] Jagged-Notch signaling regulates cell fate decisions during notochord development in zebrafish (2009)
  • Author(s): 伊藤素行
  • Organizer: 4th Asia-Oceania zebrafish meeting
  • Place of Presentation: Jeju, Korea
• [Remarks]
  • URL: http://www.p.chiba-.ac.jp/lab/seika/index.html
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/index2.html
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/index2.html