| Project/Area Number |
21370093
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
OKANO Masaki 独立行政法人理化学研究所, 哺乳類エピジェネティクス研究チーム, チームリーダー (50360863)
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| Co-Investigator(Renkei-kenkyūsha) |
TAMURA Takashi 独立行政法人理化学研究所, 哺乳類エピジェネティクス研究チーム, 研究員 (20372659)
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| Research Collaborator |
MATSUOKA Chisa 独立行政法人理化学研究所, 哺乳類エピジェネティクス研究チーム, テクニカルスタッフ
YAMAGIWA Akiko 独立行政法人理化学研究所, 哺乳類エピジェネティクス研究チーム, テクニカルスタッフ
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | エピジェネティクス / DNAメチル化 / Dnmt1 / DNA複製 / 細胞核 / 維持メチル化 |
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| Research Abstract |
DNA methyltransferase 1(Dnmt1) plays an important role in the inheritance of genomic DNA methylation in mammals. Dnmt1 localizes to DNA replication foci in a cell-cycle-dependent manner. However, it remains elusive which domains of Dnmt1 are required for this process and how this process is regulated. By scanning mutagenesis, we identified several domains required for the regulation of Dnmt1 localization. Our results suggest that the intra-molecular interaction is important for Dnmt1 localization.
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