Development of molecular specific nano-imaging technology based on live CLEM
Project/Area Number |
21370094
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Cell biology
|
Research Institution | National Institute of Information and Communications Technology |
Principal Investigator |
HARAGUCHI Tokuko 独立行政法人情報通信研究機構, 未来ICT研究所, 上席研究員 (20359079)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | 細胞構造・機能 / 蛋白質 / 生体分子 / バイオイメージング / 細胞・組織 / 遺伝子 |
Research Abstract |
This research aims to develop an imaging technology, so called "Live CLEM", applicable to non-adherent cells including yeasts and ciliates. In this study, we have established the Live CLEM technology that can be applied to the study of floating cells such as S. cereviciae and S. pombe as well as of swimming cells such as Tetrahymena.
|
Report
(4 results)
Research Products
(158 results)
-
-
[Journal Article] Transient Focal Membrane Deformation Induced by Arginine-rich Peptides Leads to Their Direct Penetration into Cells2012
Author(s)
Hirose, H., Takeuchi, T., Osakada, H., Pujals, S., Katayama, S., Nakase, I., Kobayashi, S., Haraguchi, T., Futaki, S
-
Journal Title
Molecular Therapy
Volume: (in press)
Issue: 5
Pages: 984-993
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 高速イメージング2011
Author(s)
原口徳子
Organizer
第17回細胞生物学ワークショップ
Place of Presentation
情報通信研究機構、兵庫県
Year and Date
2011-08-12
Related Report
-
[Presentation] 生細胞イメージング2011
Author(s)
原口徳子
Organizer
第17回細胞生物学ワークショップ
Place of Presentation
情報通信研究機構、兵庫県
Year and Date
2011-08-11
Related Report
-
-
[Presentation] The Nuclear Envelope Structures are Required for Maintenance of the Micronucleus Formed by Loss of Centromere Functions of a Human Artificial Chromosome2011
Author(s)
T. Haraguchi, T. Koujin, H. Osakada, T. Kojidani, S. Kobayashi, M. Nakano, H. Masumoto, V. L. Larionov, W. C. Earnshaw, Y. Hiraoka
Organizer
Nuclear envelope disease and chromatin organization
Place of Presentation
Robinson College, Cambridge, UK
Year and Date
2011-07-14
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 生細胞イメージング2010
Author(s)
原口徳子
Organizer
第15回細胞生物学ワークショップ
Place of Presentation
未来ICT研究センター、兵庫県
Year and Date
2010-08-18
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Live CLEM : A new approach for observing molecular dynamics in high resolution2009
Author(s)
T. Haraguchi, T. Koujin, T. Kojidani, H. Osakada, F. Bunai, M. Iwamoto, S. Kobayashi, H. Asakawa, Y. Hiraoka
Organizer
6^<th> International Symposium on Electron Microscopy in Medicine and Biology 2009
Place of Presentation
Centennial Hall, Kobe University, Japan
Year and Date
2009-09-17
Related Report
-
-
-
-
[Presentation] 生細胞イメージング2009
Author(s)
原口徳子
Organizer
第13回細胞生物学ワークショップ
Place of Presentation
未来ICT研究センター、兵庫県
Year and Date
2009-08-19
Related Report
-
[Presentation] Live CLEM法2009
Author(s)
原口徳子、荒神尚子、糀谷知子、小坂田裕子
Organizer
第34回組織細胞化学講習会
Place of Presentation
徳島大学、徳島県
Year and Date
2009-07-30
Related Report
-
-
-
-
-
-
-
-
-
-
[Presentation] Live CLEM : A New Approach for Observing Molecular Dynamics in High Resolution2009
Author(s)
T. Haraguchi, T. Koujin, T. Kojidani, H. Osakada, S. Kobayashi, M. Iwamoto, F. Bunai, H. Asakawa, Y. Chikashige, Y. Hiraoka
Organizer
The 9th NIBB-EMBL Symposium "Functional Imaging from Atoms to Organisms"
Place of Presentation
Okazaki Conference Center, Japan
Year and Date
2009-04-20
Related Report
-
-
-
-
-
-
-
-
-
-