Presentation and suppression of axon guidance molecules by their receptors : a new principle of axon guidance

• Project/Area Number: 21370100
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute of Genetics
• Principal Investigator: HIROMI Yasushi 国立遺伝学研究所, 個体遺伝研究系, 教授 (70291888)
• Co-Investigator(Renkei-kenkyūsha): ASAOKA Miho 国立遺伝学研究所, 個体遺伝研究系, 助教 (40370118)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 軸索ガイダンス分子 / 軸索誘導 / ショウジョウバエ / ネトリン / Unc5 / Netrin / ガイダンス分子

Research Abstract

We analyzed how the distribution of an evolutionarily conserved axon guidance molecule Netrin could be influenced by its receptors. While the attractive receptor Frazzled/DCC presents Netrin on the axonal membrane, repulsive receptor Unc5 suppressed Netrin presentation. This suppressive activity exerted by pre-existing neurons creates a Netrin-less guidance field for the follower axons that are repelled by Netrin. We propose that a linkage between the guidance function of a receptor and its effect on ligand could be a new principle for axon guidance.

Research Products

• [Journal Article] Compartmentalization within neurites : its mechanisms and implications (2011)
  • Author(s): Katsuki T., Joshi R., Ailani D., Hiromi Y.
  • Journal Title: Dev Neurobiol Volume: 71 Issue: 6 Pages: 458-473
  • DOI: 10.1002/dneu.20859
  • Peer Reviewed
• [Journal Article] Intra-axonal patterning : intrinsic compartmentalization of the axonal membrane in Drosophila neurons (2009)
  • Author(s): Katsuki T., Ailani D., Hiramoto M., Hiromi Y.
  • Journal Title: Neuron Volume: 64 Issue: 2 Pages: 188-199
  • DOI: 10.1016/j.neuron.2009.08.019
  • Peer Reviewed
• [Journal Article] Midline governs axon pathfinding by coordinating expression of two major guidance systems (2009)
  • Author(s): Liu Q. X., Hiramoto M., Ueda H., Gojobori T., Hiromi Y., Hirose S.
  • Journal Title: Genes Dev Volume: 23 Issue: 10 Pages: 1165-70
  • DOI: 10.1101/gad.1774209
  • Peer Reviewed
• [Journal Article] Intra-axonal Patterning : Intrinsic Compartmentalization of the Axonal Membrane in Drosophila Neurons (2009)
  • Author(s): Katsuki, T.
  • Journal Title: Neuron 64 Pages: 188-199
  • Peer Reviewed
• [Journal Article] Midline governs axon pathfinding by coordinating expression of two major guidance systems (2009)
  • Author(s): Liu, QX
  • Journal Title: Genes & Development 23 Pages: 1165-1170
  • Peer Reviewed
• [Presentation] 極性からパターンへ:神経突起の区画化と神経回路形成 (2012)
  • Author(s): 広海健
  • Organizer: 生理学会合同シンポジウム「細胞極性化の原理と生理機能」第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 甲府
• [Presentation] Intra-axonal compartmentalizationof guidance receptors in drosophila neurons (2011)
  • Author(s): Joshi, R., Katsuki, T., DeFalco, T., Hiromi, Y.
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington, DC
• [Presentation] Intra-axonal compartmentalizationof guidance receptors in drosophila neurons (2011)
  • Author(s): Joshi, R., Katsuki, T., DeFalco, T., Hiromi, Y.
  • Organizer: Assembly, Plasticity, Dysfunction and Repair of Neural Circuits
  • Place of Presentation: Suzhou
• [Presentation] Intra-axonal compartmentalization of axon guidance receptors : its mechanism and roles in axon guidance (2011)
  • Author(s): Hiromi, Y.
  • Organizer: シンポジウム「Gradients of guidance cues in axon and dendrite patterning」第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Intra-axonal patterning (2011)
  • Author(s): Hiromi, Y.
  • Organizer: JSDB-GfE Joint Meeting
  • Place of Presentation: Dresden
• [Presentation] "Intra-axonal compartmentalization" of guidance receptors in drosophila neurons (2011)
  • Author(s): Joshi, R., Katsuki, T., DeFalco, T., Hiromi, Y.
  • Organizer: Assembly, Plasticity, Dysfunction and Repair of Neural Circuits
  • Place of Presentation: Suzhou
• [Presentation] "Intra-axonal compartmentalization" of guidance receptors in drosophila neurons (2011)
  • Author(s): Joshi, R., Katsuki, T., DeFalco, T., Hiromi, Y.
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington, DC
• [Presentation] Intra-axonal patterning (2011)
  • Author(s): Yasushi Hiromi
  • Organizer: JSDB-GfE Joint Meeting
  • Place of Presentation: Dresden, Germany
• [Presentation] Intra-axonal compartments : novel structural units of neural circuits? (2011)
  • Author(s): Yasushi Hiromi
  • Organizer: 第88回日本生理学会大会第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜
• [Presentation] Generation of pattern within the axonal membrane (2010)
  • Author(s): Hiromi, Y.
  • Organizer: The 13th International Membrane Research Forum
  • Place of Presentation: Kyoto
• [Presentation] Intra-axonal patterning : intrinsic compartmentalization of the axonal membrane in Drosophila neurons (2009)
  • Author(s): Katsuki, T., Ailani, D., Joshi, R., Defalco, T., Hiramoto, M., Hiromi Y.
  • Organizer: The 9th Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
• [Presentation] Molecular addresses : motifs involved in compartment-specific localization of guidance receptors in Drosophila axons (2009)
  • Author(s): Joshi, R., Katsuki, T., DeFalco, T., Hiromi, Y.
  • Organizer: The 9th Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
• [Presentation] Differential Trafficking Pathways Regulate the Localization of Axon Guidance Receptors in Drosophila neurons (2009)
  • Author(s): Ailani D., Katsuki T., Hiromi Y.
  • Organizer: The 9th Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/DevGen/hiromi.html
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/DevGen/hiromi.html
• [Remarks]
  • URL: http://www.nig.ac.jp/section/hiromi/hiromi-j.html
• [Remarks]
  • URL: http://www.nig.ac.jp/section/hiromi/hiromi-j.html