Significance of membrane/protein trafficking for the establishment of cell polarity in the vertebrate

• Project/Area Number: 21370102
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: UENO Naoto 基礎生物学研究所, 形態形成研究部門, 教授 (40221105)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Makoto (10533193)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2009: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 細胞極性 / タンパク質輸送 / 細胞骨格 / 原腸形成 / 初期発生

Research Abstract

We have demonstrated that for the establishment of cell polarity, stabilization and bundling of microtubule are essential and that molecules implicated in epithelial morphogenesis such as MID1 encoded by a causative gene of Opitz syndrome and its related protein MID2 play important role in the stabilization/bundling of microtubules. Thus we have been able to propose that this remodeling of microtubules allows the trafficking of secreted vesicles and cell polarity factors on the rail of bundled microtubules.

Research Products

• [Journal Article] Cell movements of the deep layer of non-neural ectoderm underlie complete neural tube closure in Xenopus (2012)
  • Author(s): Morita, H., Kajiura-Kobayashi, H., Takagi, C., Yamamoto, TS., Nonaka, S. and Ueno, N.
  • Journal Title: Development Volume: 139 Pages: 1417-1426
  • Peer Reviewed
• [Journal Article] Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis (2012)
  • Author(s): Tao, H., Inoue, K., Kiyonari, H., Bassuk, AG., Axelrod, JD., Sasaki, H., Aizawa, S. and Ueno, N.
  • Journal Title: Dev. Biol Volume: 364 Pages: 138-148
  • Peer Reviewed
• [Journal Article] Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis (2012)
  • Author(s): Tao, H., Inoue, K., Kiyonari, H., Bassuk, AG., Axelrod, JD., Sasaki, H., Aizawa, S., Ueno, N.
  • Journal Title: Dev.Biol. Volume: 364 Pages: 138-148
  • Peer Reviewed
• [Journal Article] MID1 and MID2 are required for Xenopus neural tube closure through the regu-lation of microtubule organization (2010)
  • Author(s): Suzuki, M., Hara, Y., Takagi, C., Yamamoto, TS. and Ueno, N.
  • Journal Title: Development Volume: 137 Pages: 2329-2339
  • Peer Reviewed
• [Journal Article] Nectin-2 and N-cadherin interact through extracellular domains and induce apical accumulation of F-actin in apical constriction of Xenopus neural tube morphogenesis (2010)
  • Author(s): Morita, H., Nandadasa, S., Yamamoto, TS., Terasaka-Iioka, C., Wylie, C., and Ueno, N.
  • Journal Title: Development Volume: 137 Pages: 1315-1325
  • Peer Reviewed
• [Journal Article] Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastru-lation (2010)
  • Author(s): Shindo, A., Hara, Y., Yamamoto, TS., Ohkura, M., Nakai, J. and Ueno, N.
  • Journal Title: Plos One Volume: 24
  • Peer Reviewed
• [Journal Article] N.Nectin-2 and N-cadherin interact through extracellular domains and induce apical accumulation of F-actin in apical constriction of Xenopus neural tube morphogenesis. (2010)
  • Author(s): Morita, H., Nandadasa, S., Yamamoto, T.S., Terasaka-Iioka, C., Wylie, C., Ueno, N.
  • Journal Title: Development Volume: 137(8) Pages: 1315-1325
  • Peer Reviewed
• [Journal Article] MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization. (2010)
  • Author(s): Suzuki, M., Hara, Y., Takagi, C., Yamamoto, T.S., Ueno, N.
  • Journal Title: Development Volume: 137 Pages: 2329-2339
  • Peer Reviewed
• [Journal Article] Tissue-tissue interaction triggered calcium elevation is required for cell polarization during Xenopus gastrulation (2010)
  • Author(s): Shindo, A., Hara, Y., Yamamoto, T.S., Ohkura, M., Nakai, J., Ueno, N.
  • Journal Title: PLoS One 5
  • Peer Reviewed
• [Journal Article] Nectin-2 and N-cadherin interact through extracellular domains and induce apical accumulation of F-actin in apical constriction of Xenopus neural tube morphogenesis (2010)
  • Author(s): Morita, H., Nandadasa, S., Yamamoto, T.S., Terasaka-Iioka, C., Wylie, C., Ueno
  • Journal Title: Development 137 Pages: 1315-1325
  • Peer Reviewed
• [Presentation] ゼブラフィッシュ神経管形成における非筋型ミオシンの時空間分布 (2011)
  • Author(s): 鈴木誠
  • Organizer: 発生生物学会第44回大会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-20
• [Presentation] Dissecting the contribution of non-neural ectoderm to the verte-brate neural tube closure (2011)
  • Author(s): 森田仁
  • Organizer: Joint Meeting of the German and Japanese Societies of Develop-mental Biologists
  • Place of Presentation: ドイツ・ハイデルベルグ
  • Year and Date: 2011-03-23
• [Presentation] Dissecting the contribution of non-neural ectoderm to the verte-brate neural tube closure (2010)
  • Author(s): 森田仁
  • Organizer: The 16th International Conference of the International Soiety of Differentiation
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2010-11-16
• [Presentation] Nectin-2 and N-cadherin Cooperatively Regulate Apical Const-riction in Xenopus Neural Tube Formation (2009)
  • Author(s): 森田仁
  • Organizer: 11th International EMBL PhD Symposium
  • Place of Presentation: ドイツ・ハイデルベルグ
  • Year and Date: 2009-10-31
• [Presentation] Nectin-2 and N-cadherin Cooperatively Regulate Apical Const-riction in Xenopus Neural Tube Formation (2009)
  • Author(s): 森田仁
  • Organizer: The ASCB/JSCB/RIKEN CDB Meeting ; Building the Body Plan : How Cell Adhesion, Signaling, and Cyto-skeletal Regulation Shape Morphogene-sis
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2009-09-21
• [Presentation] Regulation for Xenopus neural tube morphogenesis by nectin-2, an Ig-like adhesion molecule in cooperation with N-cadherin (2009)
  • Author(s): 森田仁
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2009-05-28