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Dorsal determination in vertebrate embryogenesis

Research Project

Project/Area Number 21370103
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNagoya University (2010-2011)
The Institute of Physical and Chemical Research (2009)

Principal Investigator

HIBI Masahiko  名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)

Co-Investigator(Kenkyū-buntansha) NOJIMA Hideaki  独立行政法人理化学研究所, 体軸形成研究チーム, 研究員 (00392069)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords胚葉形成・原腸形成・体節形成 / 体軸形成 / 背側決定 / 微小管 / モータータンパク質 / Wnt / 変異体 / ゼブラフィッシュ / アフリカツメガエル / モータータンパク / 蛍光タンパク質 / ライブイメージング / 初期 / 背側決定因子 / kinesin / Balbiani体 / 母性遺伝子効果 / 背側オーガナイザー
Research Abstract

In zebrafish embryos, dorsal determinants are believed to be initially localized to the vegetal pole and then transported to the prospective dorsal side of the embryos through a microtubule array. The dorsal determinants activate the canonical Wnt pathway and thereby promote the expression of genes that induce the dorsal organizer. We previously demonstrated that Syntabulin, a linker protein of kinesin I motor protein, is required for the dorsal organizer. We have found that the parallel microtubule array form at the cortical surface of the vegetal pole around 20 minutes after fertilization ; the plus end of microtubules is to the prospective dorsal side. We have also found that Syntabulin can interact with Grip2(glutamate receptor interacting protein 2). Our findings suggest that a complex of Syntabulin and Grip2 is involved in the microtubule-dependent transport of dorsal determinants.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (36 results)

All 2012 2011 2010 2009

All Journal Article (20 results) (of which Peer Reviewed: 12 results) Presentation (16 results)

  • [Journal Article] Development and evolution of cerebellar neural circuits2012

    • Author(s)
      Hashimoto, M. and Hibi, M.
    • Journal Title

      Dev. Growth Differ

      Volume: 54 Issue: 3 Pages: 373-389

    • DOI

      10.1111/j.1440-169x.2012.01348.x

    • NAID

      10031136910

    • Related Report
      2011 Final Research Report
  • [Journal Article] Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish2012

    • Author(s)
      Kuscha, V., Fraser, S. L., Dias, T. B., Hibi, M., Becker, T., and Becker, C. G.
    • Journal Title

      J. Comp. Neurol

      Volume: (in press) Issue: 16 Pages: 3604-3616

    • DOI

      10.1002/cne.23115

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution.2012

    • Author(s)
      Yuuta Moriyama
    • Journal Title

      Current Biology

      Volume: 21 Issue: 7 Pages: 601-607

    • DOI

      10.1016/j.cub.2012.01.063

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic changes in the gene expression of zebrafish Reelin receptors during embryogenesis and hatching period2012

    • Author(s)
      Hideaki Imai, Yoshihito Oomiya, Satoshi Kikkawa, Wataru Shoji, Masahiko Hibi, Toshio Terashima, Yu Katsuyama
    • Journal Title

      Development, Growth & Differentiation

      Volume: Vol.54 Issue: 2 Pages: 253-263

    • DOI

      10.1111/j.1440-169x.2012.01327.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of the cerebellum and cerebellar neural circuits2012

    • Author(s)
      Hibi, M. and Shimizu T.
    • Journal Title

      Dev. Neurobiol

      Volume: 72 Issue: 3 Pages: 282-301

    • DOI

      10.1002/dneu.20875

    • Related Report
      2011 Final Research Report
  • [Journal Article] Function of strawberry notch family genes in the zebrafish brain development2011

    • Author(s)
      Takano, A., Zochi, R., Hibi, M., Terashima, T., and Katsuyama, Y.
    • Journal Title

      Kobe. J. Med. Sci

      Volume: 56 Pages: 220-230

    • NAID

      110009587651

    • URL

      http://www.med.kobe-u.ac.jp/journal/contents/56/E220.pdf

    • Related Report
      2011 Final Research Report
  • [Journal Article] Development of the cerebellum and cerebellar neural circuit2011

    • Author(s)
      Hibi, M.
    • Journal Title

      Developmental Neurobiology

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Atypical PKC regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus2010

    • Author(s)
      Tanabe, K., Kani, S., Shimizu, T., Bae, Y.-K., Abe, T., and Hibi, M.
    • Journal Title

      J. Neurosci

      Volume: 30 Issue: 50 Pages: 16983-16992

    • DOI

      10.1523/jneurosci.3352-10.2010

    • Related Report
      2011 Final Research Report
  • [Journal Article] Dynein axonemal intermediate chain 2 is required for formation of the left-right body axis and kidney in medaka2010

    • Author(s)
      Nagao, Y., Cheng, J., Kamura, K., Seki, R., Maeda, A., Nihei, D., Koshida, S., Wakamatsu, Y., Fujimoto, T., Hibi, M., and Hashimoto, H.
    • Journal Title

      Dev. Biol.

      Volume: 347 Issue: 1 Pages: 53-61

    • DOI

      10.1016/j.ydbio.2010.08.001

    • Related Report
      2011 Final Research Report
  • [Journal Article] Expression of strawberry notch family genes during zebrafish embryogenesis2010

    • Author(s)
      Takano, A., Zochi, R., Hibi, M., Terashima, T., and Katsuyama, Y.
    • Journal Title

      Dev. Dyn.

      Volume: 239 Issue: 6 Pages: 1789-1796

    • DOI

      10.1002/dvdy.22287

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum2010

    • Author(s)
      Kani, S., Bae, Y.-K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M. J. Scott, E., Higashijima, S.-I., and Hibi, M.
    • Journal Title

      Dev. Biol.

      Volume: 343 Issue: 1-2 Pages: 1-17

    • DOI

      10.1016/j.ydbio.2010.03.024

    • Related Report
      2011 Final Research Report
  • [Journal Article] Zinc-finger genes Fezfl and Fezfl control neuronal differentiation by repressing Hes5 expression in forebrain2010

    • Author(s)
      Shimizu, T., Nakazawa, M., Kani, S., Bae, Y.-K., Shimizu, T., Kageyama, R., and Hibi, M.
    • Journal Title

      Development

      Volume: 137 Issue: 11 Pages: 1875-1885

    • DOI

      10.1242/dev.047167

    • Related Report
      2011 Final Research Report
  • [Journal Article] Syntabulin a motor protein linker controls dorsal determination.2010

    • Author(s)
      Nojima, H., Rothhamel, S., Shimizu, T., Kim, C. H., Yonemura, S., Marlow, F. L., Hibi, M.
    • Journal Title

      Development

      Volume: 137 Issue: 6 Pages: 923-933

    • DOI

      10.1242/dev.046425

    • Related Report
      2011 Final Research Report
  • [Journal Article] Zinc finger genes Fezf1 and Fezf2 control neuronal differentiation by repressin Hes5 expression in forebrain2010

    • Author(s)
      Shimizu, T.
    • Journal Title

      Development

      Volume: 137 Pages: 1875-1885

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum2010

    • Author(s)
      Kani, S.
    • Journal Title

      Developmental Biology

      Volume: 343 Pages: 1-17

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of strawberry notch family genes during zebrafish embryogenesis2010

    • Author(s)
      Takano, A.
    • Journal Title

      Developmental Dynamic

      Volume: 239 Pages: 1789-1796

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynein axonemal intermediate chain 2 is required for formation of the left-right body axis and kidney in medaka2010

    • Author(s)
      Nagao, Y.
    • Journal Title

      Developmental Biology

      Volume: 347 Pages: 53-61

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Atypical PKC regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus2010

    • Author(s)
      Tanabe, K.
    • Journal Title

      Journal of Neuroscience

      Volume: 30 Pages: 16983-16992

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Syntabulin, a motor protein linker, controls dorsal determination2010

    • Author(s)
      Nojima H, Rothhamel S, Shimizu T, Kim CH, Yonemura S, Marlow FL, Hibi M.
    • Journal Title

      Development 137

      Pages: 923-933

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anatomy of zebrafish cerebellum and screen for mutations affecting its development2009

    • Author(s)
      Bae YK, Kani S, Shimizu T, Tanabe K, Nojima H, Kimura Y, Higashijima S, Hibi M.
    • Journal Title

      Developmental Biology 330

      Pages: 406-426

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Genetic control for formation of cerebellar neural circuits in zebtafish and medaka2012

    • Author(s)
      Hibi, M.
    • Organizer
      GCOE International Symposium ; Formation and Function of Brain Networks : From Genes to Behaviors
    • Place of Presentation
      名古屋
    • Year and Date
      2012-01-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Signaling and gene cascades controlling embryonic polarity2011

    • Author(s)
      Hibi, M.
    • Organizer
      Karin Lab Symposium
    • Place of Presentation
      La Jolla, California, USA
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of cerebellar neural circuits in zebrafish and medaka2011

    • Author(s)
      Hibi, M.
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Genetic control of cerebellar neural circuits in zebrafish and medaka2011

    • Author(s)
      Hibi, M.
    • Organizer
      The 5^<th> Asia Oceania Zebrafish Meeting
    • Place of Presentation
      北京
    • Year and Date
      2011-08-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Microtubule-depedent dorsal determination in zebrafish2011

    • Author(s)
      Hibi, M.
    • Organizer
      第44回日本発生生物学会年会
    • Place of Presentation
      沖縄
    • Year and Date
      2011-05-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Microtubule-dependent dorsal determination in zebrafish2011

    • Author(s)
      Seki, R.
    • Organizer
      第44回日本発生生物学会年会
    • Place of Presentation
      沖縄
    • Year and Date
      2011-05-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Microtubule-Dependent Dorsal Determination in Zebrafish2011

    • Author(s)
      Hino, H.
    • Organizer
      第17回小型魚類研究会
    • Place of Presentation
      三島
    • Related Report
      2011 Final Research Report
  • [Presentation] Genetic Control of Cerebellar Neural Circuit Formation2011

    • Author(s)
      Hibi, M.
    • Organizer
      4^<th> Strategic Conference of Zebrafish Investigators
    • Place of Presentation
      Asilomar, California, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dendrite fommation of Purkinje cells in zebrafish cerebellum2010

    • Author(s)
      日比正彦
    • Organizer
      Neuro 2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Syntabulin a Motor Protein Linker, Controls Dorsal Determination.2010

    • Author(s)
      Hibi, M.
    • Organizer
      8th International Conference on Zebrfish Development and Genetics
    • Place of Presentation
      Madison, USA
    • Year and Date
      2010-06-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Excessive and Ectopic Development of Embryonic Leucophores in Medaka ml-3 Mutant is Caused by sox5 Mutation.2010

    • Author(s)
      Hashimoto, H.
    • Organizer
      8^<th> International Conference on Zebrfish Development and Genetics
    • Place of Presentation
      Madison, Wisconsin, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Syntabulin, a Motor Protein Linker, Controls Dorsal Determination2010

    • Author(s)
      Nojima, H.
    • Organizer
      8^<th> International Conference on Zebrfish Development and Genetics
    • Place of Presentation
      Madison, Wisconsin, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dynein Axonemal Intermediate Chain 2 is Required for Formation of the Left-right Body Axis and Kidney in Medaka2010

    • Author(s)
      Nagao, H.
    • Organizer
      8^<th> International Conference on Zebrfish Development and Genetics
    • Place of Presentation
      Madison, Wisconsin, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Neurogenesis in Zebrafish Cerebellum2010

    • Author(s)
      Shimizu, T.
    • Organizer
      8^<th> International Conference on Zebrfish Development and Genetics
    • Place of Presentation
      Madison, Wisconsin, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Syntabulin a linker of the motor protein, controls dorsal determin ation in zebrafish embryos2009

    • Author(s)
      野嶋秀明
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      (名古屋)(口頭)
    • Year and Date
      2009-09-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Role of Tokkaebi/Syntabulin-mediated transport in zebrafish dorsal determination2009

    • Author(s)
      Nojima, H.
    • Organizer
      第42回日本発生生物学会年会
    • Place of Presentation
      新潟
    • Year and Date
      2009-05-29
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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