Dorsal determination in vertebrate embryogenesis

• Project/Area Number: 21370103
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Nagoya University (2010-2011); The Institute of Physical and Chemical Research (2009)
• Principal Investigator: HIBI Masahiko 名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
• Co-Investigator(Kenkyū-buntansha): NOJIMA Hideaki 独立行政法人理化学研究所, 体軸形成研究チーム, 研究員 (00392069)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 胚葉形成・原腸形成・体節形成 / 体軸形成 / 背側決定 / 微小管 / モータータンパク質 / Wnt / 変異体 / ゼブラフィッシュ / アフリカツメガエル / モータータンパク / 蛍光タンパク質 / ライブイメージング / 初期 / 背側決定因子 / kinesin / Balbiani体 / 母性遺伝子効果 / 背側オーガナイザー

Research Abstract

In zebrafish embryos, dorsal determinants are believed to be initially localized to the vegetal pole and then transported to the prospective dorsal side of the embryos through a microtubule array. The dorsal determinants activate the canonical Wnt pathway and thereby promote the expression of genes that induce the dorsal organizer. We previously demonstrated that Syntabulin, a linker protein of kinesin I motor protein, is required for the dorsal organizer. We have found that the parallel microtubule array form at the cortical surface of the vegetal pole around 20 minutes after fertilization ; the plus end of microtubules is to the prospective dorsal side. We have also found that Syntabulin can interact with Grip2(glutamate receptor interacting protein 2). Our findings suggest that a complex of Syntabulin and Grip2 is involved in the microtubule-dependent transport of dorsal determinants.

Research Products

• [Journal Article] Development and evolution of cerebellar neural circuits (2012)
  • Author(s): Hashimoto, M. and Hibi, M.
  • Journal Title: Dev. Growth Differ Volume: 54 Issue: 3 Pages: 373-389
  • DOI: 10.1111/j.1440-169x.2012.01348.x
  • NAID: 10031136910
• [Journal Article] Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish (2012)
  • Author(s): Kuscha, V., Fraser, S. L., Dias, T. B., Hibi, M., Becker, T., and Becker, C. G.
  • Journal Title: J. Comp. Neurol Volume: (in press) Issue: 16 Pages: 3604-3616
  • DOI: 10.1002/cne.23115
  • Peer Reviewed
• [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution. (2012)
  • Author(s): Yuuta Moriyama
  • Journal Title: Current Biology Volume: 21 Issue: 7 Pages: 601-607
  • DOI: 10.1016/j.cub.2012.01.063
  • Peer Reviewed
• [Journal Article] Dynamic changes in the gene expression of zebrafish Reelin receptors during embryogenesis and hatching period (2012)
  • Author(s): Hideaki Imai, Yoshihito Oomiya, Satoshi Kikkawa, Wataru Shoji, Masahiko Hibi, Toshio Terashima, Yu Katsuyama
  • Journal Title: Development, Growth & Differentiation Volume: Vol.54 Issue: 2 Pages: 253-263
  • DOI: 10.1111/j.1440-169x.2012.01327.x
  • Peer Reviewed
• [Journal Article] Development of the cerebellum and cerebellar neural circuits (2012)
  • Author(s): Hibi, M. and Shimizu T.
  • Journal Title: Dev. Neurobiol Volume: 72 Issue: 3 Pages: 282-301
  • DOI: 10.1002/dneu.20875
• [Journal Article] Function of strawberry notch family genes in the zebrafish brain development (2011)
  • Author(s): Takano, A., Zochi, R., Hibi, M., Terashima, T., and Katsuyama, Y.
  • Journal Title: Kobe. J. Med. Sci Volume: 56 Pages: 220-230
  • NAID: 110009587651
  • URL: http://www.med.kobe-u.ac.jp/journal/contents/56/E220.pdf
• [Journal Article] Development of the cerebellum and cerebellar neural circuit (2011)
  • Author(s): Hibi, M.
  • Journal Title: Developmental Neurobiology
  • Peer Reviewed
• [Journal Article] Atypical PKC regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus (2010)
  • Author(s): Tanabe, K., Kani, S., Shimizu, T., Bae, Y.-K., Abe, T., and Hibi, M.
  • Journal Title: J. Neurosci Volume: 30 Issue: 50 Pages: 16983-16992
  • DOI: 10.1523/jneurosci.3352-10.2010
• [Journal Article] Dynein axonemal intermediate chain 2 is required for formation of the left-right body axis and kidney in medaka (2010)
  • Author(s): Nagao, Y., Cheng, J., Kamura, K., Seki, R., Maeda, A., Nihei, D., Koshida, S., Wakamatsu, Y., Fujimoto, T., Hibi, M., and Hashimoto, H.
  • Journal Title: Dev. Biol. Volume: 347 Issue: 1 Pages: 53-61
  • DOI: 10.1016/j.ydbio.2010.08.001
• [Journal Article] Expression of strawberry notch family genes during zebrafish embryogenesis (2010)
  • Author(s): Takano, A., Zochi, R., Hibi, M., Terashima, T., and Katsuyama, Y.
  • Journal Title: Dev. Dyn. Volume: 239 Issue: 6 Pages: 1789-1796
  • DOI: 10.1002/dvdy.22287
  • Peer Reviewed
• [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum (2010)
  • Author(s): Kani, S., Bae, Y.-K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M. J. Scott, E., Higashijima, S.-I., and Hibi, M.
  • Journal Title: Dev. Biol. Volume: 343 Issue: 1-2 Pages: 1-17
  • DOI: 10.1016/j.ydbio.2010.03.024
• [Journal Article] Zinc-finger genes Fezfl and Fezfl control neuronal differentiation by repressing Hes5 expression in forebrain (2010)
  • Author(s): Shimizu, T., Nakazawa, M., Kani, S., Bae, Y.-K., Shimizu, T., Kageyama, R., and Hibi, M.
  • Journal Title: Development Volume: 137 Issue: 11 Pages: 1875-1885
  • DOI: 10.1242/dev.047167
• [Journal Article] Syntabulin a motor protein linker controls dorsal determination. (2010)
  • Author(s): Nojima, H., Rothhamel, S., Shimizu, T., Kim, C. H., Yonemura, S., Marlow, F. L., Hibi, M.
  • Journal Title: Development Volume: 137 Issue: 6 Pages: 923-933
  • DOI: 10.1242/dev.046425
• [Journal Article] Zinc finger genes Fezf1 and Fezf2 control neuronal differentiation by repressin Hes5 expression in forebrain (2010)
  • Author(s): Shimizu, T.
  • Journal Title: Development Volume: 137 Pages: 1875-1885
  • Peer Reviewed
• [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum (2010)
  • Author(s): Kani, S.
  • Journal Title: Developmental Biology Volume: 343 Pages: 1-17
  • Peer Reviewed
• [Journal Article] Expression of strawberry notch family genes during zebrafish embryogenesis (2010)
  • Author(s): Takano, A.
  • Journal Title: Developmental Dynamic Volume: 239 Pages: 1789-1796
  • Peer Reviewed
• [Journal Article] Dynein axonemal intermediate chain 2 is required for formation of the left-right body axis and kidney in medaka (2010)
  • Author(s): Nagao, Y.
  • Journal Title: Developmental Biology Volume: 347 Pages: 53-61
  • Peer Reviewed
• [Journal Article] Atypical PKC regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus (2010)
  • Author(s): Tanabe, K.
  • Journal Title: Journal of Neuroscience Volume: 30 Pages: 16983-16992
  • Peer Reviewed
• [Journal Article] Syntabulin, a motor protein linker, controls dorsal determination (2010)
  • Author(s): Nojima H, Rothhamel S, Shimizu T, Kim CH, Yonemura S, Marlow FL, Hibi M.
  • Journal Title: Development 137 Pages: 923-933
  • Peer Reviewed
• [Journal Article] Anatomy of zebrafish cerebellum and screen for mutations affecting its development (2009)
  • Author(s): Bae YK, Kani S, Shimizu T, Tanabe K, Nojima H, Kimura Y, Higashijima S, Hibi M.
  • Journal Title: Developmental Biology 330 Pages: 406-426
  • Peer Reviewed
• [Presentation] Genetic control for formation of cerebellar neural circuits in zebtafish and medaka (2012)
  • Author(s): Hibi, M.
  • Organizer: GCOE International Symposium ; Formation and Function of Brain Networks : From Genes to Behaviors
  • Place of Presentation: 名古屋
  • Year and Date: 2012-01-12
• [Presentation] Signaling and gene cascades controlling embryonic polarity (2011)
  • Author(s): Hibi, M.
  • Organizer: Karin Lab Symposium
  • Place of Presentation: La Jolla, California, USA
  • Year and Date: 2011-11-19
• [Presentation] Development of cerebellar neural circuits in zebrafish and medaka (2011)
  • Author(s): Hibi, M.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] Genetic control of cerebellar neural circuits in zebrafish and medaka (2011)
  • Author(s): Hibi, M.
  • Organizer: The 5^<th> Asia Oceania Zebrafish Meeting
  • Place of Presentation: 北京
  • Year and Date: 2011-08-27
• [Presentation] Microtubule-depedent dorsal determination in zebrafish (2011)
  • Author(s): Hibi, M.
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Microtubule-dependent dorsal determination in zebrafish (2011)
  • Author(s): Seki, R.
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Microtubule-Dependent Dorsal Determination in Zebrafish (2011)
  • Author(s): Hino, H.
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 三島
• [Presentation] Genetic Control of Cerebellar Neural Circuit Formation (2011)
  • Author(s): Hibi, M.
  • Organizer: 4^<th> Strategic Conference of Zebrafish Investigators
  • Place of Presentation: Asilomar, California, USA
• [Presentation] Dendrite fommation of Purkinje cells in zebrafish cerebellum (2010)
  • Author(s): 日比正彦
  • Organizer: Neuro 2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] Syntabulin a Motor Protein Linker, Controls Dorsal Determination. (2010)
  • Author(s): Hibi, M.
  • Organizer: 8th International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, USA
  • Year and Date: 2010-06-18
• [Presentation] Excessive and Ectopic Development of Embryonic Leucophores in Medaka ml-3 Mutant is Caused by sox5 Mutation. (2010)
  • Author(s): Hashimoto, H.
  • Organizer: 8^<th> International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
• [Presentation] Syntabulin, a Motor Protein Linker, Controls Dorsal Determination (2010)
  • Author(s): Nojima, H.
  • Organizer: 8^<th> International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
• [Presentation] Dynein Axonemal Intermediate Chain 2 is Required for Formation of the Left-right Body Axis and Kidney in Medaka (2010)
  • Author(s): Nagao, H.
  • Organizer: 8^<th> International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
• [Presentation] Neurogenesis in Zebrafish Cerebellum (2010)
  • Author(s): Shimizu, T.
  • Organizer: 8^<th> International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, Wisconsin, USA
• [Presentation] Syntabulin a linker of the motor protein, controls dorsal determin ation in zebrafish embryos (2009)
  • Author(s): 野嶋秀明
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: (名古屋)(口頭)
  • Year and Date: 2009-09-12
• [Presentation] Role of Tokkaebi/Syntabulin-mediated transport in zebrafish dorsal determination (2009)
  • Author(s): Nojima, H.
  • Organizer: 第42回日本発生生物学会年会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-29