| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
In zebrafish embryos, dorsal determinants are believed to be initially localized to the vegetal pole and then transported to the prospective dorsal side of the embryos through a microtubule array. The dorsal determinants activate the canonical Wnt pathway and thereby promote the expression of genes that induce the dorsal organizer. We previously demonstrated that Syntabulin, a linker protein of kinesin I motor protein, is required for the dorsal organizer. We have found that the parallel microtubule array form at the cortical surface of the vegetal pole around 20 minutes after fertilization ; the plus end of microtubules is to the prospective dorsal side. We have also found that Syntabulin can interact with Grip2(glutamate receptor interacting protein 2). Our findings suggest that a complex of Syntabulin and Grip2 is involved in the microtubule-dependent transport of dorsal determinants.
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