Molecular Mechanism of neuroectodermal determination by zinc-finger proteins

• Project/Area Number: 21370104
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SASAI Yoshiki 独立行政法人理化学研究所, 器官発生研究グループ, グループディレクター (20283616)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 神経発生 / 細胞分化 / 転写因子 / 外胚葉 / 中胚葉 / ES細胞 / Znフィンガー因子 / 多能性幹細胞 / Zfp521 / Zfp12 / XFDL156 / 神経分化 / P300 / アフリカツメガエル / Xenopus / 発生 / 分化 / アニマルキャップ / 脳形成

Research Abstract

In this study, to understand the molecular mechanism underlying the neuroectodermal differentiation during early mammalian development, we analyzed the roles of two zinc-finger proteins that are involved in this process using in vitro differentiation system of pluripotent stem cells. We isolated mZfp12, a mammalian homolog of XFDL156, and showed that its overexpression in differentiating ES cells promotes neural differentiation and inhibits mesodermal differentiation. In addition, we iaolsted another zinc-finger protein Zfp521, and demonstrated its essential role in neuroectodermal differentiation from immature ectoderm.

Research Products

• [Journal Article] Mouse ES cell culture for generation of three-dimensional retinal and cortical tissues. (2012)
  • Author(s): Eiraku, M. and Sasai, Y.
  • Journal Title: Nature Protocols Volume: 7 Pages: 69-79
  • Peer Reviewed
• [Journal Article] Mouse ES cell culture for generation of three-dimensional retinal and cortical tissues (2012)
  • Author(s): Eiraku, M., Sasai, Y.
  • Journal Title: Nature Protocols Volume: 7 Issue: 1 Pages: 69-79
  • DOI: 10.1038/nprot.2011.429
  • Peer Reviewed
• [Journal Article] Self-organizing optic-cup morphogenesis in three-dimensional culture. (2011)
  • Author(s): Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K., Adachi, T. and Sasai, Y.
  • Journal Title: Nature Volume: 472 Pages: 51-56
  • Peer Reviewed
• [Journal Article] Lonely death dance of human pluripotent stem cells : ROCKing between metastable cell states (2011)
  • Author(s): Ohgushi, M. and Sasai, Y.
  • Journal Title: Trends in Cell Biology Volume: 21 Pages: 274-282
  • Peer Reviewed
• [Journal Article] Intrinsic transition of ES cell differentiation into neural progenitors. (2011)
  • Author(s): Kamiya, D., Banno, S., Sasai, N., Ohgushi, M., Watanabe, K., Kawada, M., Yakura, R., Jakt, L. M., Nishikawa, S. and Sasai. Y.
  • Journal Title: Nature Volume: 470 Pages: 503-509
  • Peer Reviewed
• [Journal Article] Self- organizing optic-cup morpho-genesis in three-dimensional culture (2011)
  • Author(s): M. Eiraku, N. Takata, H. Ishibashi, M. Kawada, E. Sakakura, S. Okuda, K. Sekiguchi, T. Adachi, Y. Sasai
  • Journal Title: Nature Volume: Vol. 472 Issue: 7341 Pages: 51-56
  • DOI: 10.1038/nature09941
  • Peer Reviewed
• [Journal Article] Jiraiya Attenuates BMP Signaling by Interfering with Type-II BMP Receptors in Neuroectodermal Patterning. (2010)
  • Author(s): Aramaki, T., Sasai, N., Yakura, R. and Sasai, Y.
  • Journal Title: Developmental Cell Volume: 19 Pages: 547-561
  • Peer Reviewed
• [Journal Article] Anterior neural development requires Del1, a matrix-associated protein that attenuates canonical Wnt signaling via the Ror2 pathway (2010)
  • Author(s): Takai, A., Inomata, H., Arakawa, A., Matsuo-Takasaki, M. and Sasai, Y.
  • Journal Title: Development Volume: 137 Pages: 3293-3302
  • Peer Reviewed
• [Journal Article] Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin. (2010)
  • Author(s): Eri Mizuhara, Yasuko Minaki, Tomoya Nakatani, Minoru Kumai, Takeshi Inoue, Keiko Muguruma, Yoshiki Sasai, and Yuichi Ono.
  • Journal Title: Developmental Biology Volume: 338 Pages: 202-214
  • Peer Reviewed
• [Journal Article] Anterior neural development requires Dell, a matrix-associated protein that attenuates canonical Wnt signaling via the Ror2 pathway (2010)
  • Author(s): Akira Takai, et al
  • Journal Title: Development Volume: 137 Pages: 3293-3302
  • Peer Reviewed
• [Journal Article] Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin (2010)
  • Author(s): Eri Mizuhara
  • Journal Title: Developmental Biology (in press)
  • Peer Reviewed
• [Journal Article] Pericellular matrix of decidua-derived mesenchymal cells : a potent human-derived substrate for the maintenance culture of human ES cells. (2009)
  • Author(s): Nagase T, Ueno M, Matsumura M, Muguruma K, Ohgushi M, Kondo N, Kanematsu D, Kanemura Y, Sasai Y.
  • Journal Title: Developmental Dynamics Volume: 238 Pages: 1118-1130
  • Peer Reviewed
• [Journal Article] In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction (2009)
  • Author(s): Osakada, F., Jin, ZB., Hirami, Y., Ikeda, H., Danjyo, T., Watanabe, K., Sasai, Y., Takahashi, M.
  • Journal Title: Journal of Cell Science Volume: 122 Pages: 3169-79
  • Peer Reviewed
• [Journal Article] Pericellular matrix of decidua-derived mesenchymal cells : a potent human-derived substrate for the maintenance culture of human ES cells (2009)
  • Author(s): Tomoko Nagase
  • Journal Title: Developmental Dynamics 238 Pages: 1118-1130
  • Peer Reviewed
• [Journal Article] In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction (2009)
  • Author(s): Fumitaka Osakada
  • Journal Title: Journal of Cell Science 122 Pages: 3169-3179
  • Peer Reviewed
• [Presentation] Self-organization of neural structures in 3D ES cell culture (2011)
  • Author(s): 笹井芳樹
  • Organizer: The Cambridge Neural Stem Cell Symposium
  • Place of Presentation: ケンブリッジ
  • Year and Date: 2011-09-05
• [Presentation] Self-organization of neural structures in 3D ES cell culture (2011)
  • Author(s): 笹井芳樹
  • Organizer: The Cambridge Neural Stem Cell Symposium, 2011
  • Place of Presentation: ケンブリッジ(イギリス)(招待講演)
  • Year and Date: 2011-09-05
• [Presentation] Induction and self-organization in neural development (2011)
  • Author(s): 笹井芳樹
  • Organizer: SFB488 Symposium
  • Place of Presentation: ハイデルベルク
  • Year and Date: 2011-02-24
• [Presentation] Molecular and cellular control of neuroectodermal development (2009)
  • Author(s): 笹井芳樹
  • Organizer: UCSF international symposium of neural stem cells
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2009-10-01
• [Presentation] Molecular and cellular control of neuroectodermal development (2009)
  • Author(s): 笹井芳樹
  • Organizer: UCSF international symposium of neural stem cells
  • Place of Presentation: アメリカ/サンフランシスコ
  • Year and Date: 2009-10-01
• [Remarks]
  • URL: http://www.cdb.riken.jp/sasai/
• [Remarks]
  • URL: http://cdb.riken,jp/sasai/