Molecular geneticimprovement of rice productivity by regulation of leaf senescence.

• Project/Area Number: 21380007
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Hiroshima University
• Principal Investigator: KUSABA Makoto 広島大学, 大学院・理学研究科, 教授 (20370653)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: stay-green / 葉老化制御 / イネ / 葉老化 / 突然変異体 / 育種学 / 遺伝学 / 植物 / 生理学 / 老化

Research Abstract

In this project, we tried to isolate rice stay-green genes NYC3, NYC4and FSG1. NYC3encoded a novel α/β hydrolase-fold family protein, which is thought to be an enzyme metabolizing chlorophyll. NYC4 was an ortholog of THYLAKOID FORMATION1 in Arabidopsis thaliana. Our analysis suggests that NYC4 plays an important role in degradation of photosystem II subunits. In addition, we successfully delimited the candidate region of FSG1within 200kb.

Research Products

• [Journal Article] NYC4, the rice ortholog of ArabidopsisTHF1,is involved in the degradation of chlorophyll-protein complexes during leaf senescence. (2013)
  • Author(s): Yamatani, H., Sato Y., Masuda, Y., Kato, Y., Morita, R., Fukunaga K., Nagamura, Y., Nishimura, M., Sakamoto, W., Tanaka, A. and Kusaba, M.
  • Journal Title: Plant J Volume: 74 Pages: 652-662
  • Peer Reviewed
• [Journal Article] NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll-protein complexes during leaf senescence. (2013)
  • Author(s): Yamatani, H.
  • Journal Title: Plant J. Volume: 74 Issue: 4 Pages: 652-662
  • DOI: 10.1111/tpj.12154
  • Peer Reviewed
• [Journal Article] Field transcriptome revealed critical developmental and physiological transitions involved in the expression of growth potential in japonica rice. (2011)
  • Author(s): Sato, Y., Antonio, B., Namiki, N., Motoyama, R., Sugimoto, K., Takehisa, H., Minami, H., Kamatsuki, K., Kusaba, M., Hirochika, H., Nagamura, Y.
  • Journal Title: BMC Plant Biol. Volume: 11 Pages: 10-24
  • Peer Reviewed
• [Journal Article] Defect in NON YELLOW COLORING 3, an α/βhydrolase-fold family protein, causes a stay green phenotype during leaf senescence in rice. (2009)
  • Author(s): Morita, R., Sato, Y., Masuda, Y., Nishimura, M., and Kusaba, M.
  • Journal Title: Plant J Volume: 59 Pages: 940-952
  • Peer Reviewed
• [Journal Article] A novel carotenoid derivative, lutein 3-acetate, accumulates in senescent leaves of rice. (2009)
  • Author(s): Kusaba, M.^*, Maoka, T., Morita, R., Takaichi, S.
  • Journal Title: Plant Cell Physiol. Volume: 50 Pages: 1573-1577
  • NAID: 10027342908
  • Peer Reviewed
• [Journal Article] Defect in NON YELLOW COLORING 3, an α/β hydrolase-fold family protein, causes a stay green phenotype during leaf senescence in rice. (2009)
  • Author(s): Morita, R., Sato, Y., Masuda, Y., Nishimura, M., Kusaba. M.
  • Journal Title: Plant J. Volume: 59 Pages: 940-952
  • Peer Reviewed
• [Journal Article] Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis. (2009)
  • Author(s): Horie, Y., Ito, H., Kusaba, M., Tanaka, R., Tanaka, A.
  • Journal Title: J.Biol.Chem.. Volume: 284 Pages: 17449-17456
  • Peer Reviewed
• [Presentation] イネstay-green遺伝子NYC4の単離と機能解析 (2012)
  • Author(s): 山谷浩史・佐藤豊・増田優・森田竜平・西村実・草場信
  • Organizer: 第121回日本育種学会
  • Place of Presentation: 2012.3.30
  • Year and Date: 2012-03-30
• [Presentation] イネ老化葉に現れる新規ルテイン誘導体・ルテイン3-アセテートの生成調節 (2009)
  • Author(s): 草場信・眞岡孝至・森田竜平・高市真一
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-09-25
• [Presentation] イネstay-green遺伝子NYC4の単離と機能解析
  • Author(s): 山谷浩史（草場信）
  • Organizer: 日本育種学会
  • Place of Presentation: 宇都宮大学
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/bio/SHOKUI/index.html
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/bio/SHOKUI/index.html