Elucidation of the mechanism for the translation and accumulation into the endoplasmic reticulum of rice prolamine

• Project/Area Number: 21380008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Kyushu University
• Principal Investigator: KUMAMARU Toshihiro 九州大学, (連合)農学研究科(研究院), 准教授 (00284555)
• Co-Investigator(Kenkyū-buntansha): KAWAGOE Yasushi 独立行政法人農業生物資源研究所, 植物科学研究領域, 主任研究員 (50355757) ; SATOH Hikaru 九州大学, 農学研究院, 教授 (70128031)
• Co-Investigator(Renkei-kenkyūsha): OGAWA Masahiro 山口県立大学, 共通教育機構, 教授 (10160772)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 貯蔵タンパク質 / 小胞体 / 分子シャペロン / 突然変異 / イネ

Research Abstract

i) For the proteins participating in the localization on the specific endoplasmic reticulum of prolamine mRNAs, the mutation lines detected SNPs within the corresponding genes were selected. ii) The recognition of specific termination codon by ESP1 protein was suggested. iii) It was clear the localization of prolamines in PB-I. iv) The mutation lines detected the SNPs within the gene encoding the molecular chaperon PDIL2-3 were selected. It was clear that PDIL2-3 promoted the formation of disulfide-bridge between prolamine molecules. v) The candidate responsible genes of the mutations for prolamine accumulation were identified.

Research Products

• [Journal Article] Genomics and breeding of Brassicaceae crops (2014)
  • Author(s): Hoai T. T. T., Matsusaka, H., Toyosawa, Y., Suu T. D., Satoh H., Kumamaru T.
  • Journal Title: Breeding Science Volume: 64 Issue: 1 Pages: 1-1
  • DOI: 10.1270/jsbbs.64.1
  • NAID: 130004146416
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Journal Article] Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam (2014)
  • Author(s): Hoai T. T. T., Suu T. D., Satoh H., Kumamaru T.
  • Journal Title: Plant Breeding Volume: (published on line) Issue: 3 Pages: 341-347
  • DOI: 10.1111/pbr.12159
  • Peer Reviewed
• [Journal Article] Influence of single-nucleotide polymorphisms in the gene encoding granule-bound starch synthase I on amylose content in Vietnamese rice cultivars. (2014)
  • Author(s): Hoai T. T. T., Matsusaka, H., Toyosawa, Y., Suu T. D., Satoh H., Kumamaru T.
  • Journal Title: Breeding Science Volume: 64
  • NAID: 130004146432
  • Peer Reviewed
• [Journal Article] A guanine nucleotide exchange factor for Rab5 proteins is essential for intracellular transport of the proglutelin from the Golgi apparatus to the protein storage vacuole in rice endosperm (2013)
  • Author(s): Masako Fukuda, Liuying Wen, Mio Satoh-Cruz, Yasushi Kawagoe, Yoshiaki Nagamura, Thomas W. Okita, Haruhiko Washida, Aya Sugino, Sonoko Ishino, Yoshizumi Ishino, Masahiro Ogawa, Mariko Sunada, Takashi Ueda, and Toshihiro Kumamaru
  • Journal Title: Plant Physiol. Volume: 162 Issue: 2 Pages: 663-674
  • DOI: 10.1104/pp.113.217869
  • Peer Reviewed
• [Journal Article] The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5'-flap endonuclease activity (2012)
  • Author(s): Yang, Y., et al
  • Journal Title: The Journal of Biochemistry Volume: 151 Issue: 3 Pages: 663-678
  • DOI: 10.1093/jb/mvr145
  • Peer Reviewed
• [Journal Article] The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5'-flap endonuclease activity (2012)
  • Author(s): Yang, Y., S.Ishino, T.Yamagami, T.Kumamaru, H.Satoh, Y.Ishino
  • Journal Title: The Journal of Biochemistry Volume: 151 Pages: 317-327
  • NAID: 10031166070
  • Peer Reviewed
• [Journal Article] The small GTPase Rab5a is essential for intracellular transport of proglutelin from Golgi apparatus to the protein storage vacuole and endosomal membrane organization in developing rice endosperm (2011)
  • Author(s): Fukuda M., M. Satoh-Cruz, L. Wen, A. J. Crofts, A. Sugino, H. Washida, T. W. Okita, M. Ogawa, Y. Kawagoe, M. Maeshima, T. Kumamaru
  • Journal Title: Plant Physiology Volume: 157 Issue: 2 Pages: 632-644
  • DOI: 10.1104/pp.111.180505
  • Peer Reviewed
• [Journal Article] Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant (2011)
  • Author(s): Ushijima, T., H. Matsusaka, H. Jikuya, M. Ogawa, H. Satoh, T. Kumamaru
  • Journal Title: Plant Science Volume: 181 Issue: 2 Pages: 125-131
  • DOI: 10.1016/j.plantsci.2011.04.011
  • NAID: 120005133137
  • Peer Reviewed
• [Journal Article] A role for the cysteine-rich 10 kDa prolamin in protein body I formation in rice (2011)
  • Author(s): Nagamine A., H. Matsusaka, T. Ushijima, Y. Kawagoe, M. Ogawa, T. W. Okita, T. Kumamaru
  • Journal Title: Plant and Cell Physiology Volume: 52(6) Issue: 6 Pages: 1003-1016
  • DOI: 10.1093/pcp/pcr053
  • NAID: 10029711849
  • Peer Reviewed
• [Journal Article] Distinct role of PDI and P5 sulfhydryl oxidoreductases in multiple pathways for oxidation of structurally diverse storage proteins in rice (2011)
  • Author(s): Onda Y, A. Nagamine, M. Sakurai, T. Kumamaru, M. Ogawa, Y. Kawagoe
  • Journal Title: Plant Cell Volume: 23 Issue: 1 Pages: 210-223
  • DOI: 10.1105/tpc.110.079509
  • Peer Reviewed
• [Journal Article] Protein disulfide isomerase like 1-1 participates in the maturation of proglutelin within endoplasmic reticulum in rice endosperm (2010)
  • Author(s): Satoh-Cruz M., A. J. Crofts, Y. Takemoto-Kuno, A. Sugino, H. Washida, N. Crofts, T. W. Okita, M. Ogawa, H. Satoh, and T. Kumamaru
  • Journal Title: Plant and Cell Physiology Volume: 51(9) Issue: 9 Pages: 1581-1593
  • DOI: 10.1093/pcp/pcq098
  • NAID: 10027352794
  • Peer Reviewed
• [Journal Article] Glup4 gene encodes small GTPase, Rab5a in rice (2010)
  • Author(s): Satoh-Cruz M., M. Fukuda, M. Ogawa, H. Satoh, and T. Kumamaru
  • Journal Title: Rice Genetics Newsletter Volume: 25 Pages: 48-49
  • Peer Reviewed
• [Journal Article] Characterization of the rice glup4 mutant suggests a role for the small GTPase Rab5 in the biosynthesis of carbon and nitrogen storage reserves in developing endosperm (2010)
  • Author(s): Doroshenk K. A., A. J. Crofts, H. Washida, M. Satoh-Cruz, N. Crofts, T. W. Okita, R. T. Morris, J. J. Wyrick, M. Fukuda, T. Kumamaru, and H. Satoh
  • Journal Title: Breeding Science Volume: 60 Issue: 5 Pages: 556-567
  • DOI: 10.1270/jsbbs.60.556
  • NAID: 130004146270
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Journal Article] Gene-gene interactions between mutants that accumulate abnormally high amounts of proglutelin in rice seed (2010)
  • Author(s): Ueda Y., M. Satoh-Cruz, H. Matsusaka, Y. Takemoto-Kuno, M. Fukuda, T. W. Okita, M. Ogawa, H. Satoh, T. Kumamaru
  • Journal Title: Breeding Science Volume: 60 Issue: 5 Pages: 568-574
  • DOI: 10.1270/jsbbs.60.568
  • NAID: 130004146271
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Journal Article] Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed (2010)
  • Author(s): Kumamaru T., Y. Uemura, Y. Inoue, Y. Takemoto, Sadar U. Siddiqui, M. Ogawa, I. Hara-Nishimura and H. Satoh
  • Journal Title: Plant and Cell Physiology Volume: 51 Issue: 1 Pages: 38-46
  • DOI: 10.1093/pcp/pcp165
  • NAID: 130006992651
  • Peer Reviewed
• [Journal Article] Protein disulfide isomerase like 1-1 participates in the maturation of proglutelin within endoplasmic reticulum in rice endosperm (2010)
  • Author(s): Satoh-Cruz M., A.J.Crofts, Y.Takemoto-Kuno, A.Sugino, H.Washida, N.Crofts, T.W.Okita, M Ogawa, H.Satoh, T.Kumamaru
  • Journal Title: Plant and Cell Physiology Volume: 51 Pages: 1581-1593
  • NAID: 10027352794
  • Peer Reviewed
• [Journal Article] Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed. (2010)
  • Author(s): Kumamaru T., Y.Uemura, Y.Inoue, Y.Takemoto, S.U.Siddiqui, M.Ogawa, I.Hara-Nishimura, H.Satoh.
  • Journal Title: Plant and Cell Physiology 51 Pages: 38-46
  • NAID: 130006992651
  • Peer Reviewed
• [Journal Article] ER membrane-localized oxidoreductase Ero1 is required for disulfide bond formation in the rice endosperm (2009)
  • Author(s): Onda Y., T. Kumamaru, Y. Kawagoe
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 106 Issue: 33 Pages: 14156-14161
  • DOI: 10.1073/pnas.0904429106
  • Peer Reviewed
• [Journal Article] ER membrane-localized oxidoreductase Ero1 is required for disulfide bond formation in the rice endosperm. (2009)
  • Author(s): Onda Y., T.Kumamaru, Y.Kawagoe.
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 14156-14161
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 熊丸敏博、小川雅広、佐藤光、Thomas W.Okita.
  • Journal Title: イネ種子貯蔵タンパク質集積の遺伝的制御機構. 種子の科学とバイオテクノロジー,(原田久也ら(種子生理生化学研究会)編)(学会出版センター) Pages: 56-59
• [Presentation] イネ貯蔵タンパク質の輸送・集積に関する調節機構解明 (2013)
  • Author(s): 熊丸 敏博
  • Organizer: 日本育種学会第8回九州育種談話会
  • Place of Presentation: 諫早市
  • Year and Date: 2013-09-26
• [Presentation] Identification and characterization of a novel RNA-binding protein involved in glutelin mRNA processing and localization (2013)
  • Author(s): Tian L., K. A. Doroshenk, T. Kumamaru, T. W. Okita
  • Organizer: Plant Biology
  • Place of Presentation: Providence, USA
  • Year and Date: 2013-07-21
• [Presentation] Identification of potential effectors for the small regulator Rab5 (2013)
  • Author(s): Nagamine A., T. Kumamaru, T. W. Okita
  • Organizer: Plant Biology
  • Place of Presentation: Providence, USA
  • Year and Date: 2013-07-21
• [Presentation] タンパク質の翻訳における終止コドン認識に関するイネESP1/eRF1の機能解析 (2013)
  • Author(s): 山城 憲子, 牛島 智一, 熊丸 敏博
  • Organizer: 日本育種学会第123回講演会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2013-03-28
• [Presentation] イネ種子におけるグルテリンの細胞内輸送に関与する GLUP7遺伝子の連鎖地図の構築 (2013)
  • Author(s): 北原真衣, 田代 康介,熊丸 敏博
  • Organizer: 日本育種学会第123回講演会
  • Place of Presentation: 東京農業大学（東京）
• [Presentation] タンパク質の翻訳における終止コドン認識に関するイネESP1/eRF1 の機能解析 (2013)
  • Author(s): 山城憲子, 牛島 智一, 熊丸 敏博
  • Organizer: 日本育種学会第123回講演会
  • Place of Presentation: 東京農業大学（東京）
• [Presentation] イネ胚乳におけるProtein Disulfide Isomerase Like (PDIL)2-3に関する変異体の選抜と解析 (2012)
  • Author(s): 豊澤佳子, 井上佳美, 吉川尚樹, 小川雅広, 熊丸敏博
  • Organizer: 日本育種学会第7回九州育種談話会
  • Place of Presentation: 九州沖縄農業研究センター
  • Year and Date: 2012-10-11
• [Presentation] GLUP6/GEF is the activator for GLUP4/Rab5a, and involved in intracellular transporting of proglutelin from Golgi to protein storage vacuole in rice endosperm (2012)
  • Author(s): M. Fukuda, L. Wen, M. Satoh-Cruz, Y. Kawagoe, T. W. Okita, M. Ogawa, M. Sunada, T. Ueda, T. Kumamaru
  • Organizer: Plant Biology
  • Place of Presentation: Austin, USA
  • Year and Date: 2012-07-21
• [Presentation] Identification of mutants for factors required for the ER-localization of prolamin-mRNAs in rice by TILLING method (2012)
  • Author(s): Kumamaru, T., Taniguchi, K., Fujimoto, W., Okita, T. W.
  • Organizer: Plant Biology
  • Place of Presentation: Austin, USA
  • Year and Date: 2012-07-21
• [Presentation] イネ胚乳におけるProtein Disulfide Isomerase Like (PDIL) 2-3に関する変異体の選抜と解析 (2012)
  • Author(s): 豊澤佳子, 井上佳美, Thomas W. Okita, 熊丸敏博
  • Organizer: イネ遺伝学・分子生物学ワークショップ
  • Place of Presentation: 奈良
  • Year and Date: 2012-07-06
• [Presentation] Construction of MNU-induced mutant pools and high performance TILLING system for detection of SNPs in rice (2012)
  • Author(s): Kumamaru T.
  • Organizer: BIT's 3rd Annual World, World DNA and Genome Day
  • Place of Presentation: 西安, 中国
  • Year and Date: 2012-04-26
• [Presentation] Detection of SNPs by TILLING and analysis of the gene function in rice (2012)
  • Author(s): Kumamaru, T.
  • Organizer: 第53回日本植物生理学会シンポジウム. Forefront of quality improvement and sophistication of plant bioresources
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] The relationship between Rab5a GTPase and guanine exchange factor in the intracellular transport of glutelin in rice seed (2012)
  • Author(s): Wen, L., M.Fukuda, M.Satoh-Cruz, T.W.Okita, M.Ogawa, T.Kumamaru
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] イネ胚乳においてsmall GTPase Rab5a はグルテリン前駆体のゴルジ体から貯蔵型液胞への細胞内輸送に関与する (2012)
  • Author(s): 福田真子, 佐藤美緒, 文柳瓔, A.J.Crofts, 杉野彩, 鷲田晴彦, T.W. Okita, 小川雅広, 川越靖, 前島正義, 熊丸敏博
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] Detection of SNPs by TILLING and analysis of the gene function in rice (2012)
  • Author(s): Kumamaru, T.
  • Organizer: 第53回日本植物生理学会シンポジウム
  • Place of Presentation: 京都産業大学(京都府)(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] Construction of MNU-induced mutant pools and high performance TILLING system for detection of SNPs in rice (2012)
  • Author(s): Kumamaru T.
  • Organizer: BIT’s 3rd Annual World, World DNA and Genome Day
  • Place of Presentation: 西安, 中国
  • Invited
• [Presentation] The small GTPase Rab5a is essential for intracellular transport of glutelin precursor from Golgi apparatus and endosomal membrane organization in developing rice endosperm. (2012)
  • Author(s): Fukuda M., L. Wen, M. Satoh-Cruz, T. W. Okita, Y. Kawagoe, M. Ogawa, T. Kumamaru
  • Organizer: BIT’s 3rd Annual World, World DNA and Genome Day
  • Place of Presentation: 西安, 中国
• [Presentation] GLUP6/GEF is the activator for GLUP4/Rab5a, and involved in intracellular transporting of proglutelin from Golgi to protein storage vacuole in rice endosperm (2012)
  • Author(s): FukudaM., L. Wen, M. Satoh-Cruz, Y. Kawagoe, T. W. Okita, M. Ogawa, M. Sunada, T. Ueda, T. Kumamaru
  • Organizer: Plant Biology 2012
  • Place of Presentation: Austin, USA
• [Presentation] Identification of mutants for factors required for the ER-localization of prolamin-mRNAs in rice by TILLING method (2012)
  • Author(s): Kumamaru T., Taniguchi K., Fujimoto W., Okita, T. W.
  • Organizer: Plant Biology 2012
  • Place of Presentation: Austin, USA
• [Presentation] イネ種子貯蔵タンパク質グルテリンのゴルジ体から貯蔵型液胞への細胞内輸送機構の解明 (2012)
  • Author(s): 福田真子, Thomas W. Okita, 熊丸敏博
  • Organizer: イネ遺伝学・分子生物学ワークショップ
  • Place of Presentation: 奈良
• [Presentation] 多様な高品質イネバイオリソースの整備に向けて (2012)
  • Author(s): 熊丸敏博, 安井秀, 吉村淳
  • Organizer: 日本遺伝学第84回大会「NBRPワークショップ： 九州ブランドのバイオリソース」
  • Place of Presentation: 九州大学（福岡）
• [Presentation] Guanine nucleotide exchange factor is required for the intracellular transport of proglutelins to protein storage vacuole in rice endosperm (2011)
  • Author(s): Fukuda, M., L. Wen, M. Satoh, Y. Kawagoe, T. W. Okita, M. Ogawa, T. Kumamaru
  • Organizer: American Society of Cell Biology
  • Place of Presentation: Denver, USA
  • Year and Date: 2011-12-04
• [Presentation] Guanine nucleotide exchange factor is required for the intracellular transport of proglutelins to protein storage vacuole in rice endosperm (2011)
  • Author(s): Fukuda, M., L.Wen, M.Satoh, Y.Kawagoe, T.W.Okita, M.Ogawa, T.Kumamaru
  • Organizer: American Society of Cell Biology
  • Place of Presentation: Denver Convention Center (Colorado, USA)
  • Year and Date: 2011-12-04
• [Presentation] The relationship between Rab5a GTPase and guanine nucleotide exchange factor in the intracellular transport of glutelin in rice seed (2011)
  • Author(s): Wen, L., M.Fukuda, M.Satoh-Cruz, T.W.Okita, M.Ogawa, T.Kumamaru
  • Organizer: American Society of Cell Biology
  • Place of Presentation: Denver Convention Center (Colorado, USA)
  • Year and Date: 2011-12-04
• [Presentation] イネプロラミンmRNA の特定小胞体への局在化に関する因子のTILLING 法を用いた突然変異体の選抜 (2011)
  • Author(s): 谷口晃一, 藤本若菜, T. W. Okita, 熊丸敏博
  • Organizer: 日本育種学会第6回九州育種談話会
  • Place of Presentation: 南九州大学
  • Year and Date: 2011-11-24
• [Presentation] イネプロラミンmRNAの特定小胞体への局在化に関する因子のTILLING法を用いた突然変異体の選抜 (2011)
  • Author(s): 谷口晃一、藤本若菜、T.W.Okita、熊丸敏博
  • Organizer: 日本育種学会第6回九州育種談話会
  • Place of Presentation: 南九州大学(宮崎県)
  • Year and Date: 2011-11-24
• [Presentation] イネα-グロブリンはPBI に一時的に局在する (2011)
  • Author(s): 小川雅広, 長嶺愛, T. W. Okita, 熊丸敏博
  • Organizer: 第32回種子生理生化学研究会年会
  • Place of Presentation: 日光
  • Year and Date: 2011-11-12
• [Presentation] イネ胚乳においてGEFはグルテリン前駆体の貯蔵型液胞への輸送に関与する (2011)
  • Author(s): 福田真子, 佐藤美緒, 川越靖, T.W.Okita, 小川雅広, 熊丸敏博
  • Organizer: 32回種子生理生化学研究会年会
  • Place of Presentation: 鬼怒川温泉緑水(栃木県)
  • Year and Date: 2011-11-12
• [Presentation] イネα-グロブリンはPBIに一時的に局在する (2011)
  • Author(s): 小川雅広・長嶺愛・T.W.Okita・熊丸敏博
  • Organizer: 32回種子生理生化学研究会年会
  • Place of Presentation: 鬼怒川温泉緑水(栃木県)
  • Year and Date: 2011-11-12
• [Presentation] MNU受精卵処理によるイネの種子成分に関する変異の作製 (2011)
  • Author(s): 佐藤 光, 西 愛子, 松坂弘明, 熊丸敏博
  • Organizer: 第32回種子生理生化学研究会年会
  • Place of Presentation: 日光
  • Year and Date: 2011-11-11
• [Presentation] MNU受精卵処理によるイネの種子成分に関する変異の作製 (2011)
  • Author(s): 佐藤光・西愛子・松坂弘明・熊丸敏博
  • Organizer: 第32回種子生理生化学研究会年会
  • Place of Presentation: 鬼怒川温泉緑水(栃木県)(招待講演)
  • Year and Date: 2011-11-11
• [Presentation] TILLING法を用いたイネOleosin変異体の選抜 (2011)
  • Author(s): 松坂弘明、佐藤匡夫、佐藤光、熊丸敏博
  • Organizer: 日本育種学会
  • Place of Presentation: 福井県立大学(福井県)
  • Year and Date: 2011-09-24
• [Presentation] イネプロラミンmRNAの特定小胞体への局在化に関する因子のTILLING 法を用いた突然変異体の選抜 (2011)
  • Author(s): 谷口晃一, 杉本若菜, T. W. Okita, 熊丸敏博
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学
  • Year and Date: 2011-09-23
• [Presentation] イネプロラミンmRNAの特定小胞体への局在化に関する因子のTILLING法を用いた突然変異体の選抜 (2011)
  • Author(s): 谷口晃一、藤本若菜、T.W.Okita、熊丸敏博
  • Organizer: 日本育種学会
  • Place of Presentation: 福井県立大学(福井県)
  • Year and Date: 2011-09-23
• [Presentation] The complexities of RNA localization in plant (2011)
  • Author(s): Okita T. W., K. A. Doroshenk, Y. Yang, N. Ozber, C. Wang, M. Satoh-Cruz, R. T. Morris, J. J. Wyrick, M. Ogawa, M. Fukuda, T. Kumamaru, H. Satoh, A. J. Crofts, N. Crofts, H. Washida
  • Organizer: Plant Biology
  • Place of Presentation: Mineapolis, USA
  • Year and Date: 2011-08-08
• [Presentation] The complexities of RNA localization in plant (2011)
  • Author(s): Okita T.W., K.A.Doroshenk,Y.Yang, N.Ozber, C.Wang, M.Satoh-Cruz, R.T.Morris, J.J.Wyrick, M.Ogawa, M.Fukuda, T.Kumamaru, H.Satoh, A.J.Crofts, N.Crofts, H.Washida
  • Organizer: Plant Biology 2011
  • Place of Presentation: Minneapolis convention center (Minnesota, USA)
  • Year and Date: 2011-08-08
• [Presentation] The ESP1 gene, regulatory factor of cysteine-poor prolamins, encode the eucaryotic peptide chain release factor 1(eRF1) in rice (2011)
  • Author(s): Ushijima, T., H. Matsusaka, Y. Kawagoe, M. Ogawa and T. Kumamaru
  • Organizer: International Plant RNA Workshop
  • Place of Presentation: 理化学研究所(横浜研究所)
  • Year and Date: 2011-06-21
• [Presentation] The ESP1 gene, regulatory factor of cysteine-poor prolamins, encode the eucaryotic peptide chain release factor 1(eRF1)in rice (2011)
  • Author(s): Ushijima, T., H.Matsusaka, Y.Kawagoe, M.Ogawa, T.Kumamaru
  • Organizer: International Plant RNA Workshop 2011
  • Place of Presentation: 理化学研究所(神奈川県)
  • Year and Date: 2011-06-21
• [Presentation] イネグルテリン前駆体を多量に蓄積するglup2変異遺伝子の連鎖地図の構築 (2011)
  • Author(s): 村上孝裕、野口亮介、小川雅広、熊丸敏博
  • Organizer: 日本育種学会119回講演会
  • Place of Presentation: 横浜市立大学(神奈川県)
  • Year and Date: 2011-03-30
• [Presentation] イネ胚乳におけるprotein disulfide isomerase like 2-3 (PDIL2-3)に関する変異体のTILLING 法を用いた選抜 (2011)
  • Author(s): 井上佳美, 吉川尚樹, 小川雅広, 熊丸敏博
  • Organizer: 日本育種学会119回講演会
  • Place of Presentation: 横浜市立大学
  • Year and Date: 2011-03-29
• [Presentation] イネ胚乳におけるprotein disulfide isomerase like 2-3 (PDIL2-3)に関する変異体のTILLING法を用いた選抜 (2011)
  • Author(s): 井上佳美、吉川尚樹、小川雅広、熊丸敏博
  • Organizer: 日本育種学会119回講演会
  • Place of Presentation: 横浜市立大学(神奈川県)
  • Year and Date: 2011-03-29
• [Presentation] イネ種子貯蔵タンパク質プロラミンの集積におけるCys-richプロラミンの作用 (2011)
  • Author(s): 長嶺愛, 松坂弘明, 川越靖, 小川雅広, T.W.Okita, 熊丸敏博
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2011-03-22
• [Presentation] イネ胚乳においてGEFはグルテリン前駆体の貯蔵型液胞への輸送に関与する (2011)
  • Author(s): 福田真子, 佐藤美緒, 川越靖, Thomas W.Okita, 小川雅広, 熊丸敏博
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2011-03-20
• [Presentation] The relationship between Rab5a GTPase and guanidine exchange factor involved in the intracellular transport of the glutelin in rice seed (2011)
  • Author(s): Wen, L., M.Fukuda, M.Satoh-Cruz, T.Kumamaru, M.Ogawa, T.W.Okita
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2011-03-20
• [Presentation] イネ種子貯蔵タンパク質プロラミンの集積におけるCys-rich プロラミンの作用 (2011)
  • Author(s): 長嶺愛, 松坂弘明, 川越靖, 小川雅広, T. W. Okita, 熊丸敏博
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 東北大学
• [Presentation] The relationship between Rab5a GTPase and guanidine exchange factor involved in the intracellular transport of the glutelin in rice seed (2011)
  • Author(s): Wen, L., M. Fukuda, M. Satoh-Cruz, T. Kumamaru, M. Ogawa, T. W. Okita
  • Organizer: 第52回植物生理学会年会
  • Place of Presentation: 東北大学
• [Presentation] イネ胚乳貯蔵オルガネラ発達の分子機構の解明 (2010)
  • Author(s): 川越靖, 長嶺愛, 恩田弥生, Yun Min-Soo, 熊丸敏博
  • Organizer: 第31回種子生理生化学研究会年会
  • Place of Presentation: 近江八幡
  • Year and Date: 2010-12-17
• [Presentation] イネ胚乳貯蔵オルガネラ発達の分子機構の解明 (2010)
  • Author(s): 川越靖, 長嶺愛, 恩田弥生, Yun Min-Soo, 熊丸敏博
  • Organizer: 第31回種子生理生化学研究会年会
  • Place of Presentation: 休暇村近江八幡(滋賀県)
  • Year and Date: 2010-12-17
• [Presentation] イネ種子におけるProtein Disulfide Isomerase (PDI) に関する変異体の選抜と解析 (2010)
  • Author(s): 井上佳美, 長嶺愛, 熊丸敏博, 小川雅広
  • Organizer: 日本育種学会第5回九州育種談話会
  • Place of Presentation: 佐賀大学
  • Year and Date: 2010-11-19
• [Presentation] イネグルテリン前駆体の小胞体からの輸送に関与するglup2変異遺伝子の連鎖地図構築 (2010)
  • Author(s): 村上孝裕、熊丸敏博、小川雅広
  • Organizer: 日本育種学会第5回九州育種談話会
  • Place of Presentation: 佐賀大学農学部(佐賀県)
  • Year and Date: 2010-11-19
• [Presentation] イネ種子におけるProtein Disulfide Isomerase (PDI)に関する変異体の選抜と解析 (2010)
  • Author(s): 井上佳美、長嶺愛、熊丸敏博、小川雅広
  • Organizer: 日本育種学会第5回九州育種談話会
  • Place of Presentation: 佐賀大学農学部(佐賀県)
  • Year and Date: 2010-11-19
• [Presentation] 液胞プロセッシング酵素はイネ種子グルテリンの結晶構造形成に寄与する (2010)
  • Author(s): 熊丸敏博, 井上佳美, 西村いくこ, 竹本陽子, 小川雅広, 佐藤光
  • Organizer: 第51回植物生理学会年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-18
• [Presentation] イネ貯蔵タンパク質mRNAの特定小胞体への局在化に関与するTudor-SNに関する突然変異体の選抜と解析 (2009)
  • Author(s): 藤本若菜, T. W. Okita, 佐藤美緒, 熊丸敏博
  • Organizer: 日本育種学会第4回九州育種談話会
  • Place of Presentation: 九州沖縄農業研究センター
  • Year and Date: 2009-12-11
• [Presentation] イネ貯蔵タンパク質mRNAの特定小胞体への局在化に関与するTudor-SNに関する突然変異体の選抜と解析 (2009)
  • Author(s): 藤本若菜、T.W.Okita、佐藤美緒、熊丸敏博
  • Organizer: 日本育種学会第4回九州育種談話会
  • Place of Presentation: 九州沖縄農業研究センター(合志)
  • Year and Date: 2009-12-11
• [Presentation] The small GTPase Rab5a is essential for intracellular transport of glutelin precursor from Golgi apparatus and endosomal membrane organization in developing rice endosperm (2009)
  • Author(s): Fukuda M., M.Satoh, H.Washida, A.Sugino, A.J.Crofts, T.W.Okita, Y.Kawagoe, M.Oeawa, H.Satoh, T.Kumamaru
  • Organizer: 日本育種学会第4回九州育種談話会
  • Place of Presentation: 九州沖縄農業研究センター(合志)
  • Year and Date: 2009-12-11
• [Presentation] MNUミュータジェネシスによるイネリソースの高品質化 (2009)
  • Author(s): 熊丸敏博・松坂弘明・佐藤光・倉田のり
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-12-09
• [Presentation] 高システインイネプロラミンの生合成・集積に関する遺伝子のマッピング (2009)
  • Author(s): 松坂弘明, 熊丸敏博, 佐藤光
  • Organizer: 第30回種子生理生化学研究会
  • Place of Presentation: 九州大学
  • Year and Date: 2009-11-27
• [Presentation] イネ低システインプロラミンの生合成に関わるEsp1遺伝子の単離 (2009)
  • Author(s): 牛島智一, 川越靖, 小川雅広, 佐藤光, 熊丸敏博
  • Organizer: 第30回種子生理生化学研究会
  • Place of Presentation: 九州大学
  • Year and Date: 2009-11-27
• [Presentation] イネ種子13 kD Cys-poor プロラミンの小胞体内への蓄積における10kD Cys-richプロラミンの役割 (2009)
  • Author(s): 長嶺 愛, 熊丸 敏博, 川越 靖
  • Organizer: 第30回種子生理生化学研究会
  • Place of Presentation: 九州大学
  • Year and Date: 2009-11-26
• [Presentation] The role of cysteine-rich prolamines for the formation of prolamine protein body in rice (2009)
  • Author(s): Nagamine A., Inoue Y., Ogawa M., Matsusaka H., Ushijima T., Satoh H., Okita T. W, Kumamaru T
  • Organizer: Plant Biology
  • Place of Presentation: Honolulu, USA
• [Presentation] Vacuolar processing enzyme plays an essential role in the formation of the glutelin crystalline structure in rice seed (2009)
  • Author(s): Kumamaru T., Y. Takemoto, I. Hara-Nishimura, M. Ogawa, H. Satoh
  • Organizer: Plant Biology
  • Place of Presentation: Honolulu, USA
• [Presentation] Vacuolar processing enzyme plays an essential role in the formation of the glutelin crystalline structure in rice seed (2009)
  • Author(s): Kumamaru T., Y.Takemoto, I.Hara-Nishimura, M.Ogawa, H.Satoh
  • Organizer: Plant Biology 2009
  • Place of Presentation: ハワイコンベンションセンター(ホノルル)
• [Presentation] The small GTPase Rab5a is essential for intracellular transport of glutelin precursor from Golgi apparatus and endosomal membrane organization in developing rice endosperm (2009)
  • Author(s): Fukuda M., M.Satoh, H.Washida, T.W.Okita, Y.Kawagoe, M.Ogawa, H.Satoh, T.Kumamaru
  • Organizer: Plant Biology 2009
  • Place of Presentation: ハワイコンベンションセンター(ホノルル)
• [Presentation] The role of cysteine-rich prolamines for the formation of prolamine protein body in rice (2009)
  • Author(s): Nagamine, A., Y.Inoue, M.Ogawa, H.Matsusaka, T.Ushijima, H.Satoh, T.W.Okita, T.Kumamaru,
  • Organizer: Plant Biology 2009
  • Place of Presentation: ハワイコンベンションセンター(ホノルル)
• [Presentation] Identification and characterization of a novel RNA-binding protein involved in glutelin mRNA processing and localization.
  • Author(s): Tian L., K. A. Doroshenk, T. Kumamaru, T. W. Okita.
  • Organizer: Plant Biology 2013
  • Place of Presentation: Providence, USA
• [Presentation] Identification of potential effectors for the small regulator Rab5.
  • Author(s): Nagamine A., T. Kumamaru, T. W. Okita.
  • Organizer: Plant Biology 2013
  • Place of Presentation: Providence, USA
• [Presentation] Rab5/GEF system is essential for intracellular transport of proglutelin from the Golgi apparatus to the protein storage vacuole in rice endosperm.
  • Author(s): Wen L., M. Fukuda, M. Ogawa, T.W. Okita, M. Sunada,T. Ueda, T. Kumamaru.
  • Organizer: Plant Biology 2013
  • Place of Presentation: Providence, USA
• [Presentation] イネ貯蔵タンパク質の輸送・集積に関する調節機構解明.
  • Author(s): 熊丸 敏博
  • Organizer: 日本育種学会第8回九州育種談話会
  • Place of Presentation: 諫早市
  • Invited
• [Presentation] Rab5/GEF system is essential for intracellular transport of proglutelin from the Golgi apparatus to the protein storage vacuole in rice
  • Author(s): Wen L., M. Fukuda, M. Ogawa, T. W. Okita, M. Sunada, T. Ueda, T. Kumamaru
  • Organizer: 日本育種学会第8回九州育種談話会
  • Place of Presentation: 諫早市
• [Presentation] イネ胚乳におけるProtein Disulfide Isomerase Like (PDIL)2-3に関する変異体の選抜と解析
  • Author(s): 豊澤佳子, 井上佳美, 吉川尚樹, 小川雅広, 熊丸敏博
  • Organizer: 日本育種学会第7回九州育種談話会
  • Place of Presentation: 九州沖縄農業研究センター(筑後）
• [Presentation] イネ胚乳においてGLUP6/GEFはGLUP4/Rab5aの活性化因子であり、グルテリン前駆体のゴルジ体から貯蔵型液胞への輸送に関与している
  • Author(s): 福田真子, 文柳瓔, 佐藤-Cruz美緒, T.W. Okita, 川越靖, 小川雅広, 砂田麻里子, 上田貴志, 熊丸敏博
  • Organizer: 日本育種学会第7回九州育種談話会
  • Place of Presentation: 九州沖縄農業研究センター（筑後）
• [Book] mRNA localization in plants and the role of RNA binding proteins. In RNA Binding Proteins (2012)
  • Author(s): Doroshenk K. A., A. J. Crofts, H. Washida, M. Satoh-Cruz, N. Crofts, Y. Yang, R. T. Morris, T. W. Okita, M. Fukuda, T. Kumamaru, H. Satoh
  • Publisher: Landes Bioscience
  • URL: http://www.landesbioscience.com/curie/chapter/5034/
• [Book] RNA Binding Proteins (2012)
  • Author(s): Doroshenk K. A.., A. J. Crofts, H. Washida, M. Satoh-Cruz, N. Crofts, Y. Yang, R. T. Morris, T. W. Okita, M. Fukuda, T. Kumamaru, H. Satoh
  • Total Pages: 18
  • Publisher: Landes Bioscience
• [Book] イネ種子貯蔵タンパク質集積の遺伝的制御機構. 種子の科学とバイオテクノロジー, 原田久也ら(種子生理生化学研究会)編 (2009)
  • Author(s): 熊丸敏博, 小川雅広, 佐藤光, Thomas W. Okita
  • Publisher: 学会出版センター